Search results for the GEO ID: GSE44126  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1079344 | GPL570 |
|
CD4+CD40L+, biological rep1
|
PBMC stimulated with PMA/Ionomycin for 6 h
|
tissue: Blood
gender: Male
cell population: CD4+CD45RA-CD40L+
|
Gene expression data of human CD4+CD40L+ stimulated with PMA/Ionomycin for 6 h
|
| Sample_geo_accession | GSM1079344
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Feb 06 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | PBMCs were isolated by Ficoll gradient from buffy coat. CD4+ and CD8+ T cells were isolated via MACS, The cells were stimulated with PMA/Ionomycin for 6 h, and subsequently sorted into the CD4+CD45RA-CD40L+, CD8+CD45RA-CD40L-, CD8+CD45RA-CD40L+ populations by FACS-sort
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 3-5 ug total RNA according to the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_hyb_protocol | 15 µg fragmented cRNA was hybridized to the Affymetrix HG-U133Plus2 gene chip in a Hybridization Oven 640, washed and stained in the Fluidics Station 400 (Affymetrix), according to procedure 2 described in the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_scan_protocol | The arrays were scanned with an Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Chip data were analyzed with GeneChip Operating Software (GCOS 1.1; Affymetrix) default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 200
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulrik,,Stervbo
| | Sample_contact_email | ulrik.stervbo@gmail.com
| | Sample_contact_laboratory | AG Thiel
| | Sample_contact_institute | BCRT
| | Sample_contact_address | Föhrer Straße 15
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1079nnn/GSM1079344/suppl/GSM1079344_BC_031_CD4+_CD40L+_2_133P.CEL.gz
| | Sample_series_id | GSE44126
| | Sample_data_row_count | 54675
| | |
|
GSM1079345 | GPL570 |
|
CD4+CD40L+, biological rep2
|
PBMC stimulated with PMA/Ionomycin for 6 h
|
tissue: Blood
gender: Male
cell population: CD4+CD45RA-CD40L+
|
Gene expression data of human CD4+CD40L+ stimulated with PMA/Ionomycin for 6 h
|
| Sample_geo_accession | GSM1079345
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Feb 06 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | PBMCs were isolated by Ficoll gradient from buffy coat. CD4+ and CD8+ T cells were isolated via MACS, The cells were stimulated with PMA/Ionomycin for 6 h, and subsequently sorted into the CD4+CD45RA-CD40L+, CD8+CD45RA-CD40L-, CD8+CD45RA-CD40L+ populations by FACS-sort
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 3-5 ug total RNA according to the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_hyb_protocol | 15 µg fragmented cRNA was hybridized to the Affymetrix HG-U133Plus2 gene chip in a Hybridization Oven 640, washed and stained in the Fluidics Station 400 (Affymetrix), according to procedure 2 described in the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_scan_protocol | The arrays were scanned with an Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Chip data were analyzed with GeneChip Operating Software (GCOS 1.1; Affymetrix) default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 200
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulrik,,Stervbo
| | Sample_contact_email | ulrik.stervbo@gmail.com
| | Sample_contact_laboratory | AG Thiel
| | Sample_contact_institute | BCRT
| | Sample_contact_address | Föhrer Straße 15
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1079nnn/GSM1079345/suppl/GSM1079345_BC_036_CD4+_CD40L+_3_133P.CEL.gz
| | Sample_series_id | GSE44126
| | Sample_data_row_count | 54675
| | |
|
GSM1079346 | GPL570 |
|
CD4+CD40L+, biological rep3
|
PBMC stimulated with PMA/Ionomycin for 6 h
|
tissue: Blood
gender: Male
cell population: CD4+CD45RA-CD40L+
|
Gene expression data of human CD4+CD40L+ stimulated with PMA/Ionomycin for 6 h
|
| Sample_geo_accession | GSM1079346
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Feb 06 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | PBMCs were isolated by Ficoll gradient from buffy coat. CD4+ and CD8+ T cells were isolated via MACS, The cells were stimulated with PMA/Ionomycin for 6 h, and subsequently sorted into the CD4+CD45RA-CD40L+, CD8+CD45RA-CD40L-, CD8+CD45RA-CD40L+ populations by FACS-sort
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 3-5 ug total RNA according to the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_hyb_protocol | 15 µg fragmented cRNA was hybridized to the Affymetrix HG-U133Plus2 gene chip in a Hybridization Oven 640, washed and stained in the Fluidics Station 400 (Affymetrix), according to procedure 2 described in the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_scan_protocol | The arrays were scanned with an Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Chip data were analyzed with GeneChip Operating Software (GCOS 1.1; Affymetrix) default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 200
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulrik,,Stervbo
| | Sample_contact_email | ulrik.stervbo@gmail.com
| | Sample_contact_laboratory | AG Thiel
| | Sample_contact_institute | BCRT
| | Sample_contact_address | Föhrer Straße 15
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1079nnn/GSM1079346/suppl/GSM1079346_BC_077_CD4+_CD40L+_5_133P.CEL.gz
| | Sample_series_id | GSE44126
| | Sample_data_row_count | 54675
| | |
|
GSM1079347 | GPL570 |
|
CD4+CD40L+, biological rep4
|
PBMC stimulated with PMA/Ionomycin for 6 h
|
tissue: Blood
gender: Female
cell population: CD4+CD45RA-CD40L+
|
Gene expression data of human CD4+CD40L+ stimulated with PMA/Ionomycin for 6 h
|
| Sample_geo_accession | GSM1079347
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Feb 06 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | PBMCs were isolated by Ficoll gradient from buffy coat. CD4+ and CD8+ T cells were isolated via MACS, The cells were stimulated with PMA/Ionomycin for 6 h, and subsequently sorted into the CD4+CD45RA-CD40L+, CD8+CD45RA-CD40L-, CD8+CD45RA-CD40L+ populations by FACS-sort
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 3-5 ug total RNA according to the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_hyb_protocol | 15 µg fragmented cRNA was hybridized to the Affymetrix HG-U133Plus2 gene chip in a Hybridization Oven 640, washed and stained in the Fluidics Station 400 (Affymetrix), according to procedure 2 described in the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_scan_protocol | The arrays were scanned with an Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Chip data were analyzed with GeneChip Operating Software (GCOS 1.1; Affymetrix) default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 200
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulrik,,Stervbo
| | Sample_contact_email | ulrik.stervbo@gmail.com
| | Sample_contact_laboratory | AG Thiel
| | Sample_contact_institute | BCRT
| | Sample_contact_address | Föhrer Straße 15
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1079nnn/GSM1079347/suppl/GSM1079347_BC_229_CD4+_CD40L+_4_133P.CEL.gz
| | Sample_series_id | GSE44126
| | Sample_data_row_count | 54675
| | |
|
GSM1079348 | GPL570 |
|
CD4+CD40L+, biological rep5
|
PBMC stimulated with PMA/Ionomycin for 6 h
|
tissue: Blood
gender: Male
cell population: CD4+CD45RA-CD40L+
|
Gene expression data of human CD4+CD40L+ stimulated with PMA/Ionomycin for 6 h
|
| Sample_geo_accession | GSM1079348
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Feb 06 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | PBMCs were isolated by Ficoll gradient from buffy coat. CD4+ and CD8+ T cells were isolated via MACS, The cells were stimulated with PMA/Ionomycin for 6 h, and subsequently sorted into the CD4+CD45RA-CD40L+, CD8+CD45RA-CD40L-, CD8+CD45RA-CD40L+ populations by FACS-sort
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 3-5 ug total RNA according to the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_hyb_protocol | 15 µg fragmented cRNA was hybridized to the Affymetrix HG-U133Plus2 gene chip in a Hybridization Oven 640, washed and stained in the Fluidics Station 400 (Affymetrix), according to procedure 2 described in the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_scan_protocol | The arrays were scanned with an Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Chip data were analyzed with GeneChip Operating Software (GCOS 1.1; Affymetrix) default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 200
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulrik,,Stervbo
| | Sample_contact_email | ulrik.stervbo@gmail.com
| | Sample_contact_laboratory | AG Thiel
| | Sample_contact_institute | BCRT
| | Sample_contact_address | Föhrer Straße 15
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1079nnn/GSM1079348/suppl/GSM1079348_BC_782_CD4+_CD40L+_1_133P.CEL.gz
| | Sample_series_id | GSE44126
| | Sample_data_row_count | 54675
| | |
|
GSM1079349 | GPL570 |
|
CD8+CD40L-, biological rep1
|
PBMC stimulated with PMA/Ionomycin for 6 h
|
tissue: Blood
gender: Male
cell population: CD8+CD45RA-CD40L-
|
Gene expression data of human CD8+CD40L- stimulated with PMA/Ionomycin for 6 h
|
| Sample_geo_accession | GSM1079349
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Feb 06 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | PBMCs were isolated by Ficoll gradient from buffy coat. CD4+ and CD8+ T cells were isolated via MACS, The cells were stimulated with PMA/Ionomycin for 6 h, and subsequently sorted into the CD4+CD45RA-CD40L+, CD8+CD45RA-CD40L-, CD8+CD45RA-CD40L+ populations by FACS-sort
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 3-5 ug total RNA according to the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_hyb_protocol | 15 µg fragmented cRNA was hybridized to the Affymetrix HG-U133Plus2 gene chip in a Hybridization Oven 640, washed and stained in the Fluidics Station 400 (Affymetrix), according to procedure 2 described in the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_scan_protocol | The arrays were scanned with an Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Chip data were analyzed with GeneChip Operating Software (GCOS 1.1; Affymetrix) default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 200
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulrik,,Stervbo
| | Sample_contact_email | ulrik.stervbo@gmail.com
| | Sample_contact_laboratory | AG Thiel
| | Sample_contact_institute | BCRT
| | Sample_contact_address | Föhrer Straße 15
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1079nnn/GSM1079349/suppl/GSM1079349_BC_031_CD8+_CD40L-_2_133P.CEL.gz
| | Sample_series_id | GSE44126
| | Sample_data_row_count | 54675
| | |
|
GSM1079350 | GPL570 |
|
CD8+CD40L-, biological rep2
|
PBMC stimulated with PMA/Ionomycin for 6 h
|
tissue: Blood
gender: Male
cell population: CD8+CD45RA-CD40L-
|
Gene expression data of human CD8+CD40L- stimulated with PMA/Ionomycin for 6 h
|
| Sample_geo_accession | GSM1079350
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Feb 06 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | PBMCs were isolated by Ficoll gradient from buffy coat. CD4+ and CD8+ T cells were isolated via MACS, The cells were stimulated with PMA/Ionomycin for 6 h, and subsequently sorted into the CD4+CD45RA-CD40L+, CD8+CD45RA-CD40L-, CD8+CD45RA-CD40L+ populations by FACS-sort
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 3-5 ug total RNA according to the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_hyb_protocol | 15 µg fragmented cRNA was hybridized to the Affymetrix HG-U133Plus2 gene chip in a Hybridization Oven 640, washed and stained in the Fluidics Station 400 (Affymetrix), according to procedure 2 described in the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_scan_protocol | The arrays were scanned with an Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Chip data were analyzed with GeneChip Operating Software (GCOS 1.1; Affymetrix) default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 200
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulrik,,Stervbo
| | Sample_contact_email | ulrik.stervbo@gmail.com
| | Sample_contact_laboratory | AG Thiel
| | Sample_contact_institute | BCRT
| | Sample_contact_address | Föhrer Straße 15
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1079nnn/GSM1079350/suppl/GSM1079350_BC_036_CD8+_CD40L-_3_133P.CEL.gz
| | Sample_series_id | GSE44126
| | Sample_data_row_count | 54675
| | |
|
GSM1079351 | GPL570 |
|
CD8+CD40L-, biological rep3
|
PBMC stimulated with PMA/Ionomycin for 6 h
|
tissue: Blood
gender: Male
cell population: CD8+CD45RA-CD40L-
|
Gene expression data of human CD8+CD40L- stimulated with PMA/Ionomycin for 6 h
|
| Sample_geo_accession | GSM1079351
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Feb 06 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | PBMCs were isolated by Ficoll gradient from buffy coat. CD4+ and CD8+ T cells were isolated via MACS, The cells were stimulated with PMA/Ionomycin for 6 h, and subsequently sorted into the CD4+CD45RA-CD40L+, CD8+CD45RA-CD40L-, CD8+CD45RA-CD40L+ populations by FACS-sort
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 3-5 ug total RNA according to the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_hyb_protocol | 15 µg fragmented cRNA was hybridized to the Affymetrix HG-U133Plus2 gene chip in a Hybridization Oven 640, washed and stained in the Fluidics Station 400 (Affymetrix), according to procedure 2 described in the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_scan_protocol | The arrays were scanned with an Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Chip data were analyzed with GeneChip Operating Software (GCOS 1.1; Affymetrix) default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 200
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulrik,,Stervbo
| | Sample_contact_email | ulrik.stervbo@gmail.com
| | Sample_contact_laboratory | AG Thiel
| | Sample_contact_institute | BCRT
| | Sample_contact_address | Föhrer Straße 15
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1079nnn/GSM1079351/suppl/GSM1079351_BC_077_CD8+_CD40L-_5_133P.CEL.gz
| | Sample_series_id | GSE44126
| | Sample_data_row_count | 54675
| | |
|
GSM1079352 | GPL570 |
|
CD8+CD40L-, biological rep4
|
PBMC stimulated with PMA/Ionomycin for 6 h
|
tissue: Blood
gender: Female
cell population: CD8+CD45RA-CD40L-
|
Gene expression data of human CD8+CD40L- stimulated with PMA/Ionomycin for 6 h
|
| Sample_geo_accession | GSM1079352
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Feb 06 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | PBMCs were isolated by Ficoll gradient from buffy coat. CD4+ and CD8+ T cells were isolated via MACS, The cells were stimulated with PMA/Ionomycin for 6 h, and subsequently sorted into the CD4+CD45RA-CD40L+, CD8+CD45RA-CD40L-, CD8+CD45RA-CD40L+ populations by FACS-sort
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 3-5 ug total RNA according to the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_hyb_protocol | 15 µg fragmented cRNA was hybridized to the Affymetrix HG-U133Plus2 gene chip in a Hybridization Oven 640, washed and stained in the Fluidics Station 400 (Affymetrix), according to procedure 2 described in the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_scan_protocol | The arrays were scanned with an Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Chip data were analyzed with GeneChip Operating Software (GCOS 1.1; Affymetrix) default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 200
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulrik,,Stervbo
| | Sample_contact_email | ulrik.stervbo@gmail.com
| | Sample_contact_laboratory | AG Thiel
| | Sample_contact_institute | BCRT
| | Sample_contact_address | Föhrer Straße 15
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1079nnn/GSM1079352/suppl/GSM1079352_BC_229_CD8+_CD40L-_4_133P.CEL.gz
| | Sample_series_id | GSE44126
| | Sample_data_row_count | 54675
| | |
|
GSM1079353 | GPL570 |
|
CD8+CD40L-, biological rep5
|
PBMC stimulated with PMA/Ionomycin for 6 h
|
tissue: Blood
gender: Male
cell population: CD8+CD45RA-CD40L-
|
Gene expression data of human CD8+CD40L- stimulated with PMA/Ionomycin for 6 h
|
| Sample_geo_accession | GSM1079353
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Feb 06 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | PBMCs were isolated by Ficoll gradient from buffy coat. CD4+ and CD8+ T cells were isolated via MACS, The cells were stimulated with PMA/Ionomycin for 6 h, and subsequently sorted into the CD4+CD45RA-CD40L+, CD8+CD45RA-CD40L-, CD8+CD45RA-CD40L+ populations by FACS-sort
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 3-5 ug total RNA according to the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_hyb_protocol | 15 µg fragmented cRNA was hybridized to the Affymetrix HG-U133Plus2 gene chip in a Hybridization Oven 640, washed and stained in the Fluidics Station 400 (Affymetrix), according to procedure 2 described in the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_scan_protocol | The arrays were scanned with an Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Chip data were analyzed with GeneChip Operating Software (GCOS 1.1; Affymetrix) default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 200
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulrik,,Stervbo
| | Sample_contact_email | ulrik.stervbo@gmail.com
| | Sample_contact_laboratory | AG Thiel
| | Sample_contact_institute | BCRT
| | Sample_contact_address | Föhrer Straße 15
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1079nnn/GSM1079353/suppl/GSM1079353_BC_782_CD8+_CD40L-_1_133P.CEL.gz
| | Sample_series_id | GSE44126
| | Sample_data_row_count | 54675
| | |
|
GSM1079354 | GPL570 |
|
CD8+CD40L+, biological rep1
|
PBMC stimulated with PMA/Ionomycin for 6 h
|
tissue: Blood
gender: Male
cell population: CD8+CD45RA-CD40L+
|
Gene expression data of human CD8+CD40L+ stimulated with PMA/Ionomycin for 6 h
|
| Sample_geo_accession | GSM1079354
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Feb 06 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | PBMCs were isolated by Ficoll gradient from buffy coat. CD4+ and CD8+ T cells were isolated via MACS, The cells were stimulated with PMA/Ionomycin for 6 h, and subsequently sorted into the CD4+CD45RA-CD40L+, CD8+CD45RA-CD40L-, CD8+CD45RA-CD40L+ populations by FACS-sort
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 3-5 ug total RNA according to the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_hyb_protocol | 15 µg fragmented cRNA was hybridized to the Affymetrix HG-U133Plus2 gene chip in a Hybridization Oven 640, washed and stained in the Fluidics Station 400 (Affymetrix), according to procedure 2 described in the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_scan_protocol | The arrays were scanned with an Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Chip data were analyzed with GeneChip Operating Software (GCOS 1.1; Affymetrix) default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 200
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulrik,,Stervbo
| | Sample_contact_email | ulrik.stervbo@gmail.com
| | Sample_contact_laboratory | AG Thiel
| | Sample_contact_institute | BCRT
| | Sample_contact_address | Föhrer Straße 15
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1079nnn/GSM1079354/suppl/GSM1079354_BC_031_CD8+_CD40L+_2_133P.CEL.gz
| | Sample_series_id | GSE44126
| | Sample_data_row_count | 54675
| | |
|
GSM1079355 | GPL570 |
|
CD8+CD40L+, biological rep2
|
PBMC stimulated with PMA/Ionomycin for 6 h
|
tissue: Blood
gender: Male
cell population: CD8+CD45RA-CD40L+
|
Gene expression data of human CD8+CD40L+ stimulated with PMA/Ionomycin for 6 h
|
| Sample_geo_accession | GSM1079355
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Feb 06 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | PBMCs were isolated by Ficoll gradient from buffy coat. CD4+ and CD8+ T cells were isolated via MACS, The cells were stimulated with PMA/Ionomycin for 6 h, and subsequently sorted into the CD4+CD45RA-CD40L+, CD8+CD45RA-CD40L-, CD8+CD45RA-CD40L+ populations by FACS-sort
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 3-5 ug total RNA according to the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_hyb_protocol | 15 µg fragmented cRNA was hybridized to the Affymetrix HG-U133Plus2 gene chip in a Hybridization Oven 640, washed and stained in the Fluidics Station 400 (Affymetrix), according to procedure 2 described in the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_scan_protocol | The arrays were scanned with an Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Chip data were analyzed with GeneChip Operating Software (GCOS 1.1; Affymetrix) default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 200
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulrik,,Stervbo
| | Sample_contact_email | ulrik.stervbo@gmail.com
| | Sample_contact_laboratory | AG Thiel
| | Sample_contact_institute | BCRT
| | Sample_contact_address | Föhrer Straße 15
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1079nnn/GSM1079355/suppl/GSM1079355_BC_036_CD8+_CD40L+_3_133P.CEL.gz
| | Sample_series_id | GSE44126
| | Sample_data_row_count | 54675
| | |
|
GSM1079356 | GPL570 |
|
CD8+CD40L+, biological rep3
|
PBMC stimulated with PMA/Ionomycin for 6 h
|
tissue: Blood
gender: Male
cell population: CD8+CD45RA-CD40L+
|
Gene expression data of human CD8+CD40L+ stimulated with PMA/Ionomycin for 6 h
|
| Sample_geo_accession | GSM1079356
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Feb 06 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | PBMCs were isolated by Ficoll gradient from buffy coat. CD4+ and CD8+ T cells were isolated via MACS, The cells were stimulated with PMA/Ionomycin for 6 h, and subsequently sorted into the CD4+CD45RA-CD40L+, CD8+CD45RA-CD40L-, CD8+CD45RA-CD40L+ populations by FACS-sort
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 3-5 ug total RNA according to the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_hyb_protocol | 15 µg fragmented cRNA was hybridized to the Affymetrix HG-U133Plus2 gene chip in a Hybridization Oven 640, washed and stained in the Fluidics Station 400 (Affymetrix), according to procedure 2 described in the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_scan_protocol | The arrays were scanned with an Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Chip data were analyzed with GeneChip Operating Software (GCOS 1.1; Affymetrix) default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 200
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulrik,,Stervbo
| | Sample_contact_email | ulrik.stervbo@gmail.com
| | Sample_contact_laboratory | AG Thiel
| | Sample_contact_institute | BCRT
| | Sample_contact_address | Föhrer Straße 15
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1079nnn/GSM1079356/suppl/GSM1079356_BC_077_CD8+_CD40L+_5_133P.CEL.gz
| | Sample_series_id | GSE44126
| | Sample_data_row_count | 54675
| | |
|
GSM1079357 | GPL570 |
|
CD8+CD40L+, biological rep4
|
PBMC stimulated with PMA/Ionomycin for 6 h
|
tissue: Blood
gender: Female
cell population: CD8+CD45RA-CD40L+
|
Gene expression data of human CD8+CD40L+ stimulated with PMA/Ionomycin for 6 h
|
| Sample_geo_accession | GSM1079357
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Feb 06 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | PBMCs were isolated by Ficoll gradient from buffy coat. CD4+ and CD8+ T cells were isolated via MACS, The cells were stimulated with PMA/Ionomycin for 6 h, and subsequently sorted into the CD4+CD45RA-CD40L+, CD8+CD45RA-CD40L-, CD8+CD45RA-CD40L+ populations by FACS-sort
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 3-5 ug total RNA according to the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_hyb_protocol | 15 µg fragmented cRNA was hybridized to the Affymetrix HG-U133Plus2 gene chip in a Hybridization Oven 640, washed and stained in the Fluidics Station 400 (Affymetrix), according to procedure 2 described in the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_scan_protocol | The arrays were scanned with an Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Chip data were analyzed with GeneChip Operating Software (GCOS 1.1; Affymetrix) default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 200
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulrik,,Stervbo
| | Sample_contact_email | ulrik.stervbo@gmail.com
| | Sample_contact_laboratory | AG Thiel
| | Sample_contact_institute | BCRT
| | Sample_contact_address | Föhrer Straße 15
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1079nnn/GSM1079357/suppl/GSM1079357_BC_229_CD8+_CD40L+_4_133P.CEL.gz
| | Sample_series_id | GSE44126
| | Sample_data_row_count | 54675
| | |
|
GSM1079358 | GPL570 |
|
CD8+CD40L+, biological rep5
|
PBMC stimulated with PMA/Ionomycin for 6 h
|
tissue: Blood
gender: Male
cell population: CD8+CD45RA-CD40L+
|
Gene expression data of human CD8+CD40L+ stimulated with PMA/Ionomycin for 6 h
|
| Sample_geo_accession | GSM1079358
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Feb 06 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | PBMCs were isolated by Ficoll gradient from buffy coat. CD4+ and CD8+ T cells were isolated via MACS, The cells were stimulated with PMA/Ionomycin for 6 h, and subsequently sorted into the CD4+CD45RA-CD40L+, CD8+CD45RA-CD40L-, CD8+CD45RA-CD40L+ populations by FACS-sort
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 3-5 ug total RNA according to the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_hyb_protocol | 15 µg fragmented cRNA was hybridized to the Affymetrix HG-U133Plus2 gene chip in a Hybridization Oven 640, washed and stained in the Fluidics Station 400 (Affymetrix), according to procedure 2 described in the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)
| | Sample_scan_protocol | The arrays were scanned with an Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Chip data were analyzed with GeneChip Operating Software (GCOS 1.1; Affymetrix) default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 200
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ulrik,,Stervbo
| | Sample_contact_email | ulrik.stervbo@gmail.com
| | Sample_contact_laboratory | AG Thiel
| | Sample_contact_institute | BCRT
| | Sample_contact_address | Föhrer Straße 15
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1079nnn/GSM1079358/suppl/GSM1079358_BC_782_CD8+_CD40L+_1_133P.CEL.gz
| | Sample_series_id | GSE44126
| | Sample_data_row_count | 54675
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
