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Search results for the GEO ID: GSE45665

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Description

Characteristics

GSM1111504

GPL570

RA-FLS_sample1_stimulated by control IgG1

Fibroblast like synoviocytes from patient with rheumatoid arthritis, sample1

tissue: Fibroblast like synoviocytes ethnicity: Asian

Gene expression data from RA-FLS sample1 stimulated by control IgG1

Sample_geo_accession	GSM1111504
Sample_status	Public on Apr 02 2013
Sample_submission_date	Apr 01 2013
Sample_last_update_date	Apr 02 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	RA-FLS were incubated with 1.0 μg/mL of recombinant human DcR3-Fc protein or control human IgG1 (R&D Systems, Minneapolis, MN) for 12 hours at 37°C with 5% CO2.
Sample_growth_protocol_ch1	RA-FLS were cultured in Dulbecco’s modified Eagle’s medium (Gibco BRL, Grand Island, NY) supplemented with 10% fetal bovine serum (BioWhittaker, Walkersville, MD) and 100 units/mL of penicillin/streptomycin at 37°C with 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted with a QIAshredder and RNeasy Mini kit (Qiagen, Hilden, Germany) according to the manufacture’s protocol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA (3' IVT Express Kit User Manual, 2008, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 with Hybridization, Wash and Stain Kit.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GCS3000 scanner.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 500.
Sample_platform_id	GPL570
Sample_contact_name	Koji,,Fukuda
Sample_contact_department	Department of Orthopaedic Surgery
Sample_contact_institute	Kobe University Graduate School of Medicine
Sample_contact_address	7-5-1 Kusunoki, Chuo
Sample_contact_city	Kobe
Sample_contact_zip/postal_code	650-0017
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1111nnn/GSM1111504/suppl/GSM1111504_RA-FLS_sample1_contorol.CEL.gz
Sample_series_id	GSE45665
Sample_data_row_count	54675

GSM1111505

GPL570

RA-FLS_sample1_stimulated by DcR3-Fc

Fibroblast like synoviosytes from patient with rheumatoid arthritis, sample1, stimulated

tissue: Fibroblast like synoviocytes ethnicity: Asian

Gene expression data from RA-FLS sample1 stimulated by human DcR3-Fc

Sample_geo_accession	GSM1111505
Sample_status	Public on Apr 02 2013
Sample_submission_date	Apr 01 2013
Sample_last_update_date	Apr 02 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	RA-FLS were incubated with 1.0 μg/mL of recombinant human DcR3-Fc protein or control human IgG1 (R&D Systems, Minneapolis, MN) for 12 hours at 37°C with 5% CO2.
Sample_growth_protocol_ch1	RA-FLS were cultured in Dulbecco’s modified Eagle’s medium (Gibco BRL, Grand Island, NY) supplemented with 10% fetal bovine serum (BioWhittaker, Walkersville, MD) and 100 units/mL of penicillin/streptomycin at 37°C with 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted with a QIAshredder and RNeasy Mini kit (Qiagen, Hilden, Germany) according to the manufacture’s protocol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA (3' IVT Express Kit User Manual, 2008, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 with Hybridization, Wash and Stain Kit.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GCS3000 scanner.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 500.
Sample_platform_id	GPL570
Sample_contact_name	Koji,,Fukuda
Sample_contact_department	Department of Orthopaedic Surgery
Sample_contact_institute	Kobe University Graduate School of Medicine
Sample_contact_address	7-5-1 Kusunoki, Chuo
Sample_contact_city	Kobe
Sample_contact_zip/postal_code	650-0017
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1111nnn/GSM1111505/suppl/GSM1111505_RA-FLS_sample1_DcR3.CEL.gz
Sample_series_id	GSE45665
Sample_data_row_count	54675

GSM1111506

GPL570

RA-FLS_sample2_stimulated by control IgG1

Fibroblast like synoviocytes from patient with rheumatoid arthritis, sample2

tissue: Fibroblast like synoviocytes ethnicity: Asian

Gene expression data from RA-FLS sample2 stimulated by control IgG1

Sample_geo_accession	GSM1111506
Sample_status	Public on Apr 02 2013
Sample_submission_date	Apr 01 2013
Sample_last_update_date	Apr 02 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	RA-FLS were incubated with 1.0 μg/mL of recombinant human DcR3-Fc protein or control human IgG1 (R&D Systems, Minneapolis, MN) for 12 hours at 37°C with 5% CO2.
Sample_growth_protocol_ch1	RA-FLS were cultured in Dulbecco’s modified Eagle’s medium (Gibco BRL, Grand Island, NY) supplemented with 10% fetal bovine serum (BioWhittaker, Walkersville, MD) and 100 units/mL of penicillin/streptomycin at 37°C with 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted with a QIAshredder and RNeasy Mini kit (Qiagen, Hilden, Germany) according to the manufacture’s protocol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA (3' IVT Express Kit User Manual, 2008, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 with Hybridization, Wash and Stain Kit.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GCS3000 scanner.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 500.
Sample_platform_id	GPL570
Sample_contact_name	Koji,,Fukuda
Sample_contact_department	Department of Orthopaedic Surgery
Sample_contact_institute	Kobe University Graduate School of Medicine
Sample_contact_address	7-5-1 Kusunoki, Chuo
Sample_contact_city	Kobe
Sample_contact_zip/postal_code	650-0017
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1111nnn/GSM1111506/suppl/GSM1111506_RA-FLS_sample2_contorol.CEL.gz
Sample_series_id	GSE45665
Sample_data_row_count	54675

GSM1111507

GPL570

RA-FLS_sample2_stimulated by DcR3-Fc

Fibroblast like synoviosytes from patient with rheumatoid arthritis, sample2, stimulated

tissue: Fibroblast like synoviocytes ethnicity: Asian

Gene expression data from RA-FLS sample2 stimulated by human DcR3-Fc

Sample_geo_accession	GSM1111507
Sample_status	Public on Apr 02 2013
Sample_submission_date	Apr 01 2013
Sample_last_update_date	Apr 02 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	RA-FLS were incubated with 1.0 μg/mL of recombinant human DcR3-Fc protein or control human IgG1 (R&D Systems, Minneapolis, MN) for 12 hours at 37°C with 5% CO2.
Sample_growth_protocol_ch1	RA-FLS were cultured in Dulbecco’s modified Eagle’s medium (Gibco BRL, Grand Island, NY) supplemented with 10% fetal bovine serum (BioWhittaker, Walkersville, MD) and 100 units/mL of penicillin/streptomycin at 37°C with 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted with a QIAshredder and RNeasy Mini kit (Qiagen, Hilden, Germany) according to the manufacture’s protocol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA (3' IVT Express Kit User Manual, 2008, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 with Hybridization, Wash and Stain Kit.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GCS3000 scanner.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 500.
Sample_platform_id	GPL570
Sample_contact_name	Koji,,Fukuda
Sample_contact_department	Department of Orthopaedic Surgery
Sample_contact_institute	Kobe University Graduate School of Medicine
Sample_contact_address	7-5-1 Kusunoki, Chuo
Sample_contact_city	Kobe
Sample_contact_zip/postal_code	650-0017
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1111nnn/GSM1111507/suppl/GSM1111507_RA-FLS_sample2_DcR3.CEL.gz
Sample_series_id	GSE45665
Sample_data_row_count	54675

GSM1111508

GPL570

RA-FLS_sample3_stimulated by control IgG1

Fibroblast like synoviocytes from patient with rheumatoid arthritis, sample3

tissue: Fibroblast like synoviocytes ethnicity: Asian

Gene expression data from RA-FLS sample3 stimulated by control IgG1

Sample_geo_accession	GSM1111508
Sample_status	Public on Apr 02 2013
Sample_submission_date	Apr 01 2013
Sample_last_update_date	Apr 02 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	RA-FLS were incubated with 1.0 μg/mL of recombinant human DcR3-Fc protein or control human IgG1 (R&D Systems, Minneapolis, MN) for 12 hours at 37°C with 5% CO2.
Sample_growth_protocol_ch1	RA-FLS were cultured in Dulbecco’s modified Eagle’s medium (Gibco BRL, Grand Island, NY) supplemented with 10% fetal bovine serum (BioWhittaker, Walkersville, MD) and 100 units/mL of penicillin/streptomycin at 37°C with 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted with a QIAshredder and RNeasy Mini kit (Qiagen, Hilden, Germany) according to the manufacture’s protocol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA (3' IVT Express Kit User Manual, 2008, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 with Hybridization, Wash and Stain Kit.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GCS3000 scanner.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 500.
Sample_platform_id	GPL570
Sample_contact_name	Koji,,Fukuda
Sample_contact_department	Department of Orthopaedic Surgery
Sample_contact_institute	Kobe University Graduate School of Medicine
Sample_contact_address	7-5-1 Kusunoki, Chuo
Sample_contact_city	Kobe
Sample_contact_zip/postal_code	650-0017
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1111nnn/GSM1111508/suppl/GSM1111508_RA-FLS_sample3_contorol.CEL.gz
Sample_series_id	GSE45665
Sample_data_row_count	54675

GSM1111509

GPL570

RA-FLS_sample3_stimulated by DcR3-Fc

Fibroblast like synoviosytes from patient with rheumatoid arthritis, sample3, stimulated

tissue: Fibroblast like synoviocytes ethnicity: Asian

Gene expression data from RA-FLS sample3 stimulated by human DcR3-Fc

Sample_geo_accession	GSM1111509
Sample_status	Public on Apr 02 2013
Sample_submission_date	Apr 01 2013
Sample_last_update_date	Apr 02 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	RA-FLS were incubated with 1.0 μg/mL of recombinant human DcR3-Fc protein or control human IgG1 (R&D Systems, Minneapolis, MN) for 12 hours at 37°C with 5% CO2.
Sample_growth_protocol_ch1	RA-FLS were cultured in Dulbecco’s modified Eagle’s medium (Gibco BRL, Grand Island, NY) supplemented with 10% fetal bovine serum (BioWhittaker, Walkersville, MD) and 100 units/mL of penicillin/streptomycin at 37°C with 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted with a QIAshredder and RNeasy Mini kit (Qiagen, Hilden, Germany) according to the manufacture’s protocol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA (3' IVT Express Kit User Manual, 2008, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 with Hybridization, Wash and Stain Kit.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GCS3000 scanner.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 500.
Sample_platform_id	GPL570
Sample_contact_name	Koji,,Fukuda
Sample_contact_department	Department of Orthopaedic Surgery
Sample_contact_institute	Kobe University Graduate School of Medicine
Sample_contact_address	7-5-1 Kusunoki, Chuo
Sample_contact_city	Kobe
Sample_contact_zip/postal_code	650-0017
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1111nnn/GSM1111509/suppl/GSM1111509_RA-FLS_sample3_DcR3.CEL.gz
Sample_series_id	GSE45665
Sample_data_row_count	54675

GSM1111510

GPL570

RA-FLS_sample4_stimulated by control IgG1

Fibroblast like synoviocytes from patient with rheumatoid arthritis, sample4

tissue: Fibroblast like synoviocytes ethnicity: Asian

Gene expression data from RA-FLS sample4 stimulated by control IgG1

Sample_geo_accession	GSM1111510
Sample_status	Public on Apr 02 2013
Sample_submission_date	Apr 01 2013
Sample_last_update_date	Apr 02 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	RA-FLS were incubated with 1.0 μg/mL of recombinant human DcR3-Fc protein or control human IgG1 (R&D Systems, Minneapolis, MN) for 12 hours at 37°C with 5% CO2.
Sample_growth_protocol_ch1	RA-FLS were cultured in Dulbecco’s modified Eagle’s medium (Gibco BRL, Grand Island, NY) supplemented with 10% fetal bovine serum (BioWhittaker, Walkersville, MD) and 100 units/mL of penicillin/streptomycin at 37°C with 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted with a QIAshredder and RNeasy Mini kit (Qiagen, Hilden, Germany) according to the manufacture’s protocol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA (3' IVT Express Kit User Manual, 2008, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 with Hybridization, Wash and Stain Kit.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GCS3000 scanner.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 500.
Sample_platform_id	GPL570
Sample_contact_name	Koji,,Fukuda
Sample_contact_department	Department of Orthopaedic Surgery
Sample_contact_institute	Kobe University Graduate School of Medicine
Sample_contact_address	7-5-1 Kusunoki, Chuo
Sample_contact_city	Kobe
Sample_contact_zip/postal_code	650-0017
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1111nnn/GSM1111510/suppl/GSM1111510_RA-FLS_sample4_contorol.CEL.gz
Sample_series_id	GSE45665
Sample_data_row_count	54675

GSM1111511

GPL570

RA-FLS_sample4_stimulated by DcR3-Fc

Fibroblast like synoviosytes from patient with rheumatoid arthritis, sample4, stimulated

tissue: Fibroblast like synoviocytes ethnicity: Asian

Gene expression data from RA-FLS sample4 stimulated by human DcR3-Fc

Sample_geo_accession	GSM1111511
Sample_status	Public on Apr 02 2013
Sample_submission_date	Apr 01 2013
Sample_last_update_date	Apr 02 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	RA-FLS were incubated with 1.0 μg/mL of recombinant human DcR3-Fc protein or control human IgG1 (R&D Systems, Minneapolis, MN) for 12 hours at 37°C with 5% CO2.
Sample_growth_protocol_ch1	RA-FLS were cultured in Dulbecco’s modified Eagle’s medium (Gibco BRL, Grand Island, NY) supplemented with 10% fetal bovine serum (BioWhittaker, Walkersville, MD) and 100 units/mL of penicillin/streptomycin at 37°C with 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted with a QIAshredder and RNeasy Mini kit (Qiagen, Hilden, Germany) according to the manufacture’s protocol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA (3' IVT Express Kit User Manual, 2008, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 with Hybridization, Wash and Stain Kit.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GCS3000 scanner.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 500.
Sample_platform_id	GPL570
Sample_contact_name	Koji,,Fukuda
Sample_contact_department	Department of Orthopaedic Surgery
Sample_contact_institute	Kobe University Graduate School of Medicine
Sample_contact_address	7-5-1 Kusunoki, Chuo
Sample_contact_city	Kobe
Sample_contact_zip/postal_code	650-0017
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1111nnn/GSM1111511/suppl/GSM1111511_RA-FLS_sample4_DcR3.CEL.gz
Sample_series_id	GSE45665
Sample_data_row_count	54675

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