Search results for the GEO ID: GSE44355  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1083870 | GPL1261 |
|
lymphoma_5783_nt
|
Primary lymphoma cells, isolated from lymph nodes of Emu-myc,Suv39h1-/- mice and retrovirally transduced by Bcl2, untreated
|
genotype: Emu-myc;Suv39h1-/-
transduction: Bcl2
cell type: primary lymphoma cells from lymph nodes
treatment: none
|
Primary lymphoma cells isolated from lymph nodes of Emu-myc; Suv39h1-/- mice with palpable lymphadenopathy.
|
| Sample_geo_accession | GSM1083870
| | Sample_status | Public on Jul 04 2013
| | Sample_submission_date | Feb 15 2013
| | Sample_last_update_date | Jul 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Freshly isolated lymphoma cells were retrovirally transduced by Bcl2 (MSCVpuro) and selected with Puromycin for 48h. These Myc;Bcl2 cells were either left untreated or treated in vitro with 0.05µg/ml Adriamycin for 5 days.
| | Sample_growth_protocol_ch1 | Primary Emu-myc;Suv39h1-/- lymphoma cells were cultivated in B-cell medium (DMEM and IMDM in 1:1 ratio, 10%FBS, 4mM L-glutamine, 25µM beta-mercaptoethanol, 100U/ml penicilin/streptamycin) on gamma-irradiated (20Gy) NIH 3T3 cells as feeder.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The total RNA from untreated or 5 days ADR treated Myc;Bcl2 lymphomas was prepared using an RNeasy Mini kit (QIAGEN, Valencia, California) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol (One Cycle Target labeling protocol) from 3 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on MOE430 2.0 Arrays (Affymetrix). GeneChips were washed and stained with standard protocols in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Scanner G3000.
| | Sample_data_processing | Affymetrix CEL files were imported into Partek Genomic Suite Software (Version 6.5, Partek Inc., St. Louis, USA) and processed by the implemented RMA workflow (median polish probe set summarization, RMA background correction, quantile normalization).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Maja,,Milanovic
| | Sample_contact_email | maja.milanovic@charite.de
| | Sample_contact_laboratory | Clemens Schmitt
| | Sample_contact_department | Hematology and Oncology
| | Sample_contact_institute | Charite University Medicine, Berlin
| | Sample_contact_address | Augustenburgerplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1083nnn/GSM1083870/suppl/GSM1083870_M1.CEL.gz
| | Sample_series_id | GSE44355
| | Sample_data_row_count | 45101
| | |
|
GSM1083871 | GPL1261 |
|
lymphoma_5783_ADR
|
Primary lymphoma cells, isolated from lymph nodes of Emu-myc,Suv39h1-/- mice and retrovirally transduced by Bcl2, ADR treated
|
genotype: Emu-myc;Suv39h1-/-
transduction: Bcl2
cell type: primary lymphoma cells from lymph nodes
treatment: Adriamycin (ADR)
|
Primary lymphoma cells isolated from lymph nodes of Emu-myc; Suv39h1-/- mice with palpable lymphadenopathy.
|
| Sample_geo_accession | GSM1083871
| | Sample_status | Public on Jul 04 2013
| | Sample_submission_date | Feb 15 2013
| | Sample_last_update_date | Jul 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Freshly isolated lymphoma cells were retrovirally transduced by Bcl2 (MSCVpuro) and selected with Puromycin for 48h. These Myc;Bcl2 cells were either left untreated or treated in vitro with 0.05µg/ml Adriamycin for 5 days.
| | Sample_growth_protocol_ch1 | Primary Emu-myc;Suv39h1-/- lymphoma cells were cultivated in B-cell medium (DMEM and IMDM in 1:1 ratio, 10%FBS, 4mM L-glutamine, 25µM beta-mercaptoethanol, 100U/ml penicilin/streptamycin) on gamma-irradiated (20Gy) NIH 3T3 cells as feeder.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The total RNA from untreated or 5 days ADR treated Myc;Bcl2 lymphomas was prepared using an RNeasy Mini kit (QIAGEN, Valencia, California) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol (One Cycle Target labeling protocol) from 3 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on MOE430 2.0 Arrays (Affymetrix). GeneChips were washed and stained with standard protocols in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Scanner G3000.
| | Sample_data_processing | Affymetrix CEL files were imported into Partek Genomic Suite Software (Version 6.5, Partek Inc., St. Louis, USA) and processed by the implemented RMA workflow (median polish probe set summarization, RMA background correction, quantile normalization).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Maja,,Milanovic
| | Sample_contact_email | maja.milanovic@charite.de
| | Sample_contact_laboratory | Clemens Schmitt
| | Sample_contact_department | Hematology and Oncology
| | Sample_contact_institute | Charite University Medicine, Berlin
| | Sample_contact_address | Augustenburgerplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1083nnn/GSM1083871/suppl/GSM1083871_M2.CEL.gz
| | Sample_series_id | GSE44355
| | Sample_data_row_count | 45101
| | |
|
GSM1083872 | GPL1261 |
|
lymphoma_7890_nt
|
Primary lymphoma cells, isolated from lymph nodes of Emu-myc,Suv39h1-/- mice and retrovirally transduced by Bcl2, untreated
|
genotype: Emu-myc;Suv39h1-/-
transduction: Bcl2
cell type: primary lymphoma cells from lymph nodes
treatment: none
|
Primary lymphoma cells isolated from lymph nodes of Emu-myc; Suv39h1-/- mice with palpable lymphadenopathy.
|
| Sample_geo_accession | GSM1083872
| | Sample_status | Public on Jul 04 2013
| | Sample_submission_date | Feb 15 2013
| | Sample_last_update_date | Jul 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Freshly isolated lymphoma cells were retrovirally transduced by Bcl2 (MSCVpuro) and selected with Puromycin for 48h. These Myc;Bcl2 cells were either left untreated or treated in vitro with 0.05µg/ml Adriamycin for 5 days.
| | Sample_growth_protocol_ch1 | Primary Emu-myc;Suv39h1-/- lymphoma cells were cultivated in B-cell medium (DMEM and IMDM in 1:1 ratio, 10%FBS, 4mM L-glutamine, 25µM beta-mercaptoethanol, 100U/ml penicilin/streptamycin) on gamma-irradiated (20Gy) NIH 3T3 cells as feeder.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The total RNA from untreated or 5 days ADR treated Myc;Bcl2 lymphomas was prepared using an RNeasy Mini kit (QIAGEN, Valencia, California) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol (One Cycle Target labeling protocol) from 3 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on MOE430 2.0 Arrays (Affymetrix). GeneChips were washed and stained with standard protocols in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Scanner G3000.
| | Sample_data_processing | Affymetrix CEL files were imported into Partek Genomic Suite Software (Version 6.5, Partek Inc., St. Louis, USA) and processed by the implemented RMA workflow (median polish probe set summarization, RMA background correction, quantile normalization).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Maja,,Milanovic
| | Sample_contact_email | maja.milanovic@charite.de
| | Sample_contact_laboratory | Clemens Schmitt
| | Sample_contact_department | Hematology and Oncology
| | Sample_contact_institute | Charite University Medicine, Berlin
| | Sample_contact_address | Augustenburgerplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1083nnn/GSM1083872/suppl/GSM1083872_M3.CEL.gz
| | Sample_series_id | GSE44355
| | Sample_data_row_count | 45101
| | |
|
GSM1083873 | GPL1261 |
|
lymphoma_7890_ADR
|
Primary lymphoma cells, isolated from lymph nodes of Emu-myc,Suv39h1-/- mice and retrovirally transduced by Bcl2, ADR treated
|
genotype: Emu-myc;Suv39h1-/-
transduction: Bcl2
cell type: primary lymphoma cells from lymph nodes
treatment: Adriamycin (ADR)
|
Primary lymphoma cells isolated from lymph nodes of Emu-myc; Suv39h1-/- mice with palpable lymphadenopathy.
|
| Sample_geo_accession | GSM1083873
| | Sample_status | Public on Jul 04 2013
| | Sample_submission_date | Feb 15 2013
| | Sample_last_update_date | Jul 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Freshly isolated lymphoma cells were retrovirally transduced by Bcl2 (MSCVpuro) and selected with Puromycin for 48h. These Myc;Bcl2 cells were either left untreated or treated in vitro with 0.05µg/ml Adriamycin for 5 days.
| | Sample_growth_protocol_ch1 | Primary Emu-myc;Suv39h1-/- lymphoma cells were cultivated in B-cell medium (DMEM and IMDM in 1:1 ratio, 10%FBS, 4mM L-glutamine, 25µM beta-mercaptoethanol, 100U/ml penicilin/streptamycin) on gamma-irradiated (20Gy) NIH 3T3 cells as feeder.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The total RNA from untreated or 5 days ADR treated Myc;Bcl2 lymphomas was prepared using an RNeasy Mini kit (QIAGEN, Valencia, California) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol (One Cycle Target labeling protocol) from 3 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on MOE430 2.0 Arrays (Affymetrix). GeneChips were washed and stained with standard protocols in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Scanner G3000.
| | Sample_data_processing | Affymetrix CEL files were imported into Partek Genomic Suite Software (Version 6.5, Partek Inc., St. Louis, USA) and processed by the implemented RMA workflow (median polish probe set summarization, RMA background correction, quantile normalization).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Maja,,Milanovic
| | Sample_contact_email | maja.milanovic@charite.de
| | Sample_contact_laboratory | Clemens Schmitt
| | Sample_contact_department | Hematology and Oncology
| | Sample_contact_institute | Charite University Medicine, Berlin
| | Sample_contact_address | Augustenburgerplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1083nnn/GSM1083873/suppl/GSM1083873_M4.CEL.gz
| | Sample_series_id | GSE44355
| | Sample_data_row_count | 45101
| | |
|
GSM1083874 | GPL1261 |
|
lymphoma_9250_nt
|
Primary lymphoma cells, isolated from lymph nodes of Emu-myc,Suv39h1-/- mice and retrovirally transduced by Bcl2, untreated
|
genotype: Emu-myc;Suv39h1-/-
transduction: Bcl2
cell type: primary lymphoma cells from lymph nodes
treatment: none
|
Primary lymphoma cells isolated from lymph nodes of Emu-myc; Suv39h1-/- mice with palpable lymphadenopathy.
|
| Sample_geo_accession | GSM1083874
| | Sample_status | Public on Jul 04 2013
| | Sample_submission_date | Feb 15 2013
| | Sample_last_update_date | Jul 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Freshly isolated lymphoma cells were retrovirally transduced by Bcl2 (MSCVpuro) and selected with Puromycin for 48h. These Myc;Bcl2 cells were either left untreated or treated in vitro with 0.05µg/ml Adriamycin for 5 days.
| | Sample_growth_protocol_ch1 | Primary Emu-myc;Suv39h1-/- lymphoma cells were cultivated in B-cell medium (DMEM and IMDM in 1:1 ratio, 10%FBS, 4mM L-glutamine, 25µM beta-mercaptoethanol, 100U/ml penicilin/streptamycin) on gamma-irradiated (20Gy) NIH 3T3 cells as feeder.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The total RNA from untreated or 5 days ADR treated Myc;Bcl2 lymphomas was prepared using an RNeasy Mini kit (QIAGEN, Valencia, California) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol (One Cycle Target labeling protocol) from 3 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on MOE430 2.0 Arrays (Affymetrix). GeneChips were washed and stained with standard protocols in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Scanner G3000.
| | Sample_data_processing | Affymetrix CEL files were imported into Partek Genomic Suite Software (Version 6.5, Partek Inc., St. Louis, USA) and processed by the implemented RMA workflow (median polish probe set summarization, RMA background correction, quantile normalization).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Maja,,Milanovic
| | Sample_contact_email | maja.milanovic@charite.de
| | Sample_contact_laboratory | Clemens Schmitt
| | Sample_contact_department | Hematology and Oncology
| | Sample_contact_institute | Charite University Medicine, Berlin
| | Sample_contact_address | Augustenburgerplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1083nnn/GSM1083874/suppl/GSM1083874_M5.CEL.gz
| | Sample_series_id | GSE44355
| | Sample_data_row_count | 45101
| | |
|
GSM1083875 | GPL1261 |
|
lymphoma_9250_ADR
|
Primary lymphoma cells, isolated from lymph nodes of Emu-myc,Suv39h1-/- mice and retrovirally transduced by Bcl2, ADR treated
|
genotype: Emu-myc;Suv39h1-/-
transduction: Bcl2
cell type: primary lymphoma cells from lymph nodes
treatment: Adriamycin (ADR)
|
Primary lymphoma cells isolated from lymph nodes of Emu-myc; Suv39h1-/- mice with palpable lymphadenopathy.
|
| Sample_geo_accession | GSM1083875
| | Sample_status | Public on Jul 04 2013
| | Sample_submission_date | Feb 15 2013
| | Sample_last_update_date | Jul 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Freshly isolated lymphoma cells were retrovirally transduced by Bcl2 (MSCVpuro) and selected with Puromycin for 48h. These Myc;Bcl2 cells were either left untreated or treated in vitro with 0.05µg/ml Adriamycin for 5 days.
| | Sample_growth_protocol_ch1 | Primary Emu-myc;Suv39h1-/- lymphoma cells were cultivated in B-cell medium (DMEM and IMDM in 1:1 ratio, 10%FBS, 4mM L-glutamine, 25µM beta-mercaptoethanol, 100U/ml penicilin/streptamycin) on gamma-irradiated (20Gy) NIH 3T3 cells as feeder.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The total RNA from untreated or 5 days ADR treated Myc;Bcl2 lymphomas was prepared using an RNeasy Mini kit (QIAGEN, Valencia, California) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol (One Cycle Target labeling protocol) from 3 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on MOE430 2.0 Arrays (Affymetrix). GeneChips were washed and stained with standard protocols in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Scanner G3000.
| | Sample_data_processing | Affymetrix CEL files were imported into Partek Genomic Suite Software (Version 6.5, Partek Inc., St. Louis, USA) and processed by the implemented RMA workflow (median polish probe set summarization, RMA background correction, quantile normalization).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Maja,,Milanovic
| | Sample_contact_email | maja.milanovic@charite.de
| | Sample_contact_laboratory | Clemens Schmitt
| | Sample_contact_department | Hematology and Oncology
| | Sample_contact_institute | Charite University Medicine, Berlin
| | Sample_contact_address | Augustenburgerplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1083nnn/GSM1083875/suppl/GSM1083875_M6.CEL.gz
| | Sample_series_id | GSE44355
| | Sample_data_row_count | 45101
| | |
|
GSM1083876 | GPL1261 |
|
lymphoma_1032_nt
|
Primary lymphoma cells, isolated from lymph nodes of Emu-myc,Suv39h1-/- mice and retrovirally transduced by Bcl2, untreated
|
genotype: Emu-myc;Suv39h1-/-
transduction: Bcl2
cell type: primary lymphoma cells from lymph nodes
treatment: none
|
Primary lymphoma cells isolated from lymph nodes of Emu-myc; Suv39h1-/- mice with palpable lymphadenopathy.
|
| Sample_geo_accession | GSM1083876
| | Sample_status | Public on Jul 04 2013
| | Sample_submission_date | Feb 15 2013
| | Sample_last_update_date | Jul 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Freshly isolated lymphoma cells were retrovirally transduced by Bcl2 (MSCVpuro) and selected with Puromycin for 48h. These Myc;Bcl2 cells were either left untreated or treated in vitro with 0.05µg/ml Adriamycin for 5 days.
| | Sample_growth_protocol_ch1 | Primary Emu-myc;Suv39h1-/- lymphoma cells were cultivated in B-cell medium (DMEM and IMDM in 1:1 ratio, 10%FBS, 4mM L-glutamine, 25µM beta-mercaptoethanol, 100U/ml penicilin/streptamycin) on gamma-irradiated (20Gy) NIH 3T3 cells as feeder.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The total RNA from untreated or 5 days ADR treated Myc;Bcl2 lymphomas was prepared using an RNeasy Mini kit (QIAGEN, Valencia, California) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol (One Cycle Target labeling protocol) from 3 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on MOE430 2.0 Arrays (Affymetrix). GeneChips were washed and stained with standard protocols in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Scanner G3000.
| | Sample_data_processing | Affymetrix CEL files were imported into Partek Genomic Suite Software (Version 6.5, Partek Inc., St. Louis, USA) and processed by the implemented RMA workflow (median polish probe set summarization, RMA background correction, quantile normalization).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Maja,,Milanovic
| | Sample_contact_email | maja.milanovic@charite.de
| | Sample_contact_laboratory | Clemens Schmitt
| | Sample_contact_department | Hematology and Oncology
| | Sample_contact_institute | Charite University Medicine, Berlin
| | Sample_contact_address | Augustenburgerplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1083nnn/GSM1083876/suppl/GSM1083876_M7.CEL.gz
| | Sample_series_id | GSE44355
| | Sample_data_row_count | 45101
| | |
|
GSM1083877 | GPL1261 |
|
lymphoma_1032_ADR
|
Primary lymphoma cells, isolated from lymph nodes of Emu-myc,Suv39h1-/- mice and retrovirally transduced by Bcl2, ADR treated
|
genotype: Emu-myc;Suv39h1-/-
transduction: Bcl2
cell type: primary lymphoma cells from lymph nodes
treatment: Adriamycin (ADR)
|
Primary lymphoma cells isolated from lymph nodes of Emu-myc; Suv39h1-/- mice with palpable lymphadenopathy.
|
| Sample_geo_accession | GSM1083877
| | Sample_status | Public on Jul 04 2013
| | Sample_submission_date | Feb 15 2013
| | Sample_last_update_date | Jul 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Freshly isolated lymphoma cells were retrovirally transduced by Bcl2 (MSCVpuro) and selected with Puromycin for 48h. These Myc;Bcl2 cells were either left untreated or treated in vitro with 0.05µg/ml Adriamycin for 5 days.
| | Sample_growth_protocol_ch1 | Primary Emu-myc;Suv39h1-/- lymphoma cells were cultivated in B-cell medium (DMEM and IMDM in 1:1 ratio, 10%FBS, 4mM L-glutamine, 25µM beta-mercaptoethanol, 100U/ml penicilin/streptamycin) on gamma-irradiated (20Gy) NIH 3T3 cells as feeder.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The total RNA from untreated or 5 days ADR treated Myc;Bcl2 lymphomas was prepared using an RNeasy Mini kit (QIAGEN, Valencia, California) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol (One Cycle Target labeling protocol) from 3 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on MOE430 2.0 Arrays (Affymetrix). GeneChips were washed and stained with standard protocols in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Scanner G3000.
| | Sample_data_processing | Affymetrix CEL files were imported into Partek Genomic Suite Software (Version 6.5, Partek Inc., St. Louis, USA) and processed by the implemented RMA workflow (median polish probe set summarization, RMA background correction, quantile normalization).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Maja,,Milanovic
| | Sample_contact_email | maja.milanovic@charite.de
| | Sample_contact_laboratory | Clemens Schmitt
| | Sample_contact_department | Hematology and Oncology
| | Sample_contact_institute | Charite University Medicine, Berlin
| | Sample_contact_address | Augustenburgerplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1083nnn/GSM1083877/suppl/GSM1083877_M8.CEL.gz
| | Sample_series_id | GSE44355
| | Sample_data_row_count | 45101
| | |
|
GSM1083878 | GPL1261 |
|
lymphoma_4936_nt
|
Primary lymphoma cells, isolated from lymph nodes of Emu-myc,Suv39h1-/- mice and retrovirally transduced by Bcl2, untreated
|
genotype: Emu-myc;Suv39h1-/-
transduction: Bcl2
cell type: primary lymphoma cells from lymph nodes
treatment: none
|
Primary lymphoma cells isolated from lymph nodes of Emu-myc; Suv39h1-/- mice with palpable lymphadenopathy.
|
| Sample_geo_accession | GSM1083878
| | Sample_status | Public on Jul 04 2013
| | Sample_submission_date | Feb 15 2013
| | Sample_last_update_date | Jul 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Freshly isolated lymphoma cells were retrovirally transduced by Bcl2 (MSCVpuro) and selected with Puromycin for 48h. These Myc;Bcl2 cells were either left untreated or treated in vitro with 0.05µg/ml Adriamycin for 5 days.
| | Sample_growth_protocol_ch1 | Primary Emu-myc;Suv39h1-/- lymphoma cells were cultivated in B-cell medium (DMEM and IMDM in 1:1 ratio, 10%FBS, 4mM L-glutamine, 25µM beta-mercaptoethanol, 100U/ml penicilin/streptamycin) on gamma-irradiated (20Gy) NIH 3T3 cells as feeder.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The total RNA from untreated or 5 days ADR treated Myc;Bcl2 lymphomas was prepared using an RNeasy Mini kit (QIAGEN, Valencia, California) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol (One Cycle Target labeling protocol) from 3 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on MOE430 2.0 Arrays (Affymetrix). GeneChips were washed and stained with standard protocols in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Scanner G3000.
| | Sample_data_processing | Affymetrix CEL files were imported into Partek Genomic Suite Software (Version 6.5, Partek Inc., St. Louis, USA) and processed by the implemented RMA workflow (median polish probe set summarization, RMA background correction, quantile normalization).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Maja,,Milanovic
| | Sample_contact_email | maja.milanovic@charite.de
| | Sample_contact_laboratory | Clemens Schmitt
| | Sample_contact_department | Hematology and Oncology
| | Sample_contact_institute | Charite University Medicine, Berlin
| | Sample_contact_address | Augustenburgerplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1083nnn/GSM1083878/suppl/GSM1083878_M9.CEL.gz
| | Sample_series_id | GSE44355
| | Sample_data_row_count | 45101
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GSM1083879 | GPL1261 |
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lymphoma_4936_ADR
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Primary lymphoma cells, isolated from lymph nodes of Emu-myc,Suv39h1-/- mice and retrovirally transduced by Bcl2, ADR treated
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genotype: Emu-myc;Suv39h1-/-
transduction: Bcl2
cell type: primary lymphoma cells from lymph nodes
treatment: Adriamycin (ADR)
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Primary lymphoma cells isolated from lymph nodes of Emu-myc; Suv39h1-/- mice with palpable lymphadenopathy.
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| Sample_geo_accession | GSM1083879
| | Sample_status | Public on Jul 04 2013
| | Sample_submission_date | Feb 15 2013
| | Sample_last_update_date | Jul 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Freshly isolated lymphoma cells were retrovirally transduced by Bcl2 (MSCVpuro) and selected with Puromycin for 48h. These Myc;Bcl2 cells were either left untreated or treated in vitro with 0.05µg/ml Adriamycin for 5 days.
| | Sample_growth_protocol_ch1 | Primary Emu-myc;Suv39h1-/- lymphoma cells were cultivated in B-cell medium (DMEM and IMDM in 1:1 ratio, 10%FBS, 4mM L-glutamine, 25µM beta-mercaptoethanol, 100U/ml penicilin/streptamycin) on gamma-irradiated (20Gy) NIH 3T3 cells as feeder.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The total RNA from untreated or 5 days ADR treated Myc;Bcl2 lymphomas was prepared using an RNeasy Mini kit (QIAGEN, Valencia, California) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol (One Cycle Target labeling protocol) from 3 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on MOE430 2.0 Arrays (Affymetrix). GeneChips were washed and stained with standard protocols in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Scanner G3000.
| | Sample_data_processing | Affymetrix CEL files were imported into Partek Genomic Suite Software (Version 6.5, Partek Inc., St. Louis, USA) and processed by the implemented RMA workflow (median polish probe set summarization, RMA background correction, quantile normalization).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Maja,,Milanovic
| | Sample_contact_email | maja.milanovic@charite.de
| | Sample_contact_laboratory | Clemens Schmitt
| | Sample_contact_department | Hematology and Oncology
| | Sample_contact_institute | Charite University Medicine, Berlin
| | Sample_contact_address | Augustenburgerplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13353
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1083nnn/GSM1083879/suppl/GSM1083879_M10.CEL.gz
| | Sample_series_id | GSE44355
| | Sample_data_row_count | 45101
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