Search results for the GEO ID: GSE43744 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1070148 | GPL1355 |
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Peri-implant soft tissue 1
|
Tooth extraction and implant placement
|
strain: Sprague Dawley
gender: male
treatment: Tooth extraction and implant placement
tissue: peri-implant soft tissue
|
Gene expression data from peri-implant soft tissue
|
Sample_geo_accession | GSM1070148
| Sample_status | Public on Jan 24 2013
| Sample_submission_date | Jan 24 2013
| Sample_last_update_date | Jan 24 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Both side upper first molars of rats were extracted and titanium alloy implants were placed only in the left extraction socket. Four weeks after surgery, peri-implant soft tissue from the left side and oral mucosal tissue from the site of healing after tooth extraction on the right side were harvested under a dissecting microscope.
| Sample_growth_protocol_ch1 | Four-week-old male Sprague-Dawley rats were obtained from Japan SLC Inc.( Tokyo, Japan). After surgery the animals were housed in a room with controlled temperature and humidity and a 12 h light dark cycle and given freely water and diet until sacrificed after 4 weeks.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from peri-implant soft tissue and oral mucosal tissue using an RNeasy® Mini kit (QIAGEN, Alameda, CA, USA) according to the manufacturer’s instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA were amplified, labeled and purified by using GeneChip 3’IVT Express Kit (Affymetrix, Santa Clara, CA, US) according to the manufacturer’s instructions to obtain biotin labeled aRNA.
| Sample_hyb_protocol | Following fragmentation, aRNA products (15 μg) were hybridized on GeneChip® Rat Genome 230 2.0 Array Chips (Affymetrix) for 16 h at 45°C in a GeneChip® Hybridization Oven 640 (Affymetrix).
| Sample_scan_protocol | Hybridized arrays were washed and stained by using a Gene Chip® Fluidics station 450 (Affymetrix), and the images were scanned by using a Genechip® scanner 3,000 (Affymetrix).
| Sample_data_processing | CEL files were generated using the GeneChip® Operating Software version 1.2 (Affymetrix). Data analysis and normalization were performed using GeneSpring® GX software version 12 (Agilent Technologies, Foster City, CA, USA).
| Sample_platform_id | GPL1355
| Sample_contact_name | Hodaka,,Sasaki
| Sample_contact_email | hosasaki@tdc.ac.jp
| Sample_contact_phone | +81-43-270-3573
| Sample_contact_fax | +81-43-270-3574
| Sample_contact_department | Dept. of Oral and Maxillofacial Implantology
| Sample_contact_institute | Tokyo Dental College
| Sample_contact_address | 1-2-2, Masago, Mihama-ku
| Sample_contact_city | Chiba
| Sample_contact_zip/postal_code | 261-8502
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1070nnn/GSM1070148/suppl/GSM1070148_Rat-PIST_1.CEL.gz
| Sample_series_id | GSE43744
| Sample_data_row_count | 31099
| |
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GSM1070150 | GPL1355 |
|
Peri-implant soft tissue 2
|
Tooth extraction and implant placement
|
strain: Sprague Dawley
gender: male
treatment: Tooth extraction and implant placement
tissue: peri-implant soft tissue
|
Gene expression data from peri-implant soft tissue
|
Sample_geo_accession | GSM1070150
| Sample_status | Public on Jan 24 2013
| Sample_submission_date | Jan 24 2013
| Sample_last_update_date | Jan 24 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Both side upper first molars of rats were extracted and titanium alloy implants were placed only in the left extraction socket. Four weeks after surgery, peri-implant soft tissue from the left side and oral mucosal tissue from the site of healing after tooth extraction on the right side were harvested under a dissecting microscope.
| Sample_growth_protocol_ch1 | Four-week-old male Sprague-Dawley rats were obtained from Japan SLC Inc.( Tokyo, Japan). After surgery the animals were housed in a room with controlled temperature and humidity and a 12 h light dark cycle and given freely water and diet until sacrificed after 4 weeks.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from peri-implant soft tissue and oral mucosal tissue using an RNeasy® Mini kit (QIAGEN, Alameda, CA, USA) according to the manufacturer’s instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA were amplified, labeled and purified by using GeneChip 3’IVT Express Kit (Affymetrix, Santa Clara, CA, US) according to the manufacturer’s instructions to obtain biotin labeled aRNA.
| Sample_hyb_protocol | Following fragmentation, aRNA products (15 μg) were hybridized on GeneChip® Rat Genome 230 2.0 Array Chips (Affymetrix) for 16 h at 45°C in a GeneChip® Hybridization Oven 640 (Affymetrix).
| Sample_scan_protocol | Hybridized arrays were washed and stained by using a Gene Chip® Fluidics station 450 (Affymetrix), and the images were scanned by using a Genechip® scanner 3,000 (Affymetrix).
| Sample_data_processing | CEL files were generated using the GeneChip® Operating Software version 1.2 (Affymetrix). Data analysis and normalization were performed using GeneSpring® GX software version 12 (Agilent Technologies, Foster City, CA, USA).
| Sample_platform_id | GPL1355
| Sample_contact_name | Hodaka,,Sasaki
| Sample_contact_email | hosasaki@tdc.ac.jp
| Sample_contact_phone | +81-43-270-3573
| Sample_contact_fax | +81-43-270-3574
| Sample_contact_department | Dept. of Oral and Maxillofacial Implantology
| Sample_contact_institute | Tokyo Dental College
| Sample_contact_address | 1-2-2, Masago, Mihama-ku
| Sample_contact_city | Chiba
| Sample_contact_zip/postal_code | 261-8502
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1070nnn/GSM1070150/suppl/GSM1070150_Rat-PIST_2.CEL.gz
| Sample_series_id | GSE43744
| Sample_data_row_count | 31099
| |
|
GSM1070152 | GPL1355 |
|
Peri-implant soft tissue 3
|
Tooth extraction and implant placement
|
strain: Sprague Dawley
gender: male
treatment: Tooth extraction and implant placement
tissue: peri-implant soft tissue
|
Gene expression data from peri-implant soft tissue
|
Sample_geo_accession | GSM1070152
| Sample_status | Public on Jan 24 2013
| Sample_submission_date | Jan 24 2013
| Sample_last_update_date | Jan 24 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Both side upper first molars of rats were extracted and titanium alloy implants were placed only in the left extraction socket. Four weeks after surgery, peri-implant soft tissue from the left side and oral mucosal tissue from the site of healing after tooth extraction on the right side were harvested under a dissecting microscope.
| Sample_growth_protocol_ch1 | Four-week-old male Sprague-Dawley rats were obtained from Japan SLC Inc.( Tokyo, Japan). After surgery the animals were housed in a room with controlled temperature and humidity and a 12 h light dark cycle and given freely water and diet until sacrificed after 4 weeks.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from peri-implant soft tissue and oral mucosal tissue using an RNeasy® Mini kit (QIAGEN, Alameda, CA, USA) according to the manufacturer’s instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA were amplified, labeled and purified by using GeneChip 3’IVT Express Kit (Affymetrix, Santa Clara, CA, US) according to the manufacturer’s instructions to obtain biotin labeled aRNA.
| Sample_hyb_protocol | Following fragmentation, aRNA products (15 μg) were hybridized on GeneChip® Rat Genome 230 2.0 Array Chips (Affymetrix) for 16 h at 45°C in a GeneChip® Hybridization Oven 640 (Affymetrix).
| Sample_scan_protocol | Hybridized arrays were washed and stained by using a Gene Chip® Fluidics station 450 (Affymetrix), and the images were scanned by using a Genechip® scanner 3,000 (Affymetrix).
| Sample_data_processing | CEL files were generated using the GeneChip® Operating Software version 1.2 (Affymetrix). Data analysis and normalization were performed using GeneSpring® GX software version 12 (Agilent Technologies, Foster City, CA, USA).
| Sample_platform_id | GPL1355
| Sample_contact_name | Hodaka,,Sasaki
| Sample_contact_email | hosasaki@tdc.ac.jp
| Sample_contact_phone | +81-43-270-3573
| Sample_contact_fax | +81-43-270-3574
| Sample_contact_department | Dept. of Oral and Maxillofacial Implantology
| Sample_contact_institute | Tokyo Dental College
| Sample_contact_address | 1-2-2, Masago, Mihama-ku
| Sample_contact_city | Chiba
| Sample_contact_zip/postal_code | 261-8502
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1070nnn/GSM1070152/suppl/GSM1070152_Rat-PIST_3.CEL.gz
| Sample_series_id | GSE43744
| Sample_data_row_count | 31099
| |
|
GSM1070153 | GPL1355 |
|
Oral mucosal tissue 1
|
Tooth extraction only
|
strain: Sprague Dawley
gender: male
treatment: Tooth extraction only
tissue: oral mucosal tissue
|
Gene expression data from oral mucosal tissue
|
Sample_geo_accession | GSM1070153
| Sample_status | Public on Jan 24 2013
| Sample_submission_date | Jan 24 2013
| Sample_last_update_date | Jan 24 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Both side upper first molars of rats were extracted and titanium alloy implants were placed only in the left extraction socket. Four weeks after surgery, peri-implant soft tissue from the left side and oral mucosal tissue from the site of healing after tooth extraction on the right side were harvested under a dissecting microscope.
| Sample_growth_protocol_ch1 | Four-week-old male Sprague-Dawley rats were obtained from Japan SLC Inc.( Tokyo, Japan). After surgery the animals were housed in a room with controlled temperature and humidity and a 12 h light dark cycle and given freely water and diet until sacrificed after 4 weeks.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from peri-implant soft tissue and oral mucosal tissue using an RNeasy® Mini kit (QIAGEN, Alameda, CA, USA) according to the manufacturer’s instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA were amplified, labeled and purified by using GeneChip 3’IVT Express Kit (Affymetrix, Santa Clara, CA, US) according to the manufacturer’s instructions to obtain biotin labeled aRNA.
| Sample_hyb_protocol | Following fragmentation, aRNA products (15 μg) were hybridized on GeneChip® Rat Genome 230 2.0 Array Chips (Affymetrix) for 16 h at 45°C in a GeneChip® Hybridization Oven 640 (Affymetrix).
| Sample_scan_protocol | Hybridized arrays were washed and stained by using a Gene Chip® Fluidics station 450 (Affymetrix), and the images were scanned by using a Genechip® scanner 3,000 (Affymetrix).
| Sample_data_processing | CEL files were generated using the GeneChip® Operating Software version 1.2 (Affymetrix). Data analysis and normalization were performed using GeneSpring® GX software version 12 (Agilent Technologies, Foster City, CA, USA).
| Sample_platform_id | GPL1355
| Sample_contact_name | Hodaka,,Sasaki
| Sample_contact_email | hosasaki@tdc.ac.jp
| Sample_contact_phone | +81-43-270-3573
| Sample_contact_fax | +81-43-270-3574
| Sample_contact_department | Dept. of Oral and Maxillofacial Implantology
| Sample_contact_institute | Tokyo Dental College
| Sample_contact_address | 1-2-2, Masago, Mihama-ku
| Sample_contact_city | Chiba
| Sample_contact_zip/postal_code | 261-8502
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1070nnn/GSM1070153/suppl/GSM1070153_Rat-OMT_1.CEL.gz
| Sample_series_id | GSE43744
| Sample_data_row_count | 31099
| |
|
GSM1070155 | GPL1355 |
|
Oral mucosal tissue 2
|
Tooth extraction only
|
strain: Sprague Dawley
gender: male
treatment: Tooth extraction only
tissue: oral mucosal tissue
|
Gene expression data from oral mucosal tissue
|
Sample_geo_accession | GSM1070155
| Sample_status | Public on Jan 24 2013
| Sample_submission_date | Jan 24 2013
| Sample_last_update_date | Jan 24 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Both side upper first molars of rats were extracted and titanium alloy implants were placed only in the left extraction socket. Four weeks after surgery, peri-implant soft tissue from the left side and oral mucosal tissue from the site of healing after tooth extraction on the right side were harvested under a dissecting microscope.
| Sample_growth_protocol_ch1 | Four-week-old male Sprague-Dawley rats were obtained from Japan SLC Inc.( Tokyo, Japan). After surgery the animals were housed in a room with controlled temperature and humidity and a 12 h light dark cycle and given freely water and diet until sacrificed after 4 weeks.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from peri-implant soft tissue and oral mucosal tissue using an RNeasy® Mini kit (QIAGEN, Alameda, CA, USA) according to the manufacturer’s instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA were amplified, labeled and purified by using GeneChip 3’IVT Express Kit (Affymetrix, Santa Clara, CA, US) according to the manufacturer’s instructions to obtain biotin labeled aRNA.
| Sample_hyb_protocol | Following fragmentation, aRNA products (15 μg) were hybridized on GeneChip® Rat Genome 230 2.0 Array Chips (Affymetrix) for 16 h at 45°C in a GeneChip® Hybridization Oven 640 (Affymetrix).
| Sample_scan_protocol | Hybridized arrays were washed and stained by using a Gene Chip® Fluidics station 450 (Affymetrix), and the images were scanned by using a Genechip® scanner 3,000 (Affymetrix).
| Sample_data_processing | CEL files were generated using the GeneChip® Operating Software version 1.2 (Affymetrix). Data analysis and normalization were performed using GeneSpring® GX software version 12 (Agilent Technologies, Foster City, CA, USA).
| Sample_platform_id | GPL1355
| Sample_contact_name | Hodaka,,Sasaki
| Sample_contact_email | hosasaki@tdc.ac.jp
| Sample_contact_phone | +81-43-270-3573
| Sample_contact_fax | +81-43-270-3574
| Sample_contact_department | Dept. of Oral and Maxillofacial Implantology
| Sample_contact_institute | Tokyo Dental College
| Sample_contact_address | 1-2-2, Masago, Mihama-ku
| Sample_contact_city | Chiba
| Sample_contact_zip/postal_code | 261-8502
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1070nnn/GSM1070155/suppl/GSM1070155_Rat-OMT_2.CEL.gz
| Sample_series_id | GSE43744
| Sample_data_row_count | 31099
| |
|
GSM1070156 | GPL1355 |
|
Oral mucosal tissue 3
|
Tooth extraction only
|
strain: Sprague Dawley
gender: male
treatment: Tooth extraction only
tissue: oral mucosal tissue
|
Gene expression data from oral mucosal tissue
|
Sample_geo_accession | GSM1070156
| Sample_status | Public on Jan 24 2013
| Sample_submission_date | Jan 24 2013
| Sample_last_update_date | Jan 24 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Both side upper first molars of rats were extracted and titanium alloy implants were placed only in the left extraction socket. Four weeks after surgery, peri-implant soft tissue from the left side and oral mucosal tissue from the site of healing after tooth extraction on the right side were harvested under a dissecting microscope.
| Sample_growth_protocol_ch1 | Four-week-old male Sprague-Dawley rats were obtained from Japan SLC Inc.( Tokyo, Japan). After surgery the animals were housed in a room with controlled temperature and humidity and a 12 h light dark cycle and given freely water and diet until sacrificed after 4 weeks.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from peri-implant soft tissue and oral mucosal tissue using an RNeasy® Mini kit (QIAGEN, Alameda, CA, USA) according to the manufacturer’s instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA were amplified, labeled and purified by using GeneChip 3’IVT Express Kit (Affymetrix, Santa Clara, CA, US) according to the manufacturer’s instructions to obtain biotin labeled aRNA.
| Sample_hyb_protocol | Following fragmentation, aRNA products (15 μg) were hybridized on GeneChip® Rat Genome 230 2.0 Array Chips (Affymetrix) for 16 h at 45°C in a GeneChip® Hybridization Oven 640 (Affymetrix).
| Sample_scan_protocol | Hybridized arrays were washed and stained by using a Gene Chip® Fluidics station 450 (Affymetrix), and the images were scanned by using a Genechip® scanner 3,000 (Affymetrix).
| Sample_data_processing | CEL files were generated using the GeneChip® Operating Software version 1.2 (Affymetrix). Data analysis and normalization were performed using GeneSpring® GX software version 12 (Agilent Technologies, Foster City, CA, USA).
| Sample_platform_id | GPL1355
| Sample_contact_name | Hodaka,,Sasaki
| Sample_contact_email | hosasaki@tdc.ac.jp
| Sample_contact_phone | +81-43-270-3573
| Sample_contact_fax | +81-43-270-3574
| Sample_contact_department | Dept. of Oral and Maxillofacial Implantology
| Sample_contact_institute | Tokyo Dental College
| Sample_contact_address | 1-2-2, Masago, Mihama-ku
| Sample_contact_city | Chiba
| Sample_contact_zip/postal_code | 261-8502
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1070nnn/GSM1070156/suppl/GSM1070156_Rat-OMT_3.CEL.gz
| Sample_series_id | GSE43744
| Sample_data_row_count | 31099
| |
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