Result

Search results for the GEO ID: GSE45759

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM1113836

GPL1261

hox001

Hoxb8 expressing immortalized cells

tissue: bone marrow cell type: early hematopoietic progenitor cells genotype: Hoxb8 expressing

Gene expression data from human primary tumors

Sample_geo_accession	GSM1113836
Sample_status	Public on Jun 05 2013
Sample_submission_date	Apr 03 2013
Sample_last_update_date	Jun 05 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Transformed with fusion construct encoding the hormone binding domain of the estrogen receptor and Hoxb8.
Sample_growth_protocol_ch1	In vitro cell culture in RPMI 1640 supplemented with 10% (v/v) fetal calf serum, 50 mM 2-mercaptoethanol and antibiotics (penicillin G (100 IU/ml) and streptomycin sulfate (100 IU/ml)), 1µM -estradiol and 5% FLT3 ligand-containing supernatant.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Standard Trizol extraction of total RNA and purification using RNeasy Mini kit (QIAGEN) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix one-cycle protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 v2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	The data were normalized with Partek Genomics Suite 6.6 and RMA summarized
Sample_platform_id	GPL1261
Sample_contact_name	David,,Finkelstein
Sample_contact_email	david.finkelstein@stjude.org
Sample_contact_phone	9014953931
Sample_contact_department	Computational Biology
Sample_contact_institute	St Jude Children's Research Hospital
Sample_contact_address	332 N. Lauderdale St.
Sample_contact_city	Memphis
Sample_contact_state	TN
Sample_contact_zip/postal_code	38105
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1113nnn/GSM1113836/suppl/GSM1113836_hox001-430v2.CEL.gz
Sample_series_id	GSE45759
Sample_data_row_count	45101

GSM1113837

GPL1261

hox002

Hoxb8 expressing immortalized cells

tissue: bone marrow cell type: early hematopoietic progenitor cells genotype: Hoxb8 expressing

Gene expression data from human primary tumors

Sample_geo_accession	GSM1113837
Sample_status	Public on Jun 05 2013
Sample_submission_date	Apr 03 2013
Sample_last_update_date	Jun 05 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Transformed with fusion construct encoding the hormone binding domain of the estrogen receptor and Hoxb8.
Sample_growth_protocol_ch1	In vitro cell culture in RPMI 1640 supplemented with 10% (v/v) fetal calf serum, 50 mM 2-mercaptoethanol and antibiotics (penicillin G (100 IU/ml) and streptomycin sulfate (100 IU/ml)), 1µM -estradiol and 5% FLT3 ligand-containing supernatant.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Standard Trizol extraction of total RNA and purification using RNeasy Mini kit (QIAGEN) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix one-cycle protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 v2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	The data were normalized with Partek Genomics Suite 6.6 and RMA summarized
Sample_platform_id	GPL1261
Sample_contact_name	David,,Finkelstein
Sample_contact_email	david.finkelstein@stjude.org
Sample_contact_phone	9014953931
Sample_contact_department	Computational Biology
Sample_contact_institute	St Jude Children's Research Hospital
Sample_contact_address	332 N. Lauderdale St.
Sample_contact_city	Memphis
Sample_contact_state	TN
Sample_contact_zip/postal_code	38105
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1113nnn/GSM1113837/suppl/GSM1113837_hox002-430v2.CEL.gz
Sample_series_id	GSE45759
Sample_data_row_count	45101

GSM1113838

GPL1261

hox011

Hoxb8 expressing immortalized cells

tissue: bone marrow cell type: early hematopoietic progenitor cells genotype: Hoxb8 expressing

Gene expression data from human primary tumors

Sample_geo_accession	GSM1113838
Sample_status	Public on Jun 05 2013
Sample_submission_date	Apr 03 2013
Sample_last_update_date	Jun 05 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Transformed with fusion construct encoding the hormone binding domain of the estrogen receptor and Hoxb8.
Sample_growth_protocol_ch1	In vitro cell culture in RPMI 1640 supplemented with 10% (v/v) fetal calf serum, 50 mM 2-mercaptoethanol and antibiotics (penicillin G (100 IU/ml) and streptomycin sulfate (100 IU/ml)), 1µM -estradiol and 5% FLT3 ligand-containing supernatant.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Standard Trizol extraction of total RNA and purification using RNeasy Mini kit (QIAGEN) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix one-cycle protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 v2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	The data were normalized with Partek Genomics Suite 6.6 and RMA summarized
Sample_platform_id	GPL1261
Sample_contact_name	David,,Finkelstein
Sample_contact_email	david.finkelstein@stjude.org
Sample_contact_phone	9014953931
Sample_contact_department	Computational Biology
Sample_contact_institute	St Jude Children's Research Hospital
Sample_contact_address	332 N. Lauderdale St.
Sample_contact_city	Memphis
Sample_contact_state	TN
Sample_contact_zip/postal_code	38105
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1113nnn/GSM1113838/suppl/GSM1113838_hox011-430v2.CEL.gz
Sample_series_id	GSE45759
Sample_data_row_count	45101

GSM1113839

GPL1261

hox012

Hoxb8 expressing immortalized cells

tissue: bone marrow cell type: early hematopoietic progenitor cells genotype: Hoxb8 expressing

Gene expression data from human primary tumors

Sample_geo_accession	GSM1113839
Sample_status	Public on Jun 05 2013
Sample_submission_date	Apr 03 2013
Sample_last_update_date	Jun 05 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Transformed with fusion construct encoding the hormone binding domain of the estrogen receptor and Hoxb8.
Sample_growth_protocol_ch1	In vitro cell culture in RPMI 1640 supplemented with 10% (v/v) fetal calf serum, 50 mM 2-mercaptoethanol and antibiotics (penicillin G (100 IU/ml) and streptomycin sulfate (100 IU/ml)), 1µM -estradiol and 5% FLT3 ligand-containing supernatant.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Standard Trizol extraction of total RNA and purification using RNeasy Mini kit (QIAGEN) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix one-cycle protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 v2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	The data were normalized with Partek Genomics Suite 6.6 and RMA summarized
Sample_platform_id	GPL1261
Sample_contact_name	David,,Finkelstein
Sample_contact_email	david.finkelstein@stjude.org
Sample_contact_phone	9014953931
Sample_contact_department	Computational Biology
Sample_contact_institute	St Jude Children's Research Hospital
Sample_contact_address	332 N. Lauderdale St.
Sample_contact_city	Memphis
Sample_contact_state	TN
Sample_contact_zip/postal_code	38105
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1113nnn/GSM1113839/suppl/GSM1113839_hox012-430v2.CEL.gz
Sample_series_id	GSE45759
Sample_data_row_count	45101

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