Search results for the GEO ID: GSE44238  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1081136 | GPL570 |
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SCC23/MET/Scrsh
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Scramble shRNA control
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cell line: SCC23/MET
knockdown: scramble shRNA
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Gene expression data in SCC23/MET infected with control virus
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| Sample_geo_accession | GSM1081136
| | Sample_status | Public on May 03 2013
| | Sample_submission_date | Feb 11 2013
| | Sample_last_update_date | May 03 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were grown in humidified 5% CO2 incubator in DMEM supplemented with 10% FBS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy kit (Qiagen) extraction of total RNA was performed according to the manufacture's protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA ( Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human Genome 133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G
| | Sample_data_processing | Bioconductor and R language were used for pre-processing the two hybridizations. The robust multi-array analysis(RMA) was used for bacjground adjustment,normalization and log2 transformation of the signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | xiangming,,ding
| | Sample_contact_email | dingxm@ucla.edu
| | Sample_contact_laboratory | wang
| | Sample_contact_department | dental school
| | Sample_contact_institute | ucla
| | Sample_contact_address | 10833 Le Conte Ave
| | Sample_contact_city | los angeles
| | Sample_contact_state | ca
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1081nnn/GSM1081136/suppl/GSM1081136_SCC23_MET_Scrsh.CEL.gz
| | Sample_series_id | GSE44238
| | Sample_data_row_count | 54675
| | |
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GSM1081137 | GPL570 |
|
SCC23/MET/KDM4Ash
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KDM4A knockdown
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cell line: SCC23/MET
knockdown: KDM4A shRNA
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Gene expression data in SCC23/MET infected with KDM4A knockdown virus
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| Sample_geo_accession | GSM1081137
| | Sample_status | Public on May 03 2013
| | Sample_submission_date | Feb 11 2013
| | Sample_last_update_date | May 03 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were grown in humidified 5% CO2 incubator in DMEM supplemented with 10% FBS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy kit (Qiagen) extraction of total RNA was performed according to the manufacture's protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA ( Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human Genome 133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G
| | Sample_data_processing | Bioconductor and R language were used for pre-processing the two hybridizations. The robust multi-array analysis(RMA) was used for bacjground adjustment,normalization and log2 transformation of the signal intensity
| | Sample_platform_id | GPL570
| | Sample_contact_name | xiangming,,ding
| | Sample_contact_email | dingxm@ucla.edu
| | Sample_contact_laboratory | wang
| | Sample_contact_department | dental school
| | Sample_contact_institute | ucla
| | Sample_contact_address | 10833 Le Conte Ave
| | Sample_contact_city | los angeles
| | Sample_contact_state | ca
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1081nnn/GSM1081137/suppl/GSM1081137_SCC23_MET_KDM4Ash.CEL.gz
| | Sample_series_id | GSE44238
| | Sample_data_row_count | 54675
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