Search results for the GEO ID: GSE44251  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1081335 | GPL1261 |
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undifferentiated_PGK12.1
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undifferentiated (day0) mouse female ES cells PGK12.1
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strain: Derived from blastcysts from a 129 female crossed to a (129 X PGK)F1 male.
cell type: PGK12.1 ES cell line
passage: 14-18
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| Sample_geo_accession | GSM1081335
| | Sample_status | Public on Feb 15 2013
| | Sample_submission_date | Feb 11 2013
| | Sample_last_update_date | Feb 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | ES cells were maintained in standard ES medium with 1000 U/ml leukemia inhibitory factor (LIF) (Millipore) on MEF feeders. ES cells were incubated on 1% gelatin coated dishes for 30min to deplete MEF feeders and transferred to fresh gelatin coated plates for culture for experiments and cell collection. After depletion of MEF feeders, mouse female ES cells were differentiated for 15 days by the EB (embryoid body) differentiation protocol.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared by the Qiagen RNeasy kit with on-column DNaseI digestion
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA.
| | Sample_hyb_protocol | Hybridization and washing were performed according to the standard Affymetrix protocol.
| | Sample_scan_protocol | GeneChips were scanned and processed by the University of Washington Microarray Center.
| | Sample_data_processing | The data were analyzed with the Affymetrix GCOS1.1 software.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Xinxian ,,Deng
| | Sample_contact_email | dengx2@u.washington.edu
| | Sample_contact_laboratory | HSB C526
| | Sample_contact_department | Pathology
| | Sample_contact_institute | University of Washington
| | Sample_contact_address | 1959 NE Pacific St.
| | Sample_contact_city | Seattle
| | Sample_contact_state | WA
| | Sample_contact_zip/postal_code | 98195
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1081nnn/GSM1081335/suppl/GSM1081335_PGK12.1ES.CEL.gz
| | Sample_series_id | GSE30761
| | Sample_series_id | GSE44251
| | Sample_data_row_count | 45101
| | |
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GSM1081336 | GPL1261 |
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15-day-differentiated_PGK12.1
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15-day-differentiated PGK12.1 cells
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strain: Derived from blastcysts from a 129 female crossed to a (129 X PGK)F1 male.
cell type: differentiated PGK12.1 cells
passage: 14-18
|
|
| Sample_geo_accession | GSM1081336
| | Sample_status | Public on Feb 15 2013
| | Sample_submission_date | Feb 11 2013
| | Sample_last_update_date | Feb 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | ES cells were maintained in standard ES medium with 1000 U/ml leukemia inhibitory factor (LIF) (Millipore) on MEF feeders. ES cells were incubated on 1% gelatin coated dishes for 30min to deplete MEF feeders and transferred to fresh gelatin coated plates for culture for experiments and cell collection. After depletion of MEF feeders, mouse female ES cells were differentiated for 15 days by the EB (embryoid body) differentiation protocol.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared by the Qiagen RNeasy kit with on-column DNaseI digestion
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA.
| | Sample_hyb_protocol | Hybridization and washing were performed according to the standard Affymetrix protocol.
| | Sample_scan_protocol | GeneChips were scanned and processed by the University of Washington Microarray Center.
| | Sample_data_processing | The data were analyzed with the Affymetrix GCOS1.1 software.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Xinxian ,,Deng
| | Sample_contact_email | dengx2@u.washington.edu
| | Sample_contact_laboratory | HSB C526
| | Sample_contact_department | Pathology
| | Sample_contact_institute | University of Washington
| | Sample_contact_address | 1959 NE Pacific St.
| | Sample_contact_city | Seattle
| | Sample_contact_state | WA
| | Sample_contact_zip/postal_code | 98195
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1081nnn/GSM1081336/suppl/GSM1081336_PGK12.1d15.CEL.gz
| | Sample_series_id | GSE30761
| | Sample_series_id | GSE44251
| | Sample_data_row_count | 45101
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