Search results for the GEO ID: GSE44162  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1079827 | GPL1261 |
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Sarcoma cell line - Dicer Heterozygote -rep1
|
Sarcoma cell line heterozygous for Dicer
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cell type: Sarcoma cell line
strain: C57BL6/J
genotype/variation: Dicer Heterozygote
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| Sample_geo_accession | GSM1079827
| | Sample_status | Public on Apr 10 2013
| | Sample_submission_date | Feb 07 2013
| | Sample_last_update_date | Apr 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Dicer-heterozygous and Dicer KO cells were grown to confluence in T25s and harvested for extraction of total RNA.
| | Sample_growth_protocol_ch1 | Cells were cultured in DMEM supplemented with penicillin/streptomycin, L-glutamine, and 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Mouse 430_2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.0, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.0, Biobase_2.8.0
| | Sample_platform_id | GPL1261
| | Sample_contact_name | A,,Bhutkar
| | Sample_contact_institute | MIT
| | Sample_contact_address | 77 Massachusetts Avenue
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1079nnn/GSM1079827/suppl/GSM1079827_A9121_PA_het.CEL.gz
| | Sample_series_id | GSE44162
| | Sample_series_id | GSE44163
| | Sample_data_row_count | 45037
| | |
|
GSM1079828 | GPL1261 |
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Sarcoma cell line - Dicer Heterozygote -rep2
|
Sarcoma cell line heterozygous for Dicer
|
cell type: Sarcoma cell line
strain: C57BL6/J
genotype/variation: Dicer Heterozygote
|
|
| Sample_geo_accession | GSM1079828
| | Sample_status | Public on Apr 10 2013
| | Sample_submission_date | Feb 07 2013
| | Sample_last_update_date | Apr 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Dicer-heterozygous and Dicer KO cells were grown to confluence in T25s and harvested for extraction of total RNA.
| | Sample_growth_protocol_ch1 | Cells were cultured in DMEM supplemented with penicillin/streptomycin, L-glutamine, and 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Mouse 430_2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.0, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.0, Biobase_2.8.0
| | Sample_platform_id | GPL1261
| | Sample_contact_name | A,,Bhutkar
| | Sample_contact_institute | MIT
| | Sample_contact_address | 77 Massachusetts Avenue
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1079nnn/GSM1079828/suppl/GSM1079828_A9122_2A_het.CEL.gz
| | Sample_series_id | GSE44162
| | Sample_series_id | GSE44163
| | Sample_data_row_count | 45037
| | |
|
GSM1079829 | GPL1261 |
|
Sarcoma cell line - Dicer Heterozygote -rep3
|
Sarcoma cell line heterozygous for Dicer
|
cell type: Sarcoma cell line
strain: C57BL6/J
genotype/variation: Dicer Heterozygote
|
|
| Sample_geo_accession | GSM1079829
| | Sample_status | Public on Apr 10 2013
| | Sample_submission_date | Feb 07 2013
| | Sample_last_update_date | Apr 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Dicer-heterozygous and Dicer KO cells were grown to confluence in T25s and harvested for extraction of total RNA.
| | Sample_growth_protocol_ch1 | Cells were cultured in DMEM supplemented with penicillin/streptomycin, L-glutamine, and 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Mouse 430_2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.0, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.0, Biobase_2.8.0
| | Sample_platform_id | GPL1261
| | Sample_contact_name | A,,Bhutkar
| | Sample_contact_institute | MIT
| | Sample_contact_address | 77 Massachusetts Avenue
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1079nnn/GSM1079829/suppl/GSM1079829_A9125_1.4B_het.CEL.gz
| | Sample_series_id | GSE44162
| | Sample_series_id | GSE44163
| | Sample_data_row_count | 45037
| | |
|
GSM1079830 | GPL1261 |
|
Sarcoma cell line - Dicer Null -rep1
|
Sarcoma cell line Null for Dicer
|
cell type: Sarcoma cell line
strain: C57BL6/J
genotype/variation: Dicer Null
|
|
| Sample_geo_accession | GSM1079830
| | Sample_status | Public on Apr 10 2013
| | Sample_submission_date | Feb 07 2013
| | Sample_last_update_date | Apr 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Dicer-heterozygous and Dicer KO cells were grown to confluence in T25s and harvested for extraction of total RNA.
| | Sample_growth_protocol_ch1 | Cells were cultured in DMEM supplemented with penicillin/streptomycin, L-glutamine, and 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Mouse 430_2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.0, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.0, Biobase_2.8.0
| | Sample_platform_id | GPL1261
| | Sample_contact_name | A,,Bhutkar
| | Sample_contact_institute | MIT
| | Sample_contact_address | 77 Massachusetts Avenue
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1079nnn/GSM1079830/suppl/GSM1079830_A9123_3A_null.CEL.gz
| | Sample_series_id | GSE44162
| | Sample_series_id | GSE44163
| | Sample_data_row_count | 45037
| | |
|
GSM1079831 | GPL1261 |
|
Sarcoma cell line - Dicer Null -rep2
|
Sarcoma cell line Null for Dicer
|
cell type: Sarcoma cell line
strain: C57BL6/J
genotype/variation: Dicer Null
|
|
| Sample_geo_accession | GSM1079831
| | Sample_status | Public on Apr 10 2013
| | Sample_submission_date | Feb 07 2013
| | Sample_last_update_date | Apr 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Dicer-heterozygous and Dicer KO cells were grown to confluence in T25s and harvested for extraction of total RNA.
| | Sample_growth_protocol_ch1 | Cells were cultured in DMEM supplemented with penicillin/streptomycin, L-glutamine, and 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Mouse 430_2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.0, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.0, Biobase_2.8.0
| | Sample_platform_id | GPL1261
| | Sample_contact_name | A,,Bhutkar
| | Sample_contact_institute | MIT
| | Sample_contact_address | 77 Massachusetts Avenue
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1079nnn/GSM1079831/suppl/GSM1079831_A9124_4B_null.CEL.gz
| | Sample_series_id | GSE44162
| | Sample_series_id | GSE44163
| | Sample_data_row_count | 45037
| | |
|
GSM1079832 | GPL1261 |
|
Sarcoma cell line - Dicer Null -rep3
|
Sarcoma cell line Null for Dicer
|
cell type: Sarcoma cell line
strain: C57BL6/J
genotype/variation: Dicer Null
|
|
| Sample_geo_accession | GSM1079832
| | Sample_status | Public on Apr 10 2013
| | Sample_submission_date | Feb 07 2013
| | Sample_last_update_date | Apr 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Dicer-heterozygous and Dicer KO cells were grown to confluence in T25s and harvested for extraction of total RNA.
| | Sample_growth_protocol_ch1 | Cells were cultured in DMEM supplemented with penicillin/streptomycin, L-glutamine, and 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Mouse 430_2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.0, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.0, Biobase_2.8.0
| | Sample_platform_id | GPL1261
| | Sample_contact_name | A,,Bhutkar
| | Sample_contact_institute | MIT
| | Sample_contact_address | 77 Massachusetts Avenue
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02139
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1079nnn/GSM1079832/suppl/GSM1079832_A9126_1.5B_null.CEL.gz
| | Sample_series_id | GSE44162
| | Sample_series_id | GSE44163
| | Sample_data_row_count | 45037
| | |
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