Result

Search results for the GEO ID: GSE46218

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GSM ID

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Title

Source name

Description

Characteristics

GSM1126521

GPL570

uncastrasion-75

uncastrasion-75 LNCAP cell Orhotopic xenograft tumor of prostate cancer

cell type: LNCAP host strain: Balb/c nude tissue: mouse prostate intervention: uncastrasion

Gene expression data from uncastrasion tumor-75

Sample_geo_accession	GSM1126521
Sample_status	Public on Apr 20 2013
Sample_submission_date	Apr 19 2013
Sample_last_update_date	Apr 20 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	half of the mice were castrated when tumors reached 10mm and the tumors were allowed to growth back and later transplanted orthotopically into the prostate ventral lobe of intact
Sample_growth_protocol_ch1	Ten male athymic Balb/c nude mice (Beijing HFK Bio-Technology Co. Ltd.) were injected subcutaneouslly with 2×106 LNCaP cells suspended in 0.1 mL of Matrigel (BD Biosciences), half of the mice were castrated when tumors reached 10mm and the tumors were allowed to growth back and later transplanted orthotopically into the prostate ventral lobe of intact or castrated Balb/c mice. The later procedure was repeated at least twice before tumors were dissected and snap frozen for further analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200ng total RNA (3' IVT Express Kit User Manual, 2008, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Wheat Genome Array in the Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the affymetrix scanner 3000 7G.The scanned images were first assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of AGCC.
Sample_data_processing	The data were normalized using Robust Multi-array Average(RMA) .
Sample_platform_id	GPL570
Sample_contact_name	ning,,jiang
Sample_contact_email	jnapple@gmail.com
Sample_contact_phone	086+022-88329296
Sample_contact_department	urology institute
Sample_contact_institute	tianjin medical university
Sample_contact_address	hexi district pingjiang road 23
Sample_contact_city	tianjin
Sample_contact_zip/postal_code	300211
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126521/suppl/GSM1126521_101224-10352B_75_HG-U133_Plus_2_.CEL.gz
Sample_series_id	GSE46218
Sample_data_row_count	54675

GSM1126522

GPL570

uncastrasion-51P

uncastrasion-51P LNCAP cell Orhotopic xenograft tumor of prostate cancer

cell type: LNCAP host strain: Balb/c nude tissue: mouse prostate intervention: uncastrasion

Gene expression data from uncastrasion tumor-51P

Sample_geo_accession	GSM1126522
Sample_status	Public on Apr 20 2013
Sample_submission_date	Apr 19 2013
Sample_last_update_date	Apr 20 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	half of the mice were castrated when tumors reached 10mm and the tumors were allowed to growth back and later transplanted orthotopically into the prostate ventral lobe of intact
Sample_growth_protocol_ch1	Ten male athymic Balb/c nude mice (Beijing HFK Bio-Technology Co. Ltd.) were injected subcutaneouslly with 2×106 LNCaP cells suspended in 0.1 mL of Matrigel (BD Biosciences), half of the mice were castrated when tumors reached 10mm and the tumors were allowed to growth back and later transplanted orthotopically into the prostate ventral lobe of intact or castrated Balb/c mice. The later procedure was repeated at least twice before tumors were dissected and snap frozen for further analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200ng total RNA (3' IVT Express Kit User Manual, 2008, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Wheat Genome Array in the Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the affymetrix scanner 3000 7G.The scanned images were first assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of AGCC.
Sample_data_processing	The data were normalized using Robust Multi-array Average(RMA) .
Sample_platform_id	GPL570
Sample_contact_name	ning,,jiang
Sample_contact_email	jnapple@gmail.com
Sample_contact_phone	086+022-88329296
Sample_contact_department	urology institute
Sample_contact_institute	tianjin medical university
Sample_contact_address	hexi district pingjiang road 23
Sample_contact_city	tianjin
Sample_contact_zip/postal_code	300211
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126522/suppl/GSM1126522_100114-HG-U133_Plus_2-09930A_51P.CEL.gz
Sample_series_id	GSE46218
Sample_data_row_count	54675

GSM1126523

GPL570

uncastrasion-65P

uncastrasion-65P LNCAP cell Orhotopic xenograft tumor of prostate cancer

cell type: LNCAP host strain: Balb/c nude tissue: mouse prostate intervention: uncastrasion

Gene expression data from uncastrasion tumor-65P

Sample_geo_accession	GSM1126523
Sample_status	Public on Apr 20 2013
Sample_submission_date	Apr 19 2013
Sample_last_update_date	Apr 20 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	half of the mice were castrated when tumors reached 10mm and the tumors were allowed to growth back and later transplanted orthotopically into the prostate ventral lobe of intact
Sample_growth_protocol_ch1	Ten male athymic Balb/c nude mice (Beijing HFK Bio-Technology Co. Ltd.) were injected subcutaneouslly with 2×106 LNCaP cells suspended in 0.1 mL of Matrigel (BD Biosciences), half of the mice were castrated when tumors reached 10mm and the tumors were allowed to growth back and later transplanted orthotopically into the prostate ventral lobe of intact or castrated Balb/c mice. The later procedure was repeated at least twice before tumors were dissected and snap frozen for further analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200ng total RNA (3' IVT Express Kit User Manual, 2008, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Wheat Genome Array in the Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the affymetrix scanner 3000 7G.The scanned images were first assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of AGCC.
Sample_data_processing	The data were normalized using Robust Multi-array Average(RMA) .
Sample_platform_id	GPL570
Sample_contact_name	ning,,jiang
Sample_contact_email	jnapple@gmail.com
Sample_contact_phone	086+022-88329296
Sample_contact_department	urology institute
Sample_contact_institute	tianjin medical university
Sample_contact_address	hexi district pingjiang road 23
Sample_contact_city	tianjin
Sample_contact_zip/postal_code	300211
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126523/suppl/GSM1126523_100316-HG_U133_Plus_2-09930B_65P.CEL.gz
Sample_series_id	GSE46218
Sample_data_row_count	54675

GSM1126524

GPL570

uncastrasion-66P

uncastrasion-66P LNCAP cell Orhotopic xenograft tumor of prostate cancer

cell type: LNCAP host strain: Balb/c nude tissue: mouse prostate intervention: uncastrasion

Gene expression data from uncastrasion tumor-66P

Sample_geo_accession	GSM1126524
Sample_status	Public on Apr 20 2013
Sample_submission_date	Apr 19 2013
Sample_last_update_date	Apr 20 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	half of the mice were castrated when tumors reached 10mm and the tumors were allowed to growth back and later transplanted orthotopically into the prostate ventral lobe of intact
Sample_growth_protocol_ch1	Ten male athymic Balb/c nude mice (Beijing HFK Bio-Technology Co. Ltd.) were injected subcutaneouslly with 2×106 LNCaP cells suspended in 0.1 mL of Matrigel (BD Biosciences), half of the mice were castrated when tumors reached 10mm and the tumors were allowed to growth back and later transplanted orthotopically into the prostate ventral lobe of intact or castrated Balb/c mice. The later procedure was repeated at least twice before tumors were dissected and snap frozen for further analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200ng total RNA (3' IVT Express Kit User Manual, 2008, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Wheat Genome Array in the Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the affymetrix scanner 3000 7G.The scanned images were first assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of AGCC.
Sample_data_processing	The data were normalized using Robust Multi-array Average(RMA) .
Sample_platform_id	GPL570
Sample_contact_name	ning,,jiang
Sample_contact_email	jnapple@gmail.com
Sample_contact_phone	086+022-88329296
Sample_contact_department	urology institute
Sample_contact_institute	tianjin medical university
Sample_contact_address	hexi district pingjiang road 23
Sample_contact_city	tianjin
Sample_contact_zip/postal_code	300211
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126524/suppl/GSM1126524_100316-HG_U133_Plus_2-09930B_66P.CEL.gz
Sample_series_id	GSE46218
Sample_data_row_count	54675

GSM1126525

GPL570

castration-90

castration-90 LNCAP cell Orhotopic xenograft tumor of prostate cancer

cell type: LNCAP host strain: Balb/c nude tissue: mouse prostate intervention: castration

Gene expression data from castrasion tumor-90

Sample_geo_accession	GSM1126525
Sample_status	Public on Apr 20 2013
Sample_submission_date	Apr 19 2013
Sample_last_update_date	Apr 20 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	half of the mice were castrated when tumors reached 10mm and the tumors were allowed to growth back and later transplanted orthotopically into the prostate ventral lobe of intact
Sample_growth_protocol_ch1	Ten male athymic Balb/c nude mice (Beijing HFK Bio-Technology Co. Ltd.) were injected subcutaneouslly with 2×106 LNCaP cells suspended in 0.1 mL of Matrigel (BD Biosciences), half of the mice were castrated when tumors reached 10mm and the tumors were allowed to growth back and later transplanted orthotopically into the prostate ventral lobe of intact or castrated Balb/c mice. The later procedure was repeated at least twice before tumors were dissected and snap frozen for further analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200ng total RNA (3' IVT Express Kit User Manual, 2008, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Wheat Genome Array in the Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the affymetrix scanner 3000 7G.The scanned images were first assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of AGCC.
Sample_data_processing	The data were normalized using Robust Multi-array Average(RMA) .
Sample_platform_id	GPL570
Sample_contact_name	ning,,jiang
Sample_contact_email	jnapple@gmail.com
Sample_contact_phone	086+022-88329296
Sample_contact_department	urology institute
Sample_contact_institute	tianjin medical university
Sample_contact_address	hexi district pingjiang road 23
Sample_contact_city	tianjin
Sample_contact_zip/postal_code	300211
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126525/suppl/GSM1126525_101224-10352B_90_HG-U133_Plus_2_.CEL.gz
Sample_series_id	GSE46218
Sample_data_row_count	54675

GSM1126526

GPL570

castration-91

castration-91 LNCAP cell Orhotopic xenograft tumor of prostate cancer

cell type: LNCAP host strain: Balb/c nude tissue: mouse prostate intervention: castration

Gene expression data from castrasion tumor-91

Sample_geo_accession	GSM1126526
Sample_status	Public on Apr 20 2013
Sample_submission_date	Apr 19 2013
Sample_last_update_date	Apr 20 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	half of the mice were castrated when tumors reached 10mm and the tumors were allowed to growth back and later transplanted orthotopically into the prostate ventral lobe of intact
Sample_growth_protocol_ch1	Ten male athymic Balb/c nude mice (Beijing HFK Bio-Technology Co. Ltd.) were injected subcutaneouslly with 2×106 LNCaP cells suspended in 0.1 mL of Matrigel (BD Biosciences), half of the mice were castrated when tumors reached 10mm and the tumors were allowed to growth back and later transplanted orthotopically into the prostate ventral lobe of intact or castrated Balb/c mice. The later procedure was repeated at least twice before tumors were dissected and snap frozen for further analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200ng total RNA (3' IVT Express Kit User Manual, 2008, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Wheat Genome Array in the Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the affymetrix scanner 3000 7G.The scanned images were first assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of AGCC.
Sample_data_processing	The data were normalized using Robust Multi-array Average(RMA) .
Sample_platform_id	GPL570
Sample_contact_name	ning,,jiang
Sample_contact_email	jnapple@gmail.com
Sample_contact_phone	086+022-88329296
Sample_contact_department	urology institute
Sample_contact_institute	tianjin medical university
Sample_contact_address	hexi district pingjiang road 23
Sample_contact_city	tianjin
Sample_contact_zip/postal_code	300211
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126526/suppl/GSM1126526_101224-10352B_91_HG-U133_Plus_2_.CEL.gz
Sample_series_id	GSE46218
Sample_data_row_count	54675

GSM1126527

GPL570

castration-93

castration-93 LNCAP cell Orhotopic xenograft tumor of prostate cancer

cell type: LNCAP host strain: Balb/c nude tissue: mouse prostate intervention: castration

Gene expression data from uncastrasion tumor-93

Sample_geo_accession	GSM1126527
Sample_status	Public on Apr 20 2013
Sample_submission_date	Apr 19 2013
Sample_last_update_date	Apr 20 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	half of the mice were castrated when tumors reached 10mm and the tumors were allowed to growth back and later transplanted orthotopically into the prostate ventral lobe of intact
Sample_growth_protocol_ch1	Ten male athymic Balb/c nude mice (Beijing HFK Bio-Technology Co. Ltd.) were injected subcutaneouslly with 2×106 LNCaP cells suspended in 0.1 mL of Matrigel (BD Biosciences), half of the mice were castrated when tumors reached 10mm and the tumors were allowed to growth back and later transplanted orthotopically into the prostate ventral lobe of intact or castrated Balb/c mice. The later procedure was repeated at least twice before tumors were dissected and snap frozen for further analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200ng total RNA (3' IVT Express Kit User Manual, 2008, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Wheat Genome Array in the Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the affymetrix scanner 3000 7G.The scanned images were first assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of AGCC.
Sample_data_processing	The data were normalized using Robust Multi-array Average(RMA) .
Sample_platform_id	GPL570
Sample_contact_name	ning,,jiang
Sample_contact_email	jnapple@gmail.com
Sample_contact_phone	086+022-88329296
Sample_contact_department	urology institute
Sample_contact_institute	tianjin medical university
Sample_contact_address	hexi district pingjiang road 23
Sample_contact_city	tianjin
Sample_contact_zip/postal_code	300211
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126527/suppl/GSM1126527_101224-10352B_93_HG-U133_Plus_2_.CEL.gz
Sample_series_id	GSE46218
Sample_data_row_count	54675

GSM1126528

GPL570

castration-57P

castration-57P LNCAP cell Orhotopic xenograft tumor of prostate cancer

cell type: LNCAP host strain: Balb/c nude tissue: mouse prostate intervention: castration

Gene expression data from castrasion tumor-57P

Sample_geo_accession	GSM1126528
Sample_status	Public on Apr 20 2013
Sample_submission_date	Apr 19 2013
Sample_last_update_date	Apr 20 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	half of the mice were castrated when tumors reached 10mm and the tumors were allowed to growth back and later transplanted orthotopically into the prostate ventral lobe of intact
Sample_growth_protocol_ch1	Ten male athymic Balb/c nude mice (Beijing HFK Bio-Technology Co. Ltd.) were injected subcutaneouslly with 2×106 LNCaP cells suspended in 0.1 mL of Matrigel (BD Biosciences), half of the mice were castrated when tumors reached 10mm and the tumors were allowed to growth back and later transplanted orthotopically into the prostate ventral lobe of intact or castrated Balb/c mice. The later procedure was repeated at least twice before tumors were dissected and snap frozen for further analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200ng total RNA (3' IVT Express Kit User Manual, 2008, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Wheat Genome Array in the Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the affymetrix scanner 3000 7G.The scanned images were first assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of AGCC.
Sample_data_processing	The data were normalized using Robust Multi-array Average(RMA) .
Sample_platform_id	GPL570
Sample_contact_name	ning,,jiang
Sample_contact_email	jnapple@gmail.com
Sample_contact_phone	086+022-88329296
Sample_contact_department	urology institute
Sample_contact_institute	tianjin medical university
Sample_contact_address	hexi district pingjiang road 23
Sample_contact_city	tianjin
Sample_contact_zip/postal_code	300211
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126528/suppl/GSM1126528_100119-HG-U133_Plus_2-09930A_57P.CEL.gz
Sample_series_id	GSE46218
Sample_data_row_count	54675

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