Search results for the GEO ID: GSE45044  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1096555 | GPL1261 |
|
VTA_2 months old_rep1
|
C57_VTA_young
|
strain: C57
genotype/variation: wild type
tissue: ventral tegmental area of brain
age: young (2 months old)
|
VTA_2m-1
|
| Sample_geo_accession | GSM1096555
| | Sample_status | Public on May 29 2013
| | Sample_submission_date | Mar 12 2013
| | Sample_last_update_date | May 29 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | three age groups: 2, 10 18 months old C57 wild type mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using RNeasy micro kit (Qiagen) according to the manufacturer's instruction.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 50ng total RNA Two-Cycle Target Labeling Assays (GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
| | Sample_scan_protocol | Microarrays were scanned using GeneChip® Scanner 3000 7G
| | Sample_data_processing | The .DAT image files for each array were converted into .CEL files using Affymetrix AGCC software. These .CEL files were further processed into expression values using Robust Multiarray Average (RMA) method by Irrizarry et al. The following statistical data analysis were performed using the limma (v.3.4.5) package (affylmGUI interface v.1.22.0) of R/Bioconductor project.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lin,,Gao
| | Sample_contact_email | lgao-ibis@us.es
| | Sample_contact_institute | Instituto de Biomedicina de Sevilla
| | Sample_contact_address | Avda Manuel Siurot, Hospital Virgen del Rocío
| | Sample_contact_city | Seville
| | Sample_contact_zip/postal_code | 41013
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1096nnn/GSM1096555/suppl/GSM1096555_VTA2-1.CEL.gz
| | Sample_series_id | GSE45044
| | Sample_series_id | GSE45045
| | Sample_data_row_count | 45101
| | |
|
GSM1096556 | GPL1261 |
|
VTA_2 months old_rep2
|
C57_VTA_young
|
strain: C57
genotype/variation: wild type
tissue: ventral tegmental area of brain
age: young (2 months old)
|
VTA_2m-2
|
| Sample_geo_accession | GSM1096556
| | Sample_status | Public on May 29 2013
| | Sample_submission_date | Mar 12 2013
| | Sample_last_update_date | May 29 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | three age groups: 2, 10 18 months old C57 wild type mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using RNeasy micro kit (Qiagen) according to the manufacturer's instruction.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 50ng total RNA Two-Cycle Target Labeling Assays (GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
| | Sample_scan_protocol | Microarrays were scanned using GeneChip® Scanner 3000 7G
| | Sample_data_processing | The .DAT image files for each array were converted into .CEL files using Affymetrix AGCC software. These .CEL files were further processed into expression values using Robust Multiarray Average (RMA) method by Irrizarry et al. The following statistical data analysis were performed using the limma (v.3.4.5) package (affylmGUI interface v.1.22.0) of R/Bioconductor project.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lin,,Gao
| | Sample_contact_email | lgao-ibis@us.es
| | Sample_contact_institute | Instituto de Biomedicina de Sevilla
| | Sample_contact_address | Avda Manuel Siurot, Hospital Virgen del Rocío
| | Sample_contact_city | Seville
| | Sample_contact_zip/postal_code | 41013
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1096nnn/GSM1096556/suppl/GSM1096556_VTA2-2.CEL.gz
| | Sample_series_id | GSE45044
| | Sample_series_id | GSE45045
| | Sample_data_row_count | 45101
| | |
|
GSM1096557 | GPL1261 |
|
VTA_2 months old_rep3
|
C57_VTA_young
|
strain: C57
genotype/variation: wild type
tissue: ventral tegmental area of brain
age: young (2 months old)
|
VTA_2m-3
|
| Sample_geo_accession | GSM1096557
| | Sample_status | Public on May 29 2013
| | Sample_submission_date | Mar 12 2013
| | Sample_last_update_date | May 29 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | three age groups: 2, 10 18 months old C57 wild type mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using RNeasy micro kit (Qiagen) according to the manufacturer's instruction.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 50ng total RNA Two-Cycle Target Labeling Assays (GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
| | Sample_scan_protocol | Microarrays were scanned using GeneChip® Scanner 3000 7G
| | Sample_data_processing | The .DAT image files for each array were converted into .CEL files using Affymetrix AGCC software. These .CEL files were further processed into expression values using Robust Multiarray Average (RMA) method by Irrizarry et al. The following statistical data analysis were performed using the limma (v.3.4.5) package (affylmGUI interface v.1.22.0) of R/Bioconductor project.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lin,,Gao
| | Sample_contact_email | lgao-ibis@us.es
| | Sample_contact_institute | Instituto de Biomedicina de Sevilla
| | Sample_contact_address | Avda Manuel Siurot, Hospital Virgen del Rocío
| | Sample_contact_city | Seville
| | Sample_contact_zip/postal_code | 41013
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1096nnn/GSM1096557/suppl/GSM1096557_VTA2-3.CEL.gz
| | Sample_series_id | GSE45044
| | Sample_series_id | GSE45045
| | Sample_data_row_count | 45101
| | |
|
GSM1096558 | GPL1261 |
|
VTA_2 months old_rep4
|
C57_VTA_young
|
strain: C57
genotype/variation: wild type
tissue: ventral tegmental area of brain
age: young (2 months old)
|
VTA_2m-4
|
| Sample_geo_accession | GSM1096558
| | Sample_status | Public on May 29 2013
| | Sample_submission_date | Mar 12 2013
| | Sample_last_update_date | May 29 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | three age groups: 2, 10 18 months old C57 wild type mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using RNeasy micro kit (Qiagen) according to the manufacturer's instruction.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 50ng total RNA Two-Cycle Target Labeling Assays (GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
| | Sample_scan_protocol | Microarrays were scanned using GeneChip® Scanner 3000 7G
| | Sample_data_processing | The .DAT image files for each array were converted into .CEL files using Affymetrix AGCC software. These .CEL files were further processed into expression values using Robust Multiarray Average (RMA) method by Irrizarry et al. The following statistical data analysis were performed using the limma (v.3.4.5) package (affylmGUI interface v.1.22.0) of R/Bioconductor project.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lin,,Gao
| | Sample_contact_email | lgao-ibis@us.es
| | Sample_contact_institute | Instituto de Biomedicina de Sevilla
| | Sample_contact_address | Avda Manuel Siurot, Hospital Virgen del Rocío
| | Sample_contact_city | Seville
| | Sample_contact_zip/postal_code | 41013
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1096nnn/GSM1096558/suppl/GSM1096558_VTA2-4.CEL.gz
| | Sample_series_id | GSE45044
| | Sample_series_id | GSE45045
| | Sample_data_row_count | 45101
| | |
|
GSM1096559 | GPL1261 |
|
VTA_10 months old_rep1
|
C57_VTA_middle_aged
|
strain: C57
genotype/variation: wild type
tissue: ventral tegmental area of brain
age: middle aged (10 months old)
|
VTA_10m-1
|
| Sample_geo_accession | GSM1096559
| | Sample_status | Public on May 29 2013
| | Sample_submission_date | Mar 12 2013
| | Sample_last_update_date | May 29 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | three age groups: 2, 10 18 months old C57 wild type mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using RNeasy micro kit (Qiagen) according to the manufacturer's instruction.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 50ng total RNA Two-Cycle Target Labeling Assays (GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
| | Sample_scan_protocol | Microarrays were scanned using GeneChip® Scanner 3000 7G
| | Sample_data_processing | The .DAT image files for each array were converted into .CEL files using Affymetrix AGCC software. These .CEL files were further processed into expression values using Robust Multiarray Average (RMA) method by Irrizarry et al. The following statistical data analysis were performed using the limma (v.3.4.5) package (affylmGUI interface v.1.22.0) of R/Bioconductor project.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lin,,Gao
| | Sample_contact_email | lgao-ibis@us.es
| | Sample_contact_institute | Instituto de Biomedicina de Sevilla
| | Sample_contact_address | Avda Manuel Siurot, Hospital Virgen del Rocío
| | Sample_contact_city | Seville
| | Sample_contact_zip/postal_code | 41013
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1096nnn/GSM1096559/suppl/GSM1096559_VTA10-1.CEL.gz
| | Sample_series_id | GSE45044
| | Sample_series_id | GSE45045
| | Sample_data_row_count | 45101
| | |
|
GSM1096560 | GPL1261 |
|
VTA_10 months old_rep2
|
C57_VTA_middle_aged
|
strain: C57
genotype/variation: wild type
tissue: ventral tegmental area of brain
age: middle aged (10 months old)
|
VTA_10m-2
|
| Sample_geo_accession | GSM1096560
| | Sample_status | Public on May 29 2013
| | Sample_submission_date | Mar 12 2013
| | Sample_last_update_date | May 29 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | three age groups: 2, 10 18 months old C57 wild type mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using RNeasy micro kit (Qiagen) according to the manufacturer's instruction.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 50ng total RNA Two-Cycle Target Labeling Assays (GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
| | Sample_scan_protocol | Microarrays were scanned using GeneChip® Scanner 3000 7G
| | Sample_data_processing | The .DAT image files for each array were converted into .CEL files using Affymetrix AGCC software. These .CEL files were further processed into expression values using Robust Multiarray Average (RMA) method by Irrizarry et al. The following statistical data analysis were performed using the limma (v.3.4.5) package (affylmGUI interface v.1.22.0) of R/Bioconductor project.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lin,,Gao
| | Sample_contact_email | lgao-ibis@us.es
| | Sample_contact_institute | Instituto de Biomedicina de Sevilla
| | Sample_contact_address | Avda Manuel Siurot, Hospital Virgen del Rocío
| | Sample_contact_city | Seville
| | Sample_contact_zip/postal_code | 41013
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1096nnn/GSM1096560/suppl/GSM1096560_VTA10-2.CEL.gz
| | Sample_series_id | GSE45044
| | Sample_series_id | GSE45045
| | Sample_data_row_count | 45101
| | |
|
GSM1096561 | GPL1261 |
|
VTA_10 months old_rep3
|
C57_VTA_middle_aged
|
strain: C57
genotype/variation: wild type
tissue: ventral tegmental area of brain
age: middle aged (10 months old)
|
VTA_10m-3
|
| Sample_geo_accession | GSM1096561
| | Sample_status | Public on May 29 2013
| | Sample_submission_date | Mar 12 2013
| | Sample_last_update_date | May 29 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | three age groups: 2, 10 18 months old C57 wild type mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using RNeasy micro kit (Qiagen) according to the manufacturer's instruction.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 50ng total RNA Two-Cycle Target Labeling Assays (GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
| | Sample_scan_protocol | Microarrays were scanned using GeneChip® Scanner 3000 7G
| | Sample_data_processing | The .DAT image files for each array were converted into .CEL files using Affymetrix AGCC software. These .CEL files were further processed into expression values using Robust Multiarray Average (RMA) method by Irrizarry et al. The following statistical data analysis were performed using the limma (v.3.4.5) package (affylmGUI interface v.1.22.0) of R/Bioconductor project.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lin,,Gao
| | Sample_contact_email | lgao-ibis@us.es
| | Sample_contact_institute | Instituto de Biomedicina de Sevilla
| | Sample_contact_address | Avda Manuel Siurot, Hospital Virgen del Rocío
| | Sample_contact_city | Seville
| | Sample_contact_zip/postal_code | 41013
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1096nnn/GSM1096561/suppl/GSM1096561_VTA10-3.CEL.gz
| | Sample_series_id | GSE45044
| | Sample_series_id | GSE45045
| | Sample_data_row_count | 45101
| | |
|
GSM1096562 | GPL1261 |
|
VTA_10 months old_rep4
|
C57_VTA_middle_aged
|
strain: C57
genotype/variation: wild type
tissue: ventral tegmental area of brain
age: middle aged (10 months old)
|
VTA_10m-4
|
| Sample_geo_accession | GSM1096562
| | Sample_status | Public on May 29 2013
| | Sample_submission_date | Mar 12 2013
| | Sample_last_update_date | May 29 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | three age groups: 2, 10 18 months old C57 wild type mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using RNeasy micro kit (Qiagen) according to the manufacturer's instruction.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 50ng total RNA Two-Cycle Target Labeling Assays (GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
| | Sample_scan_protocol | Microarrays were scanned using GeneChip® Scanner 3000 7G
| | Sample_data_processing | The .DAT image files for each array were converted into .CEL files using Affymetrix AGCC software. These .CEL files were further processed into expression values using Robust Multiarray Average (RMA) method by Irrizarry et al. The following statistical data analysis were performed using the limma (v.3.4.5) package (affylmGUI interface v.1.22.0) of R/Bioconductor project.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lin,,Gao
| | Sample_contact_email | lgao-ibis@us.es
| | Sample_contact_institute | Instituto de Biomedicina de Sevilla
| | Sample_contact_address | Avda Manuel Siurot, Hospital Virgen del Rocío
| | Sample_contact_city | Seville
| | Sample_contact_zip/postal_code | 41013
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1096nnn/GSM1096562/suppl/GSM1096562_VTA10-4.CEL.gz
| | Sample_series_id | GSE45044
| | Sample_series_id | GSE45045
| | Sample_data_row_count | 45101
| | |
|
GSM1096563 | GPL1261 |
|
VTA_18 months old_rep1
|
C57_VTA_late-middle_aged
|
strain: C57
genotype/variation: wild type
tissue: ventral tegmental area of brain
age: late-middle aged (18 months old)
|
VTA_18m-1
|
| Sample_geo_accession | GSM1096563
| | Sample_status | Public on May 29 2013
| | Sample_submission_date | Mar 12 2013
| | Sample_last_update_date | May 29 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | three age groups: 2, 10 18 months old C57 wild type mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using RNeasy micro kit (Qiagen) according to the manufacturer's instruction.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 50ng total RNA Two-Cycle Target Labeling Assays (GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
| | Sample_scan_protocol | Microarrays were scanned using GeneChip® Scanner 3000 7G
| | Sample_data_processing | The .DAT image files for each array were converted into .CEL files using Affymetrix AGCC software. These .CEL files were further processed into expression values using Robust Multiarray Average (RMA) method by Irrizarry et al. The following statistical data analysis were performed using the limma (v.3.4.5) package (affylmGUI interface v.1.22.0) of R/Bioconductor project.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lin,,Gao
| | Sample_contact_email | lgao-ibis@us.es
| | Sample_contact_institute | Instituto de Biomedicina de Sevilla
| | Sample_contact_address | Avda Manuel Siurot, Hospital Virgen del Rocío
| | Sample_contact_city | Seville
| | Sample_contact_zip/postal_code | 41013
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1096nnn/GSM1096563/suppl/GSM1096563_VTA18-1.CEL.gz
| | Sample_series_id | GSE45044
| | Sample_series_id | GSE45045
| | Sample_data_row_count | 45101
| | |
|
GSM1096564 | GPL1261 |
|
VTA_18 months old_rep2
|
C57_VTA_late-middle_aged
|
strain: C57
genotype/variation: wild type
tissue: ventral tegmental area of brain
age: late-middle aged (18 months old)
|
VTA_18m-2
|
| Sample_geo_accession | GSM1096564
| | Sample_status | Public on May 29 2013
| | Sample_submission_date | Mar 12 2013
| | Sample_last_update_date | May 29 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | three age groups: 2, 10 18 months old C57 wild type mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using RNeasy micro kit (Qiagen) according to the manufacturer's instruction.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 50ng total RNA Two-Cycle Target Labeling Assays (GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
| | Sample_scan_protocol | Microarrays were scanned using GeneChip® Scanner 3000 7G
| | Sample_data_processing | The .DAT image files for each array were converted into .CEL files using Affymetrix AGCC software. These .CEL files were further processed into expression values using Robust Multiarray Average (RMA) method by Irrizarry et al. The following statistical data analysis were performed using the limma (v.3.4.5) package (affylmGUI interface v.1.22.0) of R/Bioconductor project.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lin,,Gao
| | Sample_contact_email | lgao-ibis@us.es
| | Sample_contact_institute | Instituto de Biomedicina de Sevilla
| | Sample_contact_address | Avda Manuel Siurot, Hospital Virgen del Rocío
| | Sample_contact_city | Seville
| | Sample_contact_zip/postal_code | 41013
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1096nnn/GSM1096564/suppl/GSM1096564_VTA18-2.CEL.gz
| | Sample_series_id | GSE45044
| | Sample_series_id | GSE45045
| | Sample_data_row_count | 45101
| | |
|
GSM1096565 | GPL1261 |
|
VTA_18 months old_rep3
|
C57_VTA_late-middle_aged
|
strain: C57
genotype/variation: wild type
tissue: ventral tegmental area of brain
age: late-middle aged (18 months old)
|
VTA_18m-3
|
| Sample_geo_accession | GSM1096565
| | Sample_status | Public on May 29 2013
| | Sample_submission_date | Mar 12 2013
| | Sample_last_update_date | May 29 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | three age groups: 2, 10 18 months old C57 wild type mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using RNeasy micro kit (Qiagen) according to the manufacturer's instruction.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 50ng total RNA Two-Cycle Target Labeling Assays (GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
| | Sample_scan_protocol | Microarrays were scanned using GeneChip® Scanner 3000 7G
| | Sample_data_processing | The .DAT image files for each array were converted into .CEL files using Affymetrix AGCC software. These .CEL files were further processed into expression values using Robust Multiarray Average (RMA) method by Irrizarry et al. The following statistical data analysis were performed using the limma (v.3.4.5) package (affylmGUI interface v.1.22.0) of R/Bioconductor project.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lin,,Gao
| | Sample_contact_email | lgao-ibis@us.es
| | Sample_contact_institute | Instituto de Biomedicina de Sevilla
| | Sample_contact_address | Avda Manuel Siurot, Hospital Virgen del Rocío
| | Sample_contact_city | Seville
| | Sample_contact_zip/postal_code | 41013
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1096nnn/GSM1096565/suppl/GSM1096565_VTA18-3.CEL.gz
| | Sample_series_id | GSE45044
| | Sample_series_id | GSE45045
| | Sample_data_row_count | 45101
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GSM1096566 | GPL1261 |
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VTA_18 months old_rep4
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C57_VTA_late-middle_aged
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strain: C57
genotype/variation: wild type
tissue: ventral tegmental area of brain
age: late-middle aged (18 months old)
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VTA_18m-4
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| Sample_geo_accession | GSM1096566
| | Sample_status | Public on May 29 2013
| | Sample_submission_date | Mar 12 2013
| | Sample_last_update_date | May 29 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | three age groups: 2, 10 18 months old C57 wild type mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using RNeasy micro kit (Qiagen) according to the manufacturer's instruction.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 50ng total RNA Two-Cycle Target Labeling Assays (GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
| | Sample_scan_protocol | Microarrays were scanned using GeneChip® Scanner 3000 7G
| | Sample_data_processing | The .DAT image files for each array were converted into .CEL files using Affymetrix AGCC software. These .CEL files were further processed into expression values using Robust Multiarray Average (RMA) method by Irrizarry et al. The following statistical data analysis were performed using the limma (v.3.4.5) package (affylmGUI interface v.1.22.0) of R/Bioconductor project.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lin,,Gao
| | Sample_contact_email | lgao-ibis@us.es
| | Sample_contact_institute | Instituto de Biomedicina de Sevilla
| | Sample_contact_address | Avda Manuel Siurot, Hospital Virgen del Rocío
| | Sample_contact_city | Seville
| | Sample_contact_zip/postal_code | 41013
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1096nnn/GSM1096566/suppl/GSM1096566_VTA18-4.CEL.gz
| | Sample_series_id | GSE45044
| | Sample_series_id | GSE45045
| | Sample_data_row_count | 45101
| | |
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