Result

Search results for the GEO ID: GSE43701

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM1068623

GPL570

HeLa cells-control

Human cervical squamous cell carcinoma cell line HeLa

cell line: HeLa treatment: none gender: female age: 31 years tissue: cervix life span: infinite morphology: epithelial-like

HeLa cells (control)

Sample_geo_accession	GSM1068623
Sample_status	Public on Jan 24 2013
Sample_submission_date	Jan 23 2013
Sample_last_update_date	Jan 24 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	MHT treatment was carried out by immersing plastic culture vessels containing the attached cells in a water bath at 41°C for 30 min. After heat treatment, the cells were incubated for 0-3 h at 37°C.
Sample_growth_protocol_ch1	HeLa cells were cultured in Dulbecco's Modified Eagle’s Medium supplemented with 10% fetal bovine serum at 37°C in humidified air with 5% CO2 and 95% air.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from cells using an RNeasy Total RNA Extraction kit (Qiagen), and treated with DNase I (Qiagen) for 15 min at room temperature to remove residual genomic DNA. RNA quality was analyzed using a Bioanalyzer 2100 (Agilent Technologies), producing RIN values between 9.5 and 10.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	500 ng of total RNA was used to synthesize cRNA with a GeneChip 3’ IVT Express Kit (Affymetrix).
Sample_hyb_protocol	After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
Sample_scan_protocol	The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
Sample_data_processing	GeneChip Analysis Suite
Sample_platform_id	GPL570
Sample_contact_name	Yoshiaki,,Tabuchi
Sample_contact_email	ytabu@cts.u-toyama.ac.jp
Sample_contact_laboratory	Division of Molecular Genetics Research
Sample_contact_department	Life Science Research Center
Sample_contact_institute	University of Toyama
Sample_contact_address	2630 Sugitani
Sample_contact_city	Toyama
Sample_contact_state	Toyama
Sample_contact_zip/postal_code	930-0194
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1068nnn/GSM1068623/suppl/GSM1068623_HeLa-C.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1068nnn/GSM1068623/suppl/GSM1068623_HeLa-C.CHP.gz
Sample_series_id	GSE43701
Sample_data_row_count	54675

GSM1068624

GPL570

HeLa cells-MHT 0h

Human cervical squamous cell carcinoma cell line HeLa

cell line: HeLa treatment: heat stress at 41°C and cultured for 0 h gender: female age: 31 years tissue: cervix life span: infinite morphology: epithelial-like

HeLa cells treated with heat stress at 41°C and cultured for 0 h

Sample_geo_accession	GSM1068624
Sample_status	Public on Jan 24 2013
Sample_submission_date	Jan 23 2013
Sample_last_update_date	Jan 24 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	MHT treatment was carried out by immersing plastic culture vessels containing the attached cells in a water bath at 41°C for 30 min. After heat treatment, the cells were incubated for 0-3 h at 37°C.
Sample_growth_protocol_ch1	HeLa cells were cultured in Dulbecco's Modified Eagle’s Medium supplemented with 10% fetal bovine serum at 37°C in humidified air with 5% CO2 and 95% air.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from cells using an RNeasy Total RNA Extraction kit (Qiagen), and treated with DNase I (Qiagen) for 15 min at room temperature to remove residual genomic DNA. RNA quality was analyzed using a Bioanalyzer 2100 (Agilent Technologies), producing RIN values between 9.5 and 10.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	500 ng of total RNA was used to synthesize cRNA with a GeneChip 3’ IVT Express Kit (Affymetrix).
Sample_hyb_protocol	After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
Sample_scan_protocol	The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
Sample_data_processing	GeneChip Analysis Suite
Sample_platform_id	GPL570
Sample_contact_name	Yoshiaki,,Tabuchi
Sample_contact_email	ytabu@cts.u-toyama.ac.jp
Sample_contact_laboratory	Division of Molecular Genetics Research
Sample_contact_department	Life Science Research Center
Sample_contact_institute	University of Toyama
Sample_contact_address	2630 Sugitani
Sample_contact_city	Toyama
Sample_contact_state	Toyama
Sample_contact_zip/postal_code	930-0194
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1068nnn/GSM1068624/suppl/GSM1068624_HeLa-0h.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1068nnn/GSM1068624/suppl/GSM1068624_HeLa-0h.CHP.gz
Sample_series_id	GSE43701
Sample_data_row_count	54675

GSM1068625

GPL570

HeLa cells-MHT 1h

Human cervical squamous cell carcinoma cell line HeLa

cell line: HeLa treatment: heat stress at 41°C and cultured for 1 h gender: female age: 31 years tissue: cervix life span: infinite morphology: epithelial-like

HeLa cells treated with heat stress at 41°C and cultured for 1 h

Sample_geo_accession	GSM1068625
Sample_status	Public on Jan 24 2013
Sample_submission_date	Jan 23 2013
Sample_last_update_date	Jan 24 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	MHT treatment was carried out by immersing plastic culture vessels containing the attached cells in a water bath at 41°C for 30 min. After heat treatment, the cells were incubated for 0-3 h at 37°C.
Sample_growth_protocol_ch1	HeLa cells were cultured in Dulbecco's Modified Eagle’s Medium supplemented with 10% fetal bovine serum at 37°C in humidified air with 5% CO2 and 95% air.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from cells using an RNeasy Total RNA Extraction kit (Qiagen), and treated with DNase I (Qiagen) for 15 min at room temperature to remove residual genomic DNA. RNA quality was analyzed using a Bioanalyzer 2100 (Agilent Technologies), producing RIN values between 9.5 and 10.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	500 ng of total RNA was used to synthesize cRNA with a GeneChip 3’ IVT Express Kit (Affymetrix).
Sample_hyb_protocol	After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
Sample_scan_protocol	The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
Sample_data_processing	GeneChip Analysis Suite
Sample_platform_id	GPL570
Sample_contact_name	Yoshiaki,,Tabuchi
Sample_contact_email	ytabu@cts.u-toyama.ac.jp
Sample_contact_laboratory	Division of Molecular Genetics Research
Sample_contact_department	Life Science Research Center
Sample_contact_institute	University of Toyama
Sample_contact_address	2630 Sugitani
Sample_contact_city	Toyama
Sample_contact_state	Toyama
Sample_contact_zip/postal_code	930-0194
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1068nnn/GSM1068625/suppl/GSM1068625_HeLa-1h.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1068nnn/GSM1068625/suppl/GSM1068625_HeLa-1h.CHP.gz
Sample_series_id	GSE43701
Sample_data_row_count	54675

GSM1068626

GPL570

HeLa cells-MHT 3h

Human cervical squamous cell carcinoma cell line HeLa

cell line: HeLa treatment: heat stress at 41°C and cultured for 3 h gender: female age: 31 years tissue: cervix life span: infinite morphology: epithelial-like

HeLa cells treated with heat stress at 41°C and cultured for 3 h

Sample_geo_accession	GSM1068626
Sample_status	Public on Jan 24 2013
Sample_submission_date	Jan 23 2013
Sample_last_update_date	Jan 24 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	MHT treatment was carried out by immersing plastic culture vessels containing the attached cells in a water bath at 41°C for 30 min. After heat treatment, the cells were incubated for 0-3 h at 37°C.
Sample_growth_protocol_ch1	HeLa cells were cultured in Dulbecco's Modified Eagle’s Medium supplemented with 10% fetal bovine serum at 37°C in humidified air with 5% CO2 and 95% air.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from cells using an RNeasy Total RNA Extraction kit (Qiagen), and treated with DNase I (Qiagen) for 15 min at room temperature to remove residual genomic DNA. RNA quality was analyzed using a Bioanalyzer 2100 (Agilent Technologies), producing RIN values between 9.5 and 10.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	500 ng of total RNA was used to synthesize cRNA with a GeneChip 3’ IVT Express Kit (Affymetrix).
Sample_hyb_protocol	After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
Sample_scan_protocol	The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
Sample_data_processing	GeneChip Analysis Suite
Sample_platform_id	GPL570
Sample_contact_name	Yoshiaki,,Tabuchi
Sample_contact_email	ytabu@cts.u-toyama.ac.jp
Sample_contact_laboratory	Division of Molecular Genetics Research
Sample_contact_department	Life Science Research Center
Sample_contact_institute	University of Toyama
Sample_contact_address	2630 Sugitani
Sample_contact_city	Toyama
Sample_contact_state	Toyama
Sample_contact_zip/postal_code	930-0194
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1068nnn/GSM1068626/suppl/GSM1068626_HeLa-3h.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1068nnn/GSM1068626/suppl/GSM1068626_HeLa-3h.CHP.gz
Sample_series_id	GSE43701
Sample_data_row_count	54675

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