Sample_geo_accession | GSM1103492
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Sample_status | Public on May 22 2013
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Sample_submission_date | Mar 21 2013
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Sample_last_update_date | May 22 2013
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Sample_type | RNA
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Sample_channel_count | 1
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Sample_organism_ch1 | Homo sapiens
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Sample_taxid_ch1 | 9606
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Sample_treatment_protocol_ch1 | MKN28 cell cultures expressing Sox2 were generated by infecting MKN28 cells with lenti-Sox2 (GOSL24628, Shanghai GeneChem Co., Ltd) using puromycin (2μg/mL) selection for 3-5 days. The lentivirus Sox2 provides the puromycin resistance.
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Sample_growth_protocol_ch1 | Human gastric cancer cell lines MKN28 were cultured at 37°C in an atmosphere of 5% CO2 in RPMI1640 (HyClone) supplemented with 10% fetal bovine serum, 100 unit penicillin, and 100 μg/mL streptomycin (Gibco BRL, Life Technologies, New York, USA).
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Sample_molecule_ch1 | total RNA
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Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
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Sample_label_ch1 | biotin
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Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
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Sample_hyb_protocol | Hybridization with Affymetrix Hybridization Oven 640 according to the standard Affymetrix protocol. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
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Sample_data_processing | Robust Multichip Analysis(RMA)
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Sample_platform_id | GPL570
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Sample_contact_name | Wang,,Simeng
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Sample_contact_laboratory | State Key Laboratory of Cancer Biology
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Sample_contact_department | Xijing Hospital of Digestive Diseases
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Sample_contact_institute | Fourth Military Medical University
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Sample_contact_address | Changle Road
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Sample_contact_city | Xi'an
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Sample_contact_zip/postal_code | 710032
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Sample_contact_country | China
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Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1103nnn/GSM1103492/suppl/GSM1103492_100910-10481A_S_7.29_HG-U133_Plus_2_.CEL.gz
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Sample_series_id | GSE45391
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Sample_data_row_count | 54675
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