Result

Search results for the GEO ID: GSE45668

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM1111640

GPL1261

GV oocytes, XX, biological rep1

Mouse GV oocytes (XX)

genotype: XX cell type: Oocytes Stage: germinal vesicle (GV)

Gene expression from Mouse GV oocytes

Sample_geo_accession	GSM1111640
Sample_status	Public on Apr 02 2013
Sample_submission_date	Apr 01 2013
Sample_last_update_date	Apr 02 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Ovaries were treated with 0.5 mg/ml each of testicular hyaluronidase (type IV), collagenase (B grade) and egg white lysozyme (all from Sigma) in M2 medium as previously reported. Oocytes with 40–60 µm diameter were collected with a fine glass needle, transferred in a group into a microcentrifuge tube, and stored at −80°C
Sample_growth_protocol_ch1	XX, XY and XO females at 25–29 dpp were injected intraperitoneally each with 5 IU equine chorionic gonadotropin (Sigma, St Louis, MO), and sacrificed 45–47 hours later. Fully grown GV-stage oocytes surrounded by cumulus cells were isolated by puncturing large antral follicles with a pair of 26-gauge needles, and then denuded of cumulus cells by repeated pipetting through a fine glass needle. Only the oocytes with an intact GV and no apparent sign of degeneration were collected and stored at −80°C. Growing oocytes were collected from XX, XY, and XO females at 10 and 17 dpp.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA were isolated from 10 oocytes each from 3 XY and 3 XX females using the RNeasy Micro Kit (Qiagen, Valencia, CA) and extracted in 11 µl RNase-free water. The Two-Cycle Eukaryotic Target Labeling Kit (Affymetrix) was used for synthesizing cRNA with oligo(dT) primers starting from 9 µl total RNA in solution
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol of 3' IVT expression Kit.
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner G3000.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Feng,,Cao
Sample_contact_department	Bioscience
Sample_contact_institute	Tokyo University of Agriculture
Sample_contact_address	Sakuragaoka 1-1-1, Setagaya-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	156-8502
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1111nnn/GSM1111640/suppl/GSM1111640_Mouse_GV_XX-1.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1111nnn/GSM1111640/suppl/GSM1111640_Mouse_GV_XX-1.CHP.gz
Sample_series_id	GSE45668
Sample_data_row_count	45101

GSM1111641

GPL1261

GV oocytes, XX, biological rep2

Mouse GV oocytes (XX)

genotype: XX cell type: Oocytes Stage: germinal vesicle (GV)

Gene expression from Mouse GV oocytes

Sample_geo_accession	GSM1111641
Sample_status	Public on Apr 02 2013
Sample_submission_date	Apr 01 2013
Sample_last_update_date	Apr 02 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Ovaries were treated with 0.5 mg/ml each of testicular hyaluronidase (type IV), collagenase (B grade) and egg white lysozyme (all from Sigma) in M2 medium as previously reported. Oocytes with 40–60 µm diameter were collected with a fine glass needle, transferred in a group into a microcentrifuge tube, and stored at −80°C
Sample_growth_protocol_ch1	XX, XY and XO females at 25–29 dpp were injected intraperitoneally each with 5 IU equine chorionic gonadotropin (Sigma, St Louis, MO), and sacrificed 45–47 hours later. Fully grown GV-stage oocytes surrounded by cumulus cells were isolated by puncturing large antral follicles with a pair of 26-gauge needles, and then denuded of cumulus cells by repeated pipetting through a fine glass needle. Only the oocytes with an intact GV and no apparent sign of degeneration were collected and stored at −80°C. Growing oocytes were collected from XX, XY, and XO females at 10 and 17 dpp.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA were isolated from 10 oocytes each from 3 XY and 3 XX females using the RNeasy Micro Kit (Qiagen, Valencia, CA) and extracted in 11 µl RNase-free water. The Two-Cycle Eukaryotic Target Labeling Kit (Affymetrix) was used for synthesizing cRNA with oligo(dT) primers starting from 9 µl total RNA in solution
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol of 3' IVT expression Kit.
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner G3000.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Feng,,Cao
Sample_contact_department	Bioscience
Sample_contact_institute	Tokyo University of Agriculture
Sample_contact_address	Sakuragaoka 1-1-1, Setagaya-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	156-8502
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1111nnn/GSM1111641/suppl/GSM1111641_Mouse_GV_XX-2.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1111nnn/GSM1111641/suppl/GSM1111641_Mouse_GV_XX-2.CHP.gz
Sample_series_id	GSE45668
Sample_data_row_count	45101

GSM1111642

GPL1261

GV oocytes, XX, biological rep3

Mouse GV oocytes (XX)

genotype: XX cell type: Oocytes Stage: germinal vesicle (GV)

Gene expression from Mouse GV oocytes

Sample_geo_accession	GSM1111642
Sample_status	Public on Apr 02 2013
Sample_submission_date	Apr 01 2013
Sample_last_update_date	Apr 02 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Ovaries were treated with 0.5 mg/ml each of testicular hyaluronidase (type IV), collagenase (B grade) and egg white lysozyme (all from Sigma) in M2 medium as previously reported. Oocytes with 40–60 µm diameter were collected with a fine glass needle, transferred in a group into a microcentrifuge tube, and stored at −80°C
Sample_growth_protocol_ch1	XX, XY and XO females at 25–29 dpp were injected intraperitoneally each with 5 IU equine chorionic gonadotropin (Sigma, St Louis, MO), and sacrificed 45–47 hours later. Fully grown GV-stage oocytes surrounded by cumulus cells were isolated by puncturing large antral follicles with a pair of 26-gauge needles, and then denuded of cumulus cells by repeated pipetting through a fine glass needle. Only the oocytes with an intact GV and no apparent sign of degeneration were collected and stored at −80°C. Growing oocytes were collected from XX, XY, and XO females at 10 and 17 dpp.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA were isolated from 10 oocytes each from 3 XY and 3 XX females using the RNeasy Micro Kit (Qiagen, Valencia, CA) and extracted in 11 µl RNase-free water. The Two-Cycle Eukaryotic Target Labeling Kit (Affymetrix) was used for synthesizing cRNA with oligo(dT) primers starting from 9 µl total RNA in solution
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol of 3' IVT expression Kit.
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner G3000.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Feng,,Cao
Sample_contact_department	Bioscience
Sample_contact_institute	Tokyo University of Agriculture
Sample_contact_address	Sakuragaoka 1-1-1, Setagaya-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	156-8502
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1111nnn/GSM1111642/suppl/GSM1111642_Mouse_GV_XX-3.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1111nnn/GSM1111642/suppl/GSM1111642_Mouse_GV_XX-3.CHP.gz
Sample_series_id	GSE45668
Sample_data_row_count	45101

GSM1111643

GPL1261

GV oocytes, XY, biological rep1

Mouse GV oocytes (XY)

genotype: XY cell type: Oocytes Stage: germinal vesicle (GV)

Gene expression from Mouse GV oocytes

Sample_geo_accession	GSM1111643
Sample_status	Public on Apr 02 2013
Sample_submission_date	Apr 01 2013
Sample_last_update_date	Apr 02 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Ovaries were treated with 0.5 mg/ml each of testicular hyaluronidase (type IV), collagenase (B grade) and egg white lysozyme (all from Sigma) in M2 medium as previously reported. Oocytes with 40–60 µm diameter were collected with a fine glass needle, transferred in a group into a microcentrifuge tube, and stored at −80°C
Sample_growth_protocol_ch1	XX, XY and XO females at 25–29 dpp were injected intraperitoneally each with 5 IU equine chorionic gonadotropin (Sigma, St Louis, MO), and sacrificed 45–47 hours later. Fully grown GV-stage oocytes surrounded by cumulus cells were isolated by puncturing large antral follicles with a pair of 26-gauge needles, and then denuded of cumulus cells by repeated pipetting through a fine glass needle. Only the oocytes with an intact GV and no apparent sign of degeneration were collected and stored at −80°C. Growing oocytes were collected from XX, XY, and XO females at 10 and 17 dpp.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA were isolated from 10 oocytes each from 3 XY and 3 XX females using the RNeasy Micro Kit (Qiagen, Valencia, CA) and extracted in 11 µl RNase-free water. The Two-Cycle Eukaryotic Target Labeling Kit (Affymetrix) was used for synthesizing cRNA with oligo(dT) primers starting from 9 µl total RNA in solution
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol of 3' IVT expression Kit.
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner G3000.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Feng,,Cao
Sample_contact_department	Bioscience
Sample_contact_institute	Tokyo University of Agriculture
Sample_contact_address	Sakuragaoka 1-1-1, Setagaya-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	156-8502
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1111nnn/GSM1111643/suppl/GSM1111643_Mouse_GV_XY-1.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1111nnn/GSM1111643/suppl/GSM1111643_Mouse_GV_XY-1.CHP.gz
Sample_series_id	GSE45668
Sample_data_row_count	45101

GSM1111644

GPL1261

GV oocytes, XY, biological rep2

Mouse GV oocytes (XY)

genotype: XY cell type: Oocytes Stage: germinal vesicle (GV)

Gene expression from Mouse GV oocytes

Sample_geo_accession	GSM1111644
Sample_status	Public on Apr 02 2013
Sample_submission_date	Apr 01 2013
Sample_last_update_date	Apr 02 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Ovaries were treated with 0.5 mg/ml each of testicular hyaluronidase (type IV), collagenase (B grade) and egg white lysozyme (all from Sigma) in M2 medium as previously reported. Oocytes with 40–60 µm diameter were collected with a fine glass needle, transferred in a group into a microcentrifuge tube, and stored at −80°C
Sample_growth_protocol_ch1	XX, XY and XO females at 25–29 dpp were injected intraperitoneally each with 5 IU equine chorionic gonadotropin (Sigma, St Louis, MO), and sacrificed 45–47 hours later. Fully grown GV-stage oocytes surrounded by cumulus cells were isolated by puncturing large antral follicles with a pair of 26-gauge needles, and then denuded of cumulus cells by repeated pipetting through a fine glass needle. Only the oocytes with an intact GV and no apparent sign of degeneration were collected and stored at −80°C. Growing oocytes were collected from XX, XY, and XO females at 10 and 17 dpp.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA were isolated from 10 oocytes each from 3 XY and 3 XX females using the RNeasy Micro Kit (Qiagen, Valencia, CA) and extracted in 11 µl RNase-free water. The Two-Cycle Eukaryotic Target Labeling Kit (Affymetrix) was used for synthesizing cRNA with oligo(dT) primers starting from 9 µl total RNA in solution
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol of 3' IVT expression Kit.
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner G3000.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Feng,,Cao
Sample_contact_department	Bioscience
Sample_contact_institute	Tokyo University of Agriculture
Sample_contact_address	Sakuragaoka 1-1-1, Setagaya-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	156-8502
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1111nnn/GSM1111644/suppl/GSM1111644_Mouse_GV_XY-2.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1111nnn/GSM1111644/suppl/GSM1111644_Mouse_GV_XY-2.CHP.gz
Sample_series_id	GSE45668
Sample_data_row_count	45101

GSM1111645

GPL1261

GV oocytes, XY, biological rep3

Mouse GV oocytes (XY)

genotype: XY cell type: Oocytes Stage: germinal vesicle (GV)

Gene expression from Mouse GV oocytes

Sample_geo_accession	GSM1111645
Sample_status	Public on Apr 02 2013
Sample_submission_date	Apr 01 2013
Sample_last_update_date	Apr 02 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Ovaries were treated with 0.5 mg/ml each of testicular hyaluronidase (type IV), collagenase (B grade) and egg white lysozyme (all from Sigma) in M2 medium as previously reported. Oocytes with 40–60 µm diameter were collected with a fine glass needle, transferred in a group into a microcentrifuge tube, and stored at −80°C
Sample_growth_protocol_ch1	XX, XY and XO females at 25–29 dpp were injected intraperitoneally each with 5 IU equine chorionic gonadotropin (Sigma, St Louis, MO), and sacrificed 45–47 hours later. Fully grown GV-stage oocytes surrounded by cumulus cells were isolated by puncturing large antral follicles with a pair of 26-gauge needles, and then denuded of cumulus cells by repeated pipetting through a fine glass needle. Only the oocytes with an intact GV and no apparent sign of degeneration were collected and stored at −80°C. Growing oocytes were collected from XX, XY, and XO females at 10 and 17 dpp.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA were isolated from 10 oocytes each from 3 XY and 3 XX females using the RNeasy Micro Kit (Qiagen, Valencia, CA) and extracted in 11 µl RNase-free water. The Two-Cycle Eukaryotic Target Labeling Kit (Affymetrix) was used for synthesizing cRNA with oligo(dT) primers starting from 9 µl total RNA in solution
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol of 3' IVT expression Kit.
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner G3000.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Feng,,Cao
Sample_contact_department	Bioscience
Sample_contact_institute	Tokyo University of Agriculture
Sample_contact_address	Sakuragaoka 1-1-1, Setagaya-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	156-8502
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1111nnn/GSM1111645/suppl/GSM1111645_Mouse_GV_XY-3.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1111nnn/GSM1111645/suppl/GSM1111645_Mouse_GV_XY-3.CHP.gz
Sample_series_id	GSE45668
Sample_data_row_count	45101

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