Search results for the GEO ID: GSE43884  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1074059 | GPL570 |
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A2780-shUCHL1
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ovarian cancer cell line A2780-shUCHL1
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cell line: A2780
genotype/variation: UCHL1 knockdown
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UCHL1 knocked down A2780
A73.mas5
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| Sample_geo_accession | GSM1074059
| | Sample_status | Public on Jan 30 2013
| | Sample_submission_date | Jan 30 2013
| | Sample_last_update_date | Jan 30 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | UCHL1 was knocked down with shUCHL1 or non-target shRNA in A2780
| | Sample_growth_protocol_ch1 | cells were cultured in RPMI 1640 with 10% FBS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | total RNA was extracted with Trizol according to the manufacturer's protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Start with 250ng total RNA, and Biotinylated cRNA were prepared according to the standard Affymetrix protocol, in which, GeneChip 3 IVT Express Kit (Lot no.901228) is used in the experiment.
| | Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hours at 45 ℃ on GeneChip U133_plus 2.0 Array. GeneChips were washed and stained in the Affymetrix fluidics Station 450.
| | Sample_scan_protocol | The chips were scanned with the affymetrix genechip 7G plus, and AGCC is used for the image processing
| | Sample_data_processing | Genespring 10.0 is used for automatically normalizing the chip data and analyzing the fold change
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chengmeng,,Jin
| | Sample_contact_email | amengjcm@126.com
| | Sample_contact_department | SBP
| | Sample_contact_institute | ZCNI
| | Sample_contact_address | 268 kaixuan road
| | Sample_contact_city | Hangzhou
| | Sample_contact_state | zhejiang
| | Sample_contact_zip/postal_code | 310029
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1074nnn/GSM1074059/suppl/GSM1074059_A73.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1074nnn/GSM1074059/suppl/GSM1074059_A73.mas5.CHP.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1074nnn/GSM1074059/suppl/GSM1074059_A73.mas5.RPT.gz
| | Sample_series_id | GSE43884
| | Sample_data_row_count | 54675
| | |
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GSM1074060 | GPL570 |
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A2780-control
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ovarian cancer cell line A2780-control
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cell line: A2780
genotype/variation: control
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A2780 control
A-L.mas5
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| Sample_geo_accession | GSM1074060
| | Sample_status | Public on Jan 30 2013
| | Sample_submission_date | Jan 30 2013
| | Sample_last_update_date | Jan 30 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | UCHL1 was knocked down with shUCHL1 or non-target shRNA in A2780
| | Sample_growth_protocol_ch1 | cells were cultured in RPMI 1640 with 10% FBS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | total RNA was extracted with Trizol according to the manufacturer's protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Start with 250ng total RNA, and Biotinylated cRNA were prepared according to the standard Affymetrix protocol, in which, GeneChip 3 IVT Express Kit (Lot no.901228) is used in the experiment.
| | Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hours at 45 ℃ on GeneChip U133_plus 2.0 Array. GeneChips were washed and stained in the Affymetrix fluidics Station 450.
| | Sample_scan_protocol | The chips were scanned with the affymetrix genechip 7G plus, and AGCC is used for the image processing
| | Sample_data_processing | Genespring 10.0 is used for automatically normalizing the chip data and analyzing the fold change
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chengmeng,,Jin
| | Sample_contact_email | amengjcm@126.com
| | Sample_contact_department | SBP
| | Sample_contact_institute | ZCNI
| | Sample_contact_address | 268 kaixuan road
| | Sample_contact_city | Hangzhou
| | Sample_contact_state | zhejiang
| | Sample_contact_zip/postal_code | 310029
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1074nnn/GSM1074060/suppl/GSM1074060_A-L.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1074nnn/GSM1074060/suppl/GSM1074060_A-L.mas5.CHP.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1074nnn/GSM1074060/suppl/GSM1074060_A-L.mas5.RPT.gz
| | Sample_series_id | GSE43884
| | Sample_data_row_count | 54675
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