Search results for the GEO ID: GSE45566  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1109799 | GPL1261 |
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GFP
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Gene-transduced LSK Flt3- cells, GFP control
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strain: C57BL6J
genotype: wild-type
cell type: LSK Flt3- cells
transduction: GFP
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Gene expression data from gene-transduced LSK Flt3- cells.
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| Sample_geo_accession | GSM1109799
| | Sample_status | Public on Apr 01 2013
| | Sample_submission_date | Mar 27 2013
| | Sample_last_update_date | Apr 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using a Trizol Reagent according to the manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were synthesized by the GeneChip 3' IVT Express Kit (Affymetrix) from 250 ng total RNA according to the manufacturer's instructions. Biotinylated cRNA yield were checked with the NanoDrop ND-2000 Spectrophotometer.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 degrees on GeneChip Mouse Genome 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G.
| | Sample_data_processing | Single Array Analysis were calculated by Microarray Suite version 5.0 (MAS5.0) with Affymetrix default settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yusuke,,Satoh
| | Sample_contact_email | ysatoh@bldon.med.osaka-u.ac.jp
| | Sample_contact_department | Department of Hematology and Oncology
| | Sample_contact_institute | Osaka University Graduate School of Medicine
| | Sample_contact_address | 2-2, Yamada-Oka
| | Sample_contact_city | Suita
| | Sample_contact_state | Osaka
| | Sample_contact_zip/postal_code | 565-0871
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1109nnn/GSM1109799/suppl/GSM1109799_EA0775_01.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1109nnn/GSM1109799/suppl/GSM1109799_EA0775_01_scale.CHP.gz
| | Sample_series_id | GSE45566
| | Sample_data_row_count | 45037
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GSM1109800 | GPL1261 |
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Satb1
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Gene-transduced LSK Flt3- cells, Satb1
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strain: C57BL6J
genotype: wild-type
cell type: LSK Flt3- cells
transduction: Satb1
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Gene expression data from gene-transduced LSK Flt3- cells.
|
| Sample_geo_accession | GSM1109800
| | Sample_status | Public on Apr 01 2013
| | Sample_submission_date | Mar 27 2013
| | Sample_last_update_date | Apr 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using a Trizol Reagent according to the manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were synthesized by the GeneChip 3' IVT Express Kit (Affymetrix) from 250 ng total RNA according to the manufacturer's instructions. Biotinylated cRNA yield were checked with the NanoDrop ND-2000 Spectrophotometer.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 degrees on GeneChip Mouse Genome 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G.
| | Sample_data_processing | Single Array Analysis were calculated by Microarray Suite version 5.0 (MAS5.0) with Affymetrix default settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yusuke,,Satoh
| | Sample_contact_email | ysatoh@bldon.med.osaka-u.ac.jp
| | Sample_contact_department | Department of Hematology and Oncology
| | Sample_contact_institute | Osaka University Graduate School of Medicine
| | Sample_contact_address | 2-2, Yamada-Oka
| | Sample_contact_city | Suita
| | Sample_contact_state | Osaka
| | Sample_contact_zip/postal_code | 565-0871
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1109nnn/GSM1109800/suppl/GSM1109800_EA0775_02.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1109nnn/GSM1109800/suppl/GSM1109800_EA0775_02_scale.CHP.gz
| | Sample_series_id | GSE45566
| | Sample_data_row_count | 45037
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