Search results for the GEO ID: GSE44905  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1093796 | GPL570 |
|
LNCaP_vehicle_rep1
|
LNCaP_vehicle
|
cell line: LNCaP
cell type: left supraclavicular lymph node metastatic prostate carcinoma cells
treated with: vehicle (DMSO) for 16hrs
|
A(H0046766.CEL)
|
| Sample_geo_accession | GSM1093796
| | Sample_status | Public on Apr 01 2013
| | Sample_submission_date | Mar 05 2013
| | Sample_last_update_date | Apr 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | LNCaP cells were treated with vehicle (DMSO), DHT (100 nM), enzalutamide (1 or 10 µM) or DHT (100 nM) plus enzalutamide (1 or 10 µM) for 16 hours before RNA extraction.
| | Sample_growth_protocol_ch1 | LNCaP cells were grown in RPMI 1640 supplemented with 5% hormone depleted FBS at 37°C in 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | E.Z.N.A.™ Total RNA kit (Omega Bio-Tek) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using a modified MessageAmp procedure (Life Technologies, Inc.) from 1 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 following Affymetrix standard procedures.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 30007G using GeneChip Operating Software to generate raw and averaged image files (.DAT and .CEL).
| | Sample_data_processing | Affymetrix MAS 5.0 algorithm was used as the scaling (value set to 500) and summarization method. We used a One-way ANOVA (ANalysis Of VAriance) model to test for differentially expressed genes between the groups. For each pair of treatments, a two-sample t-test is also carried out. P-values are corrected to control the false discovery rate (FDR) using the Benjamini & Hochberg method and probes with values less than 0.05 are considered statistically significant [differentially_expressed.txt].
| | Sample_data_processing | The logRatio is the difference in the means of the two groups being compared [logRatio_data.txt]. The means are calculated from the values produced by the MAS5 algorithm, which are in the log2 space
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ivan,Esteban,Alfaro
| | Sample_contact_email | alfobioq@gmail.com
| | Sample_contact_laboratory | Medivation Chile
| | Sample_contact_department | Molecular Innovation and Drug Discovery
| | Sample_contact_institute | Fundacion Ciencia y Vida
| | Sample_contact_address | Zañartu 1482
| | Sample_contact_city | Santiago
| | Sample_contact_zip/postal_code | 7780272
| | Sample_contact_country | Chile
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1093nnn/GSM1093796/suppl/GSM1093796_H0046766.CEL.gz
| | Sample_series_id | GSE44905
| | Sample_data_row_count | 54675
| | |
|
GSM1093797 | GPL570 |
|
LNCaP_vehicle_rep2
|
LNCaP_vehicle
|
cell line: LNCaP
cell type: left supraclavicular lymph node metastatic prostate carcinoma cells
treated with: vehicle (DMSO) for 16hrs
|
A(H0046767.CEL)
|
| Sample_geo_accession | GSM1093797
| | Sample_status | Public on Apr 01 2013
| | Sample_submission_date | Mar 05 2013
| | Sample_last_update_date | Apr 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | LNCaP cells were treated with vehicle (DMSO), DHT (100 nM), enzalutamide (1 or 10 µM) or DHT (100 nM) plus enzalutamide (1 or 10 µM) for 16 hours before RNA extraction.
| | Sample_growth_protocol_ch1 | LNCaP cells were grown in RPMI 1640 supplemented with 5% hormone depleted FBS at 37°C in 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | E.Z.N.A.™ Total RNA kit (Omega Bio-Tek) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using a modified MessageAmp procedure (Life Technologies, Inc.) from 1 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 following Affymetrix standard procedures.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 30007G using GeneChip Operating Software to generate raw and averaged image files (.DAT and .CEL).
| | Sample_data_processing | Affymetrix MAS 5.0 algorithm was used as the scaling (value set to 500) and summarization method. We used a One-way ANOVA (ANalysis Of VAriance) model to test for differentially expressed genes between the groups. For each pair of treatments, a two-sample t-test is also carried out. P-values are corrected to control the false discovery rate (FDR) using the Benjamini & Hochberg method and probes with values less than 0.05 are considered statistically significant [differentially_expressed.txt].
| | Sample_data_processing | The logRatio is the difference in the means of the two groups being compared [logRatio_data.txt]. The means are calculated from the values produced by the MAS5 algorithm, which are in the log2 space
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ivan,Esteban,Alfaro
| | Sample_contact_email | alfobioq@gmail.com
| | Sample_contact_laboratory | Medivation Chile
| | Sample_contact_department | Molecular Innovation and Drug Discovery
| | Sample_contact_institute | Fundacion Ciencia y Vida
| | Sample_contact_address | Zañartu 1482
| | Sample_contact_city | Santiago
| | Sample_contact_zip/postal_code | 7780272
| | Sample_contact_country | Chile
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1093nnn/GSM1093797/suppl/GSM1093797_H0046767.CEL.gz
| | Sample_series_id | GSE44905
| | Sample_data_row_count | 54675
| | |
|
GSM1093798 | GPL570 |
|
LNCaP_vehicle_rep3
|
LNCaP_vehicle
|
cell line: LNCaP
cell type: left supraclavicular lymph node metastatic prostate carcinoma cells
treated with: vehicle (DMSO) for 16hrs
|
A(H0046768.CEL)
|
| Sample_geo_accession | GSM1093798
| | Sample_status | Public on Apr 01 2013
| | Sample_submission_date | Mar 05 2013
| | Sample_last_update_date | Apr 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | LNCaP cells were treated with vehicle (DMSO), DHT (100 nM), enzalutamide (1 or 10 µM) or DHT (100 nM) plus enzalutamide (1 or 10 µM) for 16 hours before RNA extraction.
| | Sample_growth_protocol_ch1 | LNCaP cells were grown in RPMI 1640 supplemented with 5% hormone depleted FBS at 37°C in 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | E.Z.N.A.™ Total RNA kit (Omega Bio-Tek) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using a modified MessageAmp procedure (Life Technologies, Inc.) from 1 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 following Affymetrix standard procedures.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 30007G using GeneChip Operating Software to generate raw and averaged image files (.DAT and .CEL).
| | Sample_data_processing | Affymetrix MAS 5.0 algorithm was used as the scaling (value set to 500) and summarization method. We used a One-way ANOVA (ANalysis Of VAriance) model to test for differentially expressed genes between the groups. For each pair of treatments, a two-sample t-test is also carried out. P-values are corrected to control the false discovery rate (FDR) using the Benjamini & Hochberg method and probes with values less than 0.05 are considered statistically significant [differentially_expressed.txt].
| | Sample_data_processing | The logRatio is the difference in the means of the two groups being compared [logRatio_data.txt]. The means are calculated from the values produced by the MAS5 algorithm, which are in the log2 space
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ivan,Esteban,Alfaro
| | Sample_contact_email | alfobioq@gmail.com
| | Sample_contact_laboratory | Medivation Chile
| | Sample_contact_department | Molecular Innovation and Drug Discovery
| | Sample_contact_institute | Fundacion Ciencia y Vida
| | Sample_contact_address | Zañartu 1482
| | Sample_contact_city | Santiago
| | Sample_contact_zip/postal_code | 7780272
| | Sample_contact_country | Chile
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1093nnn/GSM1093798/suppl/GSM1093798_H0046768.CEL.gz
| | Sample_series_id | GSE44905
| | Sample_data_row_count | 54675
| | |
|
GSM1093799 | GPL570 |
|
LNCaP_DHT 100 nM_rep1
|
LNCaP_DHT 100 nM
|
cell line: LNCaP
cell type: left supraclavicular lymph node metastatic prostate carcinoma cells
treated with: 100 nM DHT for 16hrs
|
B(H0046769.CEL)
|
| Sample_geo_accession | GSM1093799
| | Sample_status | Public on Apr 01 2013
| | Sample_submission_date | Mar 05 2013
| | Sample_last_update_date | Apr 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | LNCaP cells were treated with vehicle (DMSO), DHT (100 nM), enzalutamide (1 or 10 µM) or DHT (100 nM) plus enzalutamide (1 or 10 µM) for 16 hours before RNA extraction.
| | Sample_growth_protocol_ch1 | LNCaP cells were grown in RPMI 1640 supplemented with 5% hormone depleted FBS at 37°C in 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | E.Z.N.A.™ Total RNA kit (Omega Bio-Tek) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using a modified MessageAmp procedure (Life Technologies, Inc.) from 1 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 following Affymetrix standard procedures.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 30007G using GeneChip Operating Software to generate raw and averaged image files (.DAT and .CEL).
| | Sample_data_processing | Affymetrix MAS 5.0 algorithm was used as the scaling (value set to 500) and summarization method. We used a One-way ANOVA (ANalysis Of VAriance) model to test for differentially expressed genes between the groups. For each pair of treatments, a two-sample t-test is also carried out. P-values are corrected to control the false discovery rate (FDR) using the Benjamini & Hochberg method and probes with values less than 0.05 are considered statistically significant [differentially_expressed.txt].
| | Sample_data_processing | The logRatio is the difference in the means of the two groups being compared [logRatio_data.txt]. The means are calculated from the values produced by the MAS5 algorithm, which are in the log2 space
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ivan,Esteban,Alfaro
| | Sample_contact_email | alfobioq@gmail.com
| | Sample_contact_laboratory | Medivation Chile
| | Sample_contact_department | Molecular Innovation and Drug Discovery
| | Sample_contact_institute | Fundacion Ciencia y Vida
| | Sample_contact_address | Zañartu 1482
| | Sample_contact_city | Santiago
| | Sample_contact_zip/postal_code | 7780272
| | Sample_contact_country | Chile
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1093nnn/GSM1093799/suppl/GSM1093799_H0046769.CEL.gz
| | Sample_series_id | GSE44905
| | Sample_data_row_count | 54675
| | |
|
GSM1093800 | GPL570 |
|
LNCaP_DHT 100 nM_rep2
|
LNCaP_DHT 100 nM
|
cell line: LNCaP
cell type: left supraclavicular lymph node metastatic prostate carcinoma cells
treated with: 100 nM DHT for 16hrs
|
B(H0046770.CEL)
|
| Sample_geo_accession | GSM1093800
| | Sample_status | Public on Apr 01 2013
| | Sample_submission_date | Mar 05 2013
| | Sample_last_update_date | Apr 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | LNCaP cells were treated with vehicle (DMSO), DHT (100 nM), enzalutamide (1 or 10 µM) or DHT (100 nM) plus enzalutamide (1 or 10 µM) for 16 hours before RNA extraction.
| | Sample_growth_protocol_ch1 | LNCaP cells were grown in RPMI 1640 supplemented with 5% hormone depleted FBS at 37°C in 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | E.Z.N.A.™ Total RNA kit (Omega Bio-Tek) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using a modified MessageAmp procedure (Life Technologies, Inc.) from 1 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 following Affymetrix standard procedures.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 30007G using GeneChip Operating Software to generate raw and averaged image files (.DAT and .CEL).
| | Sample_data_processing | Affymetrix MAS 5.0 algorithm was used as the scaling (value set to 500) and summarization method. We used a One-way ANOVA (ANalysis Of VAriance) model to test for differentially expressed genes between the groups. For each pair of treatments, a two-sample t-test is also carried out. P-values are corrected to control the false discovery rate (FDR) using the Benjamini & Hochberg method and probes with values less than 0.05 are considered statistically significant [differentially_expressed.txt].
| | Sample_data_processing | The logRatio is the difference in the means of the two groups being compared [logRatio_data.txt]. The means are calculated from the values produced by the MAS5 algorithm, which are in the log2 space
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ivan,Esteban,Alfaro
| | Sample_contact_email | alfobioq@gmail.com
| | Sample_contact_laboratory | Medivation Chile
| | Sample_contact_department | Molecular Innovation and Drug Discovery
| | Sample_contact_institute | Fundacion Ciencia y Vida
| | Sample_contact_address | Zañartu 1482
| | Sample_contact_city | Santiago
| | Sample_contact_zip/postal_code | 7780272
| | Sample_contact_country | Chile
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1093nnn/GSM1093800/suppl/GSM1093800_H0046770.CEL.gz
| | Sample_series_id | GSE44905
| | Sample_data_row_count | 54675
| | |
|
GSM1093801 | GPL570 |
|
LNCaP_DHT 100 nM_rep3
|
LNCaP_DHT 100 nM
|
cell line: LNCaP
cell type: left supraclavicular lymph node metastatic prostate carcinoma cells
treated with: 100 nM DHT for 16hrs
|
B(H0046771.CEL)
|
| Sample_geo_accession | GSM1093801
| | Sample_status | Public on Apr 01 2013
| | Sample_submission_date | Mar 05 2013
| | Sample_last_update_date | Apr 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | LNCaP cells were treated with vehicle (DMSO), DHT (100 nM), enzalutamide (1 or 10 µM) or DHT (100 nM) plus enzalutamide (1 or 10 µM) for 16 hours before RNA extraction.
| | Sample_growth_protocol_ch1 | LNCaP cells were grown in RPMI 1640 supplemented with 5% hormone depleted FBS at 37°C in 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | E.Z.N.A.™ Total RNA kit (Omega Bio-Tek) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using a modified MessageAmp procedure (Life Technologies, Inc.) from 1 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 following Affymetrix standard procedures.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 30007G using GeneChip Operating Software to generate raw and averaged image files (.DAT and .CEL).
| | Sample_data_processing | Affymetrix MAS 5.0 algorithm was used as the scaling (value set to 500) and summarization method. We used a One-way ANOVA (ANalysis Of VAriance) model to test for differentially expressed genes between the groups. For each pair of treatments, a two-sample t-test is also carried out. P-values are corrected to control the false discovery rate (FDR) using the Benjamini & Hochberg method and probes with values less than 0.05 are considered statistically significant [differentially_expressed.txt].
| | Sample_data_processing | The logRatio is the difference in the means of the two groups being compared [logRatio_data.txt]. The means are calculated from the values produced by the MAS5 algorithm, which are in the log2 space
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ivan,Esteban,Alfaro
| | Sample_contact_email | alfobioq@gmail.com
| | Sample_contact_laboratory | Medivation Chile
| | Sample_contact_department | Molecular Innovation and Drug Discovery
| | Sample_contact_institute | Fundacion Ciencia y Vida
| | Sample_contact_address | Zañartu 1482
| | Sample_contact_city | Santiago
| | Sample_contact_zip/postal_code | 7780272
| | Sample_contact_country | Chile
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1093nnn/GSM1093801/suppl/GSM1093801_H0046771.CEL.gz
| | Sample_series_id | GSE44905
| | Sample_data_row_count | 54675
| | |
|
GSM1093802 | GPL570 |
|
LNCaP_enzalutamide 1 x 10E-6 M plus vehicle_rep1
|
LNCaP_enzalutamide 1 x 10E-6 M plus vehicle
|
cell line: LNCaP
cell type: left supraclavicular lymph node metastatic prostate carcinoma cells
treated with: 1 µM enzalutamide plus vehicle for 16hrs
|
C(H0046772.CEL)
|
| Sample_geo_accession | GSM1093802
| | Sample_status | Public on Apr 01 2013
| | Sample_submission_date | Mar 05 2013
| | Sample_last_update_date | Apr 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | LNCaP cells were treated with vehicle (DMSO), DHT (100 nM), enzalutamide (1 or 10 µM) or DHT (100 nM) plus enzalutamide (1 or 10 µM) for 16 hours before RNA extraction.
| | Sample_growth_protocol_ch1 | LNCaP cells were grown in RPMI 1640 supplemented with 5% hormone depleted FBS at 37°C in 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | E.Z.N.A.™ Total RNA kit (Omega Bio-Tek) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using a modified MessageAmp procedure (Life Technologies, Inc.) from 1 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 following Affymetrix standard procedures.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 30007G using GeneChip Operating Software to generate raw and averaged image files (.DAT and .CEL).
| | Sample_data_processing | Affymetrix MAS 5.0 algorithm was used as the scaling (value set to 500) and summarization method. We used a One-way ANOVA (ANalysis Of VAriance) model to test for differentially expressed genes between the groups. For each pair of treatments, a two-sample t-test is also carried out. P-values are corrected to control the false discovery rate (FDR) using the Benjamini & Hochberg method and probes with values less than 0.05 are considered statistically significant [differentially_expressed.txt].
| | Sample_data_processing | The logRatio is the difference in the means of the two groups being compared [logRatio_data.txt]. The means are calculated from the values produced by the MAS5 algorithm, which are in the log2 space
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ivan,Esteban,Alfaro
| | Sample_contact_email | alfobioq@gmail.com
| | Sample_contact_laboratory | Medivation Chile
| | Sample_contact_department | Molecular Innovation and Drug Discovery
| | Sample_contact_institute | Fundacion Ciencia y Vida
| | Sample_contact_address | Zañartu 1482
| | Sample_contact_city | Santiago
| | Sample_contact_zip/postal_code | 7780272
| | Sample_contact_country | Chile
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1093nnn/GSM1093802/suppl/GSM1093802_H0046772.CEL.gz
| | Sample_series_id | GSE44905
| | Sample_data_row_count | 54675
| | |
|
GSM1093803 | GPL570 |
|
LNCaP_enzalutamide 1 x 10E-6 M plus vehicle_rep2
|
LNCaP_enzalutamide 1 x 10E-6 M plus vehicle
|
cell line: LNCaP
cell type: left supraclavicular lymph node metastatic prostate carcinoma cells
treated with: 1 µM enzalutamide plus vehicle for 16hrs
|
C(H0046773.CEL)
|
| Sample_geo_accession | GSM1093803
| | Sample_status | Public on Apr 01 2013
| | Sample_submission_date | Mar 05 2013
| | Sample_last_update_date | Apr 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | LNCaP cells were treated with vehicle (DMSO), DHT (100 nM), enzalutamide (1 or 10 µM) or DHT (100 nM) plus enzalutamide (1 or 10 µM) for 16 hours before RNA extraction.
| | Sample_growth_protocol_ch1 | LNCaP cells were grown in RPMI 1640 supplemented with 5% hormone depleted FBS at 37°C in 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | E.Z.N.A.™ Total RNA kit (Omega Bio-Tek) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using a modified MessageAmp procedure (Life Technologies, Inc.) from 1 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 following Affymetrix standard procedures.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 30007G using GeneChip Operating Software to generate raw and averaged image files (.DAT and .CEL).
| | Sample_data_processing | Affymetrix MAS 5.0 algorithm was used as the scaling (value set to 500) and summarization method. We used a One-way ANOVA (ANalysis Of VAriance) model to test for differentially expressed genes between the groups. For each pair of treatments, a two-sample t-test is also carried out. P-values are corrected to control the false discovery rate (FDR) using the Benjamini & Hochberg method and probes with values less than 0.05 are considered statistically significant [differentially_expressed.txt].
| | Sample_data_processing | The logRatio is the difference in the means of the two groups being compared [logRatio_data.txt]. The means are calculated from the values produced by the MAS5 algorithm, which are in the log2 space
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ivan,Esteban,Alfaro
| | Sample_contact_email | alfobioq@gmail.com
| | Sample_contact_laboratory | Medivation Chile
| | Sample_contact_department | Molecular Innovation and Drug Discovery
| | Sample_contact_institute | Fundacion Ciencia y Vida
| | Sample_contact_address | Zañartu 1482
| | Sample_contact_city | Santiago
| | Sample_contact_zip/postal_code | 7780272
| | Sample_contact_country | Chile
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1093nnn/GSM1093803/suppl/GSM1093803_H0046773.CEL.gz
| | Sample_series_id | GSE44905
| | Sample_data_row_count | 54675
| | |
|
GSM1093804 | GPL570 |
|
LNCaP_enzalutamide 1 x 10E-6 M plus vehicle_rep3
|
LNCaP_enzalutamide 1 x 10E-6 M plus vehicle
|
cell line: LNCaP
cell type: left supraclavicular lymph node metastatic prostate carcinoma cells
treated with: 1 µM enzalutamide plus vehicle for 16hrs
|
C(H0046774.CEL)
|
| Sample_geo_accession | GSM1093804
| | Sample_status | Public on Apr 01 2013
| | Sample_submission_date | Mar 05 2013
| | Sample_last_update_date | Apr 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | LNCaP cells were treated with vehicle (DMSO), DHT (100 nM), enzalutamide (1 or 10 µM) or DHT (100 nM) plus enzalutamide (1 or 10 µM) for 16 hours before RNA extraction.
| | Sample_growth_protocol_ch1 | LNCaP cells were grown in RPMI 1640 supplemented with 5% hormone depleted FBS at 37°C in 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | E.Z.N.A.™ Total RNA kit (Omega Bio-Tek) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using a modified MessageAmp procedure (Life Technologies, Inc.) from 1 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 following Affymetrix standard procedures.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 30007G using GeneChip Operating Software to generate raw and averaged image files (.DAT and .CEL).
| | Sample_data_processing | Affymetrix MAS 5.0 algorithm was used as the scaling (value set to 500) and summarization method. We used a One-way ANOVA (ANalysis Of VAriance) model to test for differentially expressed genes between the groups. For each pair of treatments, a two-sample t-test is also carried out. P-values are corrected to control the false discovery rate (FDR) using the Benjamini & Hochberg method and probes with values less than 0.05 are considered statistically significant [differentially_expressed.txt].
| | Sample_data_processing | The logRatio is the difference in the means of the two groups being compared [logRatio_data.txt]. The means are calculated from the values produced by the MAS5 algorithm, which are in the log2 space
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ivan,Esteban,Alfaro
| | Sample_contact_email | alfobioq@gmail.com
| | Sample_contact_laboratory | Medivation Chile
| | Sample_contact_department | Molecular Innovation and Drug Discovery
| | Sample_contact_institute | Fundacion Ciencia y Vida
| | Sample_contact_address | Zañartu 1482
| | Sample_contact_city | Santiago
| | Sample_contact_zip/postal_code | 7780272
| | Sample_contact_country | Chile
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1093nnn/GSM1093804/suppl/GSM1093804_H0046774.CEL.gz
| | Sample_series_id | GSE44905
| | Sample_data_row_count | 54675
| | |
|
GSM1093805 | GPL570 |
|
LNCaP_enzalutamide 1 x 10E-5 M plus vehicle_rep1
|
LNCaP_enzalutamide 1 x 10E-5 M plus vehicle
|
cell line: LNCaP
cell type: left supraclavicular lymph node metastatic prostate carcinoma cells
treated with: 10 µM enzalutamide plus vehicle for 16hrs
|
D(H0046775.CEL)
|
| Sample_geo_accession | GSM1093805
| | Sample_status | Public on Apr 01 2013
| | Sample_submission_date | Mar 05 2013
| | Sample_last_update_date | Apr 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | LNCaP cells were treated with vehicle (DMSO), DHT (100 nM), enzalutamide (1 or 10 µM) or DHT (100 nM) plus enzalutamide (1 or 10 µM) for 16 hours before RNA extraction.
| | Sample_growth_protocol_ch1 | LNCaP cells were grown in RPMI 1640 supplemented with 5% hormone depleted FBS at 37°C in 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | E.Z.N.A.™ Total RNA kit (Omega Bio-Tek) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using a modified MessageAmp procedure (Life Technologies, Inc.) from 1 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 following Affymetrix standard procedures.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 30007G using GeneChip Operating Software to generate raw and averaged image files (.DAT and .CEL).
| | Sample_data_processing | Affymetrix MAS 5.0 algorithm was used as the scaling (value set to 500) and summarization method. We used a One-way ANOVA (ANalysis Of VAriance) model to test for differentially expressed genes between the groups. For each pair of treatments, a two-sample t-test is also carried out. P-values are corrected to control the false discovery rate (FDR) using the Benjamini & Hochberg method and probes with values less than 0.05 are considered statistically significant [differentially_expressed.txt].
| | Sample_data_processing | The logRatio is the difference in the means of the two groups being compared [logRatio_data.txt]. The means are calculated from the values produced by the MAS5 algorithm, which are in the log2 space
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ivan,Esteban,Alfaro
| | Sample_contact_email | alfobioq@gmail.com
| | Sample_contact_laboratory | Medivation Chile
| | Sample_contact_department | Molecular Innovation and Drug Discovery
| | Sample_contact_institute | Fundacion Ciencia y Vida
| | Sample_contact_address | Zañartu 1482
| | Sample_contact_city | Santiago
| | Sample_contact_zip/postal_code | 7780272
| | Sample_contact_country | Chile
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1093nnn/GSM1093805/suppl/GSM1093805_H0046775.CEL.gz
| | Sample_series_id | GSE44905
| | Sample_data_row_count | 54675
| | |
|
GSM1093806 | GPL570 |
|
LNCaP_enzalutamide 1 x 10E-5 M plus vehicle_rep2
|
LNCaP_enzalutamide 1 x 10E-5 M plus vehicle
|
cell line: LNCaP
cell type: left supraclavicular lymph node metastatic prostate carcinoma cells
treated with: 10 µM enzalutamide plus vehicle for 16hrs
|
D(H0046776.CEL)
|
| Sample_geo_accession | GSM1093806
| | Sample_status | Public on Apr 01 2013
| | Sample_submission_date | Mar 05 2013
| | Sample_last_update_date | Apr 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | LNCaP cells were treated with vehicle (DMSO), DHT (100 nM), enzalutamide (1 or 10 µM) or DHT (100 nM) plus enzalutamide (1 or 10 µM) for 16 hours before RNA extraction.
| | Sample_growth_protocol_ch1 | LNCaP cells were grown in RPMI 1640 supplemented with 5% hormone depleted FBS at 37°C in 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | E.Z.N.A.™ Total RNA kit (Omega Bio-Tek) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using a modified MessageAmp procedure (Life Technologies, Inc.) from 1 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 following Affymetrix standard procedures.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 30007G using GeneChip Operating Software to generate raw and averaged image files (.DAT and .CEL).
| | Sample_data_processing | Affymetrix MAS 5.0 algorithm was used as the scaling (value set to 500) and summarization method. We used a One-way ANOVA (ANalysis Of VAriance) model to test for differentially expressed genes between the groups. For each pair of treatments, a two-sample t-test is also carried out. P-values are corrected to control the false discovery rate (FDR) using the Benjamini & Hochberg method and probes with values less than 0.05 are considered statistically significant [differentially_expressed.txt].
| | Sample_data_processing | The logRatio is the difference in the means of the two groups being compared [logRatio_data.txt]. The means are calculated from the values produced by the MAS5 algorithm, which are in the log2 space
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ivan,Esteban,Alfaro
| | Sample_contact_email | alfobioq@gmail.com
| | Sample_contact_laboratory | Medivation Chile
| | Sample_contact_department | Molecular Innovation and Drug Discovery
| | Sample_contact_institute | Fundacion Ciencia y Vida
| | Sample_contact_address | Zañartu 1482
| | Sample_contact_city | Santiago
| | Sample_contact_zip/postal_code | 7780272
| | Sample_contact_country | Chile
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1093nnn/GSM1093806/suppl/GSM1093806_H0046776.CEL.gz
| | Sample_series_id | GSE44905
| | Sample_data_row_count | 54675
| | |
|
GSM1093807 | GPL570 |
|
LNCaP_enzalutamide 1 x 10E-5 M plus vehicle_rep3
|
LNCaP_enzalutamide 1 x 10E-5 M plus vehicle
|
cell line: LNCaP
cell type: left supraclavicular lymph node metastatic prostate carcinoma cells
treated with: 10 µM enzalutamide plus vehicle for 16hrs
|
D(H0046777.CEL)
|
| Sample_geo_accession | GSM1093807
| | Sample_status | Public on Apr 01 2013
| | Sample_submission_date | Mar 05 2013
| | Sample_last_update_date | Apr 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | LNCaP cells were treated with vehicle (DMSO), DHT (100 nM), enzalutamide (1 or 10 µM) or DHT (100 nM) plus enzalutamide (1 or 10 µM) for 16 hours before RNA extraction.
| | Sample_growth_protocol_ch1 | LNCaP cells were grown in RPMI 1640 supplemented with 5% hormone depleted FBS at 37°C in 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | E.Z.N.A.™ Total RNA kit (Omega Bio-Tek) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using a modified MessageAmp procedure (Life Technologies, Inc.) from 1 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 following Affymetrix standard procedures.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 30007G using GeneChip Operating Software to generate raw and averaged image files (.DAT and .CEL).
| | Sample_data_processing | Affymetrix MAS 5.0 algorithm was used as the scaling (value set to 500) and summarization method. We used a One-way ANOVA (ANalysis Of VAriance) model to test for differentially expressed genes between the groups. For each pair of treatments, a two-sample t-test is also carried out. P-values are corrected to control the false discovery rate (FDR) using the Benjamini & Hochberg method and probes with values less than 0.05 are considered statistically significant [differentially_expressed.txt].
| | Sample_data_processing | The logRatio is the difference in the means of the two groups being compared [logRatio_data.txt]. The means are calculated from the values produced by the MAS5 algorithm, which are in the log2 space
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ivan,Esteban,Alfaro
| | Sample_contact_email | alfobioq@gmail.com
| | Sample_contact_laboratory | Medivation Chile
| | Sample_contact_department | Molecular Innovation and Drug Discovery
| | Sample_contact_institute | Fundacion Ciencia y Vida
| | Sample_contact_address | Zañartu 1482
| | Sample_contact_city | Santiago
| | Sample_contact_zip/postal_code | 7780272
| | Sample_contact_country | Chile
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1093nnn/GSM1093807/suppl/GSM1093807_H0046777.CEL.gz
| | Sample_series_id | GSE44905
| | Sample_data_row_count | 54675
| | |
|
GSM1093808 | GPL570 |
|
LNCaP_enzalutamide 1 x 10E-6 M plus DHT 100 nM_rep1
|
LNCaP_enzalutamide 1 x 10E-6 M plus DHT 100 nM
|
cell line: LNCaP
cell type: left supraclavicular lymph node metastatic prostate carcinoma cells
treated with: 1 µM enzalutamide plus DHT 100 nM for 16hrs
|
E(H0046778.CEL)
|
| Sample_geo_accession | GSM1093808
| | Sample_status | Public on Apr 01 2013
| | Sample_submission_date | Mar 05 2013
| | Sample_last_update_date | Apr 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | LNCaP cells were treated with vehicle (DMSO), DHT (100 nM), enzalutamide (1 or 10 µM) or DHT (100 nM) plus enzalutamide (1 or 10 µM) for 16 hours before RNA extraction.
| | Sample_growth_protocol_ch1 | LNCaP cells were grown in RPMI 1640 supplemented with 5% hormone depleted FBS at 37°C in 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | E.Z.N.A.™ Total RNA kit (Omega Bio-Tek) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using a modified MessageAmp procedure (Life Technologies, Inc.) from 1 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 following Affymetrix standard procedures.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 30007G using GeneChip Operating Software to generate raw and averaged image files (.DAT and .CEL).
| | Sample_data_processing | Affymetrix MAS 5.0 algorithm was used as the scaling (value set to 500) and summarization method. We used a One-way ANOVA (ANalysis Of VAriance) model to test for differentially expressed genes between the groups. For each pair of treatments, a two-sample t-test is also carried out. P-values are corrected to control the false discovery rate (FDR) using the Benjamini & Hochberg method and probes with values less than 0.05 are considered statistically significant [differentially_expressed.txt].
| | Sample_data_processing | The logRatio is the difference in the means of the two groups being compared [logRatio_data.txt]. The means are calculated from the values produced by the MAS5 algorithm, which are in the log2 space
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ivan,Esteban,Alfaro
| | Sample_contact_email | alfobioq@gmail.com
| | Sample_contact_laboratory | Medivation Chile
| | Sample_contact_department | Molecular Innovation and Drug Discovery
| | Sample_contact_institute | Fundacion Ciencia y Vida
| | Sample_contact_address | Zañartu 1482
| | Sample_contact_city | Santiago
| | Sample_contact_zip/postal_code | 7780272
| | Sample_contact_country | Chile
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1093nnn/GSM1093808/suppl/GSM1093808_H0046778.CEL.gz
| | Sample_series_id | GSE44905
| | Sample_data_row_count | 54675
| | |
|
GSM1093809 | GPL570 |
|
LNCaP_enzalutamide 1 x 10E-6 M plus DHT 100 nM_rep2
|
LNCaP_enzalutamide 1 x 10E-6 M plus DHT 100 nM
|
cell line: LNCaP
cell type: left supraclavicular lymph node metastatic prostate carcinoma cells
treated with: 1 µM enzalutamide plus DHT 100 nM for 16hrs
|
E(H0046779.CEL)
|
| Sample_geo_accession | GSM1093809
| | Sample_status | Public on Apr 01 2013
| | Sample_submission_date | Mar 05 2013
| | Sample_last_update_date | Apr 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | LNCaP cells were treated with vehicle (DMSO), DHT (100 nM), enzalutamide (1 or 10 µM) or DHT (100 nM) plus enzalutamide (1 or 10 µM) for 16 hours before RNA extraction.
| | Sample_growth_protocol_ch1 | LNCaP cells were grown in RPMI 1640 supplemented with 5% hormone depleted FBS at 37°C in 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | E.Z.N.A.™ Total RNA kit (Omega Bio-Tek) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using a modified MessageAmp procedure (Life Technologies, Inc.) from 1 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 following Affymetrix standard procedures.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 30007G using GeneChip Operating Software to generate raw and averaged image files (.DAT and .CEL).
| | Sample_data_processing | Affymetrix MAS 5.0 algorithm was used as the scaling (value set to 500) and summarization method. We used a One-way ANOVA (ANalysis Of VAriance) model to test for differentially expressed genes between the groups. For each pair of treatments, a two-sample t-test is also carried out. P-values are corrected to control the false discovery rate (FDR) using the Benjamini & Hochberg method and probes with values less than 0.05 are considered statistically significant [differentially_expressed.txt].
| | Sample_data_processing | The logRatio is the difference in the means of the two groups being compared [logRatio_data.txt]. The means are calculated from the values produced by the MAS5 algorithm, which are in the log2 space
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ivan,Esteban,Alfaro
| | Sample_contact_email | alfobioq@gmail.com
| | Sample_contact_laboratory | Medivation Chile
| | Sample_contact_department | Molecular Innovation and Drug Discovery
| | Sample_contact_institute | Fundacion Ciencia y Vida
| | Sample_contact_address | Zañartu 1482
| | Sample_contact_city | Santiago
| | Sample_contact_zip/postal_code | 7780272
| | Sample_contact_country | Chile
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1093nnn/GSM1093809/suppl/GSM1093809_H0046779.CEL.gz
| | Sample_series_id | GSE44905
| | Sample_data_row_count | 54675
| | |
|
GSM1093810 | GPL570 |
|
LNCaP_enzalutamide 1 x 10E-6 M plus DHT 100 nM_rep3
|
LNCaP_enzalutamide 1 x 10E-6 M plus DHT 100 nM
|
cell line: LNCaP
cell type: left supraclavicular lymph node metastatic prostate carcinoma cells
treated with: 1 µM enzalutamide plus DHT 100 nM for 16hrs
|
E(H0046780.CEL)
|
| Sample_geo_accession | GSM1093810
| | Sample_status | Public on Apr 01 2013
| | Sample_submission_date | Mar 05 2013
| | Sample_last_update_date | Apr 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | LNCaP cells were treated with vehicle (DMSO), DHT (100 nM), enzalutamide (1 or 10 µM) or DHT (100 nM) plus enzalutamide (1 or 10 µM) for 16 hours before RNA extraction.
| | Sample_growth_protocol_ch1 | LNCaP cells were grown in RPMI 1640 supplemented with 5% hormone depleted FBS at 37°C in 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | E.Z.N.A.™ Total RNA kit (Omega Bio-Tek) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using a modified MessageAmp procedure (Life Technologies, Inc.) from 1 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 following Affymetrix standard procedures.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 30007G using GeneChip Operating Software to generate raw and averaged image files (.DAT and .CEL).
| | Sample_data_processing | Affymetrix MAS 5.0 algorithm was used as the scaling (value set to 500) and summarization method. We used a One-way ANOVA (ANalysis Of VAriance) model to test for differentially expressed genes between the groups. For each pair of treatments, a two-sample t-test is also carried out. P-values are corrected to control the false discovery rate (FDR) using the Benjamini & Hochberg method and probes with values less than 0.05 are considered statistically significant [differentially_expressed.txt].
| | Sample_data_processing | The logRatio is the difference in the means of the two groups being compared [logRatio_data.txt]. The means are calculated from the values produced by the MAS5 algorithm, which are in the log2 space
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ivan,Esteban,Alfaro
| | Sample_contact_email | alfobioq@gmail.com
| | Sample_contact_laboratory | Medivation Chile
| | Sample_contact_department | Molecular Innovation and Drug Discovery
| | Sample_contact_institute | Fundacion Ciencia y Vida
| | Sample_contact_address | Zañartu 1482
| | Sample_contact_city | Santiago
| | Sample_contact_zip/postal_code | 7780272
| | Sample_contact_country | Chile
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1093nnn/GSM1093810/suppl/GSM1093810_H0046780.CEL.gz
| | Sample_series_id | GSE44905
| | Sample_data_row_count | 54675
| | |
|
GSM1093811 | GPL570 |
|
LNCaP_enzalutamide 1 x 10E-5 M plus DHT 100 nM_rep1
|
LNCaP_enzalutamide 1 x 10E-5 M plus DHT 100 nM
|
cell line: LNCaP
cell type: left supraclavicular lymph node metastatic prostate carcinoma cells
treated with: 10 µM enzalutamide plus DHT 100 nM for 16hrs
|
F(H0046781.CEL)
|
| Sample_geo_accession | GSM1093811
| | Sample_status | Public on Apr 01 2013
| | Sample_submission_date | Mar 05 2013
| | Sample_last_update_date | Apr 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | LNCaP cells were treated with vehicle (DMSO), DHT (100 nM), enzalutamide (1 or 10 µM) or DHT (100 nM) plus enzalutamide (1 or 10 µM) for 16 hours before RNA extraction.
| | Sample_growth_protocol_ch1 | LNCaP cells were grown in RPMI 1640 supplemented with 5% hormone depleted FBS at 37°C in 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | E.Z.N.A.™ Total RNA kit (Omega Bio-Tek) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using a modified MessageAmp procedure (Life Technologies, Inc.) from 1 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 following Affymetrix standard procedures.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 30007G using GeneChip Operating Software to generate raw and averaged image files (.DAT and .CEL).
| | Sample_data_processing | Affymetrix MAS 5.0 algorithm was used as the scaling (value set to 500) and summarization method. We used a One-way ANOVA (ANalysis Of VAriance) model to test for differentially expressed genes between the groups. For each pair of treatments, a two-sample t-test is also carried out. P-values are corrected to control the false discovery rate (FDR) using the Benjamini & Hochberg method and probes with values less than 0.05 are considered statistically significant [differentially_expressed.txt].
| | Sample_data_processing | The logRatio is the difference in the means of the two groups being compared [logRatio_data.txt]. The means are calculated from the values produced by the MAS5 algorithm, which are in the log2 space
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ivan,Esteban,Alfaro
| | Sample_contact_email | alfobioq@gmail.com
| | Sample_contact_laboratory | Medivation Chile
| | Sample_contact_department | Molecular Innovation and Drug Discovery
| | Sample_contact_institute | Fundacion Ciencia y Vida
| | Sample_contact_address | Zañartu 1482
| | Sample_contact_city | Santiago
| | Sample_contact_zip/postal_code | 7780272
| | Sample_contact_country | Chile
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1093nnn/GSM1093811/suppl/GSM1093811_H0046781.CEL.gz
| | Sample_series_id | GSE44905
| | Sample_data_row_count | 54675
| | |
|
GSM1093812 | GPL570 |
|
LNCaP_enzalutamide 1 x 10E-5 M plus DHT 100 nM_rep2
|
LNCaP_enzalutamide 1 x 10E-5 M plus DHT 100 nM
|
cell line: LNCaP
cell type: left supraclavicular lymph node metastatic prostate carcinoma cells
treated with: 10 µM enzalutamide plus DHT 100 nM for 16hrs
|
F(H0046782.CEL)
|
| Sample_geo_accession | GSM1093812
| | Sample_status | Public on Apr 01 2013
| | Sample_submission_date | Mar 05 2013
| | Sample_last_update_date | Apr 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | LNCaP cells were treated with vehicle (DMSO), DHT (100 nM), enzalutamide (1 or 10 µM) or DHT (100 nM) plus enzalutamide (1 or 10 µM) for 16 hours before RNA extraction.
| | Sample_growth_protocol_ch1 | LNCaP cells were grown in RPMI 1640 supplemented with 5% hormone depleted FBS at 37°C in 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | E.Z.N.A.™ Total RNA kit (Omega Bio-Tek) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using a modified MessageAmp procedure (Life Technologies, Inc.) from 1 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 following Affymetrix standard procedures.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 30007G using GeneChip Operating Software to generate raw and averaged image files (.DAT and .CEL).
| | Sample_data_processing | Affymetrix MAS 5.0 algorithm was used as the scaling (value set to 500) and summarization method. We used a One-way ANOVA (ANalysis Of VAriance) model to test for differentially expressed genes between the groups. For each pair of treatments, a two-sample t-test is also carried out. P-values are corrected to control the false discovery rate (FDR) using the Benjamini & Hochberg method and probes with values less than 0.05 are considered statistically significant [differentially_expressed.txt].
| | Sample_data_processing | The logRatio is the difference in the means of the two groups being compared [logRatio_data.txt]. The means are calculated from the values produced by the MAS5 algorithm, which are in the log2 space
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ivan,Esteban,Alfaro
| | Sample_contact_email | alfobioq@gmail.com
| | Sample_contact_laboratory | Medivation Chile
| | Sample_contact_department | Molecular Innovation and Drug Discovery
| | Sample_contact_institute | Fundacion Ciencia y Vida
| | Sample_contact_address | Zañartu 1482
| | Sample_contact_city | Santiago
| | Sample_contact_zip/postal_code | 7780272
| | Sample_contact_country | Chile
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1093nnn/GSM1093812/suppl/GSM1093812_H0046782.CEL.gz
| | Sample_series_id | GSE44905
| | Sample_data_row_count | 54675
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GSM1093813 | GPL570 |
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LNCaP_enzalutamide 1 x 10E-5 M plus DHT 100 nM_rep3
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LNCaP_enzalutamide 1 x 10E-5 M plus DHT 100 nM
|
cell line: LNCaP
cell type: left supraclavicular lymph node metastatic prostate carcinoma cells
treated with: 10 µM enzalutamide plus DHT 100 nM for 16hrs
|
F(H0046783.CEL)
|
| Sample_geo_accession | GSM1093813
| | Sample_status | Public on Apr 01 2013
| | Sample_submission_date | Mar 05 2013
| | Sample_last_update_date | Apr 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | LNCaP cells were treated with vehicle (DMSO), DHT (100 nM), enzalutamide (1 or 10 µM) or DHT (100 nM) plus enzalutamide (1 or 10 µM) for 16 hours before RNA extraction.
| | Sample_growth_protocol_ch1 | LNCaP cells were grown in RPMI 1640 supplemented with 5% hormone depleted FBS at 37°C in 5% CO2 incubator
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | E.Z.N.A.™ Total RNA kit (Omega Bio-Tek) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using a modified MessageAmp procedure (Life Technologies, Inc.) from 1 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 following Affymetrix standard procedures.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 30007G using GeneChip Operating Software to generate raw and averaged image files (.DAT and .CEL).
| | Sample_data_processing | Affymetrix MAS 5.0 algorithm was used as the scaling (value set to 500) and summarization method. We used a One-way ANOVA (ANalysis Of VAriance) model to test for differentially expressed genes between the groups. For each pair of treatments, a two-sample t-test is also carried out. P-values are corrected to control the false discovery rate (FDR) using the Benjamini & Hochberg method and probes with values less than 0.05 are considered statistically significant [differentially_expressed.txt].
| | Sample_data_processing | The logRatio is the difference in the means of the two groups being compared [logRatio_data.txt]. The means are calculated from the values produced by the MAS5 algorithm, which are in the log2 space
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ivan,Esteban,Alfaro
| | Sample_contact_email | alfobioq@gmail.com
| | Sample_contact_laboratory | Medivation Chile
| | Sample_contact_department | Molecular Innovation and Drug Discovery
| | Sample_contact_institute | Fundacion Ciencia y Vida
| | Sample_contact_address | Zañartu 1482
| | Sample_contact_city | Santiago
| | Sample_contact_zip/postal_code | 7780272
| | Sample_contact_country | Chile
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1093nnn/GSM1093813/suppl/GSM1093813_H0046783.CEL.gz
| | Sample_series_id | GSE44905
| | Sample_data_row_count | 54675
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