Result

Search results for the GEO ID: GSE46326

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM1128654

GPL570

Pregnant FTDC culture without PIF

Pooled triplicate FTDC culture without PIF

cell type: FTDC mimicked developmental phase: Embryo implantation treatment: Control

Sample_geo_accession	GSM1128654
Sample_status	Public on Jun 21 2013
Sample_submission_date	Apr 23 2013
Sample_last_update_date	Jun 21 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were switched to a serum-free medium containing insulin, transferrin and selenium, 5umol/L trace elecments and 50ug/mL ascorbic acid, and treated for 24 hours with or without synthetic PIF at 100nmol/L.
Sample_growth_protocol_ch1	Discarded endometrial tissue from premenopausal women undergoing hysterectomies for benign indications were collected. Decidual specimens were collected from women undergoing elective terminations in weeks 6-12 of normal pregnancy. Endometral and decidual cells were isolated and resuspended in RPMI-1640, grown to confluence and confirmed to be leukocyte free. Cells were then decidualized using 10^-8mol/L estradiol and 10^-7mol/L synthetic progestin analog for 7 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA per the Affymetrix 3' IVT Express Kit User Manual.
Sample_hyb_protocol	Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Probe Array. GeneChips were washed and stained in the GeneChip Fluidics Station 450.
Sample_scan_protocol	Optical scanning with GeneChip Scanner 3000 (Affymetrix) at W. M. Keck Foundation Biotechnology Resource Laboratory, Yale University, New Haven, CT.
Sample_data_processing	Raw data were analyzed by GeneSpring software (Agilent, Santa Clara, CA, USA), normalized for inter-chip and intra-chip variation to eliminate false-positive results. The Sample data tables include MAS5 values. Fold-change data are linked to the Series record.
Sample_platform_id	GPL570
Sample_contact_name	Christina,Maria,Duzyj
Sample_contact_email	christina.duzyj@yale.edu
Sample_contact_department	Ob/Gyn: Maternal Fetal Medicine
Sample_contact_institute	Yale University
Sample_contact_address	333 Cedar St
Sample_contact_city	New Haven
Sample_contact_state	CT
Sample_contact_zip/postal_code	06520
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1128nnn/GSM1128654/suppl/GSM1128654_RPIC_133-2_FIDC-CONT_021908.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1128nnn/GSM1128654/suppl/GSM1128654_RPIC_133-2_FIDC-CONT_021908.mas5.CHP.gz
Sample_series_id	GSE46326
Sample_data_row_count	54675

GSM1128655

GPL570

Pregnant FTDC with PIF

Pooled triplicate FTDC culture with PIF

cell type: FTDC mimicked developmental phase: Embryo implantation treatment: PIF stimulation

Sample_geo_accession	GSM1128655
Sample_status	Public on Jun 21 2013
Sample_submission_date	Apr 23 2013
Sample_last_update_date	Jun 21 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were switched to a serum-free medium containing insulin, transferrin and selenium, 5umol/L trace elecments and 50ug/mL ascorbic acid, and treated for 24 hours with or without synthetic PIF at 100nmol/L.
Sample_growth_protocol_ch1	Discarded endometrial tissue from premenopausal women undergoing hysterectomies for benign indications were collected. Decidual specimens were collected from women undergoing elective terminations in weeks 6-12 of normal pregnancy. Endometral and decidual cells were isolated and resuspended in RPMI-1640, grown to confluence and confirmed to be leukocyte free. Cells were then decidualized using 10^-8mol/L estradiol and 10^-7mol/L synthetic progestin analog for 7 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA per the Affymetrix 3' IVT Express Kit User Manual.
Sample_hyb_protocol	Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Probe Array. GeneChips were washed and stained in the GeneChip Fluidics Station 450.
Sample_scan_protocol	Optical scanning with GeneChip Scanner 3000 (Affymetrix) at W. M. Keck Foundation Biotechnology Resource Laboratory, Yale University, New Haven, CT.
Sample_data_processing	Raw data were analyzed by GeneSpring software (Agilent, Santa Clara, CA, USA), normalized for inter-chip and intra-chip variation to eliminate false-positive results. The Sample data tables include MAS5 values. Fold-change data are linked to the Series record.
Sample_platform_id	GPL570
Sample_contact_name	Christina,Maria,Duzyj
Sample_contact_email	christina.duzyj@yale.edu
Sample_contact_department	Ob/Gyn: Maternal Fetal Medicine
Sample_contact_institute	Yale University
Sample_contact_address	333 Cedar St
Sample_contact_city	New Haven
Sample_contact_state	CT
Sample_contact_zip/postal_code	06520
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1128nnn/GSM1128655/suppl/GSM1128655_RPIC_133-2_FIDC-PIF_021908.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1128nnn/GSM1128655/suppl/GSM1128655_RPIC_133-2_FIDC-PIF_021908.mas5.CHP.gz
Sample_series_id	GSE46326
Sample_data_row_count	54675

GSM1128656

GPL570

Non-pregnant HESC culture without PIF

Pooled triplicate HESC culture without PIF

cell type: HESC mimicked developmental phase: Embryogenesis treatment: Control

Sample_geo_accession	GSM1128656
Sample_status	Public on Jun 21 2013
Sample_submission_date	Apr 23 2013
Sample_last_update_date	Jun 21 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were switched to a serum-free medium containing insulin, transferrin and selenium, 5umol/L trace elecments and 50ug/mL ascorbic acid, and treated for 24 hours with or without synthetic PIF at 100nmol/L.
Sample_growth_protocol_ch1	Discarded endometrial tissue from premenopausal women undergoing hysterectomies for benign indications were collected. Decidual specimens were collected from women undergoing elective terminations in weeks 6-12 of normal pregnancy. Endometral and decidual cells were isolated and resuspended in RPMI-1640, grown to confluence and confirmed to be leukocyte free. Cells were then decidualized using 10^-8mol/L estradiol and 10^-7mol/L synthetic progestin analog for 7 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA per the Affymetrix 3' IVT Express Kit User Manual.
Sample_hyb_protocol	Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Probe Array. GeneChips were washed and stained in the GeneChip Fluidics Station 450.
Sample_scan_protocol	Optical scanning with GeneChip Scanner 3000 (Affymetrix) at W. M. Keck Foundation Biotechnology Resource Laboratory, Yale University, New Haven, CT.
Sample_data_processing	Raw data were analyzed by GeneSpring software (Agilent, Santa Clara, CA, USA), normalized for inter-chip and intra-chip variation to eliminate false-positive results. The Sample data tables include MAS5 values. Fold-change data are linked to the Series record.
Sample_platform_id	GPL570
Sample_contact_name	Christina,Maria,Duzyj
Sample_contact_email	christina.duzyj@yale.edu
Sample_contact_department	Ob/Gyn: Maternal Fetal Medicine
Sample_contact_institute	Yale University
Sample_contact_address	333 Cedar St
Sample_contact_city	New Haven
Sample_contact_state	CT
Sample_contact_zip/postal_code	06520
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1128nnn/GSM1128656/suppl/GSM1128656_RPIC_133-2_HEEC-CONT_021908.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1128nnn/GSM1128656/suppl/GSM1128656_RPIC_133-2_HEEC-CONT_021908.mas5.CHP.gz
Sample_series_id	GSE46326
Sample_data_row_count	54675

GSM1128657

GPL570

Non-pregnant HESC culture with PIF

Pooled triplicate HESC culture with PIF

cell type: HESC mimicked developmental phase: Embryogenesis treatment: PIF stimulation

Sample_geo_accession	GSM1128657
Sample_status	Public on Jun 21 2013
Sample_submission_date	Apr 23 2013
Sample_last_update_date	Jun 21 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were switched to a serum-free medium containing insulin, transferrin and selenium, 5umol/L trace elecments and 50ug/mL ascorbic acid, and treated for 24 hours with or without synthetic PIF at 100nmol/L.
Sample_growth_protocol_ch1	Discarded endometrial tissue from premenopausal women undergoing hysterectomies for benign indications were collected. Decidual specimens were collected from women undergoing elective terminations in weeks 6-12 of normal pregnancy. Endometral and decidual cells were isolated and resuspended in RPMI-1640, grown to confluence and confirmed to be leukocyte free. Cells were then decidualized using 10^-8mol/L estradiol and 10^-7mol/L synthetic progestin analog for 7 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA per the Affymetrix 3' IVT Express Kit User Manual.
Sample_hyb_protocol	Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Probe Array. GeneChips were washed and stained in the GeneChip Fluidics Station 450.
Sample_scan_protocol	Optical scanning with GeneChip Scanner 3000 (Affymetrix) at W. M. Keck Foundation Biotechnology Resource Laboratory, Yale University, New Haven, CT.
Sample_data_processing	Raw data were analyzed by GeneSpring software (Agilent, Santa Clara, CA, USA), normalized for inter-chip and intra-chip variation to eliminate false-positive results. The Sample data tables include MAS5 values. Fold-change data are linked to the Series record.
Sample_platform_id	GPL570
Sample_contact_name	Christina,Maria,Duzyj
Sample_contact_email	christina.duzyj@yale.edu
Sample_contact_department	Ob/Gyn: Maternal Fetal Medicine
Sample_contact_institute	Yale University
Sample_contact_address	333 Cedar St
Sample_contact_city	New Haven
Sample_contact_state	CT
Sample_contact_zip/postal_code	06520
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1128nnn/GSM1128657/suppl/GSM1128657_RPIC_133-2_HEEC-PIF_021908.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1128nnn/GSM1128657/suppl/GSM1128657_RPIC_133-2_HEEC-PIF_021908.mas5.CHP.gz
Sample_series_id	GSE46326
Sample_data_row_count	54675

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