Search results for the GEO ID: GSE43862  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1072426 | GPL570 |
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HSC-3 cells-control
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Human oral squamous cell carcinoma cell line HSC-3, control
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cell line: HSC-3
cell type: oral squamous cell carcinoma cells
morphology: epithelial-like
life span: infinite
treatment: none
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HSC-3-C
HSC-3 cells (control).
HSC-3 cells are derived from the tongue of a 64-year-old male.
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| Sample_geo_accession | GSM1072426
| | Sample_status | Public on Jan 30 2013
| | Sample_submission_date | Jan 29 2013
| | Sample_last_update_date | Jan 30 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MHT treatment was carried out by immersing plastic culture vessels containing the attached cells in a water bath at 41°C for 30 min. After heat treatment, the cells were incubated for 0-3 h at 37°C.
| | Sample_growth_protocol_ch1 | HSC-3 cells were cultured in Eagle's Minimum Essential Medium supplemented with 10% fetal bovine serum at 37°C in humidified air with 5% CO2 and 95% air.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells using an RNeasy Total RNA Extraction kit (Qiagen), and treated with DNase I (Qiagen) for 15 min at room temperature to remove residual genomic DNA. RNA quality was analyzed using a Bioanalyzer 2100 (Agilent Technologies), producing RIN values between 9.5 and 10.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 500 ng of total RNA was used to synthesize cRNA with a GeneChip 3' IVT Express Kit (Affymetrix).
| | Sample_hyb_protocol | After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
| | Sample_scan_protocol | The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
| | Sample_data_processing | Data were processed using RMA using GeneChip Analysis Suite.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yoshiaki,,Tabuchi
| | Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| | Sample_contact_laboratory | Division of Molecular Genetics Research
| | Sample_contact_department | Life Science Research Center
| | Sample_contact_institute | University of Toyama
| | Sample_contact_address | 2630 Sugitani
| | Sample_contact_city | Toyama
| | Sample_contact_state | Toyama
| | Sample_contact_zip/postal_code | 930-0194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1072nnn/GSM1072426/suppl/GSM1072426_HSC-3-C.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1072nnn/GSM1072426/suppl/GSM1072426_HSC-3-C.CHP.gz
| | Sample_series_id | GSE43862
| | Sample_data_row_count | 54675
| | |
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GSM1072427 | GPL570 |
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HSC-3 cells-MHT 0h
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Human oral squamous cell carcinoma cell line HSC-3, MHT, 0h
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cell line: HSC-3
cell type: oral squamous cell carcinoma cells
morphology: epithelial-like
life span: infinite
treatment: heat stress
time point: 0 hr
|
HSC-3-0h
HSC-3 cells treated with heat stress at 41°C and cultured for 0 h.
HSC-3 cells are derived from the tongue of a 64-year-old male.
|
| Sample_geo_accession | GSM1072427
| | Sample_status | Public on Jan 30 2013
| | Sample_submission_date | Jan 29 2013
| | Sample_last_update_date | Jan 30 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MHT treatment was carried out by immersing plastic culture vessels containing the attached cells in a water bath at 41°C for 30 min. After heat treatment, the cells were incubated for 0-3 h at 37°C.
| | Sample_growth_protocol_ch1 | HSC-3 cells were cultured in Eagle's Minimum Essential Medium supplemented with 10% fetal bovine serum at 37°C in humidified air with 5% CO2 and 95% air.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells using an RNeasy Total RNA Extraction kit (Qiagen), and treated with DNase I (Qiagen) for 15 min at room temperature to remove residual genomic DNA. RNA quality was analyzed using a Bioanalyzer 2100 (Agilent Technologies), producing RIN values between 9.5 and 10.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 500 ng of total RNA was used to synthesize cRNA with a GeneChip 3' IVT Express Kit (Affymetrix).
| | Sample_hyb_protocol | After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
| | Sample_scan_protocol | The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
| | Sample_data_processing | Data were processed using RMA using GeneChip Analysis Suite.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yoshiaki,,Tabuchi
| | Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| | Sample_contact_laboratory | Division of Molecular Genetics Research
| | Sample_contact_department | Life Science Research Center
| | Sample_contact_institute | University of Toyama
| | Sample_contact_address | 2630 Sugitani
| | Sample_contact_city | Toyama
| | Sample_contact_state | Toyama
| | Sample_contact_zip/postal_code | 930-0194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1072nnn/GSM1072427/suppl/GSM1072427_HSC-3-0h.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1072nnn/GSM1072427/suppl/GSM1072427_HSC-3-0h.CHP.gz
| | Sample_series_id | GSE43862
| | Sample_data_row_count | 54675
| | |
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GSM1072428 | GPL570 |
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HSC-3 cells-MHT 1h
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Human oral squamous cell carcinoma cell line HSC-3, MHT, 1h
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cell line: HSC-3
cell type: oral squamous cell carcinoma cells
morphology: epithelial-like
life span: infinite
treatment: heat stress
time point: 1 hr
|
HSC-3-1h
HSC-3 cells treated with heat stress at 41°C and cultured for 1 h.
HSC-3 cells are derived from the tongue of a 64-year-old male.
|
| Sample_geo_accession | GSM1072428
| | Sample_status | Public on Jan 30 2013
| | Sample_submission_date | Jan 29 2013
| | Sample_last_update_date | Jan 30 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MHT treatment was carried out by immersing plastic culture vessels containing the attached cells in a water bath at 41°C for 30 min. After heat treatment, the cells were incubated for 0-3 h at 37°C.
| | Sample_growth_protocol_ch1 | HSC-3 cells were cultured in Eagle's Minimum Essential Medium supplemented with 10% fetal bovine serum at 37°C in humidified air with 5% CO2 and 95% air.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells using an RNeasy Total RNA Extraction kit (Qiagen), and treated with DNase I (Qiagen) for 15 min at room temperature to remove residual genomic DNA. RNA quality was analyzed using a Bioanalyzer 2100 (Agilent Technologies), producing RIN values between 9.5 and 10.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 500 ng of total RNA was used to synthesize cRNA with a GeneChip 3' IVT Express Kit (Affymetrix).
| | Sample_hyb_protocol | After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
| | Sample_scan_protocol | The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
| | Sample_data_processing | Data were processed using RMA using GeneChip Analysis Suite.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yoshiaki,,Tabuchi
| | Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| | Sample_contact_laboratory | Division of Molecular Genetics Research
| | Sample_contact_department | Life Science Research Center
| | Sample_contact_institute | University of Toyama
| | Sample_contact_address | 2630 Sugitani
| | Sample_contact_city | Toyama
| | Sample_contact_state | Toyama
| | Sample_contact_zip/postal_code | 930-0194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1072nnn/GSM1072428/suppl/GSM1072428_HSC-3-1h.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1072nnn/GSM1072428/suppl/GSM1072428_HSC-3-1h.CHP.gz
| | Sample_series_id | GSE43862
| | Sample_data_row_count | 54675
| | |
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GSM1072429 | GPL570 |
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HSC-3 cells-MHT 3h
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Human oral squamous cell carcinoma cell line HSC-3, MHT, 3h
|
cell line: HSC-3
cell type: oral squamous cell carcinoma cells
morphology: epithelial-like
life span: infinite
treatment: heat stress
time point: 3 hr
|
HSC-3-3h
HSC-3 cells treated with heat stress at 41°C and cultured for 3 h.
HSC-3 cells are derived from the tongue of a 64-year-old male.
|
| Sample_geo_accession | GSM1072429
| | Sample_status | Public on Jan 30 2013
| | Sample_submission_date | Jan 29 2013
| | Sample_last_update_date | Jan 30 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MHT treatment was carried out by immersing plastic culture vessels containing the attached cells in a water bath at 41°C for 30 min. After heat treatment, the cells were incubated for 0-3 h at 37°C.
| | Sample_growth_protocol_ch1 | HSC-3 cells were cultured in Eagle's Minimum Essential Medium supplemented with 10% fetal bovine serum at 37°C in humidified air with 5% CO2 and 95% air.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells using an RNeasy Total RNA Extraction kit (Qiagen), and treated with DNase I (Qiagen) for 15 min at room temperature to remove residual genomic DNA. RNA quality was analyzed using a Bioanalyzer 2100 (Agilent Technologies), producing RIN values between 9.5 and 10.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 500 ng of total RNA was used to synthesize cRNA with a GeneChip 3' IVT Express Kit (Affymetrix).
| | Sample_hyb_protocol | After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
| | Sample_scan_protocol | The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
| | Sample_data_processing | Data were processed using RMA using GeneChip Analysis Suite.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yoshiaki,,Tabuchi
| | Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| | Sample_contact_laboratory | Division of Molecular Genetics Research
| | Sample_contact_department | Life Science Research Center
| | Sample_contact_institute | University of Toyama
| | Sample_contact_address | 2630 Sugitani
| | Sample_contact_city | Toyama
| | Sample_contact_state | Toyama
| | Sample_contact_zip/postal_code | 930-0194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1072nnn/GSM1072429/suppl/GSM1072429_HSC-3-3h.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1072nnn/GSM1072429/suppl/GSM1072429_HSC-3-3h.CHP.gz
| | Sample_series_id | GSE43862
| | Sample_data_row_count | 54675
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