Search results for the GEO ID: GSE44807 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1091965 | GPL570 |
|
EGFP cells induced with Dox, biological rep1
|
Lentivirus transfected cells expressing EGFP after exposure to Dox from ALI D7-14
|
cell type: P0 primary HBE cells
treatment: EGFP lentivirus infected
donors: Donor 1
|
Gene expression data from cells expressed EGFP
|
Sample_geo_accession | GSM1091965
| Sample_status | Public on May 08 2013
| Sample_submission_date | Mar 03 2013
| Sample_last_update_date | May 08 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Dox was added to induce EGFP and DN-GRHL2 expression when cells were confluent. 7 days after induction, cells were collected.
| Sample_growth_protocol_ch1 | P0 primary HBE cells were infected with EGFP or DN-GRHL2 lentivirus and selected with puromycin. Cells were cultured at the air liquid interface and became confluent.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Micro kit (Qiagen) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA.
| Sample_hyb_protocol | Targets were hybridized to GeneChip® human genome U133 Plus 2.0 arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned with Affymetrix GeneChip® Scanner 7G and AGCC software version 1.1
| Sample_data_processing | Affymetrix Expression Console version 1.1 was used to generate the .cel files. Partek Genomics Suite 6.5 (Partek Inc., St. Louis, MO) was used to perform data analysis. Robust multi-chip analysis (RMA) normalization was done on the entire data set.
| Sample_platform_id | GPL570
| Sample_contact_name | Xia,,Gao
| Sample_contact_email | xia.gao@dm.duke.edu
| Sample_contact_institute | Duke University
| Sample_contact_address | 353 Nanaline Duke Bldg. Research Dr.
| Sample_contact_city | Durham
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27710
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1091nnn/GSM1091965/suppl/GSM1091965_EGFPDox+-1.CEL.gz
| Sample_series_id | GSE44807
| Sample_series_id | GSE44809
| Sample_data_row_count | 54675
| |
|
GSM1091966 | GPL570 |
|
DN-GRHL2 cells induced with Dox, biological rep1
|
Lentivirus transfected cells expressing DN-GRHL2 after exposure to Dox from ALI D7-14
|
cell type: P0 primary HBE cells
treatment: DN-GRHL2 lentivirus infected
donors: Donor 1
|
Gene expression data from cells expressed DN-GRHL2
|
Sample_geo_accession | GSM1091966
| Sample_status | Public on May 08 2013
| Sample_submission_date | Mar 03 2013
| Sample_last_update_date | May 08 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Dox was added to induce EGFP and DN-GRHL2 expression when cells were confluent. 7 days after induction, cells were collected.
| Sample_growth_protocol_ch1 | P0 primary HBE cells were infected with EGFP or DN-GRHL2 lentivirus and selected with puromycin. Cells were cultured at the air liquid interface and became confluent.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Micro kit (Qiagen) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA.
| Sample_hyb_protocol | Targets were hybridized to GeneChip® human genome U133 Plus 2.0 arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned with Affymetrix GeneChip® Scanner 7G and AGCC software version 1.1
| Sample_data_processing | Affymetrix Expression Console version 1.1 was used to generate the .cel files. Partek Genomics Suite 6.5 (Partek Inc., St. Louis, MO) was used to perform data analysis. Robust multi-chip analysis (RMA) normalization was done on the entire data set.
| Sample_platform_id | GPL570
| Sample_contact_name | Xia,,Gao
| Sample_contact_email | xia.gao@dm.duke.edu
| Sample_contact_institute | Duke University
| Sample_contact_address | 353 Nanaline Duke Bldg. Research Dr.
| Sample_contact_city | Durham
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27710
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1091nnn/GSM1091966/suppl/GSM1091966_DNGrhlDox+-1.CEL.gz
| Sample_series_id | GSE44807
| Sample_series_id | GSE44809
| Sample_data_row_count | 54675
| |
|
GSM1091967 | GPL570 |
|
EGFP cells induced with Dox, biological rep2
|
Lentivirus transfected cells expressing EGFP after exposure to Dox from ALI D7-14
|
cell type: P0 primary HBE cells
treatment: EGFP lentivirus infected
donors: Donor 2
|
Gene expression data from cells expressed EGFP
|
Sample_geo_accession | GSM1091967
| Sample_status | Public on May 08 2013
| Sample_submission_date | Mar 03 2013
| Sample_last_update_date | May 08 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Dox was added to induce EGFP and DN-GRHL2 expression when cells were confluent. 7 days after induction, cells were collected.
| Sample_growth_protocol_ch1 | P0 primary HBE cells were infected with EGFP or DN-GRHL2 lentivirus and selected with puromycin. Cells were cultured at the air liquid interface and became confluent.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Micro kit (Qiagen) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA.
| Sample_hyb_protocol | Targets were hybridized to GeneChip® human genome U133 Plus 2.0 arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned with Affymetrix GeneChip® Scanner 7G and AGCC software version 1.1
| Sample_data_processing | Affymetrix Expression Console version 1.1 was used to generate the .cel files. Partek Genomics Suite 6.5 (Partek Inc., St. Louis, MO) was used to perform data analysis. Robust multi-chip analysis (RMA) normalization was done on the entire data set.
| Sample_platform_id | GPL570
| Sample_contact_name | Xia,,Gao
| Sample_contact_email | xia.gao@dm.duke.edu
| Sample_contact_institute | Duke University
| Sample_contact_address | 353 Nanaline Duke Bldg. Research Dr.
| Sample_contact_city | Durham
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27710
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1091nnn/GSM1091967/suppl/GSM1091967_EGFPDox+-2.CEL.gz
| Sample_series_id | GSE44807
| Sample_series_id | GSE44809
| Sample_data_row_count | 54675
| |
|
GSM1091968 | GPL570 |
|
DN-GRHL2 cells induced with Dox, biological rep2
|
Lentivirus transfected cells expressing DN-GRHL2 after exposure to Dox from ALI D7-14
|
cell type: P0 primary HBE cells
treatment: DN-GRHL2 lentivirus infected
donors: Donor 2
|
Gene expression data from cells expressed DN-GRHL2
|
Sample_geo_accession | GSM1091968
| Sample_status | Public on May 08 2013
| Sample_submission_date | Mar 03 2013
| Sample_last_update_date | May 08 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Dox was added to induce EGFP and DN-GRHL2 expression when cells were confluent. 7 days after induction, cells were collected.
| Sample_growth_protocol_ch1 | P0 primary HBE cells were infected with EGFP or DN-GRHL2 lentivirus and selected with puromycin. Cells were cultured at the air liquid interface and became confluent.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Micro kit (Qiagen) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA.
| Sample_hyb_protocol | Targets were hybridized to GeneChip® human genome U133 Plus 2.0 arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned with Affymetrix GeneChip® Scanner 7G and AGCC software version 1.1
| Sample_data_processing | Affymetrix Expression Console version 1.1 was used to generate the .cel files. Partek Genomics Suite 6.5 (Partek Inc., St. Louis, MO) was used to perform data analysis. Robust multi-chip analysis (RMA) normalization was done on the entire data set.
| Sample_platform_id | GPL570
| Sample_contact_name | Xia,,Gao
| Sample_contact_email | xia.gao@dm.duke.edu
| Sample_contact_institute | Duke University
| Sample_contact_address | 353 Nanaline Duke Bldg. Research Dr.
| Sample_contact_city | Durham
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27710
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1091nnn/GSM1091968/suppl/GSM1091968_DNGrhlDox+-2.CEL.gz
| Sample_series_id | GSE44807
| Sample_series_id | GSE44809
| Sample_data_row_count | 54675
| |
|
GSM1091969 | GPL570 |
|
EGFP cells induced with Dox, biological rep3
|
Lentivirus transfected cells expressing EGFP after exposure to Dox from ALI D7-14
|
cell type: P0 primary HBE cells
treatment: EGFP lentivirus infected
donors: Donor 3
|
Gene expression data from cells expressed EGFP
|
Sample_geo_accession | GSM1091969
| Sample_status | Public on May 08 2013
| Sample_submission_date | Mar 03 2013
| Sample_last_update_date | May 08 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Dox was added to induce EGFP and DN-GRHL2 expression when cells were confluent. 7 days after induction, cells were collected.
| Sample_growth_protocol_ch1 | P0 primary HBE cells were infected with EGFP or DN-GRHL2 lentivirus and selected with puromycin. Cells were cultured at the air liquid interface and became confluent.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Micro kit (Qiagen) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA.
| Sample_hyb_protocol | Targets were hybridized to GeneChip® human genome U133 Plus 2.0 arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned with Affymetrix GeneChip® Scanner 7G and AGCC software version 1.1
| Sample_data_processing | Affymetrix Expression Console version 1.1 was used to generate the .cel files. Partek Genomics Suite 6.5 (Partek Inc., St. Louis, MO) was used to perform data analysis. Robust multi-chip analysis (RMA) normalization was done on the entire data set.
| Sample_platform_id | GPL570
| Sample_contact_name | Xia,,Gao
| Sample_contact_email | xia.gao@dm.duke.edu
| Sample_contact_institute | Duke University
| Sample_contact_address | 353 Nanaline Duke Bldg. Research Dr.
| Sample_contact_city | Durham
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27710
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1091nnn/GSM1091969/suppl/GSM1091969_EGFPDox+-3.CEL.gz
| Sample_series_id | GSE44807
| Sample_series_id | GSE44809
| Sample_data_row_count | 54675
| |
|
GSM1091970 | GPL570 |
|
DN-GRHL2 cells induced with Dox, biological rep3
|
Lentivirus transfected cells expressing DN-GRHL2 after exposure to Dox from ALI D7-14
|
cell type: P0 primary HBE cells
treatment: DN-GRHL2 lentivirus infected
donors: Donor 3
|
Gene expression data from cells expressed DN-GRHL2
|
Sample_geo_accession | GSM1091970
| Sample_status | Public on May 08 2013
| Sample_submission_date | Mar 03 2013
| Sample_last_update_date | May 08 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Dox was added to induce EGFP and DN-GRHL2 expression when cells were confluent. 7 days after induction, cells were collected.
| Sample_growth_protocol_ch1 | P0 primary HBE cells were infected with EGFP or DN-GRHL2 lentivirus and selected with puromycin. Cells were cultured at the air liquid interface and became confluent.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Micro kit (Qiagen) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA.
| Sample_hyb_protocol | Targets were hybridized to GeneChip® human genome U133 Plus 2.0 arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned with Affymetrix GeneChip® Scanner 7G and AGCC software version 1.1
| Sample_data_processing | Affymetrix Expression Console version 1.1 was used to generate the .cel files. Partek Genomics Suite 6.5 (Partek Inc., St. Louis, MO) was used to perform data analysis. Robust multi-chip analysis (RMA) normalization was done on the entire data set.
| Sample_platform_id | GPL570
| Sample_contact_name | Xia,,Gao
| Sample_contact_email | xia.gao@dm.duke.edu
| Sample_contact_institute | Duke University
| Sample_contact_address | 353 Nanaline Duke Bldg. Research Dr.
| Sample_contact_city | Durham
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27710
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1091nnn/GSM1091970/suppl/GSM1091970_DNGrhlDox+-3.CEL.gz
| Sample_series_id | GSE44807
| Sample_series_id | GSE44809
| Sample_data_row_count | 54675
| |
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