Search results for the GEO ID: GSE43814  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1071644 | GPL1261 |
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Platelets from Smad4 f/f mice
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Platelet
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cell type: platelets
strain: C57BL/6
genotype: Smad4 f/f
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Gene expression in platelets from wildtype mice, control
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| Sample_geo_accession | GSM1071644
| | Sample_status | Public on Jan 29 2013
| | Sample_submission_date | Jan 28 2013
| | Sample_last_update_date | Jan 29 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were genotyped by PCR, and Smad4 deficiency in platelets was confirmed by Western blotting.
| | Sample_growth_protocol_ch1 | Mice carrying Smad4 gene flanked by loxP recognition sites (Smad4f/f) 7 were crossed with transgenic mice carrying Cre recombinase under the control of the PF4 promoter (PF4-Cre) 8 to generate mice with conditional Magakaryocyte/platelet specific Smad4 deficiency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using mirVanaTM PARISTM (Cat#AM1561, Ambion, Austin, TX, US) following the manufacturer’s instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA were amplified, labeled and purified by using GeneChip 3’IVT Express Kit (Cat#901229, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions to obtain biotin labeled cRNA.
| | Sample_hyb_protocol | Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00-0079, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.
| | Sample_scan_protocol | Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software 3.1 (Affymetrix, Santa Clara, CA, US) with default settings.
| | Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Junling,,Liu
| | Sample_contact_email | liujl@shsmu.edu.cn
| | Sample_contact_institute | Shanghai Jiaotong University
| | Sample_contact_address | No.280 Chongqing Road
| | Sample_contact_city | Shanghai
| | Sample_contact_zip/postal_code | 200025
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1071nnn/GSM1071644/suppl/GSM1071644_BH12514-3_WT_PLT_Mouse430_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1071nnn/GSM1071644/suppl/GSM1071644_BH12514-3_WT_PLT_Mouse430_2_.mas5.CHP.gz
| | Sample_series_id | GSE43814
| | Sample_data_row_count | 45101
| | |
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GSM1071645 | GPL1261 |
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Platelets from Smad4 −/− mice
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Platelet
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cell type: platelets
strain: C57BL/6
genotype: Smad4 −/−
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Gene expression in platelets from Smad4 knock out mice
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| Sample_geo_accession | GSM1071645
| | Sample_status | Public on Jan 29 2013
| | Sample_submission_date | Jan 28 2013
| | Sample_last_update_date | Jan 29 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were genotyped by PCR, and Smad4 deficiency in platelets was confirmed by Western blotting.
| | Sample_growth_protocol_ch1 | Mice carrying Smad4 gene flanked by loxP recognition sites (Smad4f/f) 7 were crossed with transgenic mice carrying Cre recombinase under the control of the PF4 promoter (PF4-Cre) 8 to generate mice with conditional Magakaryocyte/platelet specific Smad4 deficiency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using mirVanaTM PARISTM (Cat#AM1561, Ambion, Austin, TX, US) following the manufacturer’s instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA were amplified, labeled and purified by using GeneChip 3’IVT Express Kit (Cat#901229, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions to obtain biotin labeled cRNA.
| | Sample_hyb_protocol | Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00-0079, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.
| | Sample_scan_protocol | Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software 3.1 (Affymetrix, Santa Clara, CA, US) with default settings.
| | Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Junling,,Liu
| | Sample_contact_email | liujl@shsmu.edu.cn
| | Sample_contact_institute | Shanghai Jiaotong University
| | Sample_contact_address | No.280 Chongqing Road
| | Sample_contact_city | Shanghai
| | Sample_contact_zip/postal_code | 200025
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1071nnn/GSM1071645/suppl/GSM1071645_BH12514-3_KO_PLT_Mouse430_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1071nnn/GSM1071645/suppl/GSM1071645_BH12514-3_KO_PLT_Mouse430_2_.mas5.CHP.gz
| | Sample_series_id | GSE43814
| | Sample_data_row_count | 45101
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