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Search results for the GEO ID: GSE43991

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Description

Characteristics

GSM1076145

GPL1261

non-transformed

p53-/- mouse fetal liver progenitor cells

cell: p53-/- mouse fetal liver progenitor cells transformation: non-transformed

Gene expression data from the liver progenitor cells

Sample_geo_accession	GSM1076145
Sample_status	Public on Feb 02 2013
Sample_submission_date	Feb 01 2013
Sample_last_update_date	Feb 02 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	Liver progenitor cells were cultured in a maintenance medium containing 1:1 DMEM:F12 (GIBCO, Grand Island, NY) with 10% fetal bovine serum (GIBCO) and the following additives: dexamethasone (1×10−7 M; Sigma-Aldrich, St. Louis, MO), nicotinic acid (0.01 M; Sigma-Aldrich), 2-mercaptoethanol (50 mM; Sigma-Aldrich), 1× ITS liquid media supplement (Sigma-Aldrich), EGF (20 ng/ml; R&D Systems, Minneapolis, MN), HGF (50 ng/ml; R&D Systems), and penicillin/streptomycin (1% [v/v]; GIBCO).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1ug total RNA (Expression Analysis Technical Manual, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip mouse 430 2.0 Genome Array in the Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the affymetrix scanner 3000 7G.The scanned images were first assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of AGCC.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1261
Sample_contact_name	Yongzhong,,Liu
Sample_contact_email	liuyzg@shsci.org
Sample_contact_institute	Shanghai Cancer Institute
Sample_contact_address	Xietu Road 2200, 25
Sample_contact_city	Shanghai
Sample_contact_zip/postal_code	200031
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1076nnn/GSM1076145/suppl/GSM1076145_non-transformed.CEL.gz
Sample_series_id	GSE43991
Sample_data_row_count	45101

GSM1076146

GPL1261

H-RasV12

p53-/- mouse fetal liver progenitor cells transformed with H-RasV12

cell: p53-/- mouse fetal liver progenitor cells transformed with H-RasV12 transformation: H-RasV12

Gene expression data from the liver progenitor cells transformed with H-RasV12

Sample_geo_accession	GSM1076146
Sample_status	Public on Feb 02 2013
Sample_submission_date	Feb 01 2013
Sample_last_update_date	Feb 02 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	Liver progenitor cells were cultured in a maintenance medium containing 1:1 DMEM:F12 (GIBCO, Grand Island, NY) with 10% fetal bovine serum (GIBCO) and the following additives: dexamethasone (1×10−7 M; Sigma-Aldrich, St. Louis, MO), nicotinic acid (0.01 M; Sigma-Aldrich), 2-mercaptoethanol (50 mM; Sigma-Aldrich), 1× ITS liquid media supplement (Sigma-Aldrich), EGF (20 ng/ml; R&D Systems, Minneapolis, MN), HGF (50 ng/ml; R&D Systems), and penicillin/streptomycin (1% [v/v]; GIBCO).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1ug total RNA (Expression Analysis Technical Manual, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip mouse 430 2.0 Genome Array in the Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the affymetrix scanner 3000 7G.The scanned images were first assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of AGCC.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1261
Sample_contact_name	Yongzhong,,Liu
Sample_contact_email	liuyzg@shsci.org
Sample_contact_institute	Shanghai Cancer Institute
Sample_contact_address	Xietu Road 2200, 25
Sample_contact_city	Shanghai
Sample_contact_zip/postal_code	200031
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1076nnn/GSM1076146/suppl/GSM1076146_H-RasV12.CEL.gz
Sample_series_id	GSE43991
Sample_data_row_count	45101

GSM1076147

GPL1261

myr-Akt

p53-/- mouse fetal liver progenitor cells transformed with myr-Akt

cell: p53-/- mouse fetal liver progenitor cells transformed with myr-Akt transformation: myr-Akt

Gene expression data from the liver progenitor cells transformed with myr-Akt

Sample_geo_accession	GSM1076147
Sample_status	Public on Feb 02 2013
Sample_submission_date	Feb 01 2013
Sample_last_update_date	Feb 02 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	Liver progenitor cells were cultured in a maintenance medium containing 1:1 DMEM:F12 (GIBCO, Grand Island, NY) with 10% fetal bovine serum (GIBCO) and the following additives: dexamethasone (1×10−7 M; Sigma-Aldrich, St. Louis, MO), nicotinic acid (0.01 M; Sigma-Aldrich), 2-mercaptoethanol (50 mM; Sigma-Aldrich), 1× ITS liquid media supplement (Sigma-Aldrich), EGF (20 ng/ml; R&D Systems, Minneapolis, MN), HGF (50 ng/ml; R&D Systems), and penicillin/streptomycin (1% [v/v]; GIBCO).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1ug total RNA (Expression Analysis Technical Manual, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip mouse 430 2.0 Genome Array in the Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the affymetrix scanner 3000 7G.The scanned images were first assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of AGCC.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1261
Sample_contact_name	Yongzhong,,Liu
Sample_contact_email	liuyzg@shsci.org
Sample_contact_institute	Shanghai Cancer Institute
Sample_contact_address	Xietu Road 2200, 25
Sample_contact_city	Shanghai
Sample_contact_zip/postal_code	200031
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1076nnn/GSM1076147/suppl/GSM1076147_myr-Akt.CEL.gz
Sample_series_id	GSE43991
Sample_data_row_count	45101

GSM1076148

GPL1261

c-Myc

p53-/- mouse fetal liver progenitor cells transformed with c-Myc

cell: p53-/- mouse fetal liver progenitor cells transformed with c-Myc transformation: c-Myc

Gene expression data from the liver progenitor cells transformed with c-Myc

Sample_geo_accession	GSM1076148
Sample_status	Public on Feb 02 2013
Sample_submission_date	Feb 01 2013
Sample_last_update_date	Feb 02 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	Liver progenitor cells were cultured in a maintenance medium containing 1:1 DMEM:F12 (GIBCO, Grand Island, NY) with 10% fetal bovine serum (GIBCO) and the following additives: dexamethasone (1×10−7 M; Sigma-Aldrich, St. Louis, MO), nicotinic acid (0.01 M; Sigma-Aldrich), 2-mercaptoethanol (50 mM; Sigma-Aldrich), 1× ITS liquid media supplement (Sigma-Aldrich), EGF (20 ng/ml; R&D Systems, Minneapolis, MN), HGF (50 ng/ml; R&D Systems), and penicillin/streptomycin (1% [v/v]; GIBCO).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1ug total RNA (Expression Analysis Technical Manual, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip mouse 430 2.0 Genome Array in the Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the affymetrix scanner 3000 7G.The scanned images were first assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of AGCC.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1261
Sample_contact_name	Yongzhong,,Liu
Sample_contact_email	liuyzg@shsci.org
Sample_contact_institute	Shanghai Cancer Institute
Sample_contact_address	Xietu Road 2200, 25
Sample_contact_city	Shanghai
Sample_contact_zip/postal_code	200031
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1076nnn/GSM1076148/suppl/GSM1076148_c-Myc.CEL.gz
Sample_series_id	GSE43991
Sample_data_row_count	45101

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