Search results for the GEO ID: GSE45535  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1108138 | GPL570 |
|
Donor-1, Whole blood
|
Whole Blood
|
cell type: Whole Blood
markers: NA
|
HGU133 Plus array data
|
| Sample_geo_accession | GSM1108138
| | Sample_status | Public on Mar 28 2013
| | Sample_submission_date | Mar 27 2013
| | Sample_last_update_date | Mar 28 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To examine gene expression in purified cellular fractions, normal human blood was collected from 2 donors as per institutional policy. The granulocyte (CD15+), monocyte (CD14+), T cell (CD3+), B cell (Non-PC gated, CD19+), and PC (CD27++CD38++) fractions from peripheral blood were separated. White blood cells were washed with FACS buffer (PBS + 0.5%BSA + 2mM EDTA (Gibco)) and incubated with 20% heat-inactivated FBS for 10-15 minutes on ice. The following mAbs were added directly to the cells: CD15 (HI98); CD14 (M5E2), CD3 (UCHT1), CD27 (M-T271), CD38 (HB7), and DAPI (Molecular probes). Cells were sorted on a Becton Dickinson FACS Aria II flow cytometer. All sorted fractions were collected in FACS buffer, centrifuged, and the resulting cell pellet was suspended in RNA lysis buffer (Ambion).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Zymo RNA MicroPrep kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Ambion MessageAmp II-Biotin Enhanced Single Round aRNA Amplification protocol from 75 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HGU133 Plus 2.0 Array. GeneChips were washed and stained in an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data was analyzed in R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,Allen,Morehouse
| | Sample_contact_institute | Medimmune LLC
| | Sample_contact_address | One Medimmune Way
| | Sample_contact_city | Gaithersburg
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20878
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1108nnn/GSM1108138/suppl/GSM1108138_D01_WB.CEL.gz
| | Sample_series_id | GSE45535
| | Sample_series_id | GSE45537
| | Sample_data_row_count | 54675
| | |
|
GSM1108139 | GPL570 |
|
Donor-1, B cells (CD19+CD20+)
|
B cells
|
cell type: B cells
markers: CD19+CD20+
|
HGU133 Plus array data
|
| Sample_geo_accession | GSM1108139
| | Sample_status | Public on Mar 28 2013
| | Sample_submission_date | Mar 27 2013
| | Sample_last_update_date | Mar 28 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To examine gene expression in purified cellular fractions, normal human blood was collected from 2 donors as per institutional policy. The granulocyte (CD15+), monocyte (CD14+), T cell (CD3+), B cell (Non-PC gated, CD19+), and PC (CD27++CD38++) fractions from peripheral blood were separated. White blood cells were washed with FACS buffer (PBS + 0.5%BSA + 2mM EDTA (Gibco)) and incubated with 20% heat-inactivated FBS for 10-15 minutes on ice. The following mAbs were added directly to the cells: CD15 (HI98); CD14 (M5E2), CD3 (UCHT1), CD27 (M-T271), CD38 (HB7), and DAPI (Molecular probes). Cells were sorted on a Becton Dickinson FACS Aria II flow cytometer. All sorted fractions were collected in FACS buffer, centrifuged, and the resulting cell pellet was suspended in RNA lysis buffer (Ambion).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Zymo RNA MicroPrep kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Ambion MessageAmp II-Biotin Enhanced Single Round aRNA Amplification protocol from 75 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HGU133 Plus 2.0 Array. GeneChips were washed and stained in an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data was analyzed in R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,Allen,Morehouse
| | Sample_contact_institute | Medimmune LLC
| | Sample_contact_address | One Medimmune Way
| | Sample_contact_city | Gaithersburg
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20878
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1108nnn/GSM1108139/suppl/GSM1108139_D01_B_cells.CEL.gz
| | Sample_series_id | GSE45535
| | Sample_series_id | GSE45537
| | Sample_data_row_count | 54675
| | |
|
GSM1108140 | GPL570 |
|
Donor-1, Granulocytes (CD15+)
|
Granulocytes
|
cell type: Granulocytes
markers: CD15+
|
HGU133 Plus array data
|
| Sample_geo_accession | GSM1108140
| | Sample_status | Public on Mar 28 2013
| | Sample_submission_date | Mar 27 2013
| | Sample_last_update_date | Mar 28 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To examine gene expression in purified cellular fractions, normal human blood was collected from 2 donors as per institutional policy. The granulocyte (CD15+), monocyte (CD14+), T cell (CD3+), B cell (Non-PC gated, CD19+), and PC (CD27++CD38++) fractions from peripheral blood were separated. White blood cells were washed with FACS buffer (PBS + 0.5%BSA + 2mM EDTA (Gibco)) and incubated with 20% heat-inactivated FBS for 10-15 minutes on ice. The following mAbs were added directly to the cells: CD15 (HI98); CD14 (M5E2), CD3 (UCHT1), CD27 (M-T271), CD38 (HB7), and DAPI (Molecular probes). Cells were sorted on a Becton Dickinson FACS Aria II flow cytometer. All sorted fractions were collected in FACS buffer, centrifuged, and the resulting cell pellet was suspended in RNA lysis buffer (Ambion).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Zymo RNA MicroPrep kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Ambion MessageAmp II-Biotin Enhanced Single Round aRNA Amplification protocol from 75 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HGU133 Plus 2.0 Array. GeneChips were washed and stained in an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data was analyzed in R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,Allen,Morehouse
| | Sample_contact_institute | Medimmune LLC
| | Sample_contact_address | One Medimmune Way
| | Sample_contact_city | Gaithersburg
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20878
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1108nnn/GSM1108140/suppl/GSM1108140_D01_Granulocytes.CEL.gz
| | Sample_series_id | GSE45535
| | Sample_series_id | GSE45537
| | Sample_data_row_count | 54675
| | |
|
GSM1108141 | GPL570 |
|
Donor-1, Monocytes (CD14+)
|
Monocytes
|
cell type: Monocytes
markers: CD14+
|
HGU133 Plus array data
|
| Sample_geo_accession | GSM1108141
| | Sample_status | Public on Mar 28 2013
| | Sample_submission_date | Mar 27 2013
| | Sample_last_update_date | Mar 28 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To examine gene expression in purified cellular fractions, normal human blood was collected from 2 donors as per institutional policy. The granulocyte (CD15+), monocyte (CD14+), T cell (CD3+), B cell (Non-PC gated, CD19+), and PC (CD27++CD38++) fractions from peripheral blood were separated. White blood cells were washed with FACS buffer (PBS + 0.5%BSA + 2mM EDTA (Gibco)) and incubated with 20% heat-inactivated FBS for 10-15 minutes on ice. The following mAbs were added directly to the cells: CD15 (HI98); CD14 (M5E2), CD3 (UCHT1), CD27 (M-T271), CD38 (HB7), and DAPI (Molecular probes). Cells were sorted on a Becton Dickinson FACS Aria II flow cytometer. All sorted fractions were collected in FACS buffer, centrifuged, and the resulting cell pellet was suspended in RNA lysis buffer (Ambion).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Zymo RNA MicroPrep kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Ambion MessageAmp II-Biotin Enhanced Single Round aRNA Amplification protocol from 75 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HGU133 Plus 2.0 Array. GeneChips were washed and stained in an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data was analyzed in R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,Allen,Morehouse
| | Sample_contact_institute | Medimmune LLC
| | Sample_contact_address | One Medimmune Way
| | Sample_contact_city | Gaithersburg
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20878
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1108nnn/GSM1108141/suppl/GSM1108141_D01_Monocytes.CEL.gz
| | Sample_series_id | GSE45535
| | Sample_series_id | GSE45537
| | Sample_data_row_count | 54675
| | |
|
GSM1108142 | GPL570 |
|
Donor-1, Plasma cells (CD38+CD27+)
|
Plasma cells
|
cell type: Plasma cells
markers: CD38+CD27+
|
HGU133 Plus array data
|
| Sample_geo_accession | GSM1108142
| | Sample_status | Public on Mar 28 2013
| | Sample_submission_date | Mar 27 2013
| | Sample_last_update_date | Mar 28 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To examine gene expression in purified cellular fractions, normal human blood was collected from 2 donors as per institutional policy. The granulocyte (CD15+), monocyte (CD14+), T cell (CD3+), B cell (Non-PC gated, CD19+), and PC (CD27++CD38++) fractions from peripheral blood were separated. White blood cells were washed with FACS buffer (PBS + 0.5%BSA + 2mM EDTA (Gibco)) and incubated with 20% heat-inactivated FBS for 10-15 minutes on ice. The following mAbs were added directly to the cells: CD15 (HI98); CD14 (M5E2), CD3 (UCHT1), CD27 (M-T271), CD38 (HB7), and DAPI (Molecular probes). Cells were sorted on a Becton Dickinson FACS Aria II flow cytometer. All sorted fractions were collected in FACS buffer, centrifuged, and the resulting cell pellet was suspended in RNA lysis buffer (Ambion).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Zymo RNA MicroPrep kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Ambion MessageAmp II-Biotin Enhanced Single Round aRNA Amplification protocol from 75 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HGU133 Plus 2.0 Array. GeneChips were washed and stained in an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data was analyzed in R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,Allen,Morehouse
| | Sample_contact_institute | Medimmune LLC
| | Sample_contact_address | One Medimmune Way
| | Sample_contact_city | Gaithersburg
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20878
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1108nnn/GSM1108142/suppl/GSM1108142_D01_PC.CEL.gz
| | Sample_series_id | GSE45535
| | Sample_series_id | GSE45537
| | Sample_data_row_count | 54675
| | |
|
GSM1108143 | GPL570 |
|
Donor-1, T cells (CD3+)
|
T cells
|
cell type: T cells
markers: CD3+
|
HGU133 Plus array data
|
| Sample_geo_accession | GSM1108143
| | Sample_status | Public on Mar 28 2013
| | Sample_submission_date | Mar 27 2013
| | Sample_last_update_date | Mar 28 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To examine gene expression in purified cellular fractions, normal human blood was collected from 2 donors as per institutional policy. The granulocyte (CD15+), monocyte (CD14+), T cell (CD3+), B cell (Non-PC gated, CD19+), and PC (CD27++CD38++) fractions from peripheral blood were separated. White blood cells were washed with FACS buffer (PBS + 0.5%BSA + 2mM EDTA (Gibco)) and incubated with 20% heat-inactivated FBS for 10-15 minutes on ice. The following mAbs were added directly to the cells: CD15 (HI98); CD14 (M5E2), CD3 (UCHT1), CD27 (M-T271), CD38 (HB7), and DAPI (Molecular probes). Cells were sorted on a Becton Dickinson FACS Aria II flow cytometer. All sorted fractions were collected in FACS buffer, centrifuged, and the resulting cell pellet was suspended in RNA lysis buffer (Ambion).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Zymo RNA MicroPrep kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Ambion MessageAmp II-Biotin Enhanced Single Round aRNA Amplification protocol from 75 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HGU133 Plus 2.0 Array. GeneChips were washed and stained in an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data was analyzed in R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,Allen,Morehouse
| | Sample_contact_institute | Medimmune LLC
| | Sample_contact_address | One Medimmune Way
| | Sample_contact_city | Gaithersburg
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20878
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1108nnn/GSM1108143/suppl/GSM1108143_D01_T_cells.CEL.gz
| | Sample_series_id | GSE45535
| | Sample_series_id | GSE45537
| | Sample_data_row_count | 54675
| | |
|
GSM1108144 | GPL570 |
|
Donor-2, Whole blood
|
Whole Blood
|
cell type: Whole Blood
markers: NA
|
HGU133 Plus array data
|
| Sample_geo_accession | GSM1108144
| | Sample_status | Public on Mar 28 2013
| | Sample_submission_date | Mar 27 2013
| | Sample_last_update_date | Mar 28 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To examine gene expression in purified cellular fractions, normal human blood was collected from 2 donors as per institutional policy. The granulocyte (CD15+), monocyte (CD14+), T cell (CD3+), B cell (Non-PC gated, CD19+), and PC (CD27++CD38++) fractions from peripheral blood were separated. White blood cells were washed with FACS buffer (PBS + 0.5%BSA + 2mM EDTA (Gibco)) and incubated with 20% heat-inactivated FBS for 10-15 minutes on ice. The following mAbs were added directly to the cells: CD15 (HI98); CD14 (M5E2), CD3 (UCHT1), CD27 (M-T271), CD38 (HB7), and DAPI (Molecular probes). Cells were sorted on a Becton Dickinson FACS Aria II flow cytometer. All sorted fractions were collected in FACS buffer, centrifuged, and the resulting cell pellet was suspended in RNA lysis buffer (Ambion).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Zymo RNA MicroPrep kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Ambion MessageAmp II-Biotin Enhanced Single Round aRNA Amplification protocol from 75 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HGU133 Plus 2.0 Array. GeneChips were washed and stained in an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data was analyzed in R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,Allen,Morehouse
| | Sample_contact_institute | Medimmune LLC
| | Sample_contact_address | One Medimmune Way
| | Sample_contact_city | Gaithersburg
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20878
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1108nnn/GSM1108144/suppl/GSM1108144_D02_WB.CEL.gz
| | Sample_series_id | GSE45535
| | Sample_series_id | GSE45537
| | Sample_data_row_count | 54675
| | |
|
GSM1108145 | GPL570 |
|
Donor-2, B cells (CD19+CD20+)
|
B cells
|
cell type: B cells
markers: CD19+CD20+
|
HGU133 Plus array data
|
| Sample_geo_accession | GSM1108145
| | Sample_status | Public on Mar 28 2013
| | Sample_submission_date | Mar 27 2013
| | Sample_last_update_date | Mar 28 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To examine gene expression in purified cellular fractions, normal human blood was collected from 2 donors as per institutional policy. The granulocyte (CD15+), monocyte (CD14+), T cell (CD3+), B cell (Non-PC gated, CD19+), and PC (CD27++CD38++) fractions from peripheral blood were separated. White blood cells were washed with FACS buffer (PBS + 0.5%BSA + 2mM EDTA (Gibco)) and incubated with 20% heat-inactivated FBS for 10-15 minutes on ice. The following mAbs were added directly to the cells: CD15 (HI98); CD14 (M5E2), CD3 (UCHT1), CD27 (M-T271), CD38 (HB7), and DAPI (Molecular probes). Cells were sorted on a Becton Dickinson FACS Aria II flow cytometer. All sorted fractions were collected in FACS buffer, centrifuged, and the resulting cell pellet was suspended in RNA lysis buffer (Ambion).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Zymo RNA MicroPrep kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Ambion MessageAmp II-Biotin Enhanced Single Round aRNA Amplification protocol from 75 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HGU133 Plus 2.0 Array. GeneChips were washed and stained in an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data was analyzed in R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,Allen,Morehouse
| | Sample_contact_institute | Medimmune LLC
| | Sample_contact_address | One Medimmune Way
| | Sample_contact_city | Gaithersburg
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20878
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1108nnn/GSM1108145/suppl/GSM1108145_D02_B_cells.CEL.gz
| | Sample_series_id | GSE45535
| | Sample_series_id | GSE45537
| | Sample_data_row_count | 54675
| | |
|
GSM1108146 | GPL570 |
|
Donor-2, Granulocytes (CD15+)
|
Granulocytes
|
cell type: Granulocytes
markers: CD15+
|
HGU133 Plus array data
|
| Sample_geo_accession | GSM1108146
| | Sample_status | Public on Mar 28 2013
| | Sample_submission_date | Mar 27 2013
| | Sample_last_update_date | Mar 28 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To examine gene expression in purified cellular fractions, normal human blood was collected from 2 donors as per institutional policy. The granulocyte (CD15+), monocyte (CD14+), T cell (CD3+), B cell (Non-PC gated, CD19+), and PC (CD27++CD38++) fractions from peripheral blood were separated. White blood cells were washed with FACS buffer (PBS + 0.5%BSA + 2mM EDTA (Gibco)) and incubated with 20% heat-inactivated FBS for 10-15 minutes on ice. The following mAbs were added directly to the cells: CD15 (HI98); CD14 (M5E2), CD3 (UCHT1), CD27 (M-T271), CD38 (HB7), and DAPI (Molecular probes). Cells were sorted on a Becton Dickinson FACS Aria II flow cytometer. All sorted fractions were collected in FACS buffer, centrifuged, and the resulting cell pellet was suspended in RNA lysis buffer (Ambion).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Zymo RNA MicroPrep kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Ambion MessageAmp II-Biotin Enhanced Single Round aRNA Amplification protocol from 75 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HGU133 Plus 2.0 Array. GeneChips were washed and stained in an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data was analyzed in R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,Allen,Morehouse
| | Sample_contact_institute | Medimmune LLC
| | Sample_contact_address | One Medimmune Way
| | Sample_contact_city | Gaithersburg
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20878
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1108nnn/GSM1108146/suppl/GSM1108146_D02_Granulocytes.CEL.gz
| | Sample_series_id | GSE45535
| | Sample_series_id | GSE45537
| | Sample_data_row_count | 54675
| | |
|
GSM1108147 | GPL570 |
|
Donor-2, Monocytes (CD14+)
|
Monocytes
|
cell type: Monocytes
markers: CD14+
|
HGU133 Plus array data
|
| Sample_geo_accession | GSM1108147
| | Sample_status | Public on Mar 28 2013
| | Sample_submission_date | Mar 27 2013
| | Sample_last_update_date | Mar 28 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To examine gene expression in purified cellular fractions, normal human blood was collected from 2 donors as per institutional policy. The granulocyte (CD15+), monocyte (CD14+), T cell (CD3+), B cell (Non-PC gated, CD19+), and PC (CD27++CD38++) fractions from peripheral blood were separated. White blood cells were washed with FACS buffer (PBS + 0.5%BSA + 2mM EDTA (Gibco)) and incubated with 20% heat-inactivated FBS for 10-15 minutes on ice. The following mAbs were added directly to the cells: CD15 (HI98); CD14 (M5E2), CD3 (UCHT1), CD27 (M-T271), CD38 (HB7), and DAPI (Molecular probes). Cells were sorted on a Becton Dickinson FACS Aria II flow cytometer. All sorted fractions were collected in FACS buffer, centrifuged, and the resulting cell pellet was suspended in RNA lysis buffer (Ambion).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Zymo RNA MicroPrep kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Ambion MessageAmp II-Biotin Enhanced Single Round aRNA Amplification protocol from 75 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HGU133 Plus 2.0 Array. GeneChips were washed and stained in an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data was analyzed in R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,Allen,Morehouse
| | Sample_contact_institute | Medimmune LLC
| | Sample_contact_address | One Medimmune Way
| | Sample_contact_city | Gaithersburg
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20878
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1108nnn/GSM1108147/suppl/GSM1108147_D02_Monocytes.CEL.gz
| | Sample_series_id | GSE45535
| | Sample_series_id | GSE45537
| | Sample_data_row_count | 54675
| | |
|
GSM1108148 | GPL570 |
|
Donor-2, Plasma cells (CD38+CD27+)
|
Plasma cells
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cell type: Plasma cells
markers: CD38+CD27+
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HGU133 Plus array data
|
| Sample_geo_accession | GSM1108148
| | Sample_status | Public on Mar 28 2013
| | Sample_submission_date | Mar 27 2013
| | Sample_last_update_date | Mar 28 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To examine gene expression in purified cellular fractions, normal human blood was collected from 2 donors as per institutional policy. The granulocyte (CD15+), monocyte (CD14+), T cell (CD3+), B cell (Non-PC gated, CD19+), and PC (CD27++CD38++) fractions from peripheral blood were separated. White blood cells were washed with FACS buffer (PBS + 0.5%BSA + 2mM EDTA (Gibco)) and incubated with 20% heat-inactivated FBS for 10-15 minutes on ice. The following mAbs were added directly to the cells: CD15 (HI98); CD14 (M5E2), CD3 (UCHT1), CD27 (M-T271), CD38 (HB7), and DAPI (Molecular probes). Cells were sorted on a Becton Dickinson FACS Aria II flow cytometer. All sorted fractions were collected in FACS buffer, centrifuged, and the resulting cell pellet was suspended in RNA lysis buffer (Ambion).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Zymo RNA MicroPrep kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Ambion MessageAmp II-Biotin Enhanced Single Round aRNA Amplification protocol from 75 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HGU133 Plus 2.0 Array. GeneChips were washed and stained in an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data was analyzed in R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,Allen,Morehouse
| | Sample_contact_institute | Medimmune LLC
| | Sample_contact_address | One Medimmune Way
| | Sample_contact_city | Gaithersburg
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20878
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1108nnn/GSM1108148/suppl/GSM1108148_D02_PC.CEL.gz
| | Sample_series_id | GSE45535
| | Sample_series_id | GSE45537
| | Sample_data_row_count | 54675
| | |
|
GSM1108149 | GPL570 |
|
Donor-2, T cells (CD3+)
|
T cells
|
cell type: T cells
markers: CD3+
|
HGU133 Plus array data
|
| Sample_geo_accession | GSM1108149
| | Sample_status | Public on Mar 28 2013
| | Sample_submission_date | Mar 27 2013
| | Sample_last_update_date | Mar 28 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To examine gene expression in purified cellular fractions, normal human blood was collected from 2 donors as per institutional policy. The granulocyte (CD15+), monocyte (CD14+), T cell (CD3+), B cell (Non-PC gated, CD19+), and PC (CD27++CD38++) fractions from peripheral blood were separated. White blood cells were washed with FACS buffer (PBS + 0.5%BSA + 2mM EDTA (Gibco)) and incubated with 20% heat-inactivated FBS for 10-15 minutes on ice. The following mAbs were added directly to the cells: CD15 (HI98); CD14 (M5E2), CD3 (UCHT1), CD27 (M-T271), CD38 (HB7), and DAPI (Molecular probes). Cells were sorted on a Becton Dickinson FACS Aria II flow cytometer. All sorted fractions were collected in FACS buffer, centrifuged, and the resulting cell pellet was suspended in RNA lysis buffer (Ambion).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Zymo RNA MicroPrep kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Ambion MessageAmp II-Biotin Enhanced Single Round aRNA Amplification protocol from 75 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HGU133 Plus 2.0 Array. GeneChips were washed and stained in an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data was analyzed in R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Christopher,Allen,Morehouse
| | Sample_contact_institute | Medimmune LLC
| | Sample_contact_address | One Medimmune Way
| | Sample_contact_city | Gaithersburg
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20878
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1108nnn/GSM1108149/suppl/GSM1108149_D02_T_cells.CEL.gz
| | Sample_series_id | GSE45535
| | Sample_series_id | GSE45537
| | Sample_data_row_count | 54675
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