Search results for the GEO ID: GSE44560  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1086963 | GPL1355 |
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ROE2 cells-day 0 (control)
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Rat oral epithelial cell line ROE2, day 0
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cell line: ROE2
cell type: oral epithelial cell line
treatment: culture at 37˚C
time point: day 0
strain/background: Transgenic rat (Wistar strain) which has a tsSV40 large T-antigen gene [pSVtsA58ori(-)-2]
morphology: epithelial-like
life span: infinite
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The ROE2 cell line is derived from the tongue of an 18-day-old fetus.
ROE2 cells cultured for 0 days (control) at 37°C.
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| Sample_geo_accession | GSM1086963
| | Sample_status | Public on Feb 22 2013
| | Sample_submission_date | Feb 21 2013
| | Sample_last_update_date | Feb 22 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | ROE2 cells were cultured in DMEM/F12 medium supplemented with 2% FBS, 1% ITES, and 10 ng/ml EGF in a collagen type I-precoated culture dish at 33˚C. After 24 h, the culture temperature was shiftted to 37˚C, and then the cells were cultured for 0, 3, 6 and 9 days.
| | Sample_growth_protocol_ch1 | ROE2 cells were cultured in DMEM/F12 medium supplemented with 2% fetal bovine serum (FBS), 1% ITES (2.0 mg/l insulin, 2.0 mg/l transferrin, 0.122 mg/l ethanolamine and 9.14 μg/l sodium selenite), and 10 ng/ml epidermal growth factor (EGF) in a collagen type I-precoated culture dish at 33˚C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells using an RNeasy Total RNA Extraction kit (Qiagen), and treated with DNase I (Qiagen) for 15 min at room temperature to remove residual genomic DNA. RNA quality was analyzed using a Bioanalyzer 2100 (Agilent Technologies), producing RIN values between 9.5 and 10.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 500 ng of total RNA was used to synthesize cRNA with a GeneChip 3' IVT Express Kit (Affymetrix).
| | Sample_hyb_protocol | After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h. The arrays were washed, and stained with streptavidin-phycoerythrin.
| | Sample_scan_protocol | The arrays were scanned with a probe array scanner (Affymetrix).
| | Sample_data_processing | GeneChip Analysis Suite. The data were processed using RMA.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Yoshiaki,,Tabuchi
| | Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| | Sample_contact_laboratory | Division of Molecular Genetics Research
| | Sample_contact_department | Life Science Research Center
| | Sample_contact_institute | University of Toyama
| | Sample_contact_address | 2630 Sugitani
| | Sample_contact_city | Toyama
| | Sample_contact_state | Toyama
| | Sample_contact_zip/postal_code | 930-0194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1086nnn/GSM1086963/suppl/GSM1086963_ROE2-day0.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1086nnn/GSM1086963/suppl/GSM1086963_ROE2-day0.CHP.gz
| | Sample_series_id | GSE44560
| | Sample_data_row_count | 31099
| | |
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GSM1086964 | GPL1355 |
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ROE2 cells-day 3
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Rat oral epithelial cell line ROE2, day 3
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cell line: ROE2
cell type: oral epithelial cell line
treatment: culture at 37˚C
time point: day 3
strain/background: Transgenic rat (Wistar strain) which has a tsSV40 large T-antigen gene [pSVtsA58ori(-)-2]
morphology: epithelial-like
life span: infinite
|
The ROE2 cell line is derived from the tongue of an 18-day-old fetus.
ROE2 cells cultured for 3 days at 37°C.
|
| Sample_geo_accession | GSM1086964
| | Sample_status | Public on Feb 22 2013
| | Sample_submission_date | Feb 21 2013
| | Sample_last_update_date | Feb 22 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | ROE2 cells were cultured in DMEM/F12 medium supplemented with 2% FBS, 1% ITES, and 10 ng/ml EGF in a collagen type I-precoated culture dish at 33˚C. After 24 h, the culture temperature was shiftted to 37˚C, and then the cells were cultured for 0, 3, 6 and 9 days.
| | Sample_growth_protocol_ch1 | ROE2 cells were cultured in DMEM/F12 medium supplemented with 2% fetal bovine serum (FBS), 1% ITES (2.0 mg/l insulin, 2.0 mg/l transferrin, 0.122 mg/l ethanolamine and 9.14 μg/l sodium selenite), and 10 ng/ml epidermal growth factor (EGF) in a collagen type I-precoated culture dish at 33˚C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells using an RNeasy Total RNA Extraction kit (Qiagen), and treated with DNase I (Qiagen) for 15 min at room temperature to remove residual genomic DNA. RNA quality was analyzed using a Bioanalyzer 2100 (Agilent Technologies), producing RIN values between 9.5 and 10.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 500 ng of total RNA was used to synthesize cRNA with a GeneChip 3' IVT Express Kit (Affymetrix).
| | Sample_hyb_protocol | After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h. The arrays were washed, and stained with streptavidin-phycoerythrin.
| | Sample_scan_protocol | The arrays were scanned with a probe array scanner (Affymetrix).
| | Sample_data_processing | GeneChip Analysis Suite. The data were processed using RMA.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Yoshiaki,,Tabuchi
| | Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| | Sample_contact_laboratory | Division of Molecular Genetics Research
| | Sample_contact_department | Life Science Research Center
| | Sample_contact_institute | University of Toyama
| | Sample_contact_address | 2630 Sugitani
| | Sample_contact_city | Toyama
| | Sample_contact_state | Toyama
| | Sample_contact_zip/postal_code | 930-0194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1086nnn/GSM1086964/suppl/GSM1086964_ROE2-day3.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1086nnn/GSM1086964/suppl/GSM1086964_ROE2-day3.CHP.gz
| | Sample_series_id | GSE44560
| | Sample_data_row_count | 31099
| | |
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GSM1086965 | GPL1355 |
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ROE2 cells-day 6
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Rat oral epithelial cell line ROE2, day 6
|
cell line: ROE2
cell type: oral epithelial cell line
treatment: culture at 37˚C
time point: day 6
strain/background: Transgenic rat (Wistar strain) which has a tsSV40 large T-antigen gene [pSVtsA58ori(-)-2]
morphology: epithelial-like
life span: infinite
|
The ROE2 cell line is derived from the tongue of an 18-day-old fetus.
ROE2 cells cultured for 6 days at 37°C.
|
| Sample_geo_accession | GSM1086965
| | Sample_status | Public on Feb 22 2013
| | Sample_submission_date | Feb 21 2013
| | Sample_last_update_date | Feb 22 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | ROE2 cells were cultured in DMEM/F12 medium supplemented with 2% FBS, 1% ITES, and 10 ng/ml EGF in a collagen type I-precoated culture dish at 33˚C. After 24 h, the culture temperature was shiftted to 37˚C, and then the cells were cultured for 0, 3, 6 and 9 days.
| | Sample_growth_protocol_ch1 | ROE2 cells were cultured in DMEM/F12 medium supplemented with 2% fetal bovine serum (FBS), 1% ITES (2.0 mg/l insulin, 2.0 mg/l transferrin, 0.122 mg/l ethanolamine and 9.14 μg/l sodium selenite), and 10 ng/ml epidermal growth factor (EGF) in a collagen type I-precoated culture dish at 33˚C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells using an RNeasy Total RNA Extraction kit (Qiagen), and treated with DNase I (Qiagen) for 15 min at room temperature to remove residual genomic DNA. RNA quality was analyzed using a Bioanalyzer 2100 (Agilent Technologies), producing RIN values between 9.5 and 10.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 500 ng of total RNA was used to synthesize cRNA with a GeneChip 3' IVT Express Kit (Affymetrix).
| | Sample_hyb_protocol | After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h. The arrays were washed, and stained with streptavidin-phycoerythrin.
| | Sample_scan_protocol | The arrays were scanned with a probe array scanner (Affymetrix).
| | Sample_data_processing | GeneChip Analysis Suite. The data were processed using RMA.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Yoshiaki,,Tabuchi
| | Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| | Sample_contact_laboratory | Division of Molecular Genetics Research
| | Sample_contact_department | Life Science Research Center
| | Sample_contact_institute | University of Toyama
| | Sample_contact_address | 2630 Sugitani
| | Sample_contact_city | Toyama
| | Sample_contact_state | Toyama
| | Sample_contact_zip/postal_code | 930-0194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1086nnn/GSM1086965/suppl/GSM1086965_ROE2-day6.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1086nnn/GSM1086965/suppl/GSM1086965_ROE2-day6.CHP.gz
| | Sample_series_id | GSE44560
| | Sample_data_row_count | 31099
| | |
|
GSM1086966 | GPL1355 |
|
ROE2 cells-day 9
|
Rat oral epithelial cell line ROE2, day 9
|
cell line: ROE2
cell type: oral epithelial cell line
treatment: culture at 37˚C
time point: day 9
strain/background: Transgenic rat (Wistar strain) which has a tsSV40 large T-antigen gene [pSVtsA58ori(-)-2]
morphology: epithelial-like
life span: infinite
|
The ROE2 cell line is derived from the tongue of an 18-day-old fetus.
ROE2 cells cultured for 9 days at 37°C.
|
| Sample_geo_accession | GSM1086966
| | Sample_status | Public on Feb 22 2013
| | Sample_submission_date | Feb 21 2013
| | Sample_last_update_date | Feb 22 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | ROE2 cells were cultured in DMEM/F12 medium supplemented with 2% FBS, 1% ITES, and 10 ng/ml EGF in a collagen type I-precoated culture dish at 33˚C. After 24 h, the culture temperature was shiftted to 37˚C, and then the cells were cultured for 0, 3, 6 and 9 days.
| | Sample_growth_protocol_ch1 | ROE2 cells were cultured in DMEM/F12 medium supplemented with 2% fetal bovine serum (FBS), 1% ITES (2.0 mg/l insulin, 2.0 mg/l transferrin, 0.122 mg/l ethanolamine and 9.14 μg/l sodium selenite), and 10 ng/ml epidermal growth factor (EGF) in a collagen type I-precoated culture dish at 33˚C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells using an RNeasy Total RNA Extraction kit (Qiagen), and treated with DNase I (Qiagen) for 15 min at room temperature to remove residual genomic DNA. RNA quality was analyzed using a Bioanalyzer 2100 (Agilent Technologies), producing RIN values between 9.5 and 10.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 500 ng of total RNA was used to synthesize cRNA with a GeneChip 3' IVT Express Kit (Affymetrix).
| | Sample_hyb_protocol | After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h. The arrays were washed, and stained with streptavidin-phycoerythrin.
| | Sample_scan_protocol | The arrays were scanned with a probe array scanner (Affymetrix).
| | Sample_data_processing | GeneChip Analysis Suite. The data were processed using RMA.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Yoshiaki,,Tabuchi
| | Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| | Sample_contact_laboratory | Division of Molecular Genetics Research
| | Sample_contact_department | Life Science Research Center
| | Sample_contact_institute | University of Toyama
| | Sample_contact_address | 2630 Sugitani
| | Sample_contact_city | Toyama
| | Sample_contact_state | Toyama
| | Sample_contact_zip/postal_code | 930-0194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1086nnn/GSM1086966/suppl/GSM1086966_ROE2-day9.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1086nnn/GSM1086966/suppl/GSM1086966_ROE2-day9.CHP.gz
| | Sample_series_id | GSE44560
| | Sample_data_row_count | 31099
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