Search results for the GEO ID: GSE44335  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1083445 | GPL570 |
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HMLER, Vector, Biological Replicate 1
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Ras-transformed immortalized primary human mammary epithelial cells
|
cell line: HMLER
genotype/variation: control vector
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HMLER cells growing in exponential growth phase.
|
| Sample_geo_accession | GSM1083445
| | Sample_status | Public on Feb 15 2013
| | Sample_submission_date | Feb 14 2013
| | Sample_last_update_date | Feb 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were infected with either pWZL-Blast-FOXC2 or empty vector and selected with 4 ug/ml blasticidin.
| | Sample_growth_protocol_ch1 | HMLER cells are cultured in a 1:1 mix of DME:F12 (supplemented with insulin, hEGF, and hydrocortisone) and MEGM.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Qiagen Rneasy Mini kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | We performed an in vitro transcription reaction to produce biotin-labeled cRNA from the cDNA.
| | Sample_hyb_protocol | A hybridization cocktail was prepared that contains the fragmented cRNA, probe array controls, BSA, and herring sperm DNA. The cRNA is hybridized to the oligonucleotide probes on the array for 16 hours at 45C.
| | Sample_scan_protocol | Immediately following the hybridization, the hybridized probe arrays undergo an automated washing and staining protocol on the fluidic station and are then scanned on the GeneChip Scanner 3000 7G.
| | Sample_data_processing | RMA preprocessing using the Bioconductor toolkit.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffrey,,Chang
| | Sample_contact_email | jeffrey.t.chang@uth.tmc.edu
| | Sample_contact_institute | UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
| | Sample_contact_address | 6431 Fannin St
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1083nnn/GSM1083445/suppl/GSM1083445_HMLER-vector_1.CEL.gz
| | Sample_series_id | GSE44335
| | Sample_data_row_count | 54675
| | |
|
GSM1083446 | GPL570 |
|
HMLER, Vector, Biological Replicate 2
|
Ras-transformed immortalized primary human mammary epithelial cells
|
cell line: HMLER
genotype/variation: control vector
|
HMLER cells growing in exponential growth phase.
|
| Sample_geo_accession | GSM1083446
| | Sample_status | Public on Feb 15 2013
| | Sample_submission_date | Feb 14 2013
| | Sample_last_update_date | Feb 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were infected with either pWZL-Blast-FOXC2 or empty vector and selected with 4 ug/ml blasticidin.
| | Sample_growth_protocol_ch1 | HMLER cells are cultured in a 1:1 mix of DME:F12 (supplemented with insulin, hEGF, and hydrocortisone) and MEGM.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Qiagen Rneasy Mini kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | We performed an in vitro transcription reaction to produce biotin-labeled cRNA from the cDNA.
| | Sample_hyb_protocol | A hybridization cocktail was prepared that contains the fragmented cRNA, probe array controls, BSA, and herring sperm DNA. The cRNA is hybridized to the oligonucleotide probes on the array for 16 hours at 45C.
| | Sample_scan_protocol | Immediately following the hybridization, the hybridized probe arrays undergo an automated washing and staining protocol on the fluidic station and are then scanned on the GeneChip Scanner 3000 7G.
| | Sample_data_processing | RMA preprocessing using the Bioconductor toolkit.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffrey,,Chang
| | Sample_contact_email | jeffrey.t.chang@uth.tmc.edu
| | Sample_contact_institute | UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
| | Sample_contact_address | 6431 Fannin St
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1083nnn/GSM1083446/suppl/GSM1083446_HMLER-vector_2.CEL.gz
| | Sample_series_id | GSE44335
| | Sample_data_row_count | 54675
| | |
|
GSM1083447 | GPL570 |
|
HMLER, Vector, Biological Replicate 3
|
Ras-transformed immortalized primary human mammary epithelial cells
|
cell line: HMLER
genotype/variation: control vector
|
HMLER cells growing in exponential growth phase.
|
| Sample_geo_accession | GSM1083447
| | Sample_status | Public on Feb 15 2013
| | Sample_submission_date | Feb 14 2013
| | Sample_last_update_date | Feb 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were infected with either pWZL-Blast-FOXC2 or empty vector and selected with 4 ug/ml blasticidin.
| | Sample_growth_protocol_ch1 | HMLER cells are cultured in a 1:1 mix of DME:F12 (supplemented with insulin, hEGF, and hydrocortisone) and MEGM.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Qiagen Rneasy Mini kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | We performed an in vitro transcription reaction to produce biotin-labeled cRNA from the cDNA.
| | Sample_hyb_protocol | A hybridization cocktail was prepared that contains the fragmented cRNA, probe array controls, BSA, and herring sperm DNA. The cRNA is hybridized to the oligonucleotide probes on the array for 16 hours at 45C.
| | Sample_scan_protocol | Immediately following the hybridization, the hybridized probe arrays undergo an automated washing and staining protocol on the fluidic station and are then scanned on the GeneChip Scanner 3000 7G.
| | Sample_data_processing | RMA preprocessing using the Bioconductor toolkit.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffrey,,Chang
| | Sample_contact_email | jeffrey.t.chang@uth.tmc.edu
| | Sample_contact_institute | UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
| | Sample_contact_address | 6431 Fannin St
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1083nnn/GSM1083447/suppl/GSM1083447_HMLER-vector_3.CEL.gz
| | Sample_series_id | GSE44335
| | Sample_data_row_count | 54675
| | |
|
GSM1083448 | GPL570 |
|
HMLER, FOXC2, Biological Replicate 1
|
Ras-transformed immortalized primary human mammary epithelial cells expressing FOXC2
|
cell line: HMLER
genotype/variation: retroviral vector expressing FOXC2
|
HMLER cells growing in exponential growth phase.
|
| Sample_geo_accession | GSM1083448
| | Sample_status | Public on Feb 15 2013
| | Sample_submission_date | Feb 14 2013
| | Sample_last_update_date | Feb 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were infected with either pWZL-Blast-FOXC2 or empty vector and selected with 4 ug/ml blasticidin.
| | Sample_growth_protocol_ch1 | HMLER cells are cultured in a 1:1 mix of DME:F12 (supplemented with insulin, hEGF, and hydrocortisone) and MEGM.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Qiagen Rneasy Mini kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | We performed an in vitro transcription reaction to produce biotin-labeled cRNA from the cDNA.
| | Sample_hyb_protocol | A hybridization cocktail was prepared that contains the fragmented cRNA, probe array controls, BSA, and herring sperm DNA. The cRNA is hybridized to the oligonucleotide probes on the array for 16 hours at 45C.
| | Sample_scan_protocol | Immediately following the hybridization, the hybridized probe arrays undergo an automated washing and staining protocol on the fluidic station and are then scanned on the GeneChip Scanner 3000 7G.
| | Sample_data_processing | RMA preprocessing using the Bioconductor toolkit.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffrey,,Chang
| | Sample_contact_email | jeffrey.t.chang@uth.tmc.edu
| | Sample_contact_institute | UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
| | Sample_contact_address | 6431 Fannin St
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1083nnn/GSM1083448/suppl/GSM1083448_HMLER-FOXC2_1.CEL.gz
| | Sample_series_id | GSE44335
| | Sample_data_row_count | 54675
| | |
|
GSM1083449 | GPL570 |
|
HMLER, FOXC2, Biological Replicate 2
|
Ras-transformed immortalized primary human mammary epithelial cells expressing FOXC2
|
cell line: HMLER
genotype/variation: retroviral vector expressing FOXC2
|
HMLER cells growing in exponential growth phase.
|
| Sample_geo_accession | GSM1083449
| | Sample_status | Public on Feb 15 2013
| | Sample_submission_date | Feb 14 2013
| | Sample_last_update_date | Feb 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were infected with either pWZL-Blast-FOXC2 or empty vector and selected with 4 ug/ml blasticidin.
| | Sample_growth_protocol_ch1 | HMLER cells are cultured in a 1:1 mix of DME:F12 (supplemented with insulin, hEGF, and hydrocortisone) and MEGM.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Qiagen Rneasy Mini kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | We performed an in vitro transcription reaction to produce biotin-labeled cRNA from the cDNA.
| | Sample_hyb_protocol | A hybridization cocktail was prepared that contains the fragmented cRNA, probe array controls, BSA, and herring sperm DNA. The cRNA is hybridized to the oligonucleotide probes on the array for 16 hours at 45C.
| | Sample_scan_protocol | Immediately following the hybridization, the hybridized probe arrays undergo an automated washing and staining protocol on the fluidic station and are then scanned on the GeneChip Scanner 3000 7G.
| | Sample_data_processing | RMA preprocessing using the Bioconductor toolkit.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffrey,,Chang
| | Sample_contact_email | jeffrey.t.chang@uth.tmc.edu
| | Sample_contact_institute | UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
| | Sample_contact_address | 6431 Fannin St
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1083nnn/GSM1083449/suppl/GSM1083449_HMLER-FOXC2_2.CEL.gz
| | Sample_series_id | GSE44335
| | Sample_data_row_count | 54675
| | |
|
GSM1083450 | GPL570 |
|
HMLER, FOXC2, Biological Replicate 3
|
Ras-transformed immortalized primary human mammary epithelial cells expressing FOXC2
|
cell line: HMLER
genotype/variation: retroviral vector expressing FOXC2
|
HMLER cells growing in exponential growth phase.
|
| Sample_geo_accession | GSM1083450
| | Sample_status | Public on Feb 15 2013
| | Sample_submission_date | Feb 14 2013
| | Sample_last_update_date | Feb 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were infected with either pWZL-Blast-FOXC2 or empty vector and selected with 4 ug/ml blasticidin.
| | Sample_growth_protocol_ch1 | HMLER cells are cultured in a 1:1 mix of DME:F12 (supplemented with insulin, hEGF, and hydrocortisone) and MEGM.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the Qiagen Rneasy Mini kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | We performed an in vitro transcription reaction to produce biotin-labeled cRNA from the cDNA.
| | Sample_hyb_protocol | A hybridization cocktail was prepared that contains the fragmented cRNA, probe array controls, BSA, and herring sperm DNA. The cRNA is hybridized to the oligonucleotide probes on the array for 16 hours at 45C.
| | Sample_scan_protocol | Immediately following the hybridization, the hybridized probe arrays undergo an automated washing and staining protocol on the fluidic station and are then scanned on the GeneChip Scanner 3000 7G.
| | Sample_data_processing | RMA preprocessing using the Bioconductor toolkit.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffrey,,Chang
| | Sample_contact_email | jeffrey.t.chang@uth.tmc.edu
| | Sample_contact_institute | UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
| | Sample_contact_address | 6431 Fannin St
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1083nnn/GSM1083450/suppl/GSM1083450_HMLER-FOXC2_3.CEL.gz
| | Sample_series_id | GSE44335
| | Sample_data_row_count | 54675
| | |
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