Result

Search results for the GEO ID: GSE44841

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM1092481

GPL570

GBM_neurosphere_4days_1

Glioblastoma multiforme cells, neurospheres, 4 days

tissue: Brain disease status: Glioblastoma multiforme patient: G48 cell type: neurospheres

reanalysis of GSM520522

Sample_geo_accession	GSM1092481
Sample_status	Public on Mar 05 2013
Sample_submission_date	Mar 04 2013
Sample_last_update_date	Mar 05 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Glioblastoma neurospheres were differentiated in the presence of 10% fetal calf serum and in absence of B-27 supplement for 4days
Sample_growth_protocol_ch1	Tumor cells were resuspended in serum-free DMEM/F12 medium (Invitrogen, Carlsbad, CA) containing human recombinant EGF (20 ng/ml; Sigma, St. Louis, MO), bFGF (20 ng/ml; Sigma), B-27 (20 ml per ml of medium; Invitrogen) and heparin (2 mg/ml), and seeded at a density of 3x106 live cells/60-mm plate
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	The cDNA was prepared from 5 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
Sample_hyb_protocol	Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133A 2.0 chip according to the protocols provided by the manufacturer.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
Sample_data_processing	Data analysis performed using R (2.15.0) and Bioconductor packages. The function rma of package affy (1.36.0) was used for normalization
Sample_platform_id	GPL570
Sample_contact_name	Raquel,,Malumbres
Sample_contact_email	rmalumbres@yahoo.com
Sample_contact_phone	+34 948194700
Sample_contact_laboratory	Molecular Oncology
Sample_contact_department	Oncology
Sample_contact_institute	University of Navarra/ Center for Applied Medical Research
Sample_contact_address	Avenida Pio XII 55
Sample_contact_city	Pamplona
Sample_contact_state	Navarra
Sample_contact_zip/postal_code	31008
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1092nnn/GSM1092481/suppl/GSM1092481_G48_NS_12209.CEL.gz
Sample_relation	Reanalysis of: GSM520522
Sample_series_id	GSE44841
Sample_series_id	GSE44843
Sample_data_row_count	54675

GSM1092482

GPL570

GBM_differentiation_4days_1

Glioblastoma multiforme cells, differentiation, 4 days

tissue: Brain disease status: Glioblastoma multiforme patient: G48 cell type: differentiated 4 days

reanalysis of GSM520521

Sample_geo_accession	GSM1092482
Sample_status	Public on Mar 05 2013
Sample_submission_date	Mar 04 2013
Sample_last_update_date	Mar 05 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Glioblastoma neurospheres were differentiated in the presence of 10% fetal calf serum and in absence of B-27 supplement for 4days
Sample_growth_protocol_ch1	Tumor cells were resuspended in serum-free DMEM/F12 medium (Invitrogen, Carlsbad, CA) containing human recombinant EGF (20 ng/ml; Sigma, St. Louis, MO), bFGF (20 ng/ml; Sigma), B-27 (20 ml per ml of medium; Invitrogen) and heparin (2 mg/ml), and seeded at a density of 3x106 live cells/60-mm plate
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	The cDNA was prepared from 5 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
Sample_hyb_protocol	Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133A 2.0 chip according to the protocols provided by the manufacturer.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
Sample_data_processing	Data analysis performed using R (2.15.0) and Bioconductor packages. The function rma of package affy (1.36.0) was used for normalization
Sample_platform_id	GPL570
Sample_contact_name	Raquel,,Malumbres
Sample_contact_email	rmalumbres@yahoo.com
Sample_contact_phone	+34 948194700
Sample_contact_laboratory	Molecular Oncology
Sample_contact_department	Oncology
Sample_contact_institute	University of Navarra/ Center for Applied Medical Research
Sample_contact_address	Avenida Pio XII 55
Sample_contact_city	Pamplona
Sample_contact_state	Navarra
Sample_contact_zip/postal_code	31008
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1092nnn/GSM1092482/suppl/GSM1092482_G48_DF_4d_12209.CEL.gz
Sample_relation	Reanalysis of: GSM520521
Sample_series_id	GSE44841
Sample_series_id	GSE44843
Sample_data_row_count	54675

GSM1092483

GPL570

GBM_neurosphere_4days_2

Glioblastoma multiforme cells, neurospheres, 4 days

tissue: Brain disease status: Glioblastoma multiforme patient: G52 cell type: neurospheres

reanalysis of GSM520524

Sample_geo_accession	GSM1092483
Sample_status	Public on Mar 05 2013
Sample_submission_date	Mar 04 2013
Sample_last_update_date	Mar 05 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Glioblastoma neurospheres were differentiated in the presence of 10% fetal calf serum and in absence of B-27 supplement for 4days
Sample_growth_protocol_ch1	Tumor cells were resuspended in serum-free DMEM/F12 medium (Invitrogen, Carlsbad, CA) containing human recombinant EGF (20 ng/ml; Sigma, St. Louis, MO), bFGF (20 ng/ml; Sigma), B-27 (20 ml per ml of medium; Invitrogen) and heparin (2 mg/ml), and seeded at a density of 3x106 live cells/60-mm plate
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	The cDNA was prepared from 5 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
Sample_hyb_protocol	Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133A 2.0 chip according to the protocols provided by the manufacturer.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
Sample_data_processing	Data analysis performed using R (2.15.0) and Bioconductor packages. The function rma of package affy (1.36.0) was used for normalization
Sample_platform_id	GPL570
Sample_contact_name	Raquel,,Malumbres
Sample_contact_email	rmalumbres@yahoo.com
Sample_contact_phone	+34 948194700
Sample_contact_laboratory	Molecular Oncology
Sample_contact_department	Oncology
Sample_contact_institute	University of Navarra/ Center for Applied Medical Research
Sample_contact_address	Avenida Pio XII 55
Sample_contact_city	Pamplona
Sample_contact_state	Navarra
Sample_contact_zip/postal_code	31008
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1092nnn/GSM1092483/suppl/GSM1092483_G52_NS_081008.CEL.gz
Sample_relation	Reanalysis of: GSM520524
Sample_series_id	GSE44841
Sample_series_id	GSE44843
Sample_data_row_count	54675

GSM1092484

GPL570

GBM_differentiation_4days_2

Glioblastoma multiforme cells, differentiation, 4 days

tissue: Brain disease status: Glioblastoma multiforme patient: G52 cell type: differentiated 4 days

reanalysis of GSM520523

Sample_geo_accession	GSM1092484
Sample_status	Public on Mar 05 2013
Sample_submission_date	Mar 04 2013
Sample_last_update_date	Mar 05 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Glioblastoma neurospheres were differentiated in the presence of 10% fetal calf serum and in absence of B-27 supplement for 4days
Sample_growth_protocol_ch1	Tumor cells were resuspended in serum-free DMEM/F12 medium (Invitrogen, Carlsbad, CA) containing human recombinant EGF (20 ng/ml; Sigma, St. Louis, MO), bFGF (20 ng/ml; Sigma), B-27 (20 ml per ml of medium; Invitrogen) and heparin (2 mg/ml), and seeded at a density of 3x106 live cells/60-mm plate
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	The cDNA was prepared from 5 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
Sample_hyb_protocol	Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133A 2.0 chip according to the protocols provided by the manufacturer.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
Sample_data_processing	Data analysis performed using R (2.15.0) and Bioconductor packages. The function rma of package affy (1.36.0) was used for normalization
Sample_platform_id	GPL570
Sample_contact_name	Raquel,,Malumbres
Sample_contact_email	rmalumbres@yahoo.com
Sample_contact_phone	+34 948194700
Sample_contact_laboratory	Molecular Oncology
Sample_contact_department	Oncology
Sample_contact_institute	University of Navarra/ Center for Applied Medical Research
Sample_contact_address	Avenida Pio XII 55
Sample_contact_city	Pamplona
Sample_contact_state	Navarra
Sample_contact_zip/postal_code	31008
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1092nnn/GSM1092484/suppl/GSM1092484_G52_DF_4d_081008.CEL.gz
Sample_relation	Reanalysis of: GSM520523
Sample_series_id	GSE44841
Sample_series_id	GSE44843
Sample_data_row_count	54675

GSM1092485

GPL570

GBM_neurosphere_4days_3

Glioblastoma multiforme cells, neurospheres, 4 days

tissue: Brain disease status: Glioblastoma multiforme patient: G59 cell type: neurospheres

Sample_geo_accession	GSM1092485
Sample_status	Public on Mar 05 2013
Sample_submission_date	Mar 04 2013
Sample_last_update_date	Mar 05 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Glioblastoma neurospheres were differentiated in the presence of 10% fetal calf serum and in absence of B-27 supplement for 4days
Sample_growth_protocol_ch1	Tumor cells were resuspended in serum-free DMEM/F12 medium (Invitrogen, Carlsbad, CA) containing human recombinant EGF (20 ng/ml; Sigma, St. Louis, MO), bFGF (20 ng/ml; Sigma), B-27 (20 ml per ml of medium; Invitrogen) and heparin (2 mg/ml), and seeded at a density of 3x106 live cells/60-mm plate
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	The cDNA was prepared from 5 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
Sample_hyb_protocol	Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133A 2.0 chip according to the protocols provided by the manufacturer.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
Sample_data_processing	Data analysis performed using R (2.15.0) and Bioconductor packages. The function rma of package affy (1.36.0) was used for normalization
Sample_platform_id	GPL570
Sample_contact_name	Raquel,,Malumbres
Sample_contact_email	rmalumbres@yahoo.com
Sample_contact_phone	+34 948194700
Sample_contact_laboratory	Molecular Oncology
Sample_contact_department	Oncology
Sample_contact_institute	University of Navarra/ Center for Applied Medical Research
Sample_contact_address	Avenida Pio XII 55
Sample_contact_city	Pamplona
Sample_contact_state	Navarra
Sample_contact_zip/postal_code	31008
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1092nnn/GSM1092485/suppl/GSM1092485_G59_NS_12209.CEL.gz
Sample_series_id	GSE44841
Sample_series_id	GSE44843
Sample_data_row_count	54675

GSM1092486

GPL570

GBM_differentiation_4days_3

Glioblastoma multiforme cells, differentiation, 4 days

tissue: Brain disease status: Glioblastoma multiforme patient: G59 cell type: differentiated 4 days

Sample_geo_accession	GSM1092486
Sample_status	Public on Mar 05 2013
Sample_submission_date	Mar 04 2013
Sample_last_update_date	Mar 05 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Glioblastoma neurospheres were differentiated in the presence of 10% fetal calf serum and in absence of B-27 supplement for 4days
Sample_growth_protocol_ch1	Tumor cells were resuspended in serum-free DMEM/F12 medium (Invitrogen, Carlsbad, CA) containing human recombinant EGF (20 ng/ml; Sigma, St. Louis, MO), bFGF (20 ng/ml; Sigma), B-27 (20 ml per ml of medium; Invitrogen) and heparin (2 mg/ml), and seeded at a density of 3x106 live cells/60-mm plate
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	The cDNA was prepared from 5 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
Sample_hyb_protocol	Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133A 2.0 chip according to the protocols provided by the manufacturer.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
Sample_data_processing	Data analysis performed using R (2.15.0) and Bioconductor packages. The function rma of package affy (1.36.0) was used for normalization
Sample_platform_id	GPL570
Sample_contact_name	Raquel,,Malumbres
Sample_contact_email	rmalumbres@yahoo.com
Sample_contact_phone	+34 948194700
Sample_contact_laboratory	Molecular Oncology
Sample_contact_department	Oncology
Sample_contact_institute	University of Navarra/ Center for Appl

GSM1092487

GPL570

GBM_neurosphere_4days_4

Glioblastoma multiforme cells, neurospheres, 4 days

tissue: Brain disease status: Glioblastoma multiforme patient: G63 cell type: neurospheres

reanalysis of GSM520526

Sample_geo_accession	GSM1092487
Sample_status	Public on Mar 05 2013
Sample_submission_date	Mar 04 2013
Sample_last_update_date	Mar 05 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Glioblastoma neurospheres were differentiated in the presence of 10% fetal calf serum and in absence of B-27 supplement for 4days
Sample_growth_protocol_ch1	Tumor cells were resuspended in serum-free DMEM/F12 medium (Invitrogen, Carlsbad, CA) containing human recombinant EGF (20 ng/ml; Sigma, St. Louis, MO), bFGF (20 ng/ml; Sigma), B-27 (20 ml per ml of medium; Invitrogen) and heparin (2 mg/ml), and seeded at a density of 3x106 live cells/60-mm plate
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	The cDNA was prepared from 5 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
Sample_hyb_protocol	Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133A 2.0 chip according to the protocols provided by the manufacturer.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
Sample_data_processing	Data analysis performed using R (2.15.0) and Bioconductor packages. The function rma of package affy (1.36.0) was used for normalization
Sample_platform_id	GPL570
Sample_contact_name	Raquel,,Malumbres
Sample_contact_email	rmalumbres@yahoo.com
Sample_contact_phone	+34 948194700
Sample_contact_laboratory	Molecular Oncology
Sample_contact_department	Oncology
Sample_contact_institute	University of Navarra/ Center for Applied Medical Research
Sample_contact_address	Avenida Pio XII 55
Sample_contact_city	Pamplona
Sample_contact_state	Navarra
Sample_contact_zip/postal_code	31008
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1092nnn/GSM1092487/suppl/GSM1092487_G63_NS_12209.CEL.gz
Sample_relation	Reanalysis of: GSM520526
Sample_series_id	GSE44841
Sample_series_id	GSE44843
Sample_data_row_count	54675

GSM1092488

GPL570

GBM_differentiation_4days_4

Glioblastoma multiforme cells, differentiation, 4 days

tissue: Brain disease status: Glioblastoma multiforme patient: G63 cell type: differentiated 4 days

reanalysis of GSM520525

Sample_geo_accession	GSM1092488
Sample_status	Public on Mar 05 2013
Sample_submission_date	Mar 04 2013
Sample_last_update_date	Mar 05 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Glioblastoma neurospheres were differentiated in the presence of 10% fetal calf serum and in absence of B-27 supplement for 4days
Sample_growth_protocol_ch1	Tumor cells were resuspended in serum-free DMEM/F12 medium (Invitrogen, Carlsbad, CA) containing human recombinant EGF (20 ng/ml; Sigma, St. Louis, MO), bFGF (20 ng/ml; Sigma), B-27 (20 ml per ml of medium; Invitrogen) and heparin (2 mg/ml), and seeded at a density of 3x106 live cells/60-mm plate
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	The cDNA was prepared from 5 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
Sample_hyb_protocol	Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133A 2.0 chip according to the protocols provided by the manufacturer.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
Sample_data_processing	Data analysis performed using R (2.15.0) and Bioconductor packages. The function rma of package affy (1.36.0) was used for normalization
Sample_platform_id	GPL570
Sample_contact_name	Raquel,,Malumbres
Sample_contact_email	rmalumbres@yahoo.com
Sample_contact_phone	+34 948194700
Sample_contact_laboratory	Molecular Oncology
Sample_contact_department	Oncology
Sample_contact_institute	University of Navarra/ Center for Applied Medical Research
Sample_contact_address	Avenida Pio XII 55
Sample_contact_city	Pamplona
Sample_contact_state	Navarra
Sample_contact_zip/postal_code	31008
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1092nnn/GSM1092488/suppl/GSM1092488_G63_DF_4d_12209.CEL.gz
Sample_relation	Reanalysis of: GSM520525
Sample_series_id	GSE44841
Sample_series_id	GSE44843
Sample_data_row_count	54675

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