Search results for the GEO ID: GSE46242  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1126962 | GPL1261 |
|
Control EV rep1
|
Control wild-type Th1 T cells
|
strain: C57BL/6
cell type: Th1 T cells
genotype/variation: wild-type
treatment: untreated (Control)
|
Gene expression data from control wild-type Th1 T cells
Ctrl EV 01
|
| Sample_geo_accession | GSM1126962
| | Sample_status | Public on Apr 21 2013
| | Sample_submission_date | Apr 20 2013
| | Sample_last_update_date | Apr 21 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | CAR Tg x Egr2flox/flox Th1 T cells were infected with an empty (EV) or a Cre-expressing adenovirus, Egr2 deletion was confirmed by immunoblot. The T cells were then left untreated (Control) or anergized (Anergic) by immobilized anti-CD3 for 16 hours, and microarray was conducted after 1 day of rest in culture medium.
| | Sample_growth_protocol_ch1 | OVA-specific Th1 cell clones were generated from CAR Tg x Egr2flox/flox mice.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was purified using RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The RNAs were labeled with biotin and fragmented using GeneChip 3’ IVT Express Kit according to Affymetrix protocol.
| | Sample_hyb_protocol | The fragmented cRNA (12 µg) was then hybridized to Affymetrix mouse genome 430 2.0 expression arrays for 16 h at 45°C with 60 rpm in Affymetrix hybridization oven 640.
| | Sample_scan_protocol | The arrays were scanned by Affymetrix Gene Chip Scanner 3000 7G and CEL.
| | Sample_data_processing | Intensity files were generated by Gene Chip Operating Software v. 1.4 (MicroArray Suite 5.0)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yan,,Zheng
| | Sample_contact_email | zheng418@hotmail.com
| | Sample_contact_institute | University of Chicago
| | Sample_contact_address | 929 E. 57th St.
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126962/suppl/GSM1126962_Ctrl_EV_01.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126962/suppl/GSM1126962_Ctrl_EV_01.CHP.gz
| | Sample_series_id | GSE46242
| | Sample_series_id | GSE46243
| | Sample_data_row_count | 45101
| | |
|
GSM1126963 | GPL1261 |
|
Control Cre rep1
|
Control Egr2-deleted Th1 T cells
|
strain: C57BL/6
cell type: Th1 T cells
genotype/variation: Egr2-deleted
treatment: untreated (Control)
|
Gene expressoin data from control Egr2-deleted Th1 T cells
Ctrl Cre 01
|
| Sample_geo_accession | GSM1126963
| | Sample_status | Public on Apr 21 2013
| | Sample_submission_date | Apr 20 2013
| | Sample_last_update_date | Apr 21 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | CAR Tg x Egr2flox/flox Th1 T cells were infected with an empty (EV) or a Cre-expressing adenovirus, Egr2 deletion was confirmed by immunoblot. The T cells were then left untreated (Control) or anergized (Anergic) by immobilized anti-CD3 for 16 hours, and microarray was conducted after 1 day of rest in culture medium.
| | Sample_growth_protocol_ch1 | OVA-specific Th1 cell clones were generated from CAR Tg x Egr2flox/flox mice.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was purified using RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The RNAs were labeled with biotin and fragmented using GeneChip 3’ IVT Express Kit according to Affymetrix protocol.
| | Sample_hyb_protocol | The fragmented cRNA (12 µg) was then hybridized to Affymetrix mouse genome 430 2.0 expression arrays for 16 h at 45°C with 60 rpm in Affymetrix hybridization oven 640.
| | Sample_scan_protocol | The arrays were scanned by Affymetrix Gene Chip Scanner 3000 7G and CEL.
| | Sample_data_processing | Intensity files were generated by Gene Chip Operating Software v. 1.4 (MicroArray Suite 5.0)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yan,,Zheng
| | Sample_contact_email | zheng418@hotmail.com
| | Sample_contact_institute | University of Chicago
| | Sample_contact_address | 929 E. 57th St.
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126963/suppl/GSM1126963_Ctrl_Cre_01.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126963/suppl/GSM1126963_Ctrl_Cre_01.CHP.gz
| | Sample_series_id | GSE46242
| | Sample_series_id | GSE46243
| | Sample_data_row_count | 45101
| | |
|
GSM1126964 | GPL1261 |
|
Anergic EV rep1
|
Anergic wild-type Th1 T cells
|
strain: C57BL/6
cell type: Th1 T cells
genotype/variation: wild-type
treatment: immobilized anti-CD3
|
Gene expression data from anergic wild-type Th1 T cells
An EV 01
|
| Sample_geo_accession | GSM1126964
| | Sample_status | Public on Apr 21 2013
| | Sample_submission_date | Apr 20 2013
| | Sample_last_update_date | Apr 21 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | CAR Tg x Egr2flox/flox Th1 T cells were infected with an empty (EV) or a Cre-expressing adenovirus, Egr2 deletion was confirmed by immunoblot. The T cells were then left untreated (Control) or anergized (Anergic) by immobilized anti-CD3 for 16 hours, and microarray was conducted after 1 day of rest in culture medium.
| | Sample_growth_protocol_ch1 | OVA-specific Th1 cell clones were generated from CAR Tg x Egr2flox/flox mice.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was purified using RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The RNAs were labeled with biotin and fragmented using GeneChip 3’ IVT Express Kit according to Affymetrix protocol.
| | Sample_hyb_protocol | The fragmented cRNA (12 µg) was then hybridized to Affymetrix mouse genome 430 2.0 expression arrays for 16 h at 45°C with 60 rpm in Affymetrix hybridization oven 640.
| | Sample_scan_protocol | The arrays were scanned by Affymetrix Gene Chip Scanner 3000 7G and CEL.
| | Sample_data_processing | Intensity files were generated by Gene Chip Operating Software v. 1.4 (MicroArray Suite 5.0)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yan,,Zheng
| | Sample_contact_email | zheng418@hotmail.com
| | Sample_contact_institute | University of Chicago
| | Sample_contact_address | 929 E. 57th St.
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126964/suppl/GSM1126964_An_EV_01.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126964/suppl/GSM1126964_An_EV_01.CHP.gz
| | Sample_series_id | GSE46242
| | Sample_series_id | GSE46243
| | Sample_data_row_count | 45101
| | |
|
GSM1126965 | GPL1261 |
|
Anergic Cre rep1
|
Anergic Egr2-deleted Th1 T cells
|
strain: C57BL/6
cell type: Th1 T cells
genotype/variation: Egr2-deleted
treatment: immobilized anti-CD3
|
Gene expression data from anergic Egr2-deleted Th1 T cells
An Cre 01
|
| Sample_geo_accession | GSM1126965
| | Sample_status | Public on Apr 21 2013
| | Sample_submission_date | Apr 20 2013
| | Sample_last_update_date | Apr 21 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | CAR Tg x Egr2flox/flox Th1 T cells were infected with an empty (EV) or a Cre-expressing adenovirus, Egr2 deletion was confirmed by immunoblot. The T cells were then left untreated (Control) or anergized (Anergic) by immobilized anti-CD3 for 16 hours, and microarray was conducted after 1 day of rest in culture medium.
| | Sample_growth_protocol_ch1 | OVA-specific Th1 cell clones were generated from CAR Tg x Egr2flox/flox mice.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was purified using RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The RNAs were labeled with biotin and fragmented using GeneChip 3’ IVT Express Kit according to Affymetrix protocol.
| | Sample_hyb_protocol | The fragmented cRNA (12 µg) was then hybridized to Affymetrix mouse genome 430 2.0 expression arrays for 16 h at 45°C with 60 rpm in Affymetrix hybridization oven 640.
| | Sample_scan_protocol | The arrays were scanned by Affymetrix Gene Chip Scanner 3000 7G and CEL.
| | Sample_data_processing | Intensity files were generated by Gene Chip Operating Software v. 1.4 (MicroArray Suite 5.0)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yan,,Zheng
| | Sample_contact_email | zheng418@hotmail.com
| | Sample_contact_institute | University of Chicago
| | Sample_contact_address | 929 E. 57th St.
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126965/suppl/GSM1126965_An_Cre_01.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126965/suppl/GSM1126965_An_Cre_01.CHP.gz
| | Sample_series_id | GSE46242
| | Sample_series_id | GSE46243
| | Sample_data_row_count | 45101
| | |
|
GSM1126966 | GPL1261 |
|
Control EV rep2
|
Control wild-type Th1 T cells
|
strain: C57BL/6
cell type: Th1 T cells
genotype/variation: wild-type
treatment: untreated (Control)
|
Gene expression data from control wild-type Th1 T cells
Ctrl EV 02
|
| Sample_geo_accession | GSM1126966
| | Sample_status | Public on Apr 21 2013
| | Sample_submission_date | Apr 20 2013
| | Sample_last_update_date | Apr 21 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | CAR Tg x Egr2flox/flox Th1 T cells were infected with an empty (EV) or a Cre-expressing adenovirus, Egr2 deletion was confirmed by immunoblot. The T cells were then left untreated (Control) or anergized (Anergic) by immobilized anti-CD3 for 16 hours, and microarray was conducted after 1 day of rest in culture medium.
| | Sample_growth_protocol_ch1 | OVA-specific Th1 cell clones were generated from CAR Tg x Egr2flox/flox mice.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was purified using RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The RNAs were labeled with biotin and fragmented using GeneChip 3’ IVT Express Kit according to Affymetrix protocol.
| | Sample_hyb_protocol | The fragmented cRNA (12 µg) was then hybridized to Affymetrix mouse genome 430 2.0 expression arrays for 16 h at 45°C with 60 rpm in Affymetrix hybridization oven 640.
| | Sample_scan_protocol | The arrays were scanned by Affymetrix Gene Chip Scanner 3000 7G and CEL.
| | Sample_data_processing | Intensity files were generated by Gene Chip Operating Software v. 1.4 (MicroArray Suite 5.0)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yan,,Zheng
| | Sample_contact_email | zheng418@hotmail.com
| | Sample_contact_institute | University of Chicago
| | Sample_contact_address | 929 E. 57th St.
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126966/suppl/GSM1126966_Ctrl_EV_02.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126966/suppl/GSM1126966_Ctrl_EV_02.CHP.gz
| | Sample_series_id | GSE46242
| | Sample_series_id | GSE46243
| | Sample_data_row_count | 45101
| | |
|
GSM1126967 | GPL1261 |
|
Control Cre rep2
|
Control Egr2-deleted Th1 T cells
|
strain: C57BL/6
cell type: Th1 T cells
genotype/variation: Egr2-deleted
treatment: untreated (Control)
|
Gene expressoin data from control Egr2-deleted Th1 T cells
Ctrl Cre 02
|
| Sample_geo_accession | GSM1126967
| | Sample_status | Public on Apr 21 2013
| | Sample_submission_date | Apr 20 2013
| | Sample_last_update_date | Apr 21 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | CAR Tg x Egr2flox/flox Th1 T cells were infected with an empty (EV) or a Cre-expressing adenovirus, Egr2 deletion was confirmed by immunoblot. The T cells were then left untreated (Control) or anergized (Anergic) by immobilized anti-CD3 for 16 hours, and microarray was conducted after 1 day of rest in culture medium.
| | Sample_growth_protocol_ch1 | OVA-specific Th1 cell clones were generated from CAR Tg x Egr2flox/flox mice.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was purified using RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The RNAs were labeled with biotin and fragmented using GeneChip 3’ IVT Express Kit according to Affymetrix protocol.
| | Sample_hyb_protocol | The fragmented cRNA (12 µg) was then hybridized to Affymetrix mouse genome 430 2.0 expression arrays for 16 h at 45°C with 60 rpm in Affymetrix hybridization oven 640.
| | Sample_scan_protocol | The arrays were scanned by Affymetrix Gene Chip Scanner 3000 7G and CEL.
| | Sample_data_processing | Intensity files were generated by Gene Chip Operating Software v. 1.4 (MicroArray Suite 5.0)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yan,,Zheng
| | Sample_contact_email | zheng418@hotmail.com
| | Sample_contact_institute | University of Chicago
| | Sample_contact_address | 929 E. 57th St.
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126967/suppl/GSM1126967_Ctrl_Cre_02.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126967/suppl/GSM1126967_Ctrl_Cre_02.CHP.gz
| | Sample_series_id | GSE46242
| | Sample_series_id | GSE46243
| | Sample_data_row_count | 45101
| | |
|
GSM1126968 | GPL1261 |
|
Anergic EV rep2
|
Anergic wild-type Th1 T cells
|
strain: C57BL/6
cell type: Th1 T cells
genotype/variation: wild-type
treatment: immobilized anti-CD3
|
Gene expression data from anergic wild-type Th1 T cells
An EV 02
|
| Sample_geo_accession | GSM1126968
| | Sample_status | Public on Apr 21 2013
| | Sample_submission_date | Apr 20 2013
| | Sample_last_update_date | Apr 21 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | CAR Tg x Egr2flox/flox Th1 T cells were infected with an empty (EV) or a Cre-expressing adenovirus, Egr2 deletion was confirmed by immunoblot. The T cells were then left untreated (Control) or anergized (Anergic) by immobilized anti-CD3 for 16 hours, and microarray was conducted after 1 day of rest in culture medium.
| | Sample_growth_protocol_ch1 | OVA-specific Th1 cell clones were generated from CAR Tg x Egr2flox/flox mice.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was purified using RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The RNAs were labeled with biotin and fragmented using GeneChip 3’ IVT Express Kit according to Affymetrix protocol.
| | Sample_hyb_protocol | The fragmented cRNA (12 µg) was then hybridized to Affymetrix mouse genome 430 2.0 expression arrays for 16 h at 45°C with 60 rpm in Affymetrix hybridization oven 640.
| | Sample_scan_protocol | The arrays were scanned by Affymetrix Gene Chip Scanner 3000 7G and CEL.
| | Sample_data_processing | Intensity files were generated by Gene Chip Operating Software v. 1.4 (MicroArray Suite 5.0)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yan,,Zheng
| | Sample_contact_email | zheng418@hotmail.com
| | Sample_contact_institute | University of Chicago
| | Sample_contact_address | 929 E. 57th St.
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126968/suppl/GSM1126968_An_EV_02.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126968/suppl/GSM1126968_An_EV_02.CHP.gz
| | Sample_series_id | GSE46242
| | Sample_series_id | GSE46243
| | Sample_data_row_count | 45101
| | |
|
GSM1126969 | GPL1261 |
|
Anergic Cre rep2
|
Anergic Egr2-deleted Th1 T cells
|
strain: C57BL/6
cell type: Th1 T cells
genotype/variation: Egr2-deleted
treatment: immobilized anti-CD3
|
Gene expression data from anergic Egr2-deleted Th1 T cells
An Cre 02
|
| Sample_geo_accession | GSM1126969
| | Sample_status | Public on Apr 21 2013
| | Sample_submission_date | Apr 20 2013
| | Sample_last_update_date | Apr 21 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | CAR Tg x Egr2flox/flox Th1 T cells were infected with an empty (EV) or a Cre-expressing adenovirus, Egr2 deletion was confirmed by immunoblot. The T cells were then left untreated (Control) or anergized (Anergic) by immobilized anti-CD3 for 16 hours, and microarray was conducted after 1 day of rest in culture medium.
| | Sample_growth_protocol_ch1 | OVA-specific Th1 cell clones were generated from CAR Tg x Egr2flox/flox mice.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was purified using RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The RNAs were labeled with biotin and fragmented using GeneChip 3’ IVT Express Kit according to Affymetrix protocol.
| | Sample_hyb_protocol | The fragmented cRNA (12 µg) was then hybridized to Affymetrix mouse genome 430 2.0 expression arrays for 16 h at 45°C with 60 rpm in Affymetrix hybridization oven 640.
| | Sample_scan_protocol | The arrays were scanned by Affymetrix Gene Chip Scanner 3000 7G and CEL.
| | Sample_data_processing | Intensity files were generated by Gene Chip Operating Software v. 1.4 (MicroArray Suite 5.0)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yan,,Zheng
| | Sample_contact_email | zheng418@hotmail.com
| | Sample_contact_institute | University of Chicago
| | Sample_contact_address | 929 E. 57th St.
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126969/suppl/GSM1126969_An_Cre_02.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126969/suppl/GSM1126969_An_Cre_02.CHP.gz
| | Sample_series_id | GSE46242
| | Sample_series_id | GSE46243
| | Sample_data_row_count | 45101
| | |
|
GSM1126970 | GPL1261 |
|
Control EV rep3
|
Control wild-type Th1 T cells
|
strain: C57BL/6
cell type: Th1 T cells
genotype/variation: wild-type
treatment: untreated (Control)
|
Gene expression data from control wild-type Th1 T cells
Ctrl EV 03
|
| Sample_geo_accession | GSM1126970
| | Sample_status | Public on Apr 21 2013
| | Sample_submission_date | Apr 20 2013
| | Sample_last_update_date | Apr 21 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | CAR Tg x Egr2flox/flox Th1 T cells were infected with an empty (EV) or a Cre-expressing adenovirus, Egr2 deletion was confirmed by immunoblot. The T cells were then left untreated (Control) or anergized (Anergic) by immobilized anti-CD3 for 16 hours, and microarray was conducted after 1 day of rest in culture medium.
| | Sample_growth_protocol_ch1 | OVA-specific Th1 cell clones were generated from CAR Tg x Egr2flox/flox mice.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was purified using RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The RNAs were labeled with biotin and fragmented using GeneChip 3’ IVT Express Kit according to Affymetrix protocol.
| | Sample_hyb_protocol | The fragmented cRNA (12 µg) was then hybridized to Affymetrix mouse genome 430 2.0 expression arrays for 16 h at 45°C with 60 rpm in Affymetrix hybridization oven 640.
| | Sample_scan_protocol | The arrays were scanned by Affymetrix Gene Chip Scanner 3000 7G and CEL.
| | Sample_data_processing | Intensity files were generated by Gene Chip Operating Software v. 1.4 (MicroArray Suite 5.0)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yan,,Zheng
| | Sample_contact_email | zheng418@hotmail.com
| | Sample_contact_institute | University of Chicago
| | Sample_contact_address | 929 E. 57th St.
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126970/suppl/GSM1126970_Ctrl_EV_03.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126970/suppl/GSM1126970_Ctrl_EV_03.CHP.gz
| | Sample_series_id | GSE46242
| | Sample_series_id | GSE46243
| | Sample_data_row_count | 45101
| | |
|
GSM1126971 | GPL1261 |
|
Control Cre rep3
|
Control Egr2-deleted Th1 T cells
|
strain: C57BL/6
cell type: Th1 T cells
genotype/variation: Egr2-deleted
treatment: untreated (Control)
|
Gene expressoin data from control Egr2-deleted Th1 T cells
Ctrl Cre 03
|
| Sample_geo_accession | GSM1126971
| | Sample_status | Public on Apr 21 2013
| | Sample_submission_date | Apr 20 2013
| | Sample_last_update_date | Apr 21 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | CAR Tg x Egr2flox/flox Th1 T cells were infected with an empty (EV) or a Cre-expressing adenovirus, Egr2 deletion was confirmed by immunoblot. The T cells were then left untreated (Control) or anergized (Anergic) by immobilized anti-CD3 for 16 hours, and microarray was conducted after 1 day of rest in culture medium.
| | Sample_growth_protocol_ch1 | OVA-specific Th1 cell clones were generated from CAR Tg x Egr2flox/flox mice.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was purified using RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The RNAs were labeled with biotin and fragmented using GeneChip 3’ IVT Express Kit according to Affymetrix protocol.
| | Sample_hyb_protocol | The fragmented cRNA (12 µg) was then hybridized to Affymetrix mouse genome 430 2.0 expression arrays for 16 h at 45°C with 60 rpm in Affymetrix hybridization oven 640.
| | Sample_scan_protocol | The arrays were scanned by Affymetrix Gene Chip Scanner 3000 7G and CEL.
| | Sample_data_processing | Intensity files were generated by Gene Chip Operating Software v. 1.4 (MicroArray Suite 5.0)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yan,,Zheng
| | Sample_contact_email | zheng418@hotmail.com
| | Sample_contact_institute | University of Chicago
| | Sample_contact_address | 929 E. 57th St.
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126971/suppl/GSM1126971_Ctrl_Cre_03.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126971/suppl/GSM1126971_Ctrl_Cre_03.CHP.gz
| | Sample_series_id | GSE46242
| | Sample_series_id | GSE46243
| | Sample_data_row_count | 45101
| | |
|
GSM1126972 | GPL1261 |
|
Anergic EV rep3
|
Anergic wild-type Th1 T cells
|
strain: C57BL/6
cell type: Th1 T cells
genotype/variation: wild-type
treatment: immobilized anti-CD3
|
Gene expression data from anergic wild-type Th1 T cells
An EV 03
|
| Sample_geo_accession | GSM1126972
| | Sample_status | Public on Apr 21 2013
| | Sample_submission_date | Apr 20 2013
| | Sample_last_update_date | Apr 21 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | CAR Tg x Egr2flox/flox Th1 T cells were infected with an empty (EV) or a Cre-expressing adenovirus, Egr2 deletion was confirmed by immunoblot. The T cells were then left untreated (Control) or anergized (Anergic) by immobilized anti-CD3 for 16 hours, and microarray was conducted after 1 day of rest in culture medium.
| | Sample_growth_protocol_ch1 | OVA-specific Th1 cell clones were generated from CAR Tg x Egr2flox/flox mice.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was purified using RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The RNAs were labeled with biotin and fragmented using GeneChip 3’ IVT Express Kit according to Affymetrix protocol.
| | Sample_hyb_protocol | The fragmented cRNA (12 µg) was then hybridized to Affymetrix mouse genome 430 2.0 expression arrays for 16 h at 45°C with 60 rpm in Affymetrix hybridization oven 640.
| | Sample_scan_protocol | The arrays were scanned by Affymetrix Gene Chip Scanner 3000 7G and CEL.
| | Sample_data_processing | Intensity files were generated by Gene Chip Operating Software v. 1.4 (MicroArray Suite 5.0)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yan,,Zheng
| | Sample_contact_email | zheng418@hotmail.com
| | Sample_contact_institute | University of Chicago
| | Sample_contact_address | 929 E. 57th St.
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126972/suppl/GSM1126972_An_EV_03.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126972/suppl/GSM1126972_An_EV_03.CHP.gz
| | Sample_series_id | GSE46242
| | Sample_series_id | GSE46243
| | Sample_data_row_count | 45101
| | |
|
GSM1126973 | GPL1261 |
|
Anergic Cre rep3
|
Anergic Egr2-deleted Th1 T cells
|
strain: C57BL/6
cell type: Th1 T cells
genotype/variation: Egr2-deleted
treatment: immobilized anti-CD3
|
Gene expression data from anergic Egr2-deleted Th1 T cells
An Cre 03
|
| Sample_geo_accession | GSM1126973
| | Sample_status | Public on Apr 21 2013
| | Sample_submission_date | Apr 20 2013
| | Sample_last_update_date | Apr 21 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | CAR Tg x Egr2flox/flox Th1 T cells were infected with an empty (EV) or a Cre-expressing adenovirus, Egr2 deletion was confirmed by immunoblot. The T cells were then left untreated (Control) or anergized (Anergic) by immobilized anti-CD3 for 16 hours, and microarray was conducted after 1 day of rest in culture medium.
| | Sample_growth_protocol_ch1 | OVA-specific Th1 cell clones were generated from CAR Tg x Egr2flox/flox mice.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was purified using RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The RNAs were labeled with biotin and fragmented using GeneChip 3’ IVT Express Kit according to Affymetrix protocol.
| | Sample_hyb_protocol | The fragmented cRNA (12 µg) was then hybridized to Affymetrix mouse genome 430 2.0 expression arrays for 16 h at 45°C with 60 rpm in Affymetrix hybridization oven 640.
| | Sample_scan_protocol | The arrays were scanned by Affymetrix Gene Chip Scanner 3000 7G and CEL.
| | Sample_data_processing | Intensity files were generated by Gene Chip Operating Software v. 1.4 (MicroArray Suite 5.0)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yan,,Zheng
| | Sample_contact_email | zheng418@hotmail.com
| | Sample_contact_institute | University of Chicago
| | Sample_contact_address | 929 E. 57th St.
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126973/suppl/GSM1126973_An_Cre_03.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1126nnn/GSM1126973/suppl/GSM1126973_An_Cre_03.CHP.gz
| | Sample_series_id | GSE46242
| | Sample_series_id | GSE46243
| | Sample_data_row_count | 45101
| | |
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