Search results for the GEO ID: GSE44809  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1091965 | GPL570 |
|
EGFP cells induced with Dox, biological rep1
|
Lentivirus transfected cells expressing EGFP after exposure to Dox from ALI D7-14
|
cell type: P0 primary HBE cells
treatment: EGFP lentivirus infected
donors: Donor 1
|
Gene expression data from cells expressed EGFP
|
| Sample_geo_accession | GSM1091965
| | Sample_status | Public on May 08 2013
| | Sample_submission_date | Mar 03 2013
| | Sample_last_update_date | May 08 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Dox was added to induce EGFP and DN-GRHL2 expression when cells were confluent. 7 days after induction, cells were collected.
| | Sample_growth_protocol_ch1 | P0 primary HBE cells were infected with EGFP or DN-GRHL2 lentivirus and selected with puromycin. Cells were cultured at the air liquid interface and became confluent.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Micro kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA.
| | Sample_hyb_protocol | Targets were hybridized to GeneChip® human genome U133 Plus 2.0 arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned with Affymetrix GeneChip® Scanner 7G and AGCC software version 1.1
| | Sample_data_processing | Affymetrix Expression Console version 1.1 was used to generate the .cel files. Partek Genomics Suite 6.5 (Partek Inc., St. Louis, MO) was used to perform data analysis. Robust multi-chip analysis (RMA) normalization was done on the entire data set.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xia,,Gao
| | Sample_contact_email | xia.gao@dm.duke.edu
| | Sample_contact_institute | Duke University
| | Sample_contact_address | 353 Nanaline Duke Bldg. Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1091nnn/GSM1091965/suppl/GSM1091965_EGFPDox+-1.CEL.gz
| | Sample_series_id | GSE44807
| | Sample_series_id | GSE44809
| | Sample_data_row_count | 54675
| | |
|
GSM1091966 | GPL570 |
|
DN-GRHL2 cells induced with Dox, biological rep1
|
Lentivirus transfected cells expressing DN-GRHL2 after exposure to Dox from ALI D7-14
|
cell type: P0 primary HBE cells
treatment: DN-GRHL2 lentivirus infected
donors: Donor 1
|
Gene expression data from cells expressed DN-GRHL2
|
| Sample_geo_accession | GSM1091966
| | Sample_status | Public on May 08 2013
| | Sample_submission_date | Mar 03 2013
| | Sample_last_update_date | May 08 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Dox was added to induce EGFP and DN-GRHL2 expression when cells were confluent. 7 days after induction, cells were collected.
| | Sample_growth_protocol_ch1 | P0 primary HBE cells were infected with EGFP or DN-GRHL2 lentivirus and selected with puromycin. Cells were cultured at the air liquid interface and became confluent.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Micro kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA.
| | Sample_hyb_protocol | Targets were hybridized to GeneChip® human genome U133 Plus 2.0 arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned with Affymetrix GeneChip® Scanner 7G and AGCC software version 1.1
| | Sample_data_processing | Affymetrix Expression Console version 1.1 was used to generate the .cel files. Partek Genomics Suite 6.5 (Partek Inc., St. Louis, MO) was used to perform data analysis. Robust multi-chip analysis (RMA) normalization was done on the entire data set.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xia,,Gao
| | Sample_contact_email | xia.gao@dm.duke.edu
| | Sample_contact_institute | Duke University
| | Sample_contact_address | 353 Nanaline Duke Bldg. Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1091nnn/GSM1091966/suppl/GSM1091966_DNGrhlDox+-1.CEL.gz
| | Sample_series_id | GSE44807
| | Sample_series_id | GSE44809
| | Sample_data_row_count | 54675
| | |
|
GSM1091967 | GPL570 |
|
EGFP cells induced with Dox, biological rep2
|
Lentivirus transfected cells expressing EGFP after exposure to Dox from ALI D7-14
|
cell type: P0 primary HBE cells
treatment: EGFP lentivirus infected
donors: Donor 2
|
Gene expression data from cells expressed EGFP
|
| Sample_geo_accession | GSM1091967
| | Sample_status | Public on May 08 2013
| | Sample_submission_date | Mar 03 2013
| | Sample_last_update_date | May 08 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Dox was added to induce EGFP and DN-GRHL2 expression when cells were confluent. 7 days after induction, cells were collected.
| | Sample_growth_protocol_ch1 | P0 primary HBE cells were infected with EGFP or DN-GRHL2 lentivirus and selected with puromycin. Cells were cultured at the air liquid interface and became confluent.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Micro kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA.
| | Sample_hyb_protocol | Targets were hybridized to GeneChip® human genome U133 Plus 2.0 arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned with Affymetrix GeneChip® Scanner 7G and AGCC software version 1.1
| | Sample_data_processing | Affymetrix Expression Console version 1.1 was used to generate the .cel files. Partek Genomics Suite 6.5 (Partek Inc., St. Louis, MO) was used to perform data analysis. Robust multi-chip analysis (RMA) normalization was done on the entire data set.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xia,,Gao
| | Sample_contact_email | xia.gao@dm.duke.edu
| | Sample_contact_institute | Duke University
| | Sample_contact_address | 353 Nanaline Duke Bldg. Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1091nnn/GSM1091967/suppl/GSM1091967_EGFPDox+-2.CEL.gz
| | Sample_series_id | GSE44807
| | Sample_series_id | GSE44809
| | Sample_data_row_count | 54675
| | |
|
GSM1091968 | GPL570 |
|
DN-GRHL2 cells induced with Dox, biological rep2
|
Lentivirus transfected cells expressing DN-GRHL2 after exposure to Dox from ALI D7-14
|
cell type: P0 primary HBE cells
treatment: DN-GRHL2 lentivirus infected
donors: Donor 2
|
Gene expression data from cells expressed DN-GRHL2
|
| Sample_geo_accession | GSM1091968
| | Sample_status | Public on May 08 2013
| | Sample_submission_date | Mar 03 2013
| | Sample_last_update_date | May 08 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Dox was added to induce EGFP and DN-GRHL2 expression when cells were confluent. 7 days after induction, cells were collected.
| | Sample_growth_protocol_ch1 | P0 primary HBE cells were infected with EGFP or DN-GRHL2 lentivirus and selected with puromycin. Cells were cultured at the air liquid interface and became confluent.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Micro kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA.
| | Sample_hyb_protocol | Targets were hybridized to GeneChip® human genome U133 Plus 2.0 arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned with Affymetrix GeneChip® Scanner 7G and AGCC software version 1.1
| | Sample_data_processing | Affymetrix Expression Console version 1.1 was used to generate the .cel files. Partek Genomics Suite 6.5 (Partek Inc., St. Louis, MO) was used to perform data analysis. Robust multi-chip analysis (RMA) normalization was done on the entire data set.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xia,,Gao
| | Sample_contact_email | xia.gao@dm.duke.edu
| | Sample_contact_institute | Duke University
| | Sample_contact_address | 353 Nanaline Duke Bldg. Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1091nnn/GSM1091968/suppl/GSM1091968_DNGrhlDox+-2.CEL.gz
| | Sample_series_id | GSE44807
| | Sample_series_id | GSE44809
| | Sample_data_row_count | 54675
| | |
|
GSM1091969 | GPL570 |
|
EGFP cells induced with Dox, biological rep3
|
Lentivirus transfected cells expressing EGFP after exposure to Dox from ALI D7-14
|
cell type: P0 primary HBE cells
treatment: EGFP lentivirus infected
donors: Donor 3
|
Gene expression data from cells expressed EGFP
|
| Sample_geo_accession | GSM1091969
| | Sample_status | Public on May 08 2013
| | Sample_submission_date | Mar 03 2013
| | Sample_last_update_date | May 08 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Dox was added to induce EGFP and DN-GRHL2 expression when cells were confluent. 7 days after induction, cells were collected.
| | Sample_growth_protocol_ch1 | P0 primary HBE cells were infected with EGFP or DN-GRHL2 lentivirus and selected with puromycin. Cells were cultured at the air liquid interface and became confluent.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Micro kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA.
| | Sample_hyb_protocol | Targets were hybridized to GeneChip® human genome U133 Plus 2.0 arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned with Affymetrix GeneChip® Scanner 7G and AGCC software version 1.1
| | Sample_data_processing | Affymetrix Expression Console version 1.1 was used to generate the .cel files. Partek Genomics Suite 6.5 (Partek Inc., St. Louis, MO) was used to perform data analysis. Robust multi-chip analysis (RMA) normalization was done on the entire data set.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xia,,Gao
| | Sample_contact_email | xia.gao@dm.duke.edu
| | Sample_contact_institute | Duke University
| | Sample_contact_address | 353 Nanaline Duke Bldg. Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1091nnn/GSM1091969/suppl/GSM1091969_EGFPDox+-3.CEL.gz
| | Sample_series_id | GSE44807
| | Sample_series_id | GSE44809
| | Sample_data_row_count | 54675
| | |
|
GSM1091970 | GPL570 |
|
DN-GRHL2 cells induced with Dox, biological rep3
|
Lentivirus transfected cells expressing DN-GRHL2 after exposure to Dox from ALI D7-14
|
cell type: P0 primary HBE cells
treatment: DN-GRHL2 lentivirus infected
donors: Donor 3
|
Gene expression data from cells expressed DN-GRHL2
|
| Sample_geo_accession | GSM1091970
| | Sample_status | Public on May 08 2013
| | Sample_submission_date | Mar 03 2013
| | Sample_last_update_date | May 08 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Dox was added to induce EGFP and DN-GRHL2 expression when cells were confluent. 7 days after induction, cells were collected.
| | Sample_growth_protocol_ch1 | P0 primary HBE cells were infected with EGFP or DN-GRHL2 lentivirus and selected with puromycin. Cells were cultured at the air liquid interface and became confluent.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Micro kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA.
| | Sample_hyb_protocol | Targets were hybridized to GeneChip® human genome U133 Plus 2.0 arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned with Affymetrix GeneChip® Scanner 7G and AGCC software version 1.1
| | Sample_data_processing | Affymetrix Expression Console version 1.1 was used to generate the .cel files. Partek Genomics Suite 6.5 (Partek Inc., St. Louis, MO) was used to perform data analysis. Robust multi-chip analysis (RMA) normalization was done on the entire data set.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xia,,Gao
| | Sample_contact_email | xia.gao@dm.duke.edu
| | Sample_contact_institute | Duke University
| | Sample_contact_address | 353 Nanaline Duke Bldg. Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1091nnn/GSM1091970/suppl/GSM1091970_DNGrhlDox+-3.CEL.gz
| | Sample_series_id | GSE44807
| | Sample_series_id | GSE44809
| | Sample_data_row_count | 54675
| | |
|
GSM1091971 | GPL570 |
|
H2B-GFP cells induced with Dox, biological rep 1
|
Lentivirus transfected cells expressing H2B-GFP after exposure to Dox from ALI D17-19
|
cell type: P0 primary HBE cells
treatment: H2B-GFP lentivirus infected
donors: Donor 1
|
Gene expression data from cells expressed H2B-GFP
|
| Sample_geo_accession | GSM1091971
| | Sample_status | Public on May 08 2013
| | Sample_submission_date | Mar 03 2013
| | Sample_last_update_date | May 08 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Dox was added for 48h to induce EGFP and DN-GRHL2 expression when TER reached a threshold level.
| | Sample_growth_protocol_ch1 | P0 primary HBE cells were infected with EGFP or DN-GRHL2 lentivirus and selected with puromycin. Cells cultured in air liquid interface began to increase TER and reached a plateau between ALI D14 and 18, depending on the donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Micro kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA.
| | Sample_hyb_protocol | Targets were hybridized to GeneChip® human genome U133 Plus 2.0 arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned with Affymetrix GeneChip® Scanner 7G and AGCC software version 1.1
| | Sample_data_processing | Affymetrix Expression Console version 1.1 was used to generate the .cel files. Partek Genomics Suite 6.5 (Partek Inc., St. Louis, MO) was used to perform data analysis. Robust multi-chip analysis (RMA) normalization was done on the entire data set.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xia,,Gao
| | Sample_contact_email | xia.gao@dm.duke.edu
| | Sample_contact_institute | Duke University
| | Sample_contact_address | 353 Nanaline Duke Bldg. Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1091nnn/GSM1091971/suppl/GSM1091971_H2B-GFPDox+-1.CEL.gz
| | Sample_series_id | GSE44808
| | Sample_series_id | GSE44809
| | Sample_data_row_count | 54675
| | |
|
GSM1091972 | GPL570 |
|
DN-GRHL2 cells induced with Dox, biological rep 1
|
Lentivirus transfected cells expressing DN-GRHL2 after exposure to Dox from ALI D17-19
|
cell type: P0 primary HBE cells
treatment: DN-GRHL2 lentivirus infected
donors: Donor 1
|
Gene expression data from cells expressed DN-GRHL2
|
| Sample_geo_accession | GSM1091972
| | Sample_status | Public on May 08 2013
| | Sample_submission_date | Mar 03 2013
| | Sample_last_update_date | May 08 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Dox was added for 48h to induce EGFP and DN-GRHL2 expression when TER reached a threshold level.
| | Sample_growth_protocol_ch1 | P0 primary HBE cells were infected with EGFP or DN-GRHL2 lentivirus and selected with puromycin. Cells cultured in air liquid interface began to increase TER and reached a plateau between ALI D14 and 18, depending on the donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Micro kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA.
| | Sample_hyb_protocol | Targets were hybridized to GeneChip® human genome U133 Plus 2.0 arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned with Affymetrix GeneChip® Scanner 7G and AGCC software version 1.1
| | Sample_data_processing | Affymetrix Expression Console version 1.1 was used to generate the .cel files. Partek Genomics Suite 6.5 (Partek Inc., St. Louis, MO) was used to perform data analysis. Robust multi-chip analysis (RMA) normalization was done on the entire data set.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xia,,Gao
| | Sample_contact_email | xia.gao@dm.duke.edu
| | Sample_contact_institute | Duke University
| | Sample_contact_address | 353 Nanaline Duke Bldg. Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1091nnn/GSM1091972/suppl/GSM1091972_DNGrhlDox+-1.CEL.gz
| | Sample_series_id | GSE44808
| | Sample_series_id | GSE44809
| | Sample_data_row_count | 54675
| | |
|
GSM1091973 | GPL570 |
|
H2B-GFP cells induced with Dox, biological rep 2
|
Lentivirus transfected cells expressing H2B-GFP after exposure to Dox from ALI D14-16
|
cell type: P0 primary HBE cells
treatment: H2B-GFP lentivirus infected
donors: Donor 2
|
Gene expression data from cells expressed H2B-GFP
|
| Sample_geo_accession | GSM1091973
| | Sample_status | Public on May 08 2013
| | Sample_submission_date | Mar 03 2013
| | Sample_last_update_date | May 08 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Dox was added for 48h to induce EGFP and DN-GRHL2 expression when TER reached a threshold level.
| | Sample_growth_protocol_ch1 | P0 primary HBE cells were infected with EGFP or DN-GRHL2 lentivirus and selected with puromycin. Cells cultured in air liquid interface began to increase TER and reached a plateau between ALI D14 and 18, depending on the donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Micro kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA.
| | Sample_hyb_protocol | Targets were hybridized to GeneChip® human genome U133 Plus 2.0 arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned with Affymetrix GeneChip® Scanner 7G and AGCC software version 1.1
| | Sample_data_processing | Affymetrix Expression Console version 1.1 was used to generate the .cel files. Partek Genomics Suite 6.5 (Partek Inc., St. Louis, MO) was used to perform data analysis. Robust multi-chip analysis (RMA) normalization was done on the entire data set.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xia,,Gao
| | Sample_contact_email | xia.gao@dm.duke.edu
| | Sample_contact_institute | Duke University
| | Sample_contact_address | 353 Nanaline Duke Bldg. Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1091nnn/GSM1091973/suppl/GSM1091973_H2B-GFPDox+-2.CEL.gz
| | Sample_series_id | GSE44808
| | Sample_series_id | GSE44809
| | Sample_data_row_count | 54675
| | |
|
GSM1091974 | GPL570 |
|
DN-GRHL2 cells induced with Dox, biological rep 2
|
Lentivirus transfected cells expressing DN-GRHL2 after exposure to Dox from ALI D14-16
|
cell type: P0 primary HBE cells
treatment: DN-GRHL2 lentivirus infected
donors: Donor 2
|
Gene expression data from cells expressed DN-GRHL2
|
| Sample_geo_accession | GSM1091974
| | Sample_status | Public on May 08 2013
| | Sample_submission_date | Mar 03 2013
| | Sample_last_update_date | May 08 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Dox was added for 48h to induce EGFP and DN-GRHL2 expression when TER reached a threshold level.
| | Sample_growth_protocol_ch1 | P0 primary HBE cells were infected with EGFP or DN-GRHL2 lentivirus and selected with puromycin. Cells cultured in air liquid interface began to increase TER and reached a plateau between ALI D14 and 18, depending on the donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Micro kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA.
| | Sample_hyb_protocol | Targets were hybridized to GeneChip® human genome U133 Plus 2.0 arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned with Affymetrix GeneChip® Scanner 7G and AGCC software version 1.1
| | Sample_data_processing | Affymetrix Expression Console version 1.1 was used to generate the .cel files. Partek Genomics Suite 6.5 (Partek Inc., St. Louis, MO) was used to perform data analysis. Robust multi-chip analysis (RMA) normalization was done on the entire data set.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xia,,Gao
| | Sample_contact_email | xia.gao@dm.duke.edu
| | Sample_contact_institute | Duke University
| | Sample_contact_address | 353 Nanaline Duke Bldg. Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1091nnn/GSM1091974/suppl/GSM1091974_DNGrhlDox+-2.CEL.gz
| | Sample_series_id | GSE44808
| | Sample_series_id | GSE44809
| | Sample_data_row_count | 54675
| | |
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GSM1091975 | GPL570 |
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H2B-GFP cells induced with Dox, biological rep 3
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Lentivirus transfected cells expressing H2B-GFP after exposure to Dox from ALI D18-20
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cell type: P0 primary HBE cells
treatment: H2B-GFP lentivirus infected
donors: Donor 3
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Gene expression data from cells expressed H2B-GFP
|
| Sample_geo_accession | GSM1091975
| | Sample_status | Public on May 08 2013
| | Sample_submission_date | Mar 03 2013
| | Sample_last_update_date | May 08 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Dox was added for 48h to induce EGFP and DN-GRHL2 expression when TER reached a threshold level.
| | Sample_growth_protocol_ch1 | P0 primary HBE cells were infected with EGFP or DN-GRHL2 lentivirus and selected with puromycin. Cells cultured in air liquid interface began to increase TER and reached a plateau between ALI D14 and 18, depending on the donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Micro kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA.
| | Sample_hyb_protocol | Targets were hybridized to GeneChip® human genome U133 Plus 2.0 arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned with Affymetrix GeneChip® Scanner 7G and AGCC software version 1.1
| | Sample_data_processing | Affymetrix Expression Console version 1.1 was used to generate the .cel files. Partek Genomics Suite 6.5 (Partek Inc., St. Louis, MO) was used to perform data analysis. Robust multi-chip analysis (RMA) normalization was done on the entire data set.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xia,,Gao
| | Sample_contact_email | xia.gao@dm.duke.edu
| | Sample_contact_institute | Duke University
| | Sample_contact_address | 353 Nanaline Duke Bldg. Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1091nnn/GSM1091975/suppl/GSM1091975_H2B-GFPDox+-3.CEL.gz
| | Sample_series_id | GSE44808
| | Sample_series_id | GSE44809
| | Sample_data_row_count | 54675
| | |
|
GSM1091976 | GPL570 |
|
DN-GRHL2 cells induced with Dox, biological rep 3
|
Lentivirus transfected cells expressing DN-GRHL2 after exposure to Dox from ALI D18-20
|
cell type: P0 primary HBE cells
treatment: DN-GRHL2 lentivirus infected
donors: Donor 3
|
Gene expression data from cells expressed DN-GRHL2
|
| Sample_geo_accession | GSM1091976
| | Sample_status | Public on May 08 2013
| | Sample_submission_date | Mar 03 2013
| | Sample_last_update_date | May 08 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Dox was added for 48h to induce EGFP and DN-GRHL2 expression when TER reached a threshold level.
| | Sample_growth_protocol_ch1 | P0 primary HBE cells were infected with EGFP or DN-GRHL2 lentivirus and selected with puromycin. Cells cultured in air liquid interface began to increase TER and reached a plateau between ALI D14 and 18, depending on the donors.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Micro kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA.
| | Sample_hyb_protocol | Targets were hybridized to GeneChip® human genome U133 Plus 2.0 arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned with Affymetrix GeneChip® Scanner 7G and AGCC software version 1.1
| | Sample_data_processing | Affymetrix Expression Console version 1.1 was used to generate the .cel files. Partek Genomics Suite 6.5 (Partek Inc., St. Louis, MO) was used to perform data analysis. Robust multi-chip analysis (RMA) normalization was done on the entire data set.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xia,,Gao
| | Sample_contact_email | xia.gao@dm.duke.edu
| | Sample_contact_institute | Duke University
| | Sample_contact_address | 353 Nanaline Duke Bldg. Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1091nnn/GSM1091976/suppl/GSM1091976_DNGrhlDox+-3.CEL.gz
| | Sample_series_id | GSE44808
| | Sample_series_id | GSE44809
| | Sample_data_row_count | 54675
| | |
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