Sample_geo_accession | GSM1112078
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Sample_status | Public on Jun 23 2013
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Sample_submission_date | Apr 02 2013
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Sample_last_update_date | Jun 23 2013
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Sample_type | RNA
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Sample_channel_count | 1
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Sample_organism_ch1 | Mus musculus
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Sample_taxid_ch1 | 10090
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Sample_treatment_protocol_ch1 | FDMCs were sparated by using FACS Aria.
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Sample_growth_protocol_ch1 | FDMCs were induced from splenocyte on FDC line.
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Sample_molecule_ch1 | total RNA
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Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
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Sample_label_ch1 | biotin
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Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA.
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Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained under the Affymetrix Gene Chip Cpmmand Console Software (AGCC).
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Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
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Sample_data_processing | The data were analyzed with Affymetrix Expression Console Software (Expression Console) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500 (TGT500).
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Sample_platform_id | GPL1261
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Sample_contact_name | Masaki,,Magari
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Sample_contact_email | magari@cc.okayama-u.ac.jp
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Sample_contact_institute | Okayama University
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Sample_contact_address | 3-1-1, Tsushima-naka, Kita-ku
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Sample_contact_city | Okayama-shi
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Sample_contact_zip/postal_code | 700-8530
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Sample_contact_country | Japan
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Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1112nnn/GSM1112078/suppl/GSM1112078_FDMCs.CEL.gz
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Sample_series_id | GSE45704
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Sample_data_row_count | 45037
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