Search results for the GEO ID: GSE44734  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1089795 | GPL1261 |
|
BDNF Wild Type, biological rep1
|
Wild-type geniculate ganglion at E14.5
|
tissue: geniculate ganglion
genotype: BDNF wild type
age: Embryonic day 14.5
background mouse strain: C57BL/6J
|
Gene expression data from BDNF wild-type geniculate ganglia at E14.5
|
| Sample_geo_accession | GSM1089795
| | Sample_status | Public on Feb 28 2013
| | Sample_submission_date | Feb 27 2013
| | Sample_last_update_date | Feb 28 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from geniculate ganglion using RNeasy micro kit. DNase I treatment was applied to eliminate traces of DNA during the procedure.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified, fragmented, and biotinylated using WT-Ovation Pico RNA Amplification System.
| | Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Mouse Genome 430 2.0 arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | dChip software was used to perform normalization and model-based expression value calculations. Different microarray samples were normalized using the baseline array that has a median probe signal, and the PM-MM difference model was applied for expression value calculation. Genes were considered to be differentially regulated if they met all 3 of the following criteria: 1) the fold change between knockout and wild-type mice exceeds 1.2; 2) the threshold for the absolute difference between knockout vs. wild type means is larger than 50; 3) the p-value of unpaired t-test is less than 0.05.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tao,,Huang
| | Sample_contact_email | t0huan02@louisville.edu
| | Sample_contact_phone | 5028523059
| | Sample_contact_fax | 5028526228
| | Sample_contact_department | Anatomical Sciences and Neurobiology
| | Sample_contact_institute | University of Louisville
| | Sample_contact_address | 500 S Preston St, Rm1004
| | Sample_contact_city | Louisville
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1089nnn/GSM1089795/suppl/GSM1089795_BDNF_WT_A.CEL.gz
| | Sample_series_id | GSE44734
| | Sample_data_row_count | 45101
| | |
|
GSM1089796 | GPL1261 |
|
BDNF Wild Type, biological rep2
|
Wild-type geniculate ganglion at E14.5
|
tissue: geniculate ganglion
genotype: BDNF wild type
age: Embryonic day 14.5
background mouse strain: C57BL/6J
|
Gene expression data from BDNF wild-type geniculate ganglia at E14.5
|
| Sample_geo_accession | GSM1089796
| | Sample_status | Public on Feb 28 2013
| | Sample_submission_date | Feb 27 2013
| | Sample_last_update_date | Feb 28 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from geniculate ganglion using RNeasy micro kit. DNase I treatment was applied to eliminate traces of DNA during the procedure.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified, fragmented, and biotinylated using WT-Ovation Pico RNA Amplification System.
| | Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Mouse Genome 430 2.0 arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | dChip software was used to perform normalization and model-based expression value calculations. Different microarray samples were normalized using the baseline array that has a median probe signal, and the PM-MM difference model was applied for expression value calculation. Genes were considered to be differentially regulated if they met all 3 of the following criteria: 1) the fold change between knockout and wild-type mice exceeds 1.2; 2) the threshold for the absolute difference between knockout vs. wild type means is larger than 50; 3) the p-value of unpaired t-test is less than 0.05.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tao,,Huang
| | Sample_contact_email | t0huan02@louisville.edu
| | Sample_contact_phone | 5028523059
| | Sample_contact_fax | 5028526228
| | Sample_contact_department | Anatomical Sciences and Neurobiology
| | Sample_contact_institute | University of Louisville
| | Sample_contact_address | 500 S Preston St, Rm1004
| | Sample_contact_city | Louisville
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1089nnn/GSM1089796/suppl/GSM1089796_BDNF_WT_B.CEL.gz
| | Sample_series_id | GSE44734
| | Sample_data_row_count | 45101
| | |
|
GSM1089797 | GPL1261 |
|
BDNF Wild Type, biological rep3
|
Wild-type geniculate ganglion at E14.5
|
tissue: geniculate ganglion
genotype: BDNF wild type
age: Embryonic day 14.5
background mouse strain: C57BL/6J
|
Gene expression data from BDNF wild-type geniculate ganglia at E14.5
|
| Sample_geo_accession | GSM1089797
| | Sample_status | Public on Feb 28 2013
| | Sample_submission_date | Feb 27 2013
| | Sample_last_update_date | Feb 28 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from geniculate ganglion using RNeasy micro kit. DNase I treatment was applied to eliminate traces of DNA during the procedure.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified, fragmented, and biotinylated using WT-Ovation Pico RNA Amplification System.
| | Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Mouse Genome 430 2.0 arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | dChip software was used to perform normalization and model-based expression value calculations. Different microarray samples were normalized using the baseline array that has a median probe signal, and the PM-MM difference model was applied for expression value calculation. Genes were considered to be differentially regulated if they met all 3 of the following criteria: 1) the fold change between knockout and wild-type mice exceeds 1.2; 2) the threshold for the absolute difference between knockout vs. wild type means is larger than 50; 3) the p-value of unpaired t-test is less than 0.05.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tao,,Huang
| | Sample_contact_email | t0huan02@louisville.edu
| | Sample_contact_phone | 5028523059
| | Sample_contact_fax | 5028526228
| | Sample_contact_department | Anatomical Sciences and Neurobiology
| | Sample_contact_institute | University of Louisville
| | Sample_contact_address | 500 S Preston St, Rm1004
| | Sample_contact_city | Louisville
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1089nnn/GSM1089797/suppl/GSM1089797_BDNF_WT_C.CEL.gz
| | Sample_series_id | GSE44734
| | Sample_data_row_count | 45101
| | |
|
GSM1089798 | GPL1261 |
|
BDNF Knockout, biological rep1
|
BDNF knockout geniculate ganglion at E14.5
|
tissue: geniculate ganglion
genotype: BDNF knockout
age: Embryonic day 14.5
background mouse strain: C57BL/6J
|
Gene expression data from BDNF mutant geniculate ganglia at E14.5
|
| Sample_geo_accession | GSM1089798
| | Sample_status | Public on Feb 28 2013
| | Sample_submission_date | Feb 27 2013
| | Sample_last_update_date | Feb 28 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from geniculate ganglion using RNeasy micro kit. DNase I treatment was applied to eliminate traces of DNA during the procedure.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified, fragmented, and biotinylated using WT-Ovation Pico RNA Amplification System.
| | Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Mouse Genome 430 2.0 arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | dChip software was used to perform normalization and model-based expression value calculations. Different microarray samples were normalized using the baseline array that has a median probe signal, and the PM-MM difference model was applied for expression value calculation. Genes were considered to be differentially regulated if they met all 3 of the following criteria: 1) the fold change between knockout and wild-type mice exceeds 1.2; 2) the threshold for the absolute difference between knockout vs. wild type means is larger than 50; 3) the p-value of unpaired t-test is less than 0.05.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tao,,Huang
| | Sample_contact_email | t0huan02@louisville.edu
| | Sample_contact_phone | 5028523059
| | Sample_contact_fax | 5028526228
| | Sample_contact_department | Anatomical Sciences and Neurobiology
| | Sample_contact_institute | University of Louisville
| | Sample_contact_address | 500 S Preston St, Rm1004
| | Sample_contact_city | Louisville
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1089nnn/GSM1089798/suppl/GSM1089798_BDNF_KO_A.CEL.gz
| | Sample_series_id | GSE44734
| | Sample_data_row_count | 45101
| | |
|
GSM1089799 | GPL1261 |
|
BDNF Knockout, biological rep2
|
BDNF knockout geniculate ganglion at E14.5
|
tissue: geniculate ganglion
genotype: BDNF knockout
age: Embryonic day 14.5
background mouse strain: C57BL/6J
|
Gene expression data from BDNF mutant geniculate ganglia at E14.5
|
| Sample_geo_accession | GSM1089799
| | Sample_status | Public on Feb 28 2013
| | Sample_submission_date | Feb 27 2013
| | Sample_last_update_date | Feb 28 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from geniculate ganglion using RNeasy micro kit. DNase I treatment was applied to eliminate traces of DNA during the procedure.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified, fragmented, and biotinylated using WT-Ovation Pico RNA Amplification System.
| | Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Mouse Genome 430 2.0 arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | dChip software was used to perform normalization and model-based expression value calculations. Different microarray samples were normalized using the baseline array that has a median probe signal, and the PM-MM difference model was applied for expression value calculation. Genes were considered to be differentially regulated if they met all 3 of the following criteria: 1) the fold change between knockout and wild-type mice exceeds 1.2; 2) the threshold for the absolute difference between knockout vs. wild type means is larger than 50; 3) the p-value of unpaired t-test is less than 0.05.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tao,,Huang
| | Sample_contact_email | t0huan02@louisville.edu
| | Sample_contact_phone | 5028523059
| | Sample_contact_fax | 5028526228
| | Sample_contact_department | Anatomical Sciences and Neurobiology
| | Sample_contact_institute | University of Louisville
| | Sample_contact_address | 500 S Preston St, Rm1004
| | Sample_contact_city | Louisville
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1089nnn/GSM1089799/suppl/GSM1089799_BDNF_KO_B.CEL.gz
| | Sample_series_id | GSE44734
| | Sample_data_row_count | 45101
| | |
|
GSM1089800 | GPL1261 |
|
BDNF Knockout, biological rep3
|
BDNF knockout geniculate ganglion at E14.5
|
tissue: geniculate ganglion
genotype: BDNF knockout
age: Embryonic day 14.5
background mouse strain: C57BL/6J
|
Gene expression data from BDNF mutant geniculate ganglia at E14.5
|
| Sample_geo_accession | GSM1089800
| | Sample_status | Public on Feb 28 2013
| | Sample_submission_date | Feb 27 2013
| | Sample_last_update_date | Feb 28 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from geniculate ganglion using RNeasy micro kit. DNase I treatment was applied to eliminate traces of DNA during the procedure.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified, fragmented, and biotinylated using WT-Ovation Pico RNA Amplification System.
| | Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Mouse Genome 430 2.0 arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | dChip software was used to perform normalization and model-based expression value calculations. Different microarray samples were normalized using the baseline array that has a median probe signal, and the PM-MM difference model was applied for expression value calculation. Genes were considered to be differentially regulated if they met all 3 of the following criteria: 1) the fold change between knockout and wild-type mice exceeds 1.2; 2) the threshold for the absolute difference between knockout vs. wild type means is larger than 50; 3) the p-value of unpaired t-test is less than 0.05.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tao,,Huang
| | Sample_contact_email | t0huan02@louisville.edu
| | Sample_contact_phone | 5028523059
| | Sample_contact_fax | 5028526228
| | Sample_contact_department | Anatomical Sciences and Neurobiology
| | Sample_contact_institute | University of Louisville
| | Sample_contact_address | 500 S Preston St, Rm1004
| | Sample_contact_city | Louisville
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1089nnn/GSM1089800/suppl/GSM1089800_BDNF_KO_C.CEL.gz
| | Sample_series_id | GSE44734
| | Sample_data_row_count | 45101
| | |
|
GSM1089801 | GPL1261 |
|
NT4 Wild Type, biological rep1
|
Wild-type geniculate ganglion at E14.5
|
tissue: geniculate ganglion
genotype: NT4 wild type
age: Embryonic day 14.5
background mouse strain: 129
|
Gene expression data from NT4 wild-type geniculate ganglia at E14.5
|
| Sample_geo_accession | GSM1089801
| | Sample_status | Public on Feb 28 2013
| | Sample_submission_date | Feb 27 2013
| | Sample_last_update_date | Feb 28 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from geniculate ganglion using RNeasy micro kit. DNase I treatment was applied to eliminate traces of DNA during the procedure.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified, fragmented, and biotinylated using WT-Ovation Pico RNA Amplification System.
| | Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Mouse Genome 430 2.0 arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | dChip software was used to perform normalization and model-based expression value calculations. Different microarray samples were normalized using the baseline array that has a median probe signal, and the PM-MM difference model was applied for expression value calculation. Genes were considered to be differentially regulated if they met all 3 of the following criteria: 1) the fold change between knockout and wild-type mice exceeds 1.2; 2) the threshold for the absolute difference between knockout vs. wild type means is larger than 50; 3) the p-value of unpaired t-test is less than 0.05.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tao,,Huang
| | Sample_contact_email | t0huan02@louisville.edu
| | Sample_contact_phone | 5028523059
| | Sample_contact_fax | 5028526228
| | Sample_contact_department | Anatomical Sciences and Neurobiology
| | Sample_contact_institute | University of Louisville
| | Sample_contact_address | 500 S Preston St, Rm1004
| | Sample_contact_city | Louisville
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1089nnn/GSM1089801/suppl/GSM1089801_NT4WT1.CEL.gz
| | Sample_series_id | GSE44734
| | Sample_data_row_count | 45101
| | |
|
GSM1089802 | GPL1261 |
|
NT4 Wild Type, biological rep2
|
Wild-type geniculate ganglion at E14.5
|
tissue: geniculate ganglion
genotype: NT4 wild type
age: Embryonic day 14.5
background mouse strain: 129
|
Gene expression data from NT4 wild-type geniculate ganglia at E14.5
|
| Sample_geo_accession | GSM1089802
| | Sample_status | Public on Feb 28 2013
| | Sample_submission_date | Feb 27 2013
| | Sample_last_update_date | Feb 28 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from geniculate ganglion using RNeasy micro kit. DNase I treatment was applied to eliminate traces of DNA during the procedure.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified, fragmented, and biotinylated using WT-Ovation Pico RNA Amplification System.
| | Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Mouse Genome 430 2.0 arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | dChip software was used to perform normalization and model-based expression value calculations. Different microarray samples were normalized using the baseline array that has a median probe signal, and the PM-MM difference model was applied for expression value calculation. Genes were considered to be differentially regulated if they met all 3 of the following criteria: 1) the fold change between knockout and wild-type mice exceeds 1.2; 2) the threshold for the absolute difference between knockout vs. wild type means is larger than 50; 3) the p-value of unpaired t-test is less than 0.05.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tao,,Huang
| | Sample_contact_email | t0huan02@louisville.edu
| | Sample_contact_phone | 5028523059
| | Sample_contact_fax | 5028526228
| | Sample_contact_department | Anatomical Sciences and Neurobiology
| | Sample_contact_institute | University of Louisville
| | Sample_contact_address | 500 S Preston St, Rm1004
| | Sample_contact_city | Louisville
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1089nnn/GSM1089802/suppl/GSM1089802_NT4WT2.CEL.gz
| | Sample_series_id | GSE44734
| | Sample_data_row_count | 45101
| | |
|
GSM1089803 | GPL1261 |
|
NT4 Wild Type, biological rep3
|
Wild-type geniculate ganglion at E14.5
|
tissue: geniculate ganglion
genotype: NT4 wild type
age: Embryonic day 14.5
background mouse strain: 129
|
Gene expression data from NT4 wild-type geniculate ganglia at E14.5
|
| Sample_geo_accession | GSM1089803
| | Sample_status | Public on Feb 28 2013
| | Sample_submission_date | Feb 27 2013
| | Sample_last_update_date | Feb 28 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from geniculate ganglion using RNeasy micro kit. DNase I treatment was applied to eliminate traces of DNA during the procedure.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified, fragmented, and biotinylated using WT-Ovation Pico RNA Amplification System.
| | Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Mouse Genome 430 2.0 arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | dChip software was used to perform normalization and model-based expression value calculations. Different microarray samples were normalized using the baseline array that has a median probe signal, and the PM-MM difference model was applied for expression value calculation. Genes were considered to be differentially regulated if they met all 3 of the following criteria: 1) the fold change between knockout and wild-type mice exceeds 1.2; 2) the threshold for the absolute difference between knockout vs. wild type means is larger than 50; 3) the p-value of unpaired t-test is less than 0.05.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tao,,Huang
| | Sample_contact_email | t0huan02@louisville.edu
| | Sample_contact_phone | 5028523059
| | Sample_contact_fax | 5028526228
| | Sample_contact_department | Anatomical Sciences and Neurobiology
| | Sample_contact_institute | University of Louisville
| | Sample_contact_address | 500 S Preston St, Rm1004
| | Sample_contact_city | Louisville
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1089nnn/GSM1089803/suppl/GSM1089803_NT4WT3.CEL.gz
| | Sample_series_id | GSE44734
| | Sample_data_row_count | 45101
| | |
|
GSM1089804 | GPL1261 |
|
NT4 Knockout, biological rep1
|
NT4 knockout geniculate ganglion at E14.5
|
tissue: geniculate ganglion
genotype: NT4 knockout
age: Embryonic day 14.5
background mouse strain: 129
|
Gene expression data from NT4 mutant geniculate ganglia at E14.5
|
| Sample_geo_accession | GSM1089804
| | Sample_status | Public on Feb 28 2013
| | Sample_submission_date | Feb 27 2013
| | Sample_last_update_date | Feb 28 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from geniculate ganglion using RNeasy micro kit. DNase I treatment was applied to eliminate traces of DNA during the procedure.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified, fragmented, and biotinylated using WT-Ovation Pico RNA Amplification System.
| | Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Mouse Genome 430 2.0 arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | dChip software was used to perform normalization and model-based expression value calculations. Different microarray samples were normalized using the baseline array that has a median probe signal, and the PM-MM difference model was applied for expression value calculation. Genes were considered to be differentially regulated if they met all 3 of the following criteria: 1) the fold change between knockout and wild-type mice exceeds 1.2; 2) the threshold for the absolute difference between knockout vs. wild type means is larger than 50; 3) the p-value of unpaired t-test is less than 0.05.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tao,,Huang
| | Sample_contact_email | t0huan02@louisville.edu
| | Sample_contact_phone | 5028523059
| | Sample_contact_fax | 5028526228
| | Sample_contact_department | Anatomical Sciences and Neurobiology
| | Sample_contact_institute | University of Louisville
| | Sample_contact_address | 500 S Preston St, Rm1004
| | Sample_contact_city | Louisville
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1089nnn/GSM1089804/suppl/GSM1089804_NT4KO1.CEL.gz
| | Sample_series_id | GSE44734
| | Sample_data_row_count | 45101
| | |
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GSM1089805 | GPL1261 |
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NT4 Knockout, biological rep2
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NT4 knockout geniculate ganglion at E14.5
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tissue: geniculate ganglion
genotype: NT4 knockout
age: Embryonic day 14.5
background mouse strain: 129
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Gene expression data from NT4 mutant geniculate ganglia at E14.5
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| Sample_geo_accession | GSM1089805
| | Sample_status | Public on Feb 28 2013
| | Sample_submission_date | Feb 27 2013
| | Sample_last_update_date | Feb 28 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from geniculate ganglion using RNeasy micro kit. DNase I treatment was applied to eliminate traces of DNA during the procedure.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified, fragmented, and biotinylated using WT-Ovation Pico RNA Amplification System.
| | Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Mouse Genome 430 2.0 arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | dChip software was used to perform normalization and model-based expression value calculations. Different microarray samples were normalized using the baseline array that has a median probe signal, and the PM-MM difference model was applied for expression value calculation. Genes were considered to be differentially regulated if they met all 3 of the following criteria: 1) the fold change between knockout and wild-type mice exceeds 1.2; 2) the threshold for the absolute difference between knockout vs. wild type means is larger than 50; 3) the p-value of unpaired t-test is less than 0.05.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tao,,Huang
| | Sample_contact_email | t0huan02@louisville.edu
| | Sample_contact_phone | 5028523059
| | Sample_contact_fax | 5028526228
| | Sample_contact_department | Anatomical Sciences and Neurobiology
| | Sample_contact_institute | University of Louisville
| | Sample_contact_address | 500 S Preston St, Rm1004
| | Sample_contact_city | Louisville
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1089nnn/GSM1089805/suppl/GSM1089805_NT4KO2.CEL.gz
| | Sample_series_id | GSE44734
| | Sample_data_row_count | 45101
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GSM1089806 | GPL1261 |
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NT4 Knockout, biological rep3
|
NT4 knockout geniculate ganglion at E14.5
|
tissue: geniculate ganglion
genotype: NT4 knockout
age: Embryonic day 14.5
background mouse strain: 129
|
Gene expression data from NT4 mutant geniculate ganglia at E14.5
|
| Sample_geo_accession | GSM1089806
| | Sample_status | Public on Feb 28 2013
| | Sample_submission_date | Feb 27 2013
| | Sample_last_update_date | Feb 28 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from geniculate ganglion using RNeasy micro kit. DNase I treatment was applied to eliminate traces of DNA during the procedure.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified, fragmented, and biotinylated using WT-Ovation Pico RNA Amplification System.
| | Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Mouse Genome 430 2.0 arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | dChip software was used to perform normalization and model-based expression value calculations. Different microarray samples were normalized using the baseline array that has a median probe signal, and the PM-MM difference model was applied for expression value calculation. Genes were considered to be differentially regulated if they met all 3 of the following criteria: 1) the fold change between knockout and wild-type mice exceeds 1.2; 2) the threshold for the absolute difference between knockout vs. wild type means is larger than 50; 3) the p-value of unpaired t-test is less than 0.05.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tao,,Huang
| | Sample_contact_email | t0huan02@louisville.edu
| | Sample_contact_phone | 5028523059
| | Sample_contact_fax | 5028526228
| | Sample_contact_department | Anatomical Sciences and Neurobiology
| | Sample_contact_institute | University of Louisville
| | Sample_contact_address | 500 S Preston St, Rm1004
| | Sample_contact_city | Louisville
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1089nnn/GSM1089806/suppl/GSM1089806_NT4KO3.CEL.gz
| | Sample_series_id | GSE44734
| | Sample_data_row_count | 45101
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