Result

Search results for the GEO ID: GSE43998

(Click on the check boxes provided under "Select for analysis", to initiate grouping)

(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down)

GSM ID

GPL ID

Select for analysis

Title

Source name

Description

Characteristics

GSM1076317

GPL570

K562 p42-ER_estradiol treated_biological rep1

K562 p42-C/EBPa-ER, estradiol treated, 6 hours

cell line: K562 cells stably transfected with p42 C/EBPa-ER treatment: beta-estradiol treatment duration: 6 hours

K562 p42-C/EBPa-ER E2 1 Gene expression data following translocation of the inducible C/EBPa-ER protein into the nucleus with beta-estradiol.

Sample_geo_accession	GSM1076317
Sample_status	Public on Apr 01 2013
Sample_submission_date	Feb 01 2013
Sample_last_update_date	Apr 01 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Induction of nuclear localization of C/EBPa-ER fusion protein was achieved by addition of 1 uM beta-estradiol as indicated into the culture medium. Vehicle control-treated cells were treated with 1uM ethanol.
Sample_growth_protocol_ch1	K562 cells stably expressing p42-C/EBPa-ER were maintained in phenol red-free RPMI medium supplemented with 10% charcoal-stripped FBS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagen RNeasy Mini kit according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Briefly, for each sample, 200ng of total RNA in a volume of 5µl was amplified using the Applause WT Amp ST, RNA Amplification System (NuGen, San Carlos, CA) and labelled with the Encore Biotin Module, Post–Amplification System (NuGen).
Sample_hyb_protocol	A total of 2.5µg of labelled cDNA, along with GeneChip Hybridization Control reagents (Affymetrix), was injected into an Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. The chips were incubated for 18 hours at 45oC and rotated at 60rpm to allow hybridization. The chips were then washed and stained using the GeneChip Hybridization Wash and Stain Kit (Affymetrix) using the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner GCS 3000 with the GeneChip Operating software version 1.4.0.036.
Sample_data_processing	CEL files were normalized using the BRB-Array's RMA preprocessing function. Prediction Analysis of Microarrays (PAM, Stanford University) was used to identify probe sets which represent genes differentially expressed between two conditions.
Sample_platform_id	GPL570
Sample_contact_name	Daniel,G,Tenen
Sample_contact_email	csibox6@nus.edu.sg
Sample_contact_department	Cancer Science Institute of Singapore
Sample_contact_institute	National University of Singapore
Sample_contact_address	14 Medical Drive, #12-01
Sample_contact_city	Singapore
Sample_contact_zip/postal_code	117599
Sample_contact_country	Singapore
Sample_contact_web_link	http://www.csi.nus.edu.sg
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1076nnn/GSM1076317/suppl/GSM1076317_p42_E2_1-Plus.CEL.gz
Sample_series_id	GSE43998
Sample_data_row_count	54675

GSM1076318

GPL570

K562 p42-ER_estradiol treated_biological rep2

K562 p42-C/EBPa-ER, estradiol treated, 6 hours

cell line: K562 cells stably transfected with p42 C/EBPa-ER treatment: beta-estradiol treatment duration: 6 hours

K562 p42-C/EBPa-ER E2 2 Gene expression data following translocation of the inducible C/EBPa-ER protein into the nucleus with beta-estradiol.

Sample_geo_accession	GSM1076318
Sample_status	Public on Apr 01 2013
Sample_submission_date	Feb 01 2013
Sample_last_update_date	Apr 01 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Induction of nuclear localization of C/EBPa-ER fusion protein was achieved by addition of 1 uM beta-estradiol as indicated into the culture medium. Vehicle control-treated cells were treated with 1uM ethanol.
Sample_growth_protocol_ch1	K562 cells stably expressing p42-C/EBPa-ER were maintained in phenol red-free RPMI medium supplemented with 10% charcoal-stripped FBS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagen RNeasy Mini kit according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Briefly, for each sample, 200ng of total RNA in a volume of 5µl was amplified using the Applause WT Amp ST, RNA Amplification System (NuGen, San Carlos, CA) and labelled with the Encore Biotin Module, Post–Amplification System (NuGen).
Sample_hyb_protocol	A total of 2.5µg of labelled cDNA, along with GeneChip Hybridization Control reagents (Affymetrix), was injected into an Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. The chips were incubated for 18 hours at 45oC and rotated at 60rpm to allow hybridization. The chips were then washed and stained using the GeneChip Hybridization Wash and Stain Kit (Affymetrix) using the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner GCS 3000 with the GeneChip Operating software version 1.4.0.036.
Sample_data_processing	CEL files were normalized using the BRB-Array's RMA preprocessing function. Prediction Analysis of Microarrays (PAM, Stanford University) was used to identify probe sets which represent genes differentially expressed between two conditions.
Sample_platform_id	GPL570
Sample_contact_name	Daniel,G,Tenen
Sample_contact_email	csibox6@nus.edu.sg
Sample_contact_department	Cancer Science Institute of Singapore
Sample_contact_institute	National University of Singapore
Sample_contact_address	14 Medical Drive, #12-01
Sample_contact_city	Singapore
Sample_contact_zip/postal_code	117599
Sample_contact_country	Singapore
Sample_contact_web_link	http://www.csi.nus.edu.sg
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1076nnn/GSM1076318/suppl/GSM1076318_p42_E2_2-Plus.CEL.gz
Sample_series_id	GSE43998
Sample_data_row_count	54675

GSM1076319

GPL570

K562 p42-ER_estradiol treated_biological rep3

K562 p42-C/EBPa-ER, estradiol treated, 6 hours

cell line: K562 cells stably transfected with p42 C/EBPa-ER treatment: beta-estradiol treatment duration: 6 hours

K562 p42-C/EBPa-ER E2 3 Gene expression data following translocation of the inducible C/EBPa-ER protein into the nucleus with beta-estradiol.

Sample_geo_accession	GSM1076319
Sample_status	Public on Apr 01 2013
Sample_submission_date	Feb 01 2013
Sample_last_update_date	Apr 01 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Induction of nuclear localization of C/EBPa-ER fusion protein was achieved by addition of 1 uM beta-estradiol as indicated into the culture medium. Vehicle control-treated cells were treated with 1uM ethanol.
Sample_growth_protocol_ch1	K562 cells stably expressing p42-C/EBPa-ER were maintained in phenol red-free RPMI medium supplemented with 10% charcoal-stripped FBS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagen RNeasy Mini kit according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Briefly, for each sample, 200ng of total RNA in a volume of 5µl was amplified using the Applause WT Amp ST, RNA Amplification System (NuGen, San Carlos, CA) and labelled with the Encore Biotin Module, Post–Amplification System (NuGen).
Sample_hyb_protocol	A total of 2.5µg of labelled cDNA, along with GeneChip Hybridization Control reagents (Affymetrix), was injected into an Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. The chips were incubated for 18 hours at 45oC and rotated at 60rpm to allow hybridization. The chips were then washed and stained using the GeneChip Hybridization Wash and Stain Kit (Affymetrix) using the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner GCS 3000 with the GeneChip Operating software version 1.4.0.036.
Sample_data_processing	CEL files were normalized using the BRB-Array's RMA preprocessing function. Prediction Analysis of Microarrays (PAM, Stanford University) was used to identify probe sets which represent genes differentially expressed between two conditions.
Sample_platform_id	GPL570
Sample_contact_name	Daniel,G,Tenen
Sample_contact_email	csibox6@nus.edu.sg
Sample_contact_department	Cancer Science Institute of Singapore
Sample_contact_institute	National University of Singapore
Sample_contact_address	14 Medical Drive, #12-01
Sample_contact_city	Singapore
Sample_contact_zip/postal_code	117599
Sample_contact_country	Singapore
Sample_contact_web_link	http://www.csi.nus.edu.sg
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1076nnn/GSM1076319/suppl/GSM1076319_p42_E2_3-Plus.CEL.gz
Sample_series_id	GSE43998
Sample_data_row_count	54675

GSM1076320

GPL570

K562 p42-ER_estradiol treated_biological rep4

K562 p42-C/EBPa-ER, estradiol treated, 6 hours

cell line: K562 cells stably transfected with p42 C/EBPa-ER treatment: beta-estradiol treatment duration: 6 hours

K562 p42-C/EBPa-ER E2 4 Gene expression data following translocation of the inducible C/EBPa-ER protein into the nucleus with beta-estradiol.

Sample_geo_accession	GSM1076320
Sample_status	Public on Apr 01 2013
Sample_submission_date	Feb 01 2013
Sample_last_update_date	Apr 01 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Induction of nuclear localization of C/EBPa-ER fusion protein was achieved by addition of 1 uM beta-estradiol as indicated into the culture medium. Vehicle control-treated cells were treated with 1uM ethanol.
Sample_growth_protocol_ch1	K562 cells stably expressing p42-C/EBPa-ER were maintained in phenol red-free RPMI medium supplemented with 10% charcoal-stripped FBS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagen RNeasy Mini kit according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Briefly, for each sample, 200ng of total RNA in a volume of 5µl was amplified using the Applause WT Amp ST, RNA Amplification System (NuGen, San Carlos, CA) and labelled with the Encore Biotin Module, Post–Amplification System (NuGen).
Sample_hyb_protocol	A total of 2.5µg of labelled cDNA, along with GeneChip Hybridization Control reagents (Affymetrix), was injected into an Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. The chips were incubated for 18 hours at 45oC and rotated at 60rpm to allow hybridization. The chips were then washed and stained using the GeneChip Hybridization Wash and Stain Kit (Affymetrix) using the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner GCS 3000 with the GeneChip Operating software version 1.4.0.036.
Sample_data_processing	CEL files were normalized using the BRB-Array's RMA preprocessing function. Prediction Analysis of Microarrays (PAM, Stanford University) was used to identify probe sets which represent genes differentially expressed between two conditions.
Sample_platform_id	GPL570
Sample_contact_name	Daniel,G,Tenen
Sample_contact_email	csibox6@nus.edu.sg
Sample_contact_department	Cancer Science Institute of Singapore
Sample_contact_institute	National University of Singapore
Sample_contact_address	14 Medical Drive, #12-01
Sample_contact_city	Singapore
Sample_contact_zip/postal_code	117599
Sample_contact_country	Singapore
Sample_contact_web_link	http://www.csi.nus.edu.sg
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1076nnn/GSM1076320/suppl/GSM1076320_p42_E2_4-Plus.CEL.gz
Sample_series_id	GSE43998
Sample_data_row_count	54675

GSM1076321

GPL570

K562 p42-ER_vehicle control treated_biological rep1

K562 p42-C/EBPa-ER, vehicle control treated, 6 hours

cell line: K562 cells stably transfected with p42 C/EBPa-ER treatment: ethanol vehicle control treatment duration: 6 hours

K562 p42-C/EBPa-ER VC 1 Gene expression data following control treatment of the inducible C/EBPa-ER protein with vehicle control.

Sample_geo_accession	GSM1076321
Sample_status	Public on Apr 01 2013
Sample_submission_date	Feb 01 2013
Sample_last_update_date	Apr 01 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Induction of nuclear localization of C/EBPa-ER fusion protein was achieved by addition of 1 uM beta-estradiol as indicated into the culture medium. Vehicle control-treated cells were treated with 1uM ethanol.
Sample_growth_protocol_ch1	K562 cells stably expressing p42-C/EBPa-ER were maintained in phenol red-free RPMI medium supplemented with 10% charcoal-stripped FBS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagen RNeasy Mini kit according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Briefly, for each sample, 200ng of total RNA in a volume of 5µl was amplified using the Applause WT Amp ST, RNA Amplification System (NuGen, San Carlos, CA) and labelled with the Encore Biotin Module, Post–Amplification System (NuGen).
Sample_hyb_protocol	A total of 2.5µg of labelled cDNA, along with GeneChip Hybridization Control reagents (Affymetrix), was injected into an Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. The chips were incubated for 18 hours at 45oC and rotated at 60rpm to allow hybridization. The chips were then washed and stained using the GeneChip Hybridization Wash and Stain Kit (Affymetrix) using the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner GCS 3000 with the GeneChip Operating software version 1.4.0.036.
Sample_data_processing	CEL files were normalized using the BRB-Array's RMA preprocessing function. Prediction Analysis of Microarrays (PAM, Stanford University) was used to identify probe sets which represent genes differentially expressed between two conditions.
Sample_platform_id	GPL570
Sample_contact_name	Daniel,G,Tenen
Sample_contact_email	csibox6@nus.edu.sg
Sample_contact_department	Cancer Science Institute of Singapore
Sample_contact_institute	National University of Singapore
Sample_contact_address	14 Medical Drive, #12-01
Sample_contact_city	Singapore
Sample_contact_zip/postal_code	117599
Sample_contact_country	Singapore
Sample_contact_web_link	http://www.csi.nus.edu.sg
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1076nnn/GSM1076321/suppl/GSM1076321_p42_VC_1-Plus.CEL.gz
Sample_series_id	GSE43998
Sample_data_row_count	54675

GSM1076322

GPL570

K562 p42-ER_vehicle control treated_biological rep2

K562 p42-C/EBPa-ER, vehicle control treated, 6 hours

cell line: K562 cells stably transfected with p42 C/EBPa-ER treatment: ethanol vehicle control treatment duration: 6 hours

K562 p42-C/EBPa-ER VC 2 Gene expression data following control treatment of the inducible C/EBPa-ER protein with vehicle control.

Sample_geo_accession	GSM1076322
Sample_status	Public on Apr 01 2013
Sample_submission_date	Feb 01 2013
Sample_last_update_date	Apr 01 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Induction of nuclear localization of C/EBPa-ER fusion protein was achieved by addition of 1 uM beta-estradiol as indicated into the culture medium. Vehicle control-treated cells were treated with 1uM ethanol.
Sample_growth_protocol_ch1	K562 cells stably expressing p42-C/EBPa-ER were maintained in phenol red-free RPMI medium supplemented with 10% charcoal-stripped FBS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagen RNeasy Mini kit according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Briefly, for each sample, 200ng of total RNA in a volume of 5µl was amplified using the Applause WT Amp ST, RNA Amplification System (NuGen, San Carlos, CA) and labelled with the Encore Biotin Module, Post–Amplification System (NuGen).
Sample_hyb_protocol	A total of 2.5µg of labelled cDNA, along with GeneChip Hybridization Control reagents (Affymetrix), was injected into an Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. The chips were incubated for 18 hours at 45oC and rotated at 60rpm to allow hybridization. The chips were then washed and stained using the GeneChip Hybridization Wash and Stain Kit (Affymetrix) using the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner GCS 3000 with the GeneChip Operating software version 1.4.0.036.
Sample_data_processing	CEL files were normalized using the BRB-Array's RMA preprocessing function. Prediction Analysis of Microarrays (PAM, Stanford University) was used to identify probe sets which represent genes differentially expressed between two conditions.
Sample_platform_id	GPL570
Sample_contact_name	Daniel,G,Tenen
Sample_contact_email	csibox6@nus.edu.sg
Sample_contact_department	Cancer Science Institute of Singapore
Sample_contact_institute	National University of Singapore
Sample_contact_address	14 Medical Drive, #12-01
Sample_contact_city	Singapore
Sample_contact_zip/postal_code	117599
Sample_contact_country	Singapore
Sample_contact_web_link	http://www.csi.nus.edu.sg
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1076nnn/GSM1076322/suppl/GSM1076322_p42_VC_2-Plus.CEL.gz
Sample_series_id	GSE43998
Sample_data_row_count	54675

GSM1076323

GPL570

K562 p42-ER_vehicle control treated_biological rep3

K562 p42-C/EBPa-ER, vehicle control treated, 6 hours

cell line: K562 cells stably transfected with p42 C/EBPa-ER treatment: ethanol vehicle control treatment duration: 6 hours

K562 p42-C/EBPa-ER VC 3 Gene expression data following control treatment of the inducible C/EBPa-ER protein with vehicle control.

Sample_geo_accession	GSM1076323
Sample_status	Public on Apr 01 2013
Sample_submission_date	Feb 01 2013
Sample_last_update_date	Apr 01 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Induction of nuclear localization of C/EBPa-ER fusion protein was achieved by addition of 1 uM beta-estradiol as indicated into the culture medium. Vehicle control-treated cells were treated with 1uM ethanol.
Sample_growth_protocol_ch1	K562 cells stably expressing p42-C/EBPa-ER were maintained in phenol red-free RPMI medium supplemented with 10% charcoal-stripped FBS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagen RNeasy Mini kit according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Briefly, for each sample, 200ng of total RNA in a volume of 5µl was amplified using the Applause WT Amp ST, RNA Amplification System (NuGen, San Carlos, CA) and labelled with the Encore Biotin Module, Post–Amplification System (NuGen).
Sample_hyb_protocol	A total of 2.5µg of labelled cDNA, along with GeneChip Hybridization Control reagents (Affymetrix), was injected into an Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. The chips were incubated for 18 hours at 45oC and rotated at 60rpm to allow hybridization. The chips were then washed and stained using the GeneChip Hybridization Wash and Stain Kit (Affymetrix) using the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner GCS 3000 with the GeneChip Operating software version 1.4.0.036.
Sample_data_processing	CEL files were normalized using the BRB-Array's RMA preprocessing function. Prediction Analysis of Microarrays (PAM, Stanford University) was used to identify probe sets which represent genes differentially expressed between two conditions.
Sample_platform_id	GPL570
Sample_contact_name	Daniel,G,Tenen
Sample_contact_email	csibox6@nus.edu.sg
Sample_contact_department	Cancer Science Institute of Singapore
Sample_contact_institute	National University of Singapore
Sample_contact_address	14 Medical Drive, #12-01
Sample_contact_city	Singapore
Sample_contact_zip/postal_code	117599
Sample_contact_country	Singapore
Sample_contact_web_link	http://www.csi.nus.edu.sg
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1076nnn/GSM1076323/suppl/GSM1076323_p42_VC_3-Plus.CEL.gz
Sample_series_id	GSE43998
Sample_data_row_count	54675

GSM1076324

GPL570

K562 p42-ER_vehicle control treated_biological rep4

K562 p42-C/EBPa-ER, vehicle control treated, 6 hours

cell line: K562 cells stably transfected with p42 C/EBPa-ER treatment: ethanol vehicle control treatment duration: 6 hours

K562 p42-C/EBPa-ER VC 4 Gene expression data following control treatment of the inducible C/EBPa-ER protein with vehicle control.

Sample_geo_accession	GSM1076324
Sample_status	Public on Apr 01 2013
Sample_submission_date	Feb 01 2013
Sample_last_update_date	Apr 01 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Induction of nuclear localization of C/EBPa-ER fusion protein was achieved by addition of 1 uM beta-estradiol as indicated into the culture medium. Vehicle control-treated cells were treated with 1uM ethanol.
Sample_growth_protocol_ch1	K562 cells stably expressing p42-C/EBPa-ER were maintained in phenol red-free RPMI medium supplemented with 10% charcoal-stripped FBS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagen RNeasy Mini kit according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Briefly, for each sample, 200ng of total RNA in a volume of 5µl was amplified using the Applause WT Amp ST, RNA Amplification System (NuGen, San Carlos, CA) and labelled with the Encore Biotin Module, Post–Amplification System (NuGen).
Sample_hyb_protocol	A total of 2.5µg of labelled cDNA, along with GeneChip Hybridization Control reagents (Affymetrix), was injected into an Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. The chips were incubated for 18 hours at 45oC and rotated at 60rpm to allow hybridization. The chips were then washed and stained using the GeneChip Hybridization Wash and Stain Kit (Affymetrix) using the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner GCS 3000 with the GeneChip Operating software version 1.4.0.036.
Sample_data_processing	CEL files were normalized using the BRB-Array's RMA preprocessing function. Prediction Analysis of Microarrays (PAM, Stanford University) was used to identify probe sets which represent genes differentially expressed between two conditions.
Sample_platform_id	GPL570
Sample_contact_name	Daniel,G,Tenen
Sample_contact_email	csibox6@nus.edu.sg
Sample_contact_department	Cancer Science Institute of Singapore
Sample_contact_institute	National University of Singapore
Sample_contact_address	14 Medical Drive, #12-01
Sample_contact_city	Singapore
Sample_contact_zip/postal_code	117599
Sample_contact_country	Singapore
Sample_contact_web_link	http://www.csi.nus.edu.sg
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1076nnn/GSM1076324/suppl/GSM1076324_p42_VC_4-Plus.CEL.gz
Sample_series_id	GSE43998
Sample_data_row_count	54675

Make groups for comparisons

(2 groups will be compared at a time)

Select GSMs and click on "Add groups"

Enter the group name here:

Select expression type

Transcripts profile based on;

A. Differential status (Up/Down regulation)

B. Absolute calls (Transcribed/Not-detected)

Filter results by number of probes