Search results for the GEO ID: GSE44651  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1088378 | GPL1261 |
|
WT Granulosa PMSG biological rep1
|
PMSG treated granulosa cells from WT mice
|
cell type: preovulatory granulosa cells
treatment: 48h after PMSG
genotype: WT
|
Gene expression data from WT granulosa cells treated for 48h with PMSG
|
| Sample_geo_accession | GSM1088378
| | Sample_status | Public on Jul 03 2013
| | Sample_submission_date | Feb 25 2013
| | Sample_last_update_date | Jul 03 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were collected on to LCM caps. 50 ul of Extraction buffer was added, tubes were spun and samples frozen at -80C until extraction.
| | Sample_growth_protocol_ch1 | Granulosa cells were collected from antral follicles of 3 week old WT or bERKO mice using laser micro dissection. Mice were treated with either PMSG or PMSG+hCG
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using PicoPure RNA Isolation Kit (Arcturus) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Twenty five (25) ng of total RNA was amplified as directed in the NuGEN Ovation Pico WTA System protocol, and labeled using the NuGEN Encore Biotin Module.
| | Sample_hyb_protocol | 5 μg of amplified biotin-aRNAs were fragmented and hybridized to each array for 18 hours at 45°C in a rotating hybridization. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed for antibody amplification according to the GeneChip Hybridization, Wash and Stain Kit and user manual following protocol FS450-0004.
| | Sample_scan_protocol | Arrays were scanned in an Affymetrix Scanner 3000.
| | Sample_data_processing | Data was obtained using the GeneChip® Command Console Software (AGCC; Version 1.1).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | NIEHS,,Microarray Core
| | Sample_contact_email | microarray@niehs.nih.gov, liuliw@niehs.nih.gov
| | Sample_contact_laboratory | Microarray Core
| | Sample_contact_department | DIR
| | Sample_contact_institute | NIEHS
| | Sample_contact_address | 111 T.W. Alexander Drive
| | Sample_contact_city | RTP
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1088nnn/GSM1088378/suppl/GSM1088378_9369_532_WT_GRan_Rep_1_Mouse430_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1088nnn/GSM1088378/suppl/GSM1088378_9369_532_WT_GRan_Rep_1_Mouse430_2_.mas5.CHP.gz
| | Sample_series_id | GSE44651
| | Sample_data_row_count | 45101
| | |
|
GSM1088379 | GPL1261 |
|
WT Granulosa PMSG biological rep2
|
PMSG treated granulosa cells from WT mice
|
cell type: preovulatory granulosa cells
treatment: 48h after PMSG
genotype: WT
|
Gene expression data from WT granulosa cells treated for 48h with PMSG
|
| Sample_geo_accession | GSM1088379
| | Sample_status | Public on Jul 03 2013
| | Sample_submission_date | Feb 25 2013
| | Sample_last_update_date | Jul 03 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were collected on to LCM caps. 50 ul of Extraction buffer was added, tubes were spun and samples frozen at -80C until extraction.
| | Sample_growth_protocol_ch1 | Granulosa cells were collected from antral follicles of 3 week old WT or bERKO mice using laser micro dissection. Mice were treated with either PMSG or PMSG+hCG
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using PicoPure RNA Isolation Kit (Arcturus) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Twenty five (25) ng of total RNA was amplified as directed in the NuGEN Ovation Pico WTA System protocol, and labeled using the NuGEN Encore Biotin Module.
| | Sample_hyb_protocol | 5 μg of amplified biotin-aRNAs were fragmented and hybridized to each array for 18 hours at 45°C in a rotating hybridization. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed for antibody amplification according to the GeneChip Hybridization, Wash and Stain Kit and user manual following protocol FS450-0004.
| | Sample_scan_protocol | Arrays were scanned in an Affymetrix Scanner 3000.
| | Sample_data_processing | Data was obtained using the GeneChip® Command Console Software (AGCC; Version 1.1).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | NIEHS,,Microarray Core
| | Sample_contact_email | microarray@niehs.nih.gov, liuliw@niehs.nih.gov
| | Sample_contact_laboratory | Microarray Core
| | Sample_contact_department | DIR
| | Sample_contact_institute | NIEHS
| | Sample_contact_address | 111 T.W. Alexander Drive
| | Sample_contact_city | RTP
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1088nnn/GSM1088379/suppl/GSM1088379_9370_532_WT_Gran_Rep_2_Mouse430_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1088nnn/GSM1088379/suppl/GSM1088379_9370_532_WT_Gran_Rep_2_Mouse430_2_.mas5.CHP.gz
| | Sample_series_id | GSE44651
| | Sample_data_row_count | 45101
| | |
|
GSM1088380 | GPL1261 |
|
WT Granulosa PMSG biological rep3
|
PMSG treated granulosa cells from WT mice
|
cell type: preovulatory granulosa cells
treatment: 48h after PMSG
genotype: WT
|
Gene expression data from WT granulosa cells treated for 48h with PMSG
|
| Sample_geo_accession | GSM1088380
| | Sample_status | Public on Jul 03 2013
| | Sample_submission_date | Feb 25 2013
| | Sample_last_update_date | Jul 03 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were collected on to LCM caps. 50 ul of Extraction buffer was added, tubes were spun and samples frozen at -80C until extraction.
| | Sample_growth_protocol_ch1 | Granulosa cells were collected from antral follicles of 3 week old WT or bERKO mice using laser micro dissection. Mice were treated with either PMSG or PMSG+hCG
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using PicoPure RNA Isolation Kit (Arcturus) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Twenty five (25) ng of total RNA was amplified as directed in the NuGEN Ovation Pico WTA System protocol, and labeled using the NuGEN Encore Biotin Module.
| | Sample_hyb_protocol | 5 μg of amplified biotin-aRNAs were fragmented and hybridized to each array for 18 hours at 45°C in a rotating hybridization. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed for antibody amplification according to the GeneChip Hybridization, Wash and Stain Kit and user manual following protocol FS450-0004.
| | Sample_scan_protocol | Arrays were scanned in an Affymetrix Scanner 3000.
| | Sample_data_processing | Data was obtained using the GeneChip® Command Console Software (AGCC; Version 1.1).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | NIEHS,,Microarray Core
| | Sample_contact_email | microarray@niehs.nih.gov, liuliw@niehs.nih.gov
| | Sample_contact_laboratory | Microarray Core
| | Sample_contact_department | DIR
| | Sample_contact_institute | NIEHS
| | Sample_contact_address | 111 T.W. Alexander Drive
| | Sample_contact_city | RTP
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1088nnn/GSM1088380/suppl/GSM1088380_9371_532_WT_Gran_Rep_3_Mouse430_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1088nnn/GSM1088380/suppl/GSM1088380_9371_532_WT_Gran_Rep_3_Mouse430_2_.mas5.CHP.gz
| | Sample_series_id | GSE44651
| | Sample_data_row_count | 45101
| | |
|
GSM1088381 | GPL1261 |
|
bERKO Granulosa PMSG biological rep1
|
PMSG treated granulosa cells from bERKO mice
|
cell type: preovulatory granulosa cells
treatment: 48h after PMSG
genotype: bERKO
|
Gene expression data from bERKO granulosa cells treated for 48h with PMSG
|
| Sample_geo_accession | GSM1088381
| | Sample_status | Public on Jul 03 2013
| | Sample_submission_date | Feb 25 2013
| | Sample_last_update_date | Jul 03 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were collected on to LCM caps. 50 ul of Extraction buffer was added, tubes were spun and samples frozen at -80C until extraction.
| | Sample_growth_protocol_ch1 | Granulosa cells were collected from antral follicles of 3 week old WT or bERKO mice using laser micro dissection. Mice were treated with either PMSG or PMSG+hCG
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using PicoPure RNA Isolation Kit (Arcturus) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Twenty five (25) ng of total RNA was amplified as directed in the NuGEN Ovation Pico WTA System protocol, and labeled using the NuGEN Encore Biotin Module.
| | Sample_hyb_protocol | 5 μg of amplified biotin-aRNAs were fragmented and hybridized to each array for 18 hours at 45°C in a rotating hybridization. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed for antibody amplification according to the GeneChip Hybridization, Wash and Stain Kit and user manual following protocol FS450-0004.
| | Sample_scan_protocol | Arrays were scanned in an Affymetrix Scanner 3000.
| | Sample_data_processing | Data was obtained using the GeneChip® Command Console Software (AGCC; Version 1.1).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | NIEHS,,Microarray Core
| | Sample_contact_email | microarray@niehs.nih.gov, liuliw@niehs.nih.gov
| | Sample_contact_laboratory | Microarray Core
| | Sample_contact_department | DIR
| | Sample_contact_institute | NIEHS
| | Sample_contact_address | 111 T.W. Alexander Drive
| | Sample_contact_city | RTP
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1088nnn/GSM1088381/suppl/GSM1088381_9372_532_KO_Gran_Rep_1_Mouse430_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1088nnn/GSM1088381/suppl/GSM1088381_9372_532_KO_Gran_Rep_1_Mouse430_2_.mas5.CHP.gz
| | Sample_series_id | GSE44651
| | Sample_data_row_count | 45101
| | |
|
GSM1088382 | GPL1261 |
|
bERKO Granulosa PMSG biological rep2
|
PMSG treated granulosa cells from bERKO mice
|
cell type: preovulatory granulosa cells
treatment: 48h after PMSG
genotype: bERKO
|
Gene expression data from bERKO granulosa cells treated for 48h with PMSG
|
| Sample_geo_accession | GSM1088382
| | Sample_status | Public on Jul 03 2013
| | Sample_submission_date | Feb 25 2013
| | Sample_last_update_date | Jul 03 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were collected on to LCM caps. 50 ul of Extraction buffer was added, tubes were spun and samples frozen at -80C until extraction.
| | Sample_growth_protocol_ch1 | Granulosa cells were collected from antral follicles of 3 week old WT or bERKO mice using laser micro dissection. Mice were treated with either PMSG or PMSG+hCG
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using PicoPure RNA Isolation Kit (Arcturus) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Twenty five (25) ng of total RNA was amplified as directed in the NuGEN Ovation Pico WTA System protocol, and labeled using the NuGEN Encore Biotin Module.
| | Sample_hyb_protocol | 5 μg of amplified biotin-aRNAs were fragmented and hybridized to each array for 18 hours at 45°C in a rotating hybridization. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed for antibody amplification according to the GeneChip Hybridization, Wash and Stain Kit and user manual following protocol FS450-0004.
| | Sample_scan_protocol | Arrays were scanned in an Affymetrix Scanner 3000.
| | Sample_data_processing | Data was obtained using the GeneChip® Command Console Software (AGCC; Version 1.1).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | NIEHS,,Microarray Core
| | Sample_contact_email | microarray@niehs.nih.gov, liuliw@niehs.nih.gov
| | Sample_contact_laboratory | Microarray Core
| | Sample_contact_department | DIR
| | Sample_contact_institute | NIEHS
| | Sample_contact_address | 111 T.W. Alexander Drive
| | Sample_contact_city | RTP
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1088nnn/GSM1088382/suppl/GSM1088382_9373_532_KO_Gran_Rep_2_Mouse430_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1088nnn/GSM1088382/suppl/GSM1088382_9373_532_KO_Gran_Rep_2_Mouse430_2_.mas5.CHP.gz
| | Sample_series_id | GSE44651
| | Sample_data_row_count | 45101
| | |
|
GSM1088383 | GPL1261 |
|
bERKO Granulosa PMSG biological rep3
|
PMSG treated granulosa cells from bERKO mice
|
cell type: preovulatory granulosa cells
treatment: 48h after PMSG
genotype: bERKO
|
Gene expression data from bERKO granulosa cells treated for 48h with PMSG
|
| Sample_geo_accession | GSM1088383
| | Sample_status | Public on Jul 03 2013
| | Sample_submission_date | Feb 25 2013
| | Sample_last_update_date | Jul 03 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were collected on to LCM caps. 50 ul of Extraction buffer was added, tubes were spun and samples frozen at -80C until extraction.
| | Sample_growth_protocol_ch1 | Granulosa cells were collected from antral follicles of 3 week old WT or bERKO mice using laser micro dissection. Mice were treated with either PMSG or PMSG+hCG
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using PicoPure RNA Isolation Kit (Arcturus) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Twenty five (25) ng of total RNA was amplified as directed in the NuGEN Ovation Pico WTA System protocol, and labeled using the NuGEN Encore Biotin Module.
| | Sample_hyb_protocol | 5 μg of amplified biotin-aRNAs were fragmented and hybridized to each array for 18 hours at 45°C in a rotating hybridization. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed for antibody amplification according to the GeneChip Hybridization, Wash and Stain Kit and user manual following protocol FS450-0004.
| | Sample_scan_protocol | Arrays were scanned in an Affymetrix Scanner 3000.
| | Sample_data_processing | Data was obtained using the GeneChip® Command Console Software (AGCC; Version 1.1).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | NIEHS,,Microarray Core
| | Sample_contact_email | microarray@niehs.nih.gov, liuliw@niehs.nih.gov
| | Sample_contact_laboratory | Microarray Core
| | Sample_contact_department | DIR
| | Sample_contact_institute | NIEHS
| | Sample_contact_address | 111 T.W. Alexander Drive
| | Sample_contact_city | RTP
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1088nnn/GSM1088383/suppl/GSM1088383_9374_532_KO_Gran_Rep_3_Mouse430_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1088nnn/GSM1088383/suppl/GSM1088383_9374_532_KO_Gran_Rep_3_Mouse430_2_.mas5.CHP.gz
| | Sample_series_id | GSE44651
| | Sample_data_row_count | 45101
| | |
|
GSM1088384 | GPL1261 |
|
WT Granulosa hCG biological rep1
|
PMSG/hCG treated granulosa cells from WT mice
|
cell type: preovulatory granulosa cells
treatment: 48h after PMSG + 4 h after hCG
genotype: WT
|
Gene expression data from WT granulosa cells treated for 48h with PMSG followed by 4h with hCG
|
| Sample_geo_accession | GSM1088384
| | Sample_status | Public on Jul 03 2013
| | Sample_submission_date | Feb 25 2013
| | Sample_last_update_date | Jul 03 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were collected on to LCM caps. 50 ul of Extraction buffer was added, tubes were spun and samples frozen at -80C until extraction.
| | Sample_growth_protocol_ch1 | Granulosa cells were collected from antral follicles of 3 week old WT or bERKO mice using laser micro dissection. Mice were treated with either PMSG or PMSG+hCG
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using PicoPure RNA Isolation Kit (Arcturus) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Twenty five (25) ng of total RNA was amplified as directed in the NuGEN Ovation Pico WTA System protocol, and labeled using the NuGEN Encore Biotin Module.
| | Sample_hyb_protocol | 5 μg of amplified biotin-aRNAs were fragmented and hybridized to each array for 18 hours at 45°C in a rotating hybridization. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed for antibody amplification according to the GeneChip Hybridization, Wash and Stain Kit and user manual following protocol FS450-0004.
| | Sample_scan_protocol | Arrays were scanned in an Affymetrix Scanner 3000.
| | Sample_data_processing | Data was obtained using the GeneChip® Command Console Software (AGCC; Version 1.1).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | NIEHS,,Microarray Core
| | Sample_contact_email | microarray@niehs.nih.gov, liuliw@niehs.nih.gov
| | Sample_contact_laboratory | Microarray Core
| | Sample_contact_department | DIR
| | Sample_contact_institute | NIEHS
| | Sample_contact_address | 111 T.W. Alexander Drive
| | Sample_contact_city | RTP
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1088nnn/GSM1088384/suppl/GSM1088384_10650_532_GRAN_KO_Rep_1_Mouse430_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1088nnn/GSM1088384/suppl/GSM1088384_10650_532_GRAN_KO_Rep_1_Mouse430_2_.mas5.CHP.gz
| | Sample_series_id | GSE44651
| | Sample_data_row_count | 45101
| | |
|
GSM1088385 | GPL1261 |
|
WT Granulosa hCG biological rep2
|
PMSG/hCG treated granulosa cells from WT mice
|
cell type: preovulatory granulosa cells
treatment: 48h after PMSG + 4 h after hCG
genotype: WT
|
Gene expression data from WT granulosa cells treated for 48h with PMSG followed by 4h with hCG
|
| Sample_geo_accession | GSM1088385
| | Sample_status | Public on Jul 03 2013
| | Sample_submission_date | Feb 25 2013
| | Sample_last_update_date | Jul 03 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were collected on to LCM caps. 50 ul of Extraction buffer was added, tubes were spun and samples frozen at -80C until extraction.
| | Sample_growth_protocol_ch1 | Granulosa cells were collected from antral follicles of 3 week old WT or bERKO mice using laser micro dissection. Mice were treated with either PMSG or PMSG+hCG
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using PicoPure RNA Isolation Kit (Arcturus) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Twenty five (25) ng of total RNA was amplified as directed in the NuGEN Ovation Pico WTA System protocol, and labeled using the NuGEN Encore Biotin Module.
| | Sample_hyb_protocol | 5 μg of amplified biotin-aRNAs were fragmented and hybridized to each array for 18 hours at 45°C in a rotating hybridization. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed for antibody amplification according to the GeneChip Hybridization, Wash and Stain Kit and user manual following protocol FS450-0004.
| | Sample_scan_protocol | Arrays were scanned in an Affymetrix Scanner 3000.
| | Sample_data_processing | Data was obtained using the GeneChip® Command Console Software (AGCC; Version 1.1).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | NIEHS,,Microarray Core
| | Sample_contact_email | microarray@niehs.nih.gov, liuliw@niehs.nih.gov
| | Sample_contact_laboratory | Microarray Core
| | Sample_contact_department | DIR
| | Sample_contact_institute | NIEHS
| | Sample_contact_address | 111 T.W. Alexander Drive
| | Sample_contact_city | RTP
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1088nnn/GSM1088385/suppl/GSM1088385_10651_532_GRAN_KO_Rep_2_Mouse430_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1088nnn/GSM1088385/suppl/GSM1088385_10651_532_GRAN_KO_Rep_2_Mouse430_2_.mas5.CHP.gz
| | Sample_series_id | GSE44651
| | Sample_data_row_count | 45101
| | |
|
GSM1088386 | GPL1261 |
|
WT Granulosa hCG biological rep3
|
PMSG/hCG treated granulosa cells from WT mice
|
cell type: preovulatory granulosa cells
treatment: 48h after PMSG + 4 h after hCG
genotype: WT
|
Gene expression data from WT granulosa cells treated for 48h with PMSG followed by 4h with hCG
|
| Sample_geo_accession | GSM1088386
| | Sample_status | Public on Jul 03 2013
| | Sample_submission_date | Feb 25 2013
| | Sample_last_update_date | Jul 03 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were collected on to LCM caps. 50 ul of Extraction buffer was added, tubes were spun and samples frozen at -80C until extraction.
| | Sample_growth_protocol_ch1 | Granulosa cells were collected from antral follicles of 3 week old WT or bERKO mice using laser micro dissection. Mice were treated with either PMSG or PMSG+hCG
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using PicoPure RNA Isolation Kit (Arcturus) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Twenty five (25) ng of total RNA was amplified as directed in the NuGEN Ovation Pico WTA System protocol, and labeled using the NuGEN Encore Biotin Module.
| | Sample_hyb_protocol | 5 μg of amplified biotin-aRNAs were fragmented and hybridized to each array for 18 hours at 45°C in a rotating hybridization. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed for antibody amplification according to the GeneChip Hybridization, Wash and Stain Kit and user manual following protocol FS450-0004.
| | Sample_scan_protocol | Arrays were scanned in an Affymetrix Scanner 3000.
| | Sample_data_processing | Data was obtained using the GeneChip® Command Console Software (AGCC; Version 1.1).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | NIEHS,,Microarray Core
| | Sample_contact_email | microarray@niehs.nih.gov, liuliw@niehs.nih.gov
| | Sample_contact_laboratory | Microarray Core
| | Sample_contact_department | DIR
| | Sample_contact_institute | NIEHS
| | Sample_contact_address | 111 T.W. Alexander Drive
| | Sample_contact_city | RTP
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1088nnn/GSM1088386/suppl/GSM1088386_10652_532_GRAN_KO_Rep_3_Mouse430_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1088nnn/GSM1088386/suppl/GSM1088386_10652_532_GRAN_KO_Rep_3_Mouse430_2_.mas5.CHP.gz
| | Sample_series_id | GSE44651
| | Sample_data_row_count | 45101
| | |
|
GSM1088387 | GPL1261 |
|
bERKO Granulosa hCG biological rep1
|
PMSG/hCG treated granulosa cells from bERKO mice
|
cell type: preovulatory granulosa cells
treatment: 48h after PMSG + 4 h after hCG
genotype: bERKO
|
Gene expression data from bERKO granulosa cells treated for 48h with PMSG followed by 4h with hCG
|
| Sample_geo_accession | GSM1088387
| | Sample_status | Public on Jul 03 2013
| | Sample_submission_date | Feb 25 2013
| | Sample_last_update_date | Jul 03 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were collected on to LCM caps. 50 ul of Extraction buffer was added, tubes were spun and samples frozen at -80C until extraction.
| | Sample_growth_protocol_ch1 | Granulosa cells were collected from antral follicles of 3 week old WT or bERKO mice using laser micro dissection. Mice were treated with either PMSG or PMSG+hCG
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using PicoPure RNA Isolation Kit (Arcturus) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Twenty five (25) ng of total RNA was amplified as directed in the NuGEN Ovation Pico WTA System protocol, and labeled using the NuGEN Encore Biotin Module.
| | Sample_hyb_protocol | 5 μg of amplified biotin-aRNAs were fragmented and hybridized to each array for 18 hours at 45°C in a rotating hybridization. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed for antibody amplification according to the GeneChip Hybridization, Wash and Stain Kit and user manual following protocol FS450-0004.
| | Sample_scan_protocol | Arrays were scanned in an Affymetrix Scanner 3000.
| | Sample_data_processing | Data was obtained using the GeneChip® Command Console Software (AGCC; Version 1.1).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | NIEHS,,Microarray Core
| | Sample_contact_email | microarray@niehs.nih.gov, liuliw@niehs.nih.gov
| | Sample_contact_laboratory | Microarray Core
| | Sample_contact_department | DIR
| | Sample_contact_institute | NIEHS
| | Sample_contact_address | 111 T.W. Alexander Drive
| | Sample_contact_city | RTP
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1088nnn/GSM1088387/suppl/GSM1088387_10653_532_GRAN_WT_Rep_1_Mouse430_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1088nnn/GSM1088387/suppl/GSM1088387_10653_532_GRAN_WT_Rep_1_Mouse430_2_.mas5.CHP.gz
| | Sample_series_id | GSE44651
| | Sample_data_row_count | 45101
| | |
|
GSM1088388 | GPL1261 |
|
bERKO Granulosa hCG biological rep2
|
PMSG/hCG treated granulosa cells from bERKO mice
|
cell type: preovulatory granulosa cells
treatment: 48h after PMSG + 4 h after hCG
genotype: bERKO
|
Gene expression data from bERKO granulosa cells treated for 48h with PMSG followed by 4h with hCG
|
| Sample_geo_accession | GSM1088388
| | Sample_status | Public on Jul 03 2013
| | Sample_submission_date | Feb 25 2013
| | Sample_last_update_date | Jul 03 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were collected on to LCM caps. 50 ul of Extraction buffer was added, tubes were spun and samples frozen at -80C until extraction.
| | Sample_growth_protocol_ch1 | Granulosa cells were collected from antral follicles of 3 week old WT or bERKO mice using laser micro dissection. Mice were treated with either PMSG or PMSG+hCG
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using PicoPure RNA Isolation Kit (Arcturus) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Twenty five (25) ng of total RNA was amplified as directed in the NuGEN Ovation Pico WTA System protocol, and labeled using the NuGEN Encore Biotin Module.
| | Sample_hyb_protocol | 5 μg of amplified biotin-aRNAs were fragmented and hybridized to each array for 18 hours at 45°C in a rotating hybridization. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed for antibody amplification according to the GeneChip Hybridization, Wash and Stain Kit and user manual following protocol FS450-0004.
| | Sample_scan_protocol | Arrays were scanned in an Affymetrix Scanner 3000.
| | Sample_data_processing | Data was obtained using the GeneChip® Command Console Software (AGCC; Version 1.1).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | NIEHS,,Microarray Core
| | Sample_contact_email | microarray@niehs.nih.gov, liuliw@niehs.nih.gov
| | Sample_contact_laboratory | Microarray Core
| | Sample_contact_department | DIR
| | Sample_contact_institute | NIEHS
| | Sample_contact_address | 111 T.W. Alexander Drive
| | Sample_contact_city | RTP
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1088nnn/GSM1088388/suppl/GSM1088388_10654_532_GRAN_WT_Rep_2_Mouse430_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1088nnn/GSM1088388/suppl/GSM1088388_10654_532_GRAN_WT_Rep_2_Mouse430_2_.mas5.CHP.gz
| | Sample_series_id | GSE44651
| | Sample_data_row_count | 45101
| | |
|
GSM1088389 | GPL1261 |
|
bERKO Granulosa hCG biological rep3
|
PMSG/hCG treated granulosa cells from bERKO mice
|
cell type: preovulatory granulosa cells
treatment: 48h after PMSG + 4 h after hCG
genotype: bERKO
|
Gene expression data from bERKO granulosa cells treated for 48h with PMSG followed by 4h with hCG
|
| Sample_geo_accession | GSM1088389
| | Sample_status | Public on Jul 03 2013
| | Sample_submission_date | Feb 25 2013
| | Sample_last_update_date | Jul 03 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were collected on to LCM caps. 50 ul of Extraction buffer was added, tubes were spun and samples frozen at -80C until extraction.
| | Sample_growth_protocol_ch1 | Granulosa cells were collected from antral follicles of 3 week old WT or bERKO mice using laser micro dissection. Mice were treated with either PMSG or PMSG+hCG
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using PicoPure RNA Isolation Kit (Arcturus) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Twenty five (25) ng of total RNA was amplified as directed in the NuGEN Ovation Pico WTA System protocol, and labeled using the NuGEN Encore Biotin Module.
| | Sample_hyb_protocol | 5 μg of amplified biotin-aRNAs were fragmented and hybridized to each array for 18 hours at 45°C in a rotating hybridization. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed for antibody amplification according to the GeneChip Hybridization, Wash and Stain Kit and user manual following protocol FS450-0004.
| | Sample_scan_protocol | Arrays were scanned in an Affymetrix Scanner 3000.
| | Sample_data_processing | Data was obtained using the GeneChip® Command Console Software (AGCC; Version 1.1).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | NIEHS,,Microarray Core
| | Sample_contact_email | microarray@niehs.nih.gov, liuliw@niehs.nih.gov
| | Sample_contact_laboratory | Microarray Core
| | Sample_contact_department | DIR
| | Sample_contact_institute | NIEHS
| | Sample_contact_address | 111 T.W. Alexander Drive
| | Sample_contact_city | RTP
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1088nnn/GSM1088389/suppl/GSM1088389_10655_532_GRAN_WT_Rep_3_Mouse430_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1088nnn/GSM1088389/suppl/GSM1088389_10655_532_GRAN_WT_Rep_3_Mouse430_2_.mas5.CHP.gz
| | Sample_series_id | GSE44651
| | Sample_data_row_count | 45101
| | |
|
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Make groups for comparisons |
(2 groups will be compared at a time) |
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| Select GSMs and click on "Add groups" |
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