Result

Search results for the GEO ID: GSE43762

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM1070468

GPL570

GSC03A_Sphere_2day

GIC03A cultured for 2 days without FCS

cell: Glioma initiating cell

Sample_geo_accession	GSM1070468
Sample_status	Public on Jan 26 2013
Sample_submission_date	Jan 25 2013
Sample_last_update_date	Jan 26 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The GICs were cultured in NSC medium with (differentiation condition) or without (sphere condition) 10% FCS for 2 days and 7 days after the sphere dispersion by Accumax (Innovative Cell Technologies).
Sample_growth_protocol_ch1	The GIC clones prepared from human glioma tissues were cultured in NSC medium; Neurobasal-A Medium (GIBCO/Invitrogen), B-27 (1:50; GIBCO/Invitrogen), heparin (5 g/ml; SIGMA), and GlutaMax-1 (GIBCO) containing recombinant hFGF-2 (20 ng/ml; PeproTech Inc), recombinant hEGF (20 ng/ml; PeproTech Inc), recombinant hLIF (20 ng/ml; Chemicon Inc), and insulin (10 ng/ml, SIGMA)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Extraction of total RNA was performed with Quiagen RNAeasy according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 100 ng of total RNA according to the standard Affymetrix protocol (GeneChip Expression Analysis Technical Manual, Affymetrix, P/N 702232 Rev. 3).
Sample_hyb_protocol	After the labeled cRNA purification and fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45oC on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array in the Affymetrix GeneChip Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	norie,,araki
Sample_contact_email	nori@gpo.kumamoto-u.ac.jp
Sample_contact_department	tumor genetics and biology
Sample_contact_institute	kumamoto university
Sample_contact_address	1-1-1, honjo
Sample_contact_city	kumamoto
Sample_contact_zip/postal_code	860-8556
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1070nnn/GSM1070468/suppl/GSM1070468_GSC03A_Sphere_2day_HG-U133_Plus_2_2.CEL.gz
Sample_series_id	GSE43762
Sample_data_row_count	54675

GSM1070469

GPL570

GSC03U_Sphere_2day

GIC03U cultured for 2 days without FCS

cell: Glioma initiating cell

Sample_geo_accession	GSM1070469
Sample_status	Public on Jan 26 2013
Sample_submission_date	Jan 25 2013
Sample_last_update_date	Jan 26 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The GICs were cultured in NSC medium with (differentiation condition) or without (sphere condition) 10% FCS for 2 days and 7 days after the sphere dispersion by Accumax (Innovative Cell Technologies).
Sample_growth_protocol_ch1	The GIC clones prepared from human glioma tissues were cultured in NSC medium; Neurobasal-A Medium (GIBCO/Invitrogen), B-27 (1:50; GIBCO/Invitrogen), heparin (5 g/ml; SIGMA), and GlutaMax-1 (GIBCO) containing recombinant hFGF-2 (20 ng/ml; PeproTech Inc), recombinant hEGF (20 ng/ml; PeproTech Inc), recombinant hLIF (20 ng/ml; Chemicon Inc), and insulin (10 ng/ml, SIGMA)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Extraction of total RNA was performed with Quiagen RNAeasy according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 100 ng of total RNA according to the standard Affymetrix protocol (GeneChip Expression Analysis Technical Manual, Affymetrix, P/N 702232 Rev. 3).
Sample_hyb_protocol	After the labeled cRNA purification and fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45oC on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array in the Affymetrix GeneChip Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	norie,,araki
Sample_contact_email	nori@gpo.kumamoto-u.ac.jp
Sample_contact_department	tumor genetics and biology
Sample_contact_institute	kumamoto university
Sample_contact_address	1-1-1, honjo
Sample_contact_city	kumamoto
Sample_contact_zip/postal_code	860-8556
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1070nnn/GSM1070469/suppl/GSM1070469_GSC03U_Sphere_2day_HG-U133_Plus_2_.CEL.gz
Sample_series_id	GSE43762
Sample_data_row_count	54675

GSM1070470

GPL570

GSC03A_Sphere_7day

GIC03A cultured for 7 days without FCS

cell: Glioma initiating cell

Sample_geo_accession	GSM1070470
Sample_status	Public on Jan 26 2013
Sample_submission_date	Jan 25 2013
Sample_last_update_date	Jan 26 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The GICs were cultured in NSC medium with (differentiation condition) or without (sphere condition) 10% FCS for 2 days and 7 days after the sphere dispersion by Accumax (Innovative Cell Technologies).
Sample_growth_protocol_ch1	The GIC clones prepared from human glioma tissues were cultured in NSC medium; Neurobasal-A Medium (GIBCO/Invitrogen), B-27 (1:50; GIBCO/Invitrogen), heparin (5 g/ml; SIGMA), and GlutaMax-1 (GIBCO) containing recombinant hFGF-2 (20 ng/ml; PeproTech Inc), recombinant hEGF (20 ng/ml; PeproTech Inc), recombinant hLIF (20 ng/ml; Chemicon Inc), and insulin (10 ng/ml, SIGMA)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Extraction of total RNA was performed with Quiagen RNAeasy according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 100 ng of total RNA according to the standard Affymetrix protocol (GeneChip Expression Analysis Technical Manual, Affymetrix, P/N 702232 Rev. 3).
Sample_hyb_protocol	After the labeled cRNA purification and fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45oC on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array in the Affymetrix GeneChip Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	norie,,araki
Sample_contact_email	nori@gpo.kumamoto-u.ac.jp
Sample_contact_department	tumor genetics and biology
Sample_contact_institute	kumamoto university
Sample_contact_address	1-1-1, honjo
Sample_contact_city	kumamoto
Sample_contact_zip/postal_code	860-8556
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1070nnn/GSM1070470/suppl/GSM1070470_GSC03A_Sphere_7day_HG-U133_Plus_2_.CEL.gz
Sample_series_id	GSE43762
Sample_data_row_count	54675

GSM1070471

GPL570

GSC03U_Sphere_7day

GIC03U cultured for 7 days without FCS

cell: Glioma initiating cell

Sample_geo_accession	GSM1070471
Sample_status	Public on Jan 26 2013
Sample_submission_date	Jan 25 2013
Sample_last_update_date	Jan 26 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The GICs were cultured in NSC medium with (differentiation condition) or without (sphere condition) 10% FCS for 2 days and 7 days after the sphere dispersion by Accumax (Innovative Cell Technologies).
Sample_growth_protocol_ch1	The GIC clones prepared from human glioma tissues were cultured in NSC medium; Neurobasal-A Medium (GIBCO/Invitrogen), B-27 (1:50; GIBCO/Invitrogen), heparin (5 g/ml; SIGMA), and GlutaMax-1 (GIBCO) containing recombinant hFGF-2 (20 ng/ml; PeproTech Inc), recombinant hEGF (20 ng/ml; PeproTech Inc), recombinant hLIF (20 ng/ml; Chemicon Inc), and insulin (10 ng/ml, SIGMA)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Extraction of total RNA was performed with Quiagen RNAeasy according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 100 ng of total RNA according to the standard Affymetrix protocol (GeneChip Expression Analysis Technical Manual, Affymetrix, P/N 702232 Rev. 3).
Sample_hyb_protocol	After the labeled cRNA purification and fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45oC on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array in the Affymetrix GeneChip Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	norie,,araki
Sample_contact_email	nori@gpo.kumamoto-u.ac.jp
Sample_contact_department	tumor genetics and biology
Sample_contact_institute	kumamoto university
Sample_contact_address	1-1-1, honjo
Sample_contact_city	kumamoto
Sample_contact_zip/postal_code	860-8556
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1070nnn/GSM1070471/suppl/GSM1070471_GSC03U_Sphere_7day_HG-U133_Plus_2_.CEL.gz
Sample_series_id	GSE43762
Sample_data_row_count	54675

GSM1070472

GPL570

GSC03A_Differentiation_2day

GIC03A cultured for 2 days with FCS

cell: Glioma initiating cell

Sample_geo_accession	GSM1070472
Sample_status	Public on Jan 26 2013
Sample_submission_date	Jan 25 2013
Sample_last_update_date	Jan 26 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The GICs were cultured in NSC medium with (differentiation condition) or without (sphere condition) 10% FCS for 2 days and 7 days after the sphere dispersion by Accumax (Innovative Cell Technologies).
Sample_growth_protocol_ch1	The GIC clones prepared from human glioma tissues were cultured in NSC medium; Neurobasal-A Medium (GIBCO/Invitrogen), B-27 (1:50; GIBCO/Invitrogen), heparin (5 g/ml; SIGMA), and GlutaMax-1 (GIBCO) containing recombinant hFGF-2 (20 ng/ml; PeproTech Inc), recombinant hEGF (20 ng/ml; PeproTech Inc), recombinant hLIF (20 ng/ml; Chemicon Inc), and insulin (10 ng/ml, SIGMA)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Extraction of total RNA was performed with Quiagen RNAeasy according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 100 ng of total RNA according to the standard Affymetrix protocol (GeneChip Expression Analysis Technical Manual, Affymetrix, P/N 702232 Rev. 3).
Sample_hyb_protocol	After the labeled cRNA purification and fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45oC on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array in the Affymetrix GeneChip Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	norie,,araki
Sample_contact_email	nori@gpo.kumamoto-u.ac.jp
Sample_contact_department	tumor genetics and biology
Sample_contact_institute	kumamoto university
Sample_contact_address	1-1-1, honjo
Sample_contact_city	kumamoto
Sample_contact_zip/postal_code	860-8556
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1070nnn/GSM1070472/suppl/GSM1070472_GSC03A_Differentiation_2day-3_HG-U133_Plus_2_.CEL.gz
Sample_series_id	GSE43762
Sample_data_row_count	54675

GSM1070473

GPL570

GSC03U_Differentiation_2day

GIC03U cultured for 2 days with FCS

cell: Glioma initiating cell

Sample_geo_accession	GSM1070473
Sample_status	Public on Jan 26 2013
Sample_submission_date	Jan 25 2013
Sample_last_update_date	Jan 26 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The GICs were cultured in NSC medium with (differentiation condition) or without (sphere condition) 10% FCS for 2 days and 7 days after the sphere dispersion by Accumax (Innovative Cell Technologies).
Sample_growth_protocol_ch1	The GIC clones prepared from human glioma tissues were cultured in NSC medium; Neurobasal-A Medium (GIBCO/Invitrogen), B-27 (1:50; GIBCO/Invitrogen), heparin (5 g/ml; SIGMA), and GlutaMax-1 (GIBCO) containing recombinant hFGF-2 (20 ng/ml; PeproTech Inc), recombinant hEGF (20 ng/ml; PeproTech Inc), recombinant hLIF (20 ng/ml; Chemicon Inc), and insulin (10 ng/ml, SIGMA)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Extraction of total RNA was performed with Quiagen RNAeasy according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 100 ng of total RNA according to the standard Affymetrix protocol (GeneChip Expression Analysis Technical Manual, Affymetrix, P/N 702232 Rev. 3).
Sample_hyb_protocol	After the labeled cRNA purification and fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45oC on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array in the Affymetrix GeneChip Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	norie,,araki
Sample_contact_email	nori@gpo.kumamoto-u.ac.jp
Sample_contact_department	tumor genetics and biology
Sample_contact_institute	kumamoto university
Sample_contact_address	1-1-1, honjo
Sample_contact_city	kumamoto
Sample_contact_zip/postal_code	860-8556
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1070nnn/GSM1070473/suppl/GSM1070473_GSC03U_Differentiation_2day-3_HG-U133_Plus_2_.CEL.gz
Sample_series_id	GSE43762
Sample_data_row_count	54675

GSM1070474

GPL570

GSC03A_Differentiation_7day

GIC03A cultured for 7 days with FCS

cell: Glioma initiating cell

Sample_geo_accession	GSM1070474
Sample_status	Public on Jan 26 2013
Sample_submission_date	Jan 25 2013
Sample_last_update_date	Jan 26 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The GICs were cultured in NSC medium with (differentiation condition) or without (sphere condition) 10% FCS for 2 days and 7 days after the sphere dispersion by Accumax (Innovative Cell Technologies).
Sample_growth_protocol_ch1	The GIC clones prepared from human glioma tissues were cultured in NSC medium; Neurobasal-A Medium (GIBCO/Invitrogen), B-27 (1:50; GIBCO/Invitrogen), heparin (5 g/ml; SIGMA), and GlutaMax-1 (GIBCO) containing recombinant hFGF-2 (20 ng/ml; PeproTech Inc), recombinant hEGF (20 ng/ml; PeproTech Inc), recombinant hLIF (20 ng/ml; Chemicon Inc), and insulin (10 ng/ml, SIGMA)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Extraction of total RNA was performed with Quiagen RNAeasy according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 100 ng of total RNA according to the standard Affymetrix protocol (GeneChip Expression Analysis Technical Manual, Affymetrix, P/N 702232 Rev. 3).
Sample_hyb_protocol	After the labeled cRNA purification and fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45oC on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array in the Affymetrix GeneChip Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	norie,,araki
Sample_contact_email	nori@gpo.kumamoto-u.ac.jp
Sample_contact_department	tumor genetics and biology
Sample_contact_institute	kumamoto university
Sample_contact_address	1-1-1, honjo
Sample_contact_city	kumamoto
Sample_contact_zip/postal_code	860-8556
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1070nnn/GSM1070474/suppl/GSM1070474_GSC03A_Differentiation_7day_HG-U133_Plus_2_.CEL.gz
Sample_series_id	GSE43762
Sample_data_row_count	54675

GSM1070475

GPL570

GSC03U_Differentiation_7day

GIC03U cultured for 7 days with FCS

cell: Glioma initiating cell

Sample_geo_accession	GSM1070475
Sample_status	Public on Jan 26 2013
Sample_submission_date	Jan 25 2013
Sample_last_update_date	Jan 26 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The GICs were cultured in NSC medium with (differentiation condition) or without (sphere condition) 10% FCS for 2 days and 7 days after the sphere dispersion by Accumax (Innovative Cell Technologies).
Sample_growth_protocol_ch1	The GIC clones prepared from human glioma tissues were cultured in NSC medium; Neurobasal-A Medium (GIBCO/Invitrogen), B-27 (1:50; GIBCO/Invitrogen), heparin (5 g/ml; SIGMA), and GlutaMax-1 (GIBCO) containing recombinant hFGF-2 (20 ng/ml; PeproTech Inc), recombinant hEGF (20 ng/ml; PeproTech Inc), recombinant hLIF (20 ng/ml; Chemicon Inc), and insulin (10 ng/ml, SIGMA)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Extraction of total RNA was performed with Quiagen RNAeasy according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 100 ng of total RNA according to the standard Affymetrix protocol (GeneChip Expression Analysis Technical Manual, Affymetrix, P/N 702232 Rev. 3).
Sample_hyb_protocol	After the labeled cRNA purification and fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45oC on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array in the Affymetrix GeneChip Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	norie,,araki
Sample_contact_email	nori@gpo.kumamoto-u.ac.jp
Sample_contact_department	tumor genetics and biology
Sample_contact_institute	kumamoto university
Sample_contact_address	1-1-1, honjo
Sample_contact_city	kumamoto
Sample_contact_zip/postal_code	860-8556
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1070nnn/GSM1070475/suppl/GSM1070475_GSC03U_Differentiation_7day_HG-U133_Plus_2_.CEL.gz
Sample_series_id	GSE43762
Sample_data_row_count	54675

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