Search results for the GEO ID: GSE45005  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1095709 | GPL1261 |
|
ICGN renal cortex_4w_rep1
|
Renal cortex from 4-week-old ICGN mice
|
strain: ICGN
phenotype: nephrotic syndrome
tissue: renal cortex
age: 4 weeks
|
Gene expression data from renal cortex of 4-week-old ICGN mouse (rep 1)
|
| Sample_geo_accession | GSM1095709
| | Sample_status | Public on Jul 31 2013
| | Sample_submission_date | Mar 11 2013
| | Sample_last_update_date | Jul 31 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was harvested from the renal cortex of 4- and 8-week-old ICGN and ICR mice. The samples of renal cortex were separated from the slices fixed in RNAlater (Invitrogen) and homogenized using the Mill Mixer (Qiagen) with zirconium beads. Total RNA was isolated from the kidney homogenate using the RNeasy kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray analysis was conducted by using the GeneChip® Mouse Genome 430 2.0 Array (Affymetrix). The procedure was conducted according to the manufacturer's instructions. The cDNA synthesis and purification was performed with the Superscript Choice System (Invitrogen), the T7-(dT)24-oligonucleotide primer (Affymetrix), and the cDNA Cleanup Module (Affymetrix). The biotinylated cRNA synthesis and purification was performed using the GeneChip Expression 3-Amplification Reagents for IVT Labeling (Affymetrix) and the cRNA Cleanup Module (Affymetrix).
| | Sample_hyb_protocol | Fragmented cRNA (20 µg) was hybridized to a Mouse Genome 430 2.0 Array for 18 hr at 45˚C at 60 rpm, after which the array was washed and stained by streptavidin-phycoerythrin (Fluidics Station 400, Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Kotaro,,Tamura
| | Sample_contact_institute | Astellas Pharma Inc.
| | Sample_contact_address | 2-1-6, Kashima, Yodogawa-ku
| | Sample_contact_city | Osaka
| | Sample_contact_zip/postal_code | 532-8514
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095709/suppl/GSM1095709_ICGN_01_Cortex_Mouse430_2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095709/suppl/GSM1095709_ICGN_01_Cortex_Mouse430_2.CHP.gz
| | Sample_series_id | GSE45005
| | Sample_data_row_count | 45101
| | |
|
GSM1095710 | GPL1261 |
|
ICGN renal cortex_4w_rep2
|
Renal cortex from 4-week-old ICGN mice
|
strain: ICGN
phenotype: nephrotic syndrome
tissue: renal cortex
age: 4 weeks
|
Gene expression data from renal cortex of 4-week-old ICGN mouse (rep 2)
|
| Sample_geo_accession | GSM1095710
| | Sample_status | Public on Jul 31 2013
| | Sample_submission_date | Mar 11 2013
| | Sample_last_update_date | Jul 31 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was harvested from the renal cortex of 4- and 8-week-old ICGN and ICR mice. The samples of renal cortex were separated from the slices fixed in RNAlater (Invitrogen) and homogenized using the Mill Mixer (Qiagen) with zirconium beads. Total RNA was isolated from the kidney homogenate using the RNeasy kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray analysis was conducted by using the GeneChip® Mouse Genome 430 2.0 Array (Affymetrix). The procedure was conducted according to the manufacturer's instructions. The cDNA synthesis and purification was performed with the Superscript Choice System (Invitrogen), the T7-(dT)24-oligonucleotide primer (Affymetrix), and the cDNA Cleanup Module (Affymetrix). The biotinylated cRNA synthesis and purification was performed using the GeneChip Expression 3-Amplification Reagents for IVT Labeling (Affymetrix) and the cRNA Cleanup Module (Affymetrix).
| | Sample_hyb_protocol | Fragmented cRNA (20 µg) was hybridized to a Mouse Genome 430 2.0 Array for 18 hr at 45˚C at 60 rpm, after which the array was washed and stained by streptavidin-phycoerythrin (Fluidics Station 400, Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Kotaro,,Tamura
| | Sample_contact_institute | Astellas Pharma Inc.
| | Sample_contact_address | 2-1-6, Kashima, Yodogawa-ku
| | Sample_contact_city | Osaka
| | Sample_contact_zip/postal_code | 532-8514
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095710/suppl/GSM1095710_ICGN_02_Cortex_Mouse430_2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095710/suppl/GSM1095710_ICGN_02_Cortex_Mouse430_2.CHP.gz
| | Sample_series_id | GSE45005
| | Sample_data_row_count | 45101
| | |
|
GSM1095711 | GPL1261 |
|
ICGN renal cortex_4w_rep3
|
Renal cortex from 4-week-old ICGN mice
|
strain: ICGN
phenotype: nephrotic syndrome
tissue: renal cortex
age: 4 weeks
|
Gene expression data from renal cortex of 4-week-old ICGN mouse (rep 3)
|
| Sample_geo_accession | GSM1095711
| | Sample_status | Public on Jul 31 2013
| | Sample_submission_date | Mar 11 2013
| | Sample_last_update_date | Jul 31 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was harvested from the renal cortex of 4- and 8-week-old ICGN and ICR mice. The samples of renal cortex were separated from the slices fixed in RNAlater (Invitrogen) and homogenized using the Mill Mixer (Qiagen) with zirconium beads. Total RNA was isolated from the kidney homogenate using the RNeasy kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray analysis was conducted by using the GeneChip® Mouse Genome 430 2.0 Array (Affymetrix). The procedure was conducted according to the manufacturer's instructions. The cDNA synthesis and purification was performed with the Superscript Choice System (Invitrogen), the T7-(dT)24-oligonucleotide primer (Affymetrix), and the cDNA Cleanup Module (Affymetrix). The biotinylated cRNA synthesis and purification was performed using the GeneChip Expression 3-Amplification Reagents for IVT Labeling (Affymetrix) and the cRNA Cleanup Module (Affymetrix).
| | Sample_hyb_protocol | Fragmented cRNA (20 µg) was hybridized to a Mouse Genome 430 2.0 Array for 18 hr at 45˚C at 60 rpm, after which the array was washed and stained by streptavidin-phycoerythrin (Fluidics Station 400, Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Kotaro,,Tamura
| | Sample_contact_institute | Astellas Pharma Inc.
| | Sample_contact_address | 2-1-6, Kashima, Yodogawa-ku
| | Sample_contact_city | Osaka
| | Sample_contact_zip/postal_code | 532-8514
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095711/suppl/GSM1095711_ICGN_03_Cortex_Mouse430_2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095711/suppl/GSM1095711_ICGN_03_Cortex_Mouse430_2.CHP.gz
| | Sample_series_id | GSE45005
| | Sample_data_row_count | 45101
| | |
|
GSM1095712 | GPL1261 |
|
ICR renal cortex_4w_rep1
|
Renal cortex from 4-week-old ICR mice
|
strain: ICR
phenotype: normal
tissue: renal cortex
age: 4 weeks
|
Gene expression data from renal cortex of 4-week-old ICR mouse (rep 1)
|
| Sample_geo_accession | GSM1095712
| | Sample_status | Public on Jul 31 2013
| | Sample_submission_date | Mar 11 2013
| | Sample_last_update_date | Jul 31 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was harvested from the renal cortex of 4- and 8-week-old ICGN and ICR mice. The samples of renal cortex were separated from the slices fixed in RNAlater (Invitrogen) and homogenized using the Mill Mixer (Qiagen) with zirconium beads. Total RNA was isolated from the kidney homogenate using the RNeasy kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray analysis was conducted by using the GeneChip® Mouse Genome 430 2.0 Array (Affymetrix). The procedure was conducted according to the manufacturer's instructions. The cDNA synthesis and purification was performed with the Superscript Choice System (Invitrogen), the T7-(dT)24-oligonucleotide primer (Affymetrix), and the cDNA Cleanup Module (Affymetrix). The biotinylated cRNA synthesis and purification was performed using the GeneChip Expression 3-Amplification Reagents for IVT Labeling (Affymetrix) and the cRNA Cleanup Module (Affymetrix).
| | Sample_hyb_protocol | Fragmented cRNA (20 µg) was hybridized to a Mouse Genome 430 2.0 Array for 18 hr at 45˚C at 60 rpm, after which the array was washed and stained by streptavidin-phycoerythrin (Fluidics Station 400, Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Kotaro,,Tamura
| | Sample_contact_institute | Astellas Pharma Inc.
| | Sample_contact_address | 2-1-6, Kashima, Yodogawa-ku
| | Sample_contact_city | Osaka
| | Sample_contact_zip/postal_code | 532-8514
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095712/suppl/GSM1095712_ICR_01_Cortex_Mouse430_2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095712/suppl/GSM1095712_ICR_01_Cortex_Mouse430_2.CHP.gz
| | Sample_series_id | GSE45005
| | Sample_data_row_count | 45101
| | |
|
GSM1095713 | GPL1261 |
|
ICR renal cortex_4w_rep2
|
Renal cortex from 4-week-old ICR mice
|
strain: ICR
phenotype: normal
tissue: renal cortex
age: 4 weeks
|
Gene expression data from renal cortex of 4-week-old ICR mouse (rep 2)
|
| Sample_geo_accession | GSM1095713
| | Sample_status | Public on Jul 31 2013
| | Sample_submission_date | Mar 11 2013
| | Sample_last_update_date | Jul 31 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was harvested from the renal cortex of 4- and 8-week-old ICGN and ICR mice. The samples of renal cortex were separated from the slices fixed in RNAlater (Invitrogen) and homogenized using the Mill Mixer (Qiagen) with zirconium beads. Total RNA was isolated from the kidney homogenate using the RNeasy kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray analysis was conducted by using the GeneChip® Mouse Genome 430 2.0 Array (Affymetrix). The procedure was conducted according to the manufacturer's instructions. The cDNA synthesis and purification was performed with the Superscript Choice System (Invitrogen), the T7-(dT)24-oligonucleotide primer (Affymetrix), and the cDNA Cleanup Module (Affymetrix). The biotinylated cRNA synthesis and purification was performed using the GeneChip Expression 3-Amplification Reagents for IVT Labeling (Affymetrix) and the cRNA Cleanup Module (Affymetrix).
| | Sample_hyb_protocol | Fragmented cRNA (20 µg) was hybridized to a Mouse Genome 430 2.0 Array for 18 hr at 45˚C at 60 rpm, after which the array was washed and stained by streptavidin-phycoerythrin (Fluidics Station 400, Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Kotaro,,Tamura
| | Sample_contact_institute | Astellas Pharma Inc.
| | Sample_contact_address | 2-1-6, Kashima, Yodogawa-ku
| | Sample_contact_city | Osaka
| | Sample_contact_zip/postal_code | 532-8514
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095713/suppl/GSM1095713_ICR_02_Cortex_Mouse430_2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095713/suppl/GSM1095713_ICR_02_Cortex_Mouse430_2.CHP.gz
| | Sample_series_id | GSE45005
| | Sample_data_row_count | 45101
| | |
|
GSM1095714 | GPL1261 |
|
ICR renal cortex_4w_rep3
|
Renal cortex from 4-week-old ICR mice
|
strain: ICR
phenotype: normal
tissue: renal cortex
age: 4 weeks
|
Gene expression data from renal cortex of 4-week-old ICR mouse (rep 3)
|
| Sample_geo_accession | GSM1095714
| | Sample_status | Public on Jul 31 2013
| | Sample_submission_date | Mar 11 2013
| | Sample_last_update_date | Jul 31 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was harvested from the renal cortex of 4- and 8-week-old ICGN and ICR mice. The samples of renal cortex were separated from the slices fixed in RNAlater (Invitrogen) and homogenized using the Mill Mixer (Qiagen) with zirconium beads. Total RNA was isolated from the kidney homogenate using the RNeasy kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray analysis was conducted by using the GeneChip® Mouse Genome 430 2.0 Array (Affymetrix). The procedure was conducted according to the manufacturer's instructions. The cDNA synthesis and purification was performed with the Superscript Choice System (Invitrogen), the T7-(dT)24-oligonucleotide primer (Affymetrix), and the cDNA Cleanup Module (Affymetrix). The biotinylated cRNA synthesis and purification was performed using the GeneChip Expression 3-Amplification Reagents for IVT Labeling (Affymetrix) and the cRNA Cleanup Module (Affymetrix).
| | Sample_hyb_protocol | Fragmented cRNA (20 µg) was hybridized to a Mouse Genome 430 2.0 Array for 18 hr at 45˚C at 60 rpm, after which the array was washed and stained by streptavidin-phycoerythrin (Fluidics Station 400, Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Kotaro,,Tamura
| | Sample_contact_institute | Astellas Pharma Inc.
| | Sample_contact_address | 2-1-6, Kashima, Yodogawa-ku
| | Sample_contact_city | Osaka
| | Sample_contact_zip/postal_code | 532-8514
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095714/suppl/GSM1095714_ICR_03_Cortex_Mouse430_2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095714/suppl/GSM1095714_ICR_03_Cortex_Mouse430_2.CHP.gz
| | Sample_series_id | GSE45005
| | Sample_data_row_count | 45101
| | |
|
GSM1095715 | GPL1261 |
|
ICGN renal cortex_8w_rep1
|
Renal cortex from 8-week-old ICGN mice
|
strain: ICGN
phenotype: nephrotic syndrome
tissue: renal cortex
age: 8 weeks
|
Gene expression data from renal cortex of 8-week-old ICGN mouse (rep 1)
|
| Sample_geo_accession | GSM1095715
| | Sample_status | Public on Jul 31 2013
| | Sample_submission_date | Mar 11 2013
| | Sample_last_update_date | Jul 31 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was harvested from the renal cortex of 4- and 8-week-old ICGN and ICR mice. The samples of renal cortex were separated from the slices fixed in RNAlater (Invitrogen) and homogenized using the Mill Mixer (Qiagen) with zirconium beads. Total RNA was isolated from the kidney homogenate using the RNeasy kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray analysis was conducted by using the GeneChip® Mouse Genome 430 2.0 Array (Affymetrix). The procedure was conducted according to the manufacturer's instructions. The cDNA synthesis and purification was performed with the Superscript Choice System (Invitrogen), the T7-(dT)24-oligonucleotide primer (Affymetrix), and the cDNA Cleanup Module (Affymetrix). The biotinylated cRNA synthesis and purification was performed using the GeneChip Expression 3-Amplification Reagents for IVT Labeling (Affymetrix) and the cRNA Cleanup Module (Affymetrix).
| | Sample_hyb_protocol | Fragmented cRNA (20 µg) was hybridized to a Mouse Genome 430 2.0 Array for 18 hr at 45˚C at 60 rpm, after which the array was washed and stained by streptavidin-phycoerythrin (Fluidics Station 400, Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Kotaro,,Tamura
| | Sample_contact_institute | Astellas Pharma Inc.
| | Sample_contact_address | 2-1-6, Kashima, Yodogawa-ku
| | Sample_contact_city | Osaka
| | Sample_contact_zip/postal_code | 532-8514
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095715/suppl/GSM1095715_8w_ICGN_G1_Cortex_Mouse430_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095715/suppl/GSM1095715_8w_ICGN_G1_Cortex_Mouse430_2_.mas5.CHP.gz
| | Sample_series_id | GSE45005
| | Sample_data_row_count | 45101
| | |
|
GSM1095716 | GPL1261 |
|
ICGN renal cortex_8w_rep2
|
Renal cortex from 8-week-old ICGN mice
|
strain: ICGN
phenotype: nephrotic syndrome
tissue: renal cortex
age: 8 weeks
|
Gene expression data from renal cortex of 8-week-old ICGN mouse (rep 2)
|
| Sample_geo_accession | GSM1095716
| | Sample_status | Public on Jul 31 2013
| | Sample_submission_date | Mar 11 2013
| | Sample_last_update_date | Jul 31 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was harvested from the renal cortex of 4- and 8-week-old ICGN and ICR mice. The samples of renal cortex were separated from the slices fixed in RNAlater (Invitrogen) and homogenized using the Mill Mixer (Qiagen) with zirconium beads. Total RNA was isolated from the kidney homogenate using the RNeasy kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray analysis was conducted by using the GeneChip® Mouse Genome 430 2.0 Array (Affymetrix). The procedure was conducted according to the manufacturer's instructions. The cDNA synthesis and purification was performed with the Superscript Choice System (Invitrogen), the T7-(dT)24-oligonucleotide primer (Affymetrix), and the cDNA Cleanup Module (Affymetrix). The biotinylated cRNA synthesis and purification was performed using the GeneChip Expression 3-Amplification Reagents for IVT Labeling (Affymetrix) and the cRNA Cleanup Module (Affymetrix).
| | Sample_hyb_protocol | Fragmented cRNA (20 µg) was hybridized to a Mouse Genome 430 2.0 Array for 18 hr at 45˚C at 60 rpm, after which the array was washed and stained by streptavidin-phycoerythrin (Fluidics Station 400, Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Kotaro,,Tamura
| | Sample_contact_institute | Astellas Pharma Inc.
| | Sample_contact_address | 2-1-6, Kashima, Yodogawa-ku
| | Sample_contact_city | Osaka
| | Sample_contact_zip/postal_code | 532-8514
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095716/suppl/GSM1095716_8w_ICGN_G2_Cortex_Mouse430_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095716/suppl/GSM1095716_8w_ICGN_G2_Cortex_Mouse430_2_.mas5.CHP.gz
| | Sample_series_id | GSE45005
| | Sample_data_row_count | 45101
| | |
|
GSM1095717 | GPL1261 |
|
ICGN renal cortex_8w_rep3
|
Renal cortex from 8-week-old ICGN mice
|
strain: ICGN
phenotype: nephrotic syndrome
tissue: renal cortex
age: 8 weeks
|
Gene expression data from renal cortex of 8-week-old ICGN mouse (rep 3)
|
| Sample_geo_accession | GSM1095717
| | Sample_status | Public on Jul 31 2013
| | Sample_submission_date | Mar 11 2013
| | Sample_last_update_date | Jul 31 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was harvested from the renal cortex of 4- and 8-week-old ICGN and ICR mice. The samples of renal cortex were separated from the slices fixed in RNAlater (Invitrogen) and homogenized using the Mill Mixer (Qiagen) with zirconium beads. Total RNA was isolated from the kidney homogenate using the RNeasy kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray analysis was conducted by using the GeneChip® Mouse Genome 430 2.0 Array (Affymetrix). The procedure was conducted according to the manufacturer's instructions. The cDNA synthesis and purification was performed with the Superscript Choice System (Invitrogen), the T7-(dT)24-oligonucleotide primer (Affymetrix), and the cDNA Cleanup Module (Affymetrix). The biotinylated cRNA synthesis and purification was performed using the GeneChip Expression 3-Amplification Reagents for IVT Labeling (Affymetrix) and the cRNA Cleanup Module (Affymetrix).
| | Sample_hyb_protocol | Fragmented cRNA (20 µg) was hybridized to a Mouse Genome 430 2.0 Array for 18 hr at 45˚C at 60 rpm, after which the array was washed and stained by streptavidin-phycoerythrin (Fluidics Station 400, Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Kotaro,,Tamura
| | Sample_contact_institute | Astellas Pharma Inc.
| | Sample_contact_address | 2-1-6, Kashima, Yodogawa-ku
| | Sample_contact_city | Osaka
| | Sample_contact_zip/postal_code | 532-8514
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095717/suppl/GSM1095717_8w_ICGN_G3_Cortex_Mouse430_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095717/suppl/GSM1095717_8w_ICGN_G3_Cortex_Mouse430_2_.mas5.CHP.gz
| | Sample_series_id | GSE45005
| | Sample_data_row_count | 45101
| | |
|
GSM1095718 | GPL1261 |
|
ICR renal cortex_8w_rep1
|
Renal cortex from 8-week-old ICR mice
|
strain: ICR
phenotype: normal
tissue: renal cortex
age: 8 weeks
|
Gene expression data from renal cortex of 8-week-old ICR mouse (rep 1)
|
| Sample_geo_accession | GSM1095718
| | Sample_status | Public on Jul 31 2013
| | Sample_submission_date | Mar 11 2013
| | Sample_last_update_date | Jul 31 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was harvested from the renal cortex of 4- and 8-week-old ICGN and ICR mice. The samples of renal cortex were separated from the slices fixed in RNAlater (Invitrogen) and homogenized using the Mill Mixer (Qiagen) with zirconium beads. Total RNA was isolated from the kidney homogenate using the RNeasy kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray analysis was conducted by using the GeneChip® Mouse Genome 430 2.0 Array (Affymetrix). The procedure was conducted according to the manufacturer's instructions. The cDNA synthesis and purification was performed with the Superscript Choice System (Invitrogen), the T7-(dT)24-oligonucleotide primer (Affymetrix), and the cDNA Cleanup Module (Affymetrix). The biotinylated cRNA synthesis and purification was performed using the GeneChip Expression 3-Amplification Reagents for IVT Labeling (Affymetrix) and the cRNA Cleanup Module (Affymetrix).
| | Sample_hyb_protocol | Fragmented cRNA (20 µg) was hybridized to a Mouse Genome 430 2.0 Array for 18 hr at 45˚C at 60 rpm, after which the array was washed and stained by streptavidin-phycoerythrin (Fluidics Station 400, Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Kotaro,,Tamura
| | Sample_contact_institute | Astellas Pharma Inc.
| | Sample_contact_address | 2-1-6, Kashima, Yodogawa-ku
| | Sample_contact_city | Osaka
| | Sample_contact_zip/postal_code | 532-8514
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095718/suppl/GSM1095718_8w_ICR_R5_Cortex_Mouse430_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095718/suppl/GSM1095718_8w_ICR_R5_Cortex_Mouse430_2_.mas5.CHP.gz
| | Sample_series_id | GSE45005
| | Sample_data_row_count | 45101
| | |
|
GSM1095719 | GPL1261 |
|
ICR renal cortex_8w_rep2
|
Renal cortex from 8-week-old ICR mice
|
strain: ICR
phenotype: normal
tissue: renal cortex
age: 8 weeks
|
Gene expression data from renal cortex of 8-week-old ICR mouse (rep 2)
|
| Sample_geo_accession | GSM1095719
| | Sample_status | Public on Jul 31 2013
| | Sample_submission_date | Mar 11 2013
| | Sample_last_update_date | Jul 31 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was harvested from the renal cortex of 4- and 8-week-old ICGN and ICR mice. The samples of renal cortex were separated from the slices fixed in RNAlater (Invitrogen) and homogenized using the Mill Mixer (Qiagen) with zirconium beads. Total RNA was isolated from the kidney homogenate using the RNeasy kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray analysis was conducted by using the GeneChip® Mouse Genome 430 2.0 Array (Affymetrix). The procedure was conducted according to the manufacturer's instructions. The cDNA synthesis and purification was performed with the Superscript Choice System (Invitrogen), the T7-(dT)24-oligonucleotide primer (Affymetrix), and the cDNA Cleanup Module (Affymetrix). The biotinylated cRNA synthesis and purification was performed using the GeneChip Expression 3-Amplification Reagents for IVT Labeling (Affymetrix) and the cRNA Cleanup Module (Affymetrix).
| | Sample_hyb_protocol | Fragmented cRNA (20 µg) was hybridized to a Mouse Genome 430 2.0 Array for 18 hr at 45˚C at 60 rpm, after which the array was washed and stained by streptavidin-phycoerythrin (Fluidics Station 400, Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Kotaro,,Tamura
| | Sample_contact_institute | Astellas Pharma Inc.
| | Sample_contact_address | 2-1-6, Kashima, Yodogawa-ku
| | Sample_contact_city | Osaka
| | Sample_contact_zip/postal_code | 532-8514
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095719/suppl/GSM1095719_8w_ICR_R6_Cortex_Mouse430_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095719/suppl/GSM1095719_8w_ICR_R6_Cortex_Mouse430_2_.mas5.CHP.gz
| | Sample_series_id | GSE45005
| | Sample_data_row_count | 45101
| | |
|
GSM1095720 | GPL1261 |
|
ICR renal cortex_8w_rep3
|
Renal cortex from 8-week-old ICR mice
|
strain: ICR
phenotype: normal
tissue: renal cortex
age: 8 weeks
|
Gene expression data from renal cortex of 8-week-old ICR mouse (rep 3)
|
| Sample_geo_accession | GSM1095720
| | Sample_status | Public on Jul 31 2013
| | Sample_submission_date | Mar 11 2013
| | Sample_last_update_date | Jul 31 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was harvested from the renal cortex of 4- and 8-week-old ICGN and ICR mice. The samples of renal cortex were separated from the slices fixed in RNAlater (Invitrogen) and homogenized using the Mill Mixer (Qiagen) with zirconium beads. Total RNA was isolated from the kidney homogenate using the RNeasy kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray analysis was conducted by using the GeneChip® Mouse Genome 430 2.0 Array (Affymetrix). The procedure was conducted according to the manufacturer's instructions. The cDNA synthesis and purification was performed with the Superscript Choice System (Invitrogen), the T7-(dT)24-oligonucleotide primer (Affymetrix), and the cDNA Cleanup Module (Affymetrix). The biotinylated cRNA synthesis and purification was performed using the GeneChip Expression 3-Amplification Reagents for IVT Labeling (Affymetrix) and the cRNA Cleanup Module (Affymetrix).
| | Sample_hyb_protocol | Fragmented cRNA (20 µg) was hybridized to a Mouse Genome 430 2.0 Array for 18 hr at 45˚C at 60 rpm, after which the array was washed and stained by streptavidin-phycoerythrin (Fluidics Station 400, Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Kotaro,,Tamura
| | Sample_contact_institute | Astellas Pharma Inc.
| | Sample_contact_address | 2-1-6, Kashima, Yodogawa-ku
| | Sample_contact_city | Osaka
| | Sample_contact_zip/postal_code | 532-8514
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095720/suppl/GSM1095720_8w_ICR_R7_Cortex_Mouse430_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095720/suppl/GSM1095720_8w_ICR_R7_Cortex_Mouse430_2_.mas5.CHP.gz
| | Sample_series_id | GSE45005
| | Sample_data_row_count | 45101
| | |
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