Search results for the GEO ID: GSE44537  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1086183 | GPL570 |
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SKNAS_Monolayer_rep_1
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Neuroblastoma cell line
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cell origin: Neuroblastoma
culture condition: Monolayer
pretreatment: None
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| Sample_geo_accession | GSM1086183
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Feb 21 2013
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | SKNAS iCSC was first treated with 5AdC (2.5 uM) for five days as monolayer culture and then transferred to the serum free medium to form spheroid culture.
| | Sample_growth_protocol_ch1 | SKNAS Monolayer was grown as monolayer culture. SKNAS Sphere was grown as spheroid culture in a serum free medium. SKNAS iCSC was grown as spheroid culture in a serum free medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen RNeasy kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated amplified RNA (aRNA) was prepared from 200 ng total RNA using the standard protocol of the Ambion MessageAmp Premier Kit.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133_P_2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | Probe-level data in CEL files was normalized using GCRMA method implemented in Partek Genomics Suite to get gene-level expression measurements.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Eric,,Rappaport
| | Sample_contact_email | rappaport@email.chop.edu
| | Sample_contact_institute | Children's Hospital of Philadelphia
| | Sample_contact_address | 3615 Civic Center Blvd
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1086nnn/GSM1086183/suppl/GSM1086183_SKNAS_Monolayer_R1.CEL.gz
| | Sample_series_id | GSE44537
| | Sample_data_row_count | 54675
| | |
|
GSM1086184 | GPL570 |
|
SKNAS_Monolayer_rep_2
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Neuroblastoma cell line
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cell origin: Neuroblastoma
culture condition: Monolayer
pretreatment: None
|
|
| Sample_geo_accession | GSM1086184
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Feb 21 2013
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | SKNAS iCSC was first treated with 5AdC (2.5 uM) for five days as monolayer culture and then transferred to the serum free medium to form spheroid culture.
| | Sample_growth_protocol_ch1 | SKNAS Monolayer was grown as monolayer culture. SKNAS Sphere was grown as spheroid culture in a serum free medium. SKNAS iCSC was grown as spheroid culture in a serum free medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen RNeasy kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated amplified RNA (aRNA) was prepared from 200 ng total RNA using the standard protocol of the Ambion MessageAmp Premier Kit.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133_P_2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | Probe-level data in CEL files was normalized using GCRMA method implemented in Partek Genomics Suite to get gene-level expression measurements.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Eric,,Rappaport
| | Sample_contact_email | rappaport@email.chop.edu
| | Sample_contact_institute | Children's Hospital of Philadelphia
| | Sample_contact_address | 3615 Civic Center Blvd
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1086nnn/GSM1086184/suppl/GSM1086184_SKNAS_Monolayer_R2.CEL.gz
| | Sample_series_id | GSE44537
| | Sample_data_row_count | 54675
| | |
|
GSM1086185 | GPL570 |
|
SKNAS_Sphere_rep1
|
Neuroblastoma cell line
|
cell origin: Neuroblastoma
culture condition: Sphere
pretreatment: None
|
|
| Sample_geo_accession | GSM1086185
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Feb 21 2013
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | SKNAS iCSC was first treated with 5AdC (2.5 uM) for five days as monolayer culture and then transferred to the serum free medium to form spheroid culture.
| | Sample_growth_protocol_ch1 | SKNAS Monolayer was grown as monolayer culture. SKNAS Sphere was grown as spheroid culture in a serum free medium. SKNAS iCSC was grown as spheroid culture in a serum free medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen RNeasy kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated amplified RNA (aRNA) was prepared from 200 ng total RNA using the standard protocol of the Ambion MessageAmp Premier Kit.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133_P_2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | Probe-level data in CEL files was normalized using GCRMA method implemented in Partek Genomics Suite to get gene-level expression measurements.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Eric,,Rappaport
| | Sample_contact_email | rappaport@email.chop.edu
| | Sample_contact_institute | Children's Hospital of Philadelphia
| | Sample_contact_address | 3615 Civic Center Blvd
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1086nnn/GSM1086185/suppl/GSM1086185_SKNAS_Sphere_R1.CEL.gz
| | Sample_series_id | GSE44537
| | Sample_data_row_count | 54675
| | |
|
GSM1086186 | GPL570 |
|
SKNAS_Sphere_rep2
|
Neuroblastoma cell line
|
cell origin: Neuroblastoma
culture condition: Sphere
pretreatment: None
|
|
| Sample_geo_accession | GSM1086186
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Feb 21 2013
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | SKNAS iCSC was first treated with 5AdC (2.5 uM) for five days as monolayer culture and then transferred to the serum free medium to form spheroid culture.
| | Sample_growth_protocol_ch1 | SKNAS Monolayer was grown as monolayer culture. SKNAS Sphere was grown as spheroid culture in a serum free medium. SKNAS iCSC was grown as spheroid culture in a serum free medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen RNeasy kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated amplified RNA (aRNA) was prepared from 200 ng total RNA using the standard protocol of the Ambion MessageAmp Premier Kit.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133_P_2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | Probe-level data in CEL files was normalized using GCRMA method implemented in Partek Genomics Suite to get gene-level expression measurements.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Eric,,Rappaport
| | Sample_contact_email | rappaport@email.chop.edu
| | Sample_contact_institute | Children's Hospital of Philadelphia
| | Sample_contact_address | 3615 Civic Center Blvd
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1086nnn/GSM1086186/suppl/GSM1086186_SKNAS_Sphere_R2.CEL.gz
| | Sample_series_id | GSE44537
| | Sample_data_row_count | 54675
| | |
|
GSM1086187 | GPL570 |
|
SKNAS_iCSC_rep1
|
Neuroblastoma cell line
|
cell origin: Neuroblastoma
culture condition: Sphere
pretreatment: 5AdC treatement for five days before sphere culture
|
|
| Sample_geo_accession | GSM1086187
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Feb 21 2013
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | SKNAS iCSC was first treated with 5AdC (2.5 uM) for five days as monolayer culture and then transferred to the serum free medium to form spheroid culture.
| | Sample_growth_protocol_ch1 | SKNAS Monolayer was grown as monolayer culture. SKNAS Sphere was grown as spheroid culture in a serum free medium. SKNAS iCSC was grown as spheroid culture in a serum free medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen RNeasy kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated amplified RNA (aRNA) was prepared from 200 ng total RNA using the standard protocol of the Ambion MessageAmp Premier Kit.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133_P_2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | Probe-level data in CEL files was normalized using GCRMA method implemented in Partek Genomics Suite to get gene-level expression measurements.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Eric,,Rappaport
| | Sample_contact_email | rappaport@email.chop.edu
| | Sample_contact_institute | Children's Hospital of Philadelphia
| | Sample_contact_address | 3615 Civic Center Blvd
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1086nnn/GSM1086187/suppl/GSM1086187_SKNAS_iCSC_R1.CEL.gz
| | Sample_series_id | GSE44537
| | Sample_data_row_count | 54675
| | |
|
GSM1086188 | GPL570 |
|
SKNAS_iCSC_rep2
|
Neuroblastoma cell line
|
cell origin: Neuroblastoma
culture condition: Sphere
pretreatment: 5AdC treatement for five days before sphere culture
|
|
| Sample_geo_accession | GSM1086188
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Feb 21 2013
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | SKNAS iCSC was first treated with 5AdC (2.5 uM) for five days as monolayer culture and then transferred to the serum free medium to form spheroid culture.
| | Sample_growth_protocol_ch1 | SKNAS Monolayer was grown as monolayer culture. SKNAS Sphere was grown as spheroid culture in a serum free medium. SKNAS iCSC was grown as spheroid culture in a serum free medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen RNeasy kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated amplified RNA (aRNA) was prepared from 200 ng total RNA using the standard protocol of the Ambion MessageAmp Premier Kit.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133_P_2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | Probe-level data in CEL files was normalized using GCRMA method implemented in Partek Genomics Suite to get gene-level expression measurements.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Eric,,Rappaport
| | Sample_contact_email | rappaport@email.chop.edu
| | Sample_contact_institute | Children's Hospital of Philadelphia
| | Sample_contact_address | 3615 Civic Center Blvd
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1086nnn/GSM1086188/suppl/GSM1086188_SKNAS_iCSC_R2.CEL.gz
| | Sample_series_id | GSE44537
| | Sample_data_row_count | 54675
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