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Search results for the GEO ID: GSE45029

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GSM ID

GPL ID

Select for analysis

Title

Source name

Description

Characteristics

GSM1095989

GPL570

MCF12A_control_1

MCF12A breast epithelial cell line, vehicle

cell line: MCF12A cell type: breast epithelial cell line treatment: vehicle

Control-_-Control_1C1

Sample_geo_accession	GSM1095989
Sample_status	Public on Apr 22 2013
Sample_submission_date	Mar 11 2013
Sample_last_update_date	Apr 22 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were treated with doxycycline (dissolved in water) at 1 μg/mL or with vehicle for 4 days. Media, with appropriate treatment, was refreshed after 2 days.
Sample_growth_protocol_ch1	MCF12A breast epithelial cells were grown in 10 cm culture plates in DMEM/F12 supplemented with 5% horse serum, 1% penicillin/streptomycin, 10μg/mL insulin, 0.5 μg/mL hydrocortisone, 20 ng/mL EGF, and 10 μg/mL cholera toxin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using the Qiagen RNeasy kit using the manufacturer's guidelines.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Labeling was performed with the Ambion MessageAmp Premier kit (cat#: 4383452).
Sample_hyb_protocol	Labeled cRNA was mixed with hybridization mix according to the Affymetrix protocol and hybridized in an Affymetrix GeneChip hybridization oven 645 at 45C for 16 hours with rotation at 60 rpm.
Sample_scan_protocol	Scanning was performed on an Affymetrix 7G scanner with target intensity 500.
Sample_data_processing	Data was processed with R/Bioconductor via the RMA method.
Sample_platform_id	GPL570
Sample_contact_name	William,,Sullivan
Sample_contact_email	wsullivan@mednet.ucla.edu
Sample_contact_phone	(310) 206-0163
Sample_contact_laboratory	Heather R. Christofk
Sample_contact_department	Molecular & Medical Pharmacology
Sample_contact_institute	University of California, Los Angeles
Sample_contact_address	650 Charles E Young Drive S, CHS 34-115
Sample_contact_city	Los Angeles
Sample_contact_state	California
Sample_contact_zip/postal_code	90095
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095989/suppl/GSM1095989_Control_1_C1_.CEL.gz
Sample_series_id	GSE45029
Sample_data_row_count	54675

GSM1095990

GPL570

MCF12A_control_2

MCF12A breast epithelial cell line, vehicle

cell line: MCF12A cell type: breast epithelial cell line treatment: vehicle

Control-_-Control_2C2

Sample_geo_accession	GSM1095990
Sample_status	Public on Apr 22 2013
Sample_submission_date	Mar 11 2013
Sample_last_update_date	Apr 22 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were treated with doxycycline (dissolved in water) at 1 μg/mL or with vehicle for 4 days. Media, with appropriate treatment, was refreshed after 2 days.
Sample_growth_protocol_ch1	MCF12A breast epithelial cells were grown in 10 cm culture plates in DMEM/F12 supplemented with 5% horse serum, 1% penicillin/streptomycin, 10μg/mL insulin, 0.5 μg/mL hydrocortisone, 20 ng/mL EGF, and 10 μg/mL cholera toxin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using the Qiagen RNeasy kit using the manufacturer's guidelines.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Labeling was performed with the Ambion MessageAmp Premier kit (cat#: 4383452).
Sample_hyb_protocol	Labeled cRNA was mixed with hybridization mix according to the Affymetrix protocol and hybridized in an Affymetrix GeneChip hybridization oven 645 at 45C for 16 hours with rotation at 60 rpm.
Sample_scan_protocol	Scanning was performed on an Affymetrix 7G scanner with target intensity 500.
Sample_data_processing	Data was processed with R/Bioconductor via the RMA method.
Sample_platform_id	GPL570
Sample_contact_name	William,,Sullivan
Sample_contact_email	wsullivan@mednet.ucla.edu
Sample_contact_phone	(310) 206-0163
Sample_contact_laboratory	Heather R. Christofk
Sample_contact_department	Molecular & Medical Pharmacology
Sample_contact_institute	University of California, Los Angeles
Sample_contact_address	650 Charles E Young Drive S, CHS 34-115
Sample_contact_city	Los Angeles
Sample_contact_state	California
Sample_contact_zip/postal_code	90095
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095990/suppl/GSM1095990_Control_2_C2_.CEL.gz
Sample_series_id	GSE45029
Sample_data_row_count	54675

GSM1095991

GPL570

MCF12A_control_3

MCF12A breast epithelial cell line, vehicle

cell line: MCF12A cell type: breast epithelial cell line treatment: vehicle

Control-_-Control_3C3

Sample_geo_accession	GSM1095991
Sample_status	Public on Apr 22 2013
Sample_submission_date	Mar 11 2013
Sample_last_update_date	Apr 22 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were treated with doxycycline (dissolved in water) at 1 μg/mL or with vehicle for 4 days. Media, with appropriate treatment, was refreshed after 2 days.
Sample_growth_protocol_ch1	MCF12A breast epithelial cells were grown in 10 cm culture plates in DMEM/F12 supplemented with 5% horse serum, 1% penicillin/streptomycin, 10μg/mL insulin, 0.5 μg/mL hydrocortisone, 20 ng/mL EGF, and 10 μg/mL cholera toxin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using the Qiagen RNeasy kit using the manufacturer's guidelines.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Labeling was performed with the Ambion MessageAmp Premier kit (cat#: 4383452).
Sample_hyb_protocol	Labeled cRNA was mixed with hybridization mix according to the Affymetrix protocol and hybridized in an Affymetrix GeneChip hybridization oven 645 at 45C for 16 hours with rotation at 60 rpm.
Sample_scan_protocol	Scanning was performed on an Affymetrix 7G scanner with target intensity 500.
Sample_data_processing	Data was processed with R/Bioconductor via the RMA method.
Sample_platform_id	GPL570
Sample_contact_name	William,,Sullivan
Sample_contact_email	wsullivan@mednet.ucla.edu
Sample_contact_phone	(310) 206-0163
Sample_contact_laboratory	Heather R. Christofk
Sample_contact_department	Molecular & Medical Pharmacology
Sample_contact_institute	University of California, Los Angeles
Sample_contact_address	650 Charles E Young Drive S, CHS 34-115
Sample_contact_city	Los Angeles
Sample_contact_state	California
Sample_contact_zip/postal_code	90095
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095991/suppl/GSM1095991_Control_3_C3_.CEL.gz
Sample_series_id	GSE45029
Sample_data_row_count	54675

GSM1095992

GPL570

MCF12A_Dox_1

MCF12A breast epithelial cell line, Dox

cell line: MCF12A cell type: breast epithelial cell line treatment: doxycycline (1 ug/mL)

High-_-High_1_H1

Sample_geo_accession	GSM1095992
Sample_status	Public on Apr 22 2013
Sample_submission_date	Mar 11 2013
Sample_last_update_date	Apr 22 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were treated with doxycycline (dissolved in water) at 1 μg/mL or with vehicle for 4 days. Media, with appropriate treatment, was refreshed after 2 days.
Sample_growth_protocol_ch1	MCF12A breast epithelial cells were grown in 10 cm culture plates in DMEM/F12 supplemented with 5% horse serum, 1% penicillin/streptomycin, 10μg/mL insulin, 0.5 μg/mL hydrocortisone, 20 ng/mL EGF, and 10 μg/mL cholera toxin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using the Qiagen RNeasy kit using the manufacturer's guidelines.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Labeling was performed with the Ambion MessageAmp Premier kit (cat#: 4383452).
Sample_hyb_protocol	Labeled cRNA was mixed with hybridization mix according to the Affymetrix protocol and hybridized in an Affymetrix GeneChip hybridization oven 645 at 45C for 16 hours with rotation at 60 rpm.
Sample_scan_protocol	Scanning was performed on an Affymetrix 7G scanner with target intensity 500.
Sample_data_processing	Data was processed with R/Bioconductor via the RMA method.
Sample_platform_id	GPL570
Sample_contact_name	William,,Sullivan
Sample_contact_email	wsullivan@mednet.ucla.edu
Sample_contact_phone	(310) 206-0163
Sample_contact_laboratory	Heather R. Christofk
Sample_contact_department	Molecular & Medical Pharmacology
Sample_contact_institute	University of California, Los Angeles
Sample_contact_address	650 Charles E Young Drive S, CHS 34-115
Sample_contact_city	Los Angeles
Sample_contact_state	California
Sample_contact_zip/postal_code	90095
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095992/suppl/GSM1095992_High_1_H1_.CEL.gz
Sample_series_id	GSE45029
Sample_data_row_count	54675

GSM1095993

GPL570

MCF12A_Dox_2

MCF12A breast epithelial cell line, Dox

cell line: MCF12A cell type: breast epithelial cell line treatment: doxycycline (1 ug/mL)

High-_-High_2_H2

Sample_geo_accession	GSM1095993
Sample_status	Public on Apr 22 2013
Sample_submission_date	Mar 11 2013
Sample_last_update_date	Apr 22 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were treated with doxycycline (dissolved in water) at 1 μg/mL or with vehicle for 4 days. Media, with appropriate treatment, was refreshed after 2 days.
Sample_growth_protocol_ch1	MCF12A breast epithelial cells were grown in 10 cm culture plates in DMEM/F12 supplemented with 5% horse serum, 1% penicillin/streptomycin, 10μg/mL insulin, 0.5 μg/mL hydrocortisone, 20 ng/mL EGF, and 10 μg/mL cholera toxin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using the Qiagen RNeasy kit using the manufacturer's guidelines.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Labeling was performed with the Ambion MessageAmp Premier kit (cat#: 4383452).
Sample_hyb_protocol	Labeled cRNA was mixed with hybridization mix according to the Affymetrix protocol and hybridized in an Affymetrix GeneChip hybridization oven 645 at 45C for 16 hours with rotation at 60 rpm.
Sample_scan_protocol	Scanning was performed on an Affymetrix 7G scanner with target intensity 500.
Sample_data_processing	Data was processed with R/Bioconductor via the RMA method.
Sample_platform_id	GPL570
Sample_contact_name	William,,Sullivan
Sample_contact_email	wsullivan@mednet.ucla.edu
Sample_contact_phone	(310) 206-0163
Sample_contact_laboratory	Heather R. Christofk
Sample_contact_department	Molecular & Medical Pharmacology
Sample_contact_institute	University of California, Los Angeles
Sample_contact_address	650 Charles E Young Drive S, CHS 34-115
Sample_contact_city	Los Angeles
Sample_contact_state	California
Sample_contact_zip/postal_code	90095
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095993/suppl/GSM1095993_High_2_H2_.CEL.gz
Sample_series_id	GSE45029
Sample_data_row_count	54675

GSM1095994

GPL570

MCF12A_Dox_3

MCF12A breast epithelial cell line, Dox

cell line: MCF12A cell type: breast epithelial cell line treatment: doxycycline (1 ug/mL)

High-_-High_3_H3

Sample_geo_accession	GSM1095994
Sample_status	Public on Apr 22 2013
Sample_submission_date	Mar 11 2013
Sample_last_update_date	Apr 22 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were treated with doxycycline (dissolved in water) at 1 μg/mL or with vehicle for 4 days. Media, with appropriate treatment, was refreshed after 2 days.
Sample_growth_protocol_ch1	MCF12A breast epithelial cells were grown in 10 cm culture plates in DMEM/F12 supplemented with 5% horse serum, 1% penicillin/streptomycin, 10μg/mL insulin, 0.5 μg/mL hydrocortisone, 20 ng/mL EGF, and 10 μg/mL cholera toxin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using the Qiagen RNeasy kit using the manufacturer's guidelines.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Labeling was performed with the Ambion MessageAmp Premier kit (cat#: 4383452).
Sample_hyb_protocol	Labeled cRNA was mixed with hybridization mix according to the Affymetrix protocol and hybridized in an Affymetrix GeneChip hybridization oven 645 at 45C for 16 hours with rotation at 60 rpm.
Sample_scan_protocol	Scanning was performed on an Affymetrix 7G scanner with target intensity 500.
Sample_data_processing	Data was processed with R/Bioconductor via the RMA method.
Sample_platform_id	GPL570
Sample_contact_name	William,,Sullivan
Sample_contact_email	wsullivan@mednet.ucla.edu
Sample_contact_phone	(310) 206-0163
Sample_contact_laboratory	Heather R. Christofk
Sample_contact_department	Molecular & Medical Pharmacology
Sample_contact_institute	University of California, Los Angeles
Sample_contact_address	650 Charles E Young Drive S, CHS 34-115
Sample_contact_city	Los Angeles
Sample_contact_state	California
Sample_contact_zip/postal_code	90095
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1095nnn/GSM1095994/suppl/GSM1095994_High_3_H3_.CEL.gz
Sample_series_id	GSE45029
Sample_data_row_count	54675

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