Search results for the GEO ID: GSE46143  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1124967 | GPL570 |
|
GC B cell_pSG5_T73
|
Germinal centre (GC) B cells isolated from tonsils via CD10 enrichment, transfected with empty vector
|
patient: T73
cell type: CD10+ positive, Germinal Centre (GC) B cells
genotype/variation: control
|
Total RNA from FACS-sorted GC B cells
|
| Sample_geo_accession | GSM1124967
| | Sample_status | Public on Apr 19 2013
| | Sample_submission_date | Apr 17 2013
| | Sample_last_update_date | Apr 19 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Enriched CD10+ positive cells were transfected and FACS-sorted
| | Sample_growth_protocol_ch1 | Germinal centre (GC) B cells were isolated from tonsils via CD10 enrichment
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Isolation of total RNA from sorted cells was performed using the RNeasy® Micro Kit from Qiagen (Crawley, UK) according to the manufacturer’s instructions. RNA from transfected and FACS-sorted GC B cells was amplified with Standard ExpressArt® C&E mRNA amplification kit (Amptech).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 25 ug total RNA
| | Sample_hyb_protocol | 10ug of fragmented cRNA was hybridized to HG-U133 Plus 2.0 microarrays
| | Sample_scan_protocol | Affymetrix Standard protocol Affymetrix Standard protocol
| | Sample_data_processing | Probe level quantile normalisation and robust multi-array analysis on the raw CEL files were performed using the affy package of the Bioconductor project using default parameters
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenbin,,Wei
| | Sample_contact_email | w.wei@bham.ac.uk
| | Sample_contact_phone | +44-121-4143293
| | Sample_contact_laboratory | Bioinformatics
| | Sample_contact_department | School of Cancer Sciences
| | Sample_contact_institute | University of Birmingham
| | Sample_contact_address | Vincent Drive
| | Sample_contact_city | Edgbaston
| | Sample_contact_state | West Midlands
| | Sample_contact_zip/postal_code | B15 2TT
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1124nnn/GSM1124967/suppl/GSM1124967_T73_PSG_RNA_060509.CEL.gz
| | Sample_series_id | GSE46143
| | Sample_data_row_count | 54675
| | |
|
GSM1124969 | GPL570 |
|
GC B cell_pSG5_T78
|
Germinal centre (GC) B cells isolated from tonsils via CD10 enrichment, transfected with empty vector
|
patient: T78
cell type: CD10+ positive, Germinal Centre (GC) B cells
genotype/variation: control
|
Total RNA from FACS-sorted GC B cells
|
| Sample_geo_accession | GSM1124969
| | Sample_status | Public on Apr 19 2013
| | Sample_submission_date | Apr 17 2013
| | Sample_last_update_date | Apr 19 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Enriched CD10+ positive cells were transfected and FACS-sorted
| | Sample_growth_protocol_ch1 | Germinal centre (GC) B cells were isolated from tonsils via CD10 enrichment
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Isolation of total RNA from sorted cells was performed using the RNeasy® Micro Kit from Qiagen (Crawley, UK) according to the manufacturer’s instructions. RNA from transfected and FACS-sorted GC B cells was amplified with Standard ExpressArt® C&E mRNA amplification kit (Amptech).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 25 ug total RNA
| | Sample_hyb_protocol | 10ug of fragmented cRNA was hybridized to HG-U133 Plus 2.0 microarrays
| | Sample_scan_protocol | Affymetrix Standard protocol Affymetrix Standard protocol
| | Sample_data_processing | Probe level quantile normalisation and robust multi-array analysis on the raw CEL files were performed using the affy package of the Bioconductor project using default parameters
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenbin,,Wei
| | Sample_contact_email | w.wei@bham.ac.uk
| | Sample_contact_phone | +44-121-4143293
| | Sample_contact_laboratory | Bioinformatics
| | Sample_contact_department | School of Cancer Sciences
| | Sample_contact_institute | University of Birmingham
| | Sample_contact_address | Vincent Drive
| | Sample_contact_city | Edgbaston
| | Sample_contact_state | West Midlands
| | Sample_contact_zip/postal_code | B15 2TT
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1124nnn/GSM1124969/suppl/GSM1124969_T78_PSG_RNA_110509.CEL.gz
| | Sample_series_id | GSE46143
| | Sample_data_row_count | 54675
| | |
|
GSM1124970 | GPL570 |
|
GC B cell_LMP2A_T73
|
Germinal centre (GC) B cells isolated from tonsils via CD10 enrichment, transfected with LMP2A expression vector
|
patient: T73
cell type: CD10+ positive, Germinal Centre (GC) B cells
genotype/variation: LMP2A-transfected
|
Total RNA from FACS-sorted GC B cells
|
| Sample_geo_accession | GSM1124970
| | Sample_status | Public on Apr 19 2013
| | Sample_submission_date | Apr 17 2013
| | Sample_last_update_date | Apr 19 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Enriched CD10+ positive cells were transfected and FACS-sorted
| | Sample_growth_protocol_ch1 | Germinal centre (GC) B cells were isolated from tonsils via CD10 enrichment
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Isolation of total RNA from sorted cells was performed using the RNeasy® Micro Kit from Qiagen (Crawley, UK) according to the manufacturer’s instructions. RNA from transfected and FACS-sorted GC B cells was amplified with Standard ExpressArt® C&E mRNA amplification kit (Amptech).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 25 ug total RNA
| | Sample_hyb_protocol | 10ug of fragmented cRNA was hybridized to HG-U133 Plus 2.0 microarrays
| | Sample_scan_protocol | Affymetrix Standard protocol Affymetrix Standard protocol
| | Sample_data_processing | Probe level quantile normalisation and robust multi-array analysis on the raw CEL files were performed using the affy package of the Bioconductor project using default parameters
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenbin,,Wei
| | Sample_contact_email | w.wei@bham.ac.uk
| | Sample_contact_phone | +44-121-4143293
| | Sample_contact_laboratory | Bioinformatics
| | Sample_contact_department | School of Cancer Sciences
| | Sample_contact_institute | University of Birmingham
| | Sample_contact_address | Vincent Drive
| | Sample_contact_city | Edgbaston
| | Sample_contact_state | West Midlands
| | Sample_contact_zip/postal_code | B15 2TT
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1124nnn/GSM1124970/suppl/GSM1124970_T73_LMP2_RNA_060509.CEL.gz
| | Sample_series_id | GSE46143
| | Sample_data_row_count | 54675
| | |
|
GSM1124971 | GPL570 |
|
GC B cell_LMP2A_T75
|
Germinal centre (GC) B cells isolated from tonsils via CD10 enrichment, transfected with LMP2A expression vector
|
patient: T75
cell type: CD10+ positive, Germinal Centre (GC) B cells
genotype/variation: LMP2A-transfected
|
Total RNA from FACS-sorted GC B cells
|
| Sample_geo_accession | GSM1124971
| | Sample_status | Public on Apr 19 2013
| | Sample_submission_date | Apr 17 2013
| | Sample_last_update_date | Apr 19 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Enriched CD10+ positive cells were transfected and FACS-sorted
| | Sample_growth_protocol_ch1 | Germinal centre (GC) B cells were isolated from tonsils via CD10 enrichment
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Isolation of total RNA from sorted cells was performed using the RNeasy® Micro Kit from Qiagen (Crawley, UK) according to the manufacturer’s instructions. RNA from transfected and FACS-sorted GC B cells was amplified with Standard ExpressArt® C&E mRNA amplification kit (Amptech).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 25 ug total RNA
| | Sample_hyb_protocol | 10ug of fragmented cRNA was hybridized to HG-U133 Plus 2.0 microarrays
| | Sample_scan_protocol | Affymetrix Standard protocol Affymetrix Standard protocol
| | Sample_data_processing | Probe level quantile normalisation and robust multi-array analysis on the raw CEL files were performed using the affy package of the Bioconductor project using default parameters
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenbin,,Wei
| | Sample_contact_email | w.wei@bham.ac.uk
| | Sample_contact_phone | +44-121-4143293
| | Sample_contact_laboratory | Bioinformatics
| | Sample_contact_department | School of Cancer Sciences
| | Sample_contact_institute | University of Birmingham
| | Sample_contact_address | Vincent Drive
| | Sample_contact_city | Edgbaston
| | Sample_contact_state | West Midlands
| | Sample_contact_zip/postal_code | B15 2TT
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1124nnn/GSM1124971/suppl/GSM1124971_T75_LMP2_RNA_060509.CEL.gz
| | Sample_series_id | GSE46143
| | Sample_data_row_count | 54675
| | |
|
GSM1124972 | GPL570 |
|
GC B cell_LMP2A_T78
|
Germinal centre (GC) B cells isolated from tonsils via CD10 enrichment, transfected with LMP2A expression vector
|
patient: T78
cell type: CD10+ positive, Germinal Centre (GC) B cells
genotype/variation: LMP2A-transfected
|
Total RNA from FACS-sorted GC B cells
|
| Sample_geo_accession | GSM1124972
| | Sample_status | Public on Apr 19 2013
| | Sample_submission_date | Apr 17 2013
| | Sample_last_update_date | Apr 19 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Enriched CD10+ positive cells were transfected and FACS-sorted
| | Sample_growth_protocol_ch1 | Germinal centre (GC) B cells were isolated from tonsils via CD10 enrichment
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Isolation of total RNA from sorted cells was performed using the RNeasy® Micro Kit from Qiagen (Crawley, UK) according to the manufacturer’s instructions. RNA from transfected and FACS-sorted GC B cells was amplified with Standard ExpressArt® C&E mRNA amplification kit (Amptech).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 25 ug total RNA
| | Sample_hyb_protocol | 10ug of fragmented cRNA was hybridized to HG-U133 Plus 2.0 microarrays
| | Sample_scan_protocol | Affymetrix Standard protocol Affymetrix Standard protocol
| | Sample_data_processing | Probe level quantile normalisation and robust multi-array analysis on the raw CEL files were performed using the affy package of the Bioconductor project using default parameters
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenbin,,Wei
| | Sample_contact_email | w.wei@bham.ac.uk
| | Sample_contact_phone | +44-121-4143293
| | Sample_contact_laboratory | Bioinformatics
| | Sample_contact_department | School of Cancer Sciences
| | Sample_contact_institute | University of Birmingham
| | Sample_contact_address | Vincent Drive
| | Sample_contact_city | Edgbaston
| | Sample_contact_state | West Midlands
| | Sample_contact_zip/postal_code | B15 2TT
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1124nnn/GSM1124972/suppl/GSM1124972_T78_LMP2_RNA_110509.CEL.gz
| | Sample_series_id | GSE46143
| | Sample_data_row_count | 54675
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
