Sample_geo_accession | GSM1071842
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Sample_status | Public on Feb 02 2013
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Sample_submission_date | Jan 28 2013
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Sample_last_update_date | Feb 02 2013
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Sample_type | RNA
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Sample_channel_count | 1
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Sample_organism_ch1 | Mus musculus
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Sample_taxid_ch1 | 10090
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Sample_treatment_protocol_ch1 | The iGata5 ESC line was used to generate embryoid bodies, which were induced with doxycycline (1ug/ml) at day 4 for 48 hr, after which the embryoid bodies were plated for differentiation.
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Sample_growth_protocol_ch1 | rtTA-expressing ES cells Aniv18 were used for derivation and validation line that allow conditional expression of Gata5 and were maintained without feeders on gelatin in serum-free conditions (2% LIF, 3 mM CHIR 99021 (STEMGENT), 1 mM PD0325901 (STEMGENT),and doxycycline (1ug/ml))
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Sample_molecule_ch1 | total RNA
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Sample_extract_protocol_ch1 | Total RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit.
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Sample_label_ch1 | biotin
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Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
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Sample_hyb_protocol | Following fragmentation, cRNA were hybridized on Affymetrix Mouse Genome 430 Plus 2.0 GeneChip arrays (Santa Clara, CA).
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Sample_scan_protocol | Arrays were scanned using GeneChip Scanner 3000 7G.
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Sample_data_processing | Affymetrix GeneChip Operating Software was used for image acquisition. The data normalization and statistical analysis were performed with Array Star 2 (DNASTAR, Inc,Madison, WI).
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Sample_platform_id | GPL1261
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Sample_contact_name | Todd,,Evans
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Sample_contact_email | tre2003@med.cornell.edu
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Sample_contact_institute | Weill Medical College of Cornell University
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Sample_contact_address | 1300 York Avenue Box 282
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Sample_contact_city | New York
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Sample_contact_state | New York
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Sample_contact_zip/postal_code | 10065
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Sample_contact_country | USA
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Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1071nnn/GSM1071842/suppl/GSM1071842_iGata5.CEL.gz
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Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1071nnn/GSM1071842/suppl/GSM1071842_iGata5.CHP.gz
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Sample_series_id | GSE43831
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Sample_data_row_count | 45037
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