Result

Search results for the GEO ID: GSE45516

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM1106065

GPL570

ctrl1

dermal fibroblast

disease: normal cell type: Fibroblast cells age: 28 Sex: male

Gene expression profile comparison from fibroblasts of Huntington individuals and normal ones

Sample_geo_accession	GSM1106065
Sample_status	Public on Mar 28 2013
Sample_submission_date	Mar 26 2013
Sample_last_update_date	Mar 28 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	none
Sample_growth_protocol_ch1	Fibroblast cells were cultured in Eagle's minimum essential (MEM) supplemented with 10% FBS, 100 mg/mL streptomycin, 100 U/mL penicillin and incubated at 37 C in a humidified chamber with 5% CO2. Cells were harvested once they reached confluence by treating with trypsin (0.05% trypsin with 0.25% EDTA).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA followed by Qiagen cleanup were performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 plus Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	The GeneChip was scanned and data extracted using GeneChip scanner 3000 7G (Affimetrix, SantanClara, CA).
Sample_data_processing	The data were analyzed with Expression Console™ Software using Affymetrix default analysis settings and Robust Multichip Analysis (RMA). Subsequently analyses for differentially expressed genes cathegorization were performed with OnechannelGUI in R environment
Sample_platform_id	GPL570
Sample_contact_name	andrea,,bozzato
Sample_contact_email	bzzandrew@yahoo.it
Sample_contact_institute	university
Sample_contact_address	via bassi
Sample_contact_city	padova
Sample_contact_zip/postal_code	35123
Sample_contact_country	Italy
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1106nnn/GSM1106065/suppl/GSM1106065_BS07_2.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1106nnn/GSM1106065/suppl/GSM1106065_BS07_2.rma.chp.gz
Sample_series_id	GSE45516
Sample_data_row_count	54675

GSM1106066

GPL570

ctrl2

dermal fibroblast

disease: normal cell type: Fibroblast cells age: 48 Sex: male

Gene expression profile comparison from fibroblasts of Huntington individuals and normal ones

Sample_geo_accession	GSM1106066
Sample_status	Public on Mar 28 2013
Sample_submission_date	Mar 26 2013
Sample_last_update_date	Mar 28 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	none
Sample_growth_protocol_ch1	Fibroblast cells were cultured in Eagle's minimum essential (MEM) supplemented with 10% FBS, 100 mg/mL streptomycin, 100 U/mL penicillin and incubated at 37 C in a humidified chamber with 5% CO2. Cells were harvested once they reached confluence by treating with trypsin (0.05% trypsin with 0.25% EDTA).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA followed by Qiagen cleanup were performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 plus Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	The GeneChip was scanned and data extracted using GeneChip scanner 3000 7G (Affimetrix, SantanClara, CA).
Sample_data_processing	The data were analyzed with Expression Console™ Software using Affymetrix default analysis settings and Robust Multichip Analysis (RMA). Subsequently analyses for differentially expressed genes cathegorization were performed with OnechannelGUI in R environment
Sample_platform_id	GPL570
Sample_contact_name	andrea,,bozzato
Sample_contact_email	bzzandrew@yahoo.it
Sample_contact_institute	university
Sample_contact_address	via bassi
Sample_contact_city	padova
Sample_contact_zip/postal_code	35123
Sample_contact_country	Italy
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1106nnn/GSM1106066/suppl/GSM1106066_BS10_2.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1106nnn/GSM1106066/suppl/GSM1106066_BS10_2.rma.chp.gz
Sample_series_id	GSE45516
Sample_data_row_count	54675

GSM1106067

GPL570

ctrl3

dermal fibroblast

disease: normal cell type: Fibroblast cells age: 57 Sex: male

Gene expression profile comparison from fibroblasts of Huntington individuals and normal ones

Sample_geo_accession	GSM1106067
Sample_status	Public on Mar 28 2013
Sample_submission_date	Mar 26 2013
Sample_last_update_date	Mar 28 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	none
Sample_growth_protocol_ch1	Fibroblast cells were cultured in Eagle's minimum essential (MEM) supplemented with 10% FBS, 100 mg/mL streptomycin, 100 U/mL penicillin and incubated at 37 C in a humidified chamber with 5% CO2. Cells were harvested once they reached confluence by treating with trypsin (0.05% trypsin with 0.25% EDTA).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA followed by Qiagen cleanup were performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 plus Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	The GeneChip was scanned and data extracted using GeneChip scanner 3000 7G (Affimetrix, SantanClara, CA).
Sample_data_processing	The data were analyzed with Expression Console™ Software using Affymetrix default analysis settings and Robust Multichip Analysis (RMA). Subsequently analyses for differentially expressed genes cathegorization were performed with OnechannelGUI in R environment
Sample_platform_id	GPL570
Sample_contact_name	andrea,,bozzato
Sample_contact_email	bzzandrew@yahoo.it
Sample_contact_institute	university
Sample_contact_address	via bassi
Sample_contact_city	padova
Sample_contact_zip/postal_code	35123
Sample_contact_country	Italy
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1106nnn/GSM1106067/suppl/GSM1106067_AB3_2.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1106nnn/GSM1106067/suppl/GSM1106067_AB3_2.rma.chp.gz
Sample_series_id	GSE45516
Sample_data_row_count	54675

GSM1106068

GPL570

hd1

dermal fibroblast

disease: Huntington cell type: Fibroblast cells age: 38 Sex: male

Gene expression profile comparison from fibroblasts of Huntington individuals and normal ones

Sample_geo_accession	GSM1106068
Sample_status	Public on Mar 28 2013
Sample_submission_date	Mar 26 2013
Sample_last_update_date	Mar 28 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	none
Sample_growth_protocol_ch1	Fibroblast cells were cultured in Eagle's minimum essential (MEM) supplemented with 10% FBS, 100 mg/mL streptomycin, 100 U/mL penicillin and incubated at 37 C in a humidified chamber with 5% CO2. Cells were harvested once they reached confluence by treating with trypsin (0.05% trypsin with 0.25% EDTA).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA followed by Qiagen cleanup were performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 plus Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	The GeneChip was scanned and data extracted using GeneChip scanner 3000 7G (Affimetrix, SantanClara, CA).
Sample_data_processing	The data were analyzed with Expression Console™ Software using Affymetrix default analysis settings and Robust Multichip Analysis (RMA). Subsequently analyses for differentially expressed genes cathegorization were performed with OnechannelGUI in R environment
Sample_platform_id	GPL570
Sample_contact_name	andrea,,bozzato
Sample_contact_email	bzzandrew@yahoo.it
Sample_contact_institute	university
Sample_contact_address	via bassi
Sample_contact_city	padova
Sample_contact_zip/postal_code	35123
Sample_contact_country	Italy
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1106nnn/GSM1106068/suppl/GSM1106068_bb2hd.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1106nnn/GSM1106068/suppl/GSM1106068_bb2hd.rma.chp.gz
Sample_series_id	GSE45516
Sample_data_row_count	54675

GSM1106069

GPL570

hd2

dermal fibroblast

disease: Huntington cell type: Fibroblast cells age: 63 Sex: male

Gene expression profile comparison from fibroblasts of Huntington individuals and normal ones

Sample_geo_accession	GSM1106069
Sample_status	Public on Mar 28 2013
Sample_submission_date	Mar 26 2013
Sample_last_update_date	Mar 28 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	none
Sample_growth_protocol_ch1	Fibroblast cells were cultured in Eagle's minimum essential (MEM) supplemented with 10% FBS, 100 mg/mL streptomycin, 100 U/mL penicillin and incubated at 37 C in a humidified chamber with 5% CO2. Cells were harvested once they reached confluence by treating with trypsin (0.05% trypsin with 0.25% EDTA).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA followed by Qiagen cleanup were performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 plus Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	The GeneChip was scanned and data extracted using GeneChip scanner 3000 7G (Affimetrix, SantanClara, CA).
Sample_data_processing	The data were analyzed with Expression Console™ Software using Affymetrix default analysis settings and Robust Multichip Analysis (RMA). Subsequently analyses for differentially expressed genes cathegorization were performed with OnechannelGUI in R environment
Sample_platform_id	GPL570
Sample_contact_name	andrea,,bozzato
Sample_contact_email	bzzandrew@yahoo.it
Sample_contact_institute	university
Sample_contact_address	via bassi
Sample_contact_city	padova
Sample_contact_zip/postal_code	35123
Sample_contact_country	Italy
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1106nnn/GSM1106069/suppl/GSM1106069_fg1hd.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1106nnn/GSM1106069/suppl/GSM1106069_fg1hd.rma.chp.gz
Sample_series_id	GSE45516
Sample_data_row_count	54675

GSM1106070

GPL570

hd3

dermal fibroblast

disease: Huntington cell type: Fibroblast cells age: 57 Sex: male

Gene expression profile comparison from fibroblasts of Huntington individuals and normal ones

Sample_geo_accession	GSM1106070
Sample_status	Public on Mar 28 2013
Sample_submission_date	Mar 26 2013
Sample_last_update_date	Mar 28 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	none
Sample_growth_protocol_ch1	Fibroblast cells were cultured in Eagle's minimum essential (MEM) supplemented with 10% FBS, 100 mg/mL streptomycin, 100 U/mL penicillin and incubated at 37 C in a humidified chamber with 5% CO2. Cells were harvested once they reached confluence by treating with trypsin (0.05% trypsin with 0.25% EDTA).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA followed by Qiagen cleanup were performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 plus Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	The GeneChip was scanned and data extracted using GeneChip scanner 3000 7G (Affimetrix, SantanClara, CA).
Sample_data_processing	The data were analyzed with Expression Console™ Software using Affymetrix default analysis settings and Robust Multichip Analysis (RMA). Subsequently analyses for differentially expressed genes cathegorization were performed with OnechannelGUI in R environment
Sample_platform_id	GPL570
Sample_contact_name	andrea,,bozzato
Sample_contact_email	bzzandrew@yahoo.it
Sample_contact_institute	university
Sample_contact_address	via bassi
Sample_contact_city	padova
Sample_contact_zip/postal_code	35123
Sample_contact_country	Italy
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1106nnn/GSM1106070/suppl/GSM1106070_lg1.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1106nnn/GSM1106070/suppl/GSM1106070_lg1.rma.chp.gz
Sample_series_id	GSE45516
Sample_data_row_count	54675

GSM1106071

GPL570

hd4

dermal fibroblast

disease: Huntington cell type: Fibroblast cells age: 47 Sex: male

Gene expression profile comparison from fibroblasts of Huntington individuals and normal ones

Sample_geo_accession	GSM1106071
Sample_status	Public on Mar 28 2013
Sample_submission_date	Mar 26 2013
Sample_last_update_date	Mar 28 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	none
Sample_growth_protocol_ch1	Fibroblast cells were cultured in Eagle's minimum essential (MEM) supplemented with 10% FBS, 100 mg/mL streptomycin, 100 U/mL penicillin and incubated at 37 C in a humidified chamber with 5% CO2. Cells were harvested once they reached confluence by treating with trypsin (0.05% trypsin with 0.25% EDTA).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA followed by Qiagen cleanup were performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 plus Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	The GeneChip was scanned and data extracted using GeneChip scanner 3000 7G (Affimetrix, SantanClara, CA).
Sample_data_processing	The data were analyzed with Expression Console™ Software using Affymetrix default analysis settings and Robust Multichip Analysis (RMA). Subsequently analyses for differentially expressed genes cathegorization were performed with OnechannelGUI in R environment
Sample_platform_id	GPL570
Sample_contact_name	andrea,,bozzato
Sample_contact_email	bzzandrew@yahoo.it
Sample_contact_institute	university
Sample_contact_address	via bassi
Sample_contact_city	padova
Sample_contact_zip/postal_code	35123
Sample_contact_country	Italy
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1106nnn/GSM1106071/suppl/GSM1106071_lv1.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1106nnn/GSM1106071/suppl/GSM1106071_lv1.rma.chp.gz
Sample_series_id	GSE45516
Sample_data_row_count	54675

GSM1106072

GPL570

hd5

dermal fibroblast

disease: Huntington cell type: Fibroblast cells age: 38 Sex: male

Gene expression profile comparison from fibroblasts of Huntington individuals and normal ones

Sample_geo_accession	GSM1106072
Sample_status	Public on Mar 28 2013
Sample_submission_date	Mar 26 2013
Sample_last_update_date	Mar 28 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	none
Sample_growth_protocol_ch1	Fibroblast cells were cultured in Eagle's minimum essential (MEM) supplemented with 10% FBS, 100 mg/mL streptomycin, 100 U/mL penicillin and incubated at 37 C in a humidified chamber with 5% CO2. Cells were harvested once they reached confluence by treating with trypsin (0.05% trypsin with 0.25% EDTA).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA followed by Qiagen cleanup were performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 plus Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	The GeneChip was scanned and data extracted using GeneChip scanner 3000 7G (Affimetrix, SantanClara, CA).
Sample_data_processing	The data were analyzed with Expression Console™ Software using Affymetrix default analysis settings and Robust Multichip Analysis (RMA). Subsequently analyses for differentially expressed genes cathegorization were performed with OnechannelGUI in R environment
Sample_platform_id	GPL570
Sample_contact_name	andrea,,bozzato
Sample_contact_email	bzzandrew@yahoo.it
Sample_contact_institute	university
Sample_contact_address	via bassi
Sample_contact_city	padova
Sample_contact_zip/postal_code	35123
Sample_contact_country	Italy
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1106nnn/GSM1106072/suppl/GSM1106072_pf1.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1106nnn/GSM1106072/suppl/GSM1106072_pf1.rma.chp.gz
Sample_series_id	GSE45516
Sample_data_row_count	54675

GSM1106073

GPL570

hd6

dermal fibroblast

disease: Huntington cell type: Fibroblast cells age: 37 Sex: male

Gene expression profile comparison from fibroblasts of Huntington individuals and normal ones

Sample_geo_accession	GSM1106073
Sample_status	Public on Mar 28 2013
Sample_submission_date	Mar 26 2013
Sample_last_update_date	Mar 28 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	none
Sample_growth_protocol_ch1	Fibroblast cells were cultured in Eagle's minimum essential (MEM) supplemented with 10% FBS, 100 mg/mL streptomycin, 100 U/mL penicillin and incubated at 37 C in a humidified chamber with 5% CO2. Cells were harvested once they reached confluence by treating with trypsin (0.05% trypsin with 0.25% EDTA).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA followed by Qiagen cleanup were performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 plus Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	The GeneChip was scanned and data extracted using GeneChip scanner 3000 7G (Affimetrix, SantanClara, CA).
Sample_data_processing	The data were analyzed with Expression Console™ Software using Affymetrix default analysis settings and Robust Multichip Analysis (RMA). Subsequently analyses for differentially expressed genes cathegorization were performed with OnechannelGUI in R environment
Sample_platform_id	GPL570
Sample_contact_name	andrea,,bozzato
Sample_contact_email	bzzandrew@yahoo.it
Sample_contact_institute	university
Sample_contact_address	via bassi
Sample_contact_city	padova
Sample_contact_zip/postal_code	35123
Sample_contact_country	Italy
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1106nnn/GSM1106073/suppl/GSM1106073_4799HD.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1106nnn/GSM1106073/suppl/GSM1106073_4799HD.rma.chp.gz
Sample_series_id	GSE45516
Sample_data_row_count	54675

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