Search results for the GEO ID: GSE45225 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1099428 | GPL570 |
|
HUVEC_Flow 0_no stent_rep1
|
HUVEC, 0 dyne/cm2, absence of stent
|
cell type: Human Umbelical Vein Endothelial Cell
stress: 0 dyne/cm2
stent: absence
|
Gene expression data from HUVEC at 0 dyne/cm2 in absence of stent
|
Sample_geo_accession | GSM1099428
| Sample_status | Public on Mar 16 2013
| Sample_submission_date | Mar 15 2013
| Sample_last_update_date | Mar 16 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were exposed at different flow in absence or presence of stent procedure in bioreactor for 24 hours. Endothelial cells were washed with PBS and trypsinised. Viable cells were finally collected in RNA later solution (Qiagen) and frozen at -80°.
| Sample_growth_protocol_ch1 | Primary HUVEC were isolated from human cords and grown in ECGM medium (Promocell) in an incubator at 37 ºC. Every 2 days media culture was changed, until the confluence. HUVECs between 2nd and 5th passage were used for bioreactor experiments.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy Micro QIAGEN extraction kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol by Cogentech Service
| Sample_hyb_protocol | Performed by Cogentech Service
| Sample_scan_protocol | Performed by Cogentech Service
| Sample_data_processing | Data Analysis Software: Partek Genomic Suite 6.5; Processing Algorithm: RMA; Probeset Summarization: Median Polish
| Sample_platform_id | GPL570
| Sample_contact_name | Jonica,,Campolo
| Sample_contact_email | jonica.campolo@ospedaleniguarda.it
| Sample_contact_phone | +39026473407
| Sample_contact_department | CardioThoraco and Vascular, Niguarda Hospital
| Sample_contact_institute | CNR Institute of Clinical Phisiology
| Sample_contact_address | Piazza Ospedale Maggiore 3
| Sample_contact_city | Milan
| Sample_contact_state | Milan
| Sample_contact_zip/postal_code | 20162
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1099nnn/GSM1099428/suppl/GSM1099428_F0AS_01.CEL.gz
| Sample_series_id | GSE45225
| Sample_data_row_count | 40508
| |
|
GSM1099429 | GPL570 |
|
HUVEC_Flow 0_no stent_rep2
|
HUVEC, 0 dyne/cm2, absence of stent
|
cell type: Human Umbelical Vein Endothelial Cell
stress: 0 dyne/cm2
stent: absence
|
Gene expression data from HUVEC at 0 dyne/cm2 in absence of stent
|
Sample_geo_accession | GSM1099429
| Sample_status | Public on Mar 16 2013
| Sample_submission_date | Mar 15 2013
| Sample_last_update_date | Mar 16 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were exposed at different flow in absence or presence of stent procedure in bioreactor for 24 hours. Endothelial cells were washed with PBS and trypsinised. Viable cells were finally collected in RNA later solution (Qiagen) and frozen at -80°.
| Sample_growth_protocol_ch1 | Primary HUVEC were isolated from human cords and grown in ECGM medium (Promocell) in an incubator at 37 ºC. Every 2 days media culture was changed, until the confluence. HUVECs between 2nd and 5th passage were used for bioreactor experiments.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy Micro QIAGEN extraction kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol by Cogentech Service
| Sample_hyb_protocol | Performed by Cogentech Service
| Sample_scan_protocol | Performed by Cogentech Service
| Sample_data_processing | Data Analysis Software: Partek Genomic Suite 6.5; Processing Algorithm: RMA; Probeset Summarization: Median Polish
| Sample_platform_id | GPL570
| Sample_contact_name | Jonica,,Campolo
| Sample_contact_email | jonica.campolo@ospedaleniguarda.it
| Sample_contact_phone | +39026473407
| Sample_contact_department | CardioThoraco and Vascular, Niguarda Hospital
| Sample_contact_institute | CNR Institute of Clinical Phisiology
| Sample_contact_address | Piazza Ospedale Maggiore 3
| Sample_contact_city | Milan
| Sample_contact_state | Milan
| Sample_contact_zip/postal_code | 20162
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1099nnn/GSM1099429/suppl/GSM1099429_F0AS_02.CEL.gz
| Sample_series_id | GSE45225
| Sample_data_row_count | 40508
| |
|
GSM1099430 | GPL570 |
|
HUVEC_Flow 1_no stent_rep1
|
HUVEC, 1 dyne/cm2, absence of stent
|
cell type: Human Umbelical Vein Endothelial Cell
stress: 1 dyne/cm2
stent: absence
|
Gene expression data from HUVEC at 1 dyne/cm2 in absence of stent
|
Sample_geo_accession | GSM1099430
| Sample_status | Public on Mar 16 2013
| Sample_submission_date | Mar 15 2013
| Sample_last_update_date | Mar 16 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were exposed at different flow in absence or presence of stent procedure in bioreactor for 24 hours. Endothelial cells were washed with PBS and trypsinised. Viable cells were finally collected in RNA later solution (Qiagen) and frozen at -80°.
| Sample_growth_protocol_ch1 | Primary HUVEC were isolated from human cords and grown in ECGM medium (Promocell) in an incubator at 37 ºC. Every 2 days media culture was changed, until the confluence. HUVECs between 2nd and 5th passage were used for bioreactor experiments.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy Micro QIAGEN extraction kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol by Cogentech Service
| Sample_hyb_protocol | Performed by Cogentech Service
| Sample_scan_protocol | Performed by Cogentech Service
| Sample_data_processing | Data Analysis Software: Partek Genomic Suite 6.5; Processing Algorithm: RMA; Probeset Summarization: Median Polish
| Sample_platform_id | GPL570
| Sample_contact_name | Jonica,,Campolo
| Sample_contact_email | jonica.campolo@ospedaleniguarda.it
| Sample_contact_phone | +39026473407
| Sample_contact_department | CardioThoraco and Vascular, Niguarda Hospital
| Sample_contact_institute | CNR Institute of Clinical Phisiology
| Sample_contact_address | Piazza Ospedale Maggiore 3
| Sample_contact_city | Milan
| Sample_contact_state | Milan
| Sample_contact_zip/postal_code | 20162
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1099nnn/GSM1099430/suppl/GSM1099430_F1AS_03.CEL.gz
| Sample_series_id | GSE45225
| Sample_data_row_count | 40508
| |
|
GSM1099431 | GPL570 |
|
HUVEC_Flow 1_no stent_rep2
|
HUVEC, 1 dyne/cm2, absence of stent
|
cell type: Human Umbelical Vein Endothelial Cell
stress: 1 dyne/cm2
stent: absence
|
Gene expression data from HUVEC at 1 dyne/cm2 in absence of stent
|
Sample_geo_accession | GSM1099431
| Sample_status | Public on Mar 16 2013
| Sample_submission_date | Mar 15 2013
| Sample_last_update_date | Mar 16 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were exposed at different flow in absence or presence of stent procedure in bioreactor for 24 hours. Endothelial cells were washed with PBS and trypsinised. Viable cells were finally collected in RNA later solution (Qiagen) and frozen at -80°.
| Sample_growth_protocol_ch1 | Primary HUVEC were isolated from human cords and grown in ECGM medium (Promocell) in an incubator at 37 ºC. Every 2 days media culture was changed, until the confluence. HUVECs between 2nd and 5th passage were used for bioreactor experiments.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy Micro QIAGEN extraction kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol by Cogentech Service
| Sample_hyb_protocol | Performed by Cogentech Service
| Sample_scan_protocol | Performed by Cogentech Service
| Sample_data_processing | Data Analysis Software: Partek Genomic Suite 6.5; Processing Algorithm: RMA; Probeset Summarization: Median Polish
| Sample_platform_id | GPL570
| Sample_contact_name | Jonica,,Campolo
| Sample_contact_email | jonica.campolo@ospedaleniguarda.it
| Sample_contact_phone | +39026473407
| Sample_contact_department | CardioThoraco and Vascular, Niguarda Hospital
| Sample_contact_institute | CNR Institute of Clinical Phisiology
| Sample_contact_address | Piazza Ospedale Maggiore 3
| Sample_contact_city | Milan
| Sample_contact_state | Milan
| Sample_contact_zip/postal_code | 20162
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1099nnn/GSM1099431/suppl/GSM1099431_F1AS_04.CEL.gz
| Sample_series_id | GSE45225
| Sample_data_row_count | 40508
| |
|
GSM1099432 | GPL570 |
|
HUVEC_Flow 1_with stent_rep1
|
HUVEC, 1 dyne/cm2, presence of stent
|
cell type: Human Umbelical Vein Endothelial Cell
stress: 1 dyne/cm2
stent: presence
|
Gene expression data from HUVEC at 1 dyne/cm2 in presence of stent
|
Sample_geo_accession | GSM1099432
| Sample_status | Public on Mar 16 2013
| Sample_submission_date | Mar 15 2013
| Sample_last_update_date | Mar 16 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were exposed at different flow in absence or presence of stent procedure in bioreactor for 24 hours. Endothelial cells were washed with PBS and trypsinised. Viable cells were finally collected in RNA later solution (Qiagen) and frozen at -80°.
| Sample_growth_protocol_ch1 | Primary HUVEC were isolated from human cords and grown in ECGM medium (Promocell) in an incubator at 37 ºC. Every 2 days media culture was changed, until the confluence. HUVECs between 2nd and 5th passage were used for bioreactor experiments.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy Micro QIAGEN extraction kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol by Cogentech Service
| Sample_hyb_protocol | Performed by Cogentech Service
| Sample_scan_protocol | Performed by Cogentech Service
| Sample_data_processing | Data Analysis Software: Partek Genomic Suite 6.5; Processing Algorithm: RMA; Probeset Summarization: Median Polish
| Sample_platform_id | GPL570
| Sample_contact_name | Jonica,,Campolo
| Sample_contact_email | jonica.campolo@ospedaleniguarda.it
| Sample_contact_phone | +39026473407
| Sample_contact_department | CardioThoraco and Vascular, Niguarda Hospital
| Sample_contact_institute | CNR Institute of Clinical Phisiology
| Sample_contact_address | Piazza Ospedale Maggiore 3
| Sample_contact_city | Milan
| Sample_contact_state | Milan
| Sample_contact_zip/postal_code | 20162
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1099nnn/GSM1099432/suppl/GSM1099432_F1PS_05.CEL.gz
| Sample_series_id | GSE45225
| Sample_data_row_count | 40508
| |
|
GSM1099433 | GPL570 |
|
HUVEC_Flow 1_with stent_rep2
|
HUVEC, 1 dyne/cm2, presence of stent
|
cell type: Human Umbelical Vein Endothelial Cell
stress: 1 dyne/cm2
stent: presence
|
Gene expression data from HUVEC at 1 dyne/cm2 in presence of stent
|
Sample_geo_accession | GSM1099433
| Sample_status | Public on Mar 16 2013
| Sample_submission_date | Mar 15 2013
| Sample_last_update_date | Mar 16 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were exposed at different flow in absence or presence of stent procedure in bioreactor for 24 hours. Endothelial cells were washed with PBS and trypsinised. Viable cells were finally collected in RNA later solution (Qiagen) and frozen at -80°.
| Sample_growth_protocol_ch1 | Primary HUVEC were isolated from human cords and grown in ECGM medium (Promocell) in an incubator at 37 ºC. Every 2 days media culture was changed, until the confluence. HUVECs between 2nd and 5th passage were used for bioreactor experiments.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy Micro QIAGEN extraction kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol by Cogentech Service
| Sample_hyb_protocol | Performed by Cogentech Service
| Sample_scan_protocol | Performed by Cogentech Service
| Sample_data_processing | Data Analysis Software: Partek Genomic Suite 6.5; Processing Algorithm: RMA; Probeset Summarization: Median Polish
| Sample_platform_id | GPL570
| Sample_contact_name | Jonica,,Campolo
| Sample_contact_email | jonica.campolo@ospedaleniguarda.it
| Sample_contact_phone | +39026473407
| Sample_contact_department | CardioThoraco and Vascular, Niguarda Hospital
| Sample_contact_institute | CNR Institute of Clinical Phisiology
| Sample_contact_address | Piazza Ospedale Maggiore 3
| Sample_contact_city | Milan
| Sample_contact_state | Milan
| Sample_contact_zip/postal_code | 20162
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1099nnn/GSM1099433/suppl/GSM1099433_F1PS_06.CEL.gz
| Sample_series_id | GSE45225
| Sample_data_row_count | 40508
| |
|
GSM1099434 | GPL570 |
|
HUVEC_Flow 10_no stent_rep1
|
HUVEC, 10 dyne/cm2, absence of stent
|
cell type: Human Umbelical Vein Endothelial Cell
stress: 10 dyne/cm2
stent: absence
|
Gene expression data from HUVEC at 10 dyne/cm2 in absence of stent
|
Sample_geo_accession | GSM1099434
| Sample_status | Public on Mar 16 2013
| Sample_submission_date | Mar 15 2013
| Sample_last_update_date | Mar 16 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were exposed at different flow in absence or presence of stent procedure in bioreactor for 24 hours. Endothelial cells were washed with PBS and trypsinised. Viable cells were finally collected in RNA later solution (Qiagen) and frozen at -80°.
| Sample_growth_protocol_ch1 | Primary HUVEC were isolated from human cords and grown in ECGM medium (Promocell) in an incubator at 37 ºC. Every 2 days media culture was changed, until the confluence. HUVECs between 2nd and 5th passage were used for bioreactor experiments.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy Micro QIAGEN extraction kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol by Cogentech Service
| Sample_hyb_protocol | Performed by Cogentech Service
| Sample_scan_protocol | Performed by Cogentech Service
| Sample_data_processing | Data Analysis Software: Partek Genomic Suite 6.5; Processing Algorithm: RMA; Probeset Summarization: Median Polish
| Sample_platform_id | GPL570
| Sample_contact_name | Jonica,,Campolo
| Sample_contact_email | jonica.campolo@ospedaleniguarda.it
| Sample_contact_phone | +39026473407
| Sample_contact_department | CardioThoraco and Vascular, Niguarda Hospital
| Sample_contact_institute | CNR Institute of Clinical Phisiology
| Sample_contact_address | Piazza Ospedale Maggiore 3
| Sample_contact_city | Milan
| Sample_contact_state | Milan
| Sample_contact_zip/postal_code | 20162
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1099nnn/GSM1099434/suppl/GSM1099434_F10AS_07.CEL.gz
| Sample_series_id | GSE45225
| Sample_data_row_count | 40508
| |
|
GSM1099435 | GPL570 |
|
HUVEC_Flow 10_no stent_rep2
|
HUVEC, 10 dyne/cm2, absence of stent
|
cell type: Human Umbelical Vein Endothelial Cell
stress: 10 dyne/cm2
stent: absence
|
Gene expression data from HUVEC at 10 dyne/cm2 in absence of stent
|
Sample_geo_accession | GSM1099435
| Sample_status | Public on Mar 16 2013
| Sample_submission_date | Mar 15 2013
| Sample_last_update_date | Mar 16 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were exposed at different flow in absence or presence of stent procedure in bioreactor for 24 hours. Endothelial cells were washed with PBS and trypsinised. Viable cells were finally collected in RNA later solution (Qiagen) and frozen at -80°.
| Sample_growth_protocol_ch1 | Primary HUVEC were isolated from human cords and grown in ECGM medium (Promocell) in an incubator at 37 ºC. Every 2 days media culture was changed, until the confluence. HUVECs between 2nd and 5th passage were used for bioreactor experiments.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy Micro QIAGEN extraction kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol by Cogentech Service
| Sample_hyb_protocol | Performed by Cogentech Service
| Sample_scan_protocol | Performed by Cogentech Service
| Sample_data_processing | Data Analysis Software: Partek Genomic Suite 6.5; Processing Algorithm: RMA; Probeset Summarization: Median Polish
| Sample_platform_id | GPL570
| Sample_contact_name | Jonica,,Campolo
| Sample_contact_email | jonica.campolo@ospedaleniguarda.it
| Sample_contact_phone | +39026473407
| Sample_contact_department | CardioThoraco and Vascular, Niguarda Hospital
| Sample_contact_institute | CNR Institute of Clinical Phisiology
| Sample_contact_address | Piazza Ospedale Maggiore 3
| Sample_contact_city | Milan
| Sample_contact_state | Milan
| Sample_contact_zip/postal_code | 20162
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1099nnn/GSM1099435/suppl/GSM1099435_F10AS_08.CEL.gz
| Sample_series_id | GSE45225
| Sample_data_row_count | 40508
| |
|
GSM1099436 | GPL570 |
|
HUVEC_Flow 10_with stent_rep1
|
HUVEC, 10 dyne/cm2, presence of stent
|
cell type: Human Umbelical Vein Endothelial Cell
stress: 10 dyne/cm2
stent: presence
|
Gene expression data from HUVEC at 10 dyne/cm2 in presence of stent
|
Sample_geo_accession | GSM1099436
| Sample_status | Public on Mar 16 2013
| Sample_submission_date | Mar 15 2013
| Sample_last_update_date | Mar 16 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were exposed at different flow in absence or presence of stent procedure in bioreactor for 24 hours. Endothelial cells were washed with PBS and trypsinised. Viable cells were finally collected in RNA later solution (Qiagen) and frozen at -80°.
| Sample_growth_protocol_ch1 | Primary HUVEC were isolated from human cords and grown in ECGM medium (Promocell) in an incubator at 37 ºC. Every 2 days media culture was changed, until the confluence. HUVECs between 2nd and 5th passage were used for bioreactor experiments.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy Micro QIAGEN extraction kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol by Cogentech Service
| Sample_hyb_protocol | Performed by Cogentech Service
| Sample_scan_protocol | Performed by Cogentech Service
| Sample_data_processing | Data Analysis Software: Partek Genomic Suite 6.5; Processing Algorithm: RMA; Probeset Summarization: Median Polish
| Sample_platform_id | GPL570
| Sample_contact_name | Jonica,,Campolo
| Sample_contact_email | jonica.campolo@ospedaleniguarda.it
| Sample_contact_phone | +39026473407
| Sample_contact_department | CardioThoraco and Vascular, Niguarda Hospital
| Sample_contact_institute | CNR Institute of Clinical Phisiology
| Sample_contact_address | Piazza Ospedale Maggiore 3
| Sample_contact_city | Milan
| Sample_contact_state | Milan
| Sample_contact_zip/postal_code | 20162
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1099nnn/GSM1099436/suppl/GSM1099436_F10PS_09.CEL.gz
| Sample_series_id | GSE45225
| Sample_data_row_count | 40508
| |
|
GSM1099437 | GPL570 |
|
HUVEC_Flow 10_with stent_rep2
|
HUVEC, 10 dyne/cm2, presence of stent
|
cell type: Human Umbelical Vein Endothelial Cell
stress: 10 dyne/cm2
stent: presence
|
Gene expression data from HUVEC at 10 dyne/cm2 in presence of stent
|
Sample_geo_accession | GSM1099437
| Sample_status | Public on Mar 16 2013
| Sample_submission_date | Mar 15 2013
| Sample_last_update_date | Mar 16 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were exposed at different flow in absence or presence of stent procedure in bioreactor for 24 hours. Endothelial cells were washed with PBS and trypsinised. Viable cells were finally collected in RNA later solution (Qiagen) and frozen at -80°.
| Sample_growth_protocol_ch1 | Primary HUVEC were isolated from human cords and grown in ECGM medium (Promocell) in an incubator at 37 ºC. Every 2 days media culture was changed, until the confluence. HUVECs between 2nd and 5th passage were used for bioreactor experiments.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy Micro QIAGEN extraction kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol by Cogentech Service
| Sample_hyb_protocol | Performed by Cogentech Service
| Sample_scan_protocol | Performed by Cogentech Service
| Sample_data_processing | Data Analysis Software: Partek Genomic Suite 6.5; Processing Algorithm: RMA; Probeset Summarization: Median Polish
| Sample_platform_id | GPL570
| Sample_contact_name | Jonica,,Campolo
| Sample_contact_email | jonica.campolo@ospedaleniguarda.it
| Sample_contact_phone | +39026473407
| Sample_contact_department | CardioThoraco and Vascular, Niguarda Hospital
| Sample_contact_institute | CNR Institute of Clinical Phisiology
| Sample_contact_address | Piazza Ospedale Maggiore 3
| Sample_contact_city | Milan
| Sample_contact_state | Milan
| Sample_contact_zip/postal_code | 20162
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1099nnn/GSM1099437/suppl/GSM1099437_F10PS_10.CEL.gz
| Sample_series_id | GSE45225
| Sample_data_row_count | 40508
| |
|
GSM1099438 | GPL570 |
|
HUVEC_Flow 5_no stent_rep1
|
HUVEC, 5 dyne/cm2, absence of stent
|
cell type: Human Umbelical Vein Endothelial Cell
stress: 5 dyne/cm2
stent: absence
|
Gene expression data from HUVEC at 5 dyne/cm2 in absence of stent
|
Sample_geo_accession | GSM1099438
| Sample_status | Public on Mar 16 2013
| Sample_submission_date | Mar 15 2013
| Sample_last_update_date | Mar 16 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were exposed at different flow in absence or presence of stent procedure in bioreactor for 24 hours. Endothelial cells were washed with PBS and trypsinised. Viable cells were finally collected in RNA later solution (Qiagen) and frozen at -80°.
| Sample_growth_protocol_ch1 | Primary HUVEC were isolated from human cords and grown in ECGM medium (Promocell) in an incubator at 37 ºC. Every 2 days media culture was changed, until the confluence. HUVECs between 2nd and 5th passage were used for bioreactor experiments.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy Micro QIAGEN extraction kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol by Cogentech Service
| Sample_hyb_protocol | Performed by Cogentech Service
| Sample_scan_protocol | Performed by Cogentech Service
| Sample_data_processing | Data Analysis Software: Partek Genomic Suite 6.5; Processing Algorithm: RMA; Probeset Summarization: Median Polish
| Sample_platform_id | GPL570
| Sample_contact_name | Jonica,,Campolo
| Sample_contact_email | jonica.campolo@ospedaleniguarda.it
| Sample_contact_phone | +39026473407
| Sample_contact_department | CardioThoraco and Vascular, Niguarda Hospital
| Sample_contact_institute | CNR Institute of Clinical Phisiology
| Sample_contact_address | Piazza Ospedale Maggiore 3
| Sample_contact_city | Milan
| Sample_contact_state | Milan
| Sample_contact_zip/postal_code | 20162
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1099nnn/GSM1099438/suppl/GSM1099438_F5AS_11.CEL.gz
| Sample_series_id | GSE45225
| Sample_data_row_count | 40508
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GSM1099439 | GPL570 |
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HUVEC_Flow 5_no stent_rep2
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HUVEC, 5 dyne/cm2, absence of stent
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cell type: Human Umbelical Vein Endothelial Cell
stress: 5 dyne/cm2
stent: absence
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Gene expression data from HUVEC at 5 dyne/cm2 in absence of stent
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Sample_geo_accession | GSM1099439
| Sample_status | Public on Mar 16 2013
| Sample_submission_date | Mar 15 2013
| Sample_last_update_date | Mar 16 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were exposed at different flow in absence or presence of stent procedure in bioreactor for 24 hours. Endothelial cells were washed with PBS and trypsinised. Viable cells were finally collected in RNA later solution (Qiagen) and frozen at -80°.
| Sample_growth_protocol_ch1 | Primary HUVEC were isolated from human cords and grown in ECGM medium (Promocell) in an incubator at 37 ºC. Every 2 days media culture was changed, until the confluence. HUVECs between 2nd and 5th passage were used for bioreactor experiments.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy Micro QIAGEN extraction kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol by Cogentech Service
| Sample_hyb_protocol | Performed by Cogentech Service
| Sample_scan_protocol | Performed by Cogentech Service
| Sample_data_processing | Data Analysis Software: Partek Genomic Suite 6.5; Processing Algorithm: RMA; Probeset Summarization: Median Polish
| Sample_platform_id | GPL570
| Sample_contact_name | Jonica,,Campolo
| Sample_contact_email | jonica.campolo@ospedaleniguarda.it
| Sample_contact_phone | +39026473407
| Sample_contact_department | CardioThoraco and Vascular, Niguarda Hospital
| Sample_contact_institute | CNR Institute of Clinical Phisiology
| Sample_contact_address | Piazza Ospedale Maggiore 3
| Sample_contact_city | Milan
| Sample_contact_state | Milan
| Sample_contact_zip/postal_code | 20162
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1099nnn/GSM1099439/suppl/GSM1099439_F5AS_12.CEL.gz
| Sample_series_id | GSE45225
| Sample_data_row_count | 40508
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