Result

Search results for the GEO ID: GSE43653

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GSM ID

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Title

Source name

Description

Characteristics

GSM1067632

GPL570

ECC-1 cell, control group rep1

Solvent(DMSO) treated ECC-1

cell line: endometrial cancer cell line (ECC-1)

DEHP C_1

Sample_geo_accession	GSM1067632
Sample_status	Public on Jan 23 2013
Sample_submission_date	Jan 22 2013
Sample_last_update_date	Jan 23 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	ECC-1 cells were seeded in a 100-mm dish at a density of 1.24 × 106 cells/ml. After incubation for 24 h at 37 °C, the cells were treated with 50 μM (The 20% inhibitory concentration (IC20)) di-(2-ethylhexyl) phthalate (DHEP) for 48 h.
Sample_growth_protocol_ch1	The ECC-1 cell line was donated from St. Vincent Hospital of Catholic University Medical college, Suwon, and was maintained under a humidified atmosphere of 5% CO2 and 95% air at 37℃. The culture medium was RPMI supplemented with 5% fetal bovine serum (GIBCO, Invitrogen Co., USA) and penicillin . The culture medium was refreshed every 2 to 3 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	hyunhee,,cho
Sample_contact_email	drrabbit@catholic.ac.kr
Sample_contact_institute	catholic university medical colleage
Sample_contact_address	seochogu banpodong
Sample_contact_city	seoul
Sample_contact_zip/postal_code	156080
Sample_contact_country	South Korea
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1067nnn/GSM1067632/suppl/GSM1067632_C-1_HG-U133_Plus_2_.CEL.gz
Sample_series_id	GSE43653
Sample_data_row_count	54613

GSM1067633

GPL570

ECC-1 cell, control group rep2

Solvent(DMSO) treated ECC-1

cell line: endometrial cancer cell line (ECC-1)

DEHP C_2

Sample_geo_accession	GSM1067633
Sample_status	Public on Jan 23 2013
Sample_submission_date	Jan 22 2013
Sample_last_update_date	Jan 23 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	ECC-1 cells were seeded in a 100-mm dish at a density of 1.24 × 106 cells/ml. After incubation for 24 h at 37 °C, the cells were treated with 50 μM (The 20% inhibitory concentration (IC20)) di-(2-ethylhexyl) phthalate (DHEP) for 48 h.
Sample_growth_protocol_ch1	The ECC-1 cell line was donated from St. Vincent Hospital of Catholic University Medical college, Suwon, and was maintained under a humidified atmosphere of 5% CO2 and 95% air at 37℃. The culture medium was RPMI supplemented with 5% fetal bovine serum (GIBCO, Invitrogen Co., USA) and penicillin . The culture medium was refreshed every 2 to 3 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	hyunhee,,cho
Sample_contact_email	drrabbit@catholic.ac.kr
Sample_contact_institute	catholic university medical colleage
Sample_contact_address	seochogu banpodong
Sample_contact_city	seoul
Sample_contact_zip/postal_code	156080
Sample_contact_country	South Korea
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1067nnn/GSM1067633/suppl/GSM1067633_C-2_HG-U133_Plus_2_.CEL.gz
Sample_series_id	GSE43653
Sample_data_row_count	54613

GSM1067634

GPL570

ECC-1 cell, treated with DEHP rep1

DEHP treated ECC-1

cell line: endometrial cancer cell line (ECC-1)

DEHP T_1

Sample_geo_accession	GSM1067634
Sample_status	Public on Jan 23 2013
Sample_submission_date	Jan 22 2013
Sample_last_update_date	Jan 23 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	ECC-1 cells were seeded in a 100-mm dish at a density of 1.24 × 106 cells/ml. After incubation for 24 h at 37 °C, the cells were treated with 50 μM (The 20% inhibitory concentration (IC20)) di-(2-ethylhexyl) phthalate (DHEP) for 48 h.
Sample_growth_protocol_ch1	The ECC-1 cell line was donated from St. Vincent Hospital of Catholic University Medical college, Suwon, and was maintained under a humidified atmosphere of 5% CO2 and 95% air at 37℃. The culture medium was RPMI supplemented with 5% fetal bovine serum (GIBCO, Invitrogen Co., USA) and penicillin . The culture medium was refreshed every 2 to 3 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	hyunhee,,cho
Sample_contact_email	drrabbit@catholic.ac.kr
Sample_contact_institute	catholic university medical colleage
Sample_contact_address	seochogu banpodong
Sample_contact_city	seoul
Sample_contact_zip/postal_code	156080
Sample_contact_country	South Korea
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1067nnn/GSM1067634/suppl/GSM1067634_T-1_HG-U133_Plus_2_.CEL.gz
Sample_series_id	GSE43653
Sample_data_row_count	54613

GSM1067635

GPL570

ECC-1 cell, treated with DEHP rep2

DEHP treated ECC-1

cell line: endometrial cancer cell line (ECC-1)

DEHP T_2

Sample_geo_accession	GSM1067635
Sample_status	Public on Jan 23 2013
Sample_submission_date	Jan 22 2013
Sample_last_update_date	Jan 23 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	ECC-1 cells were seeded in a 100-mm dish at a density of 1.24 × 106 cells/ml. After incubation for 24 h at 37 °C, the cells were treated with 50 μM (The 20% inhibitory concentration (IC20)) di-(2-ethylhexyl) phthalate (DHEP) for 48 h.
Sample_growth_protocol_ch1	The ECC-1 cell line was donated from St. Vincent Hospital of Catholic University Medical college, Suwon, and was maintained under a humidified atmosphere of 5% CO2 and 95% air at 37℃. The culture medium was RPMI supplemented with 5% fetal bovine serum (GIBCO, Invitrogen Co., USA) and penicillin . The culture medium was refreshed every 2 to 3 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	hyunhee,,cho
Sample_contact_email	drrabbit@catholic.ac.kr
Sample_contact_institute	catholic university medical colleage
Sample_contact_address	seochogu banpodong
Sample_contact_city	seoul
Sample_contact_zip/postal_code	156080
Sample_contact_country	South Korea
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1067nnn/GSM1067635/suppl/GSM1067635_T-2_HG-U133_Plus_2_.CEL.gz
Sample_series_id	GSE43653
Sample_data_row_count	54613

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