Search results for the GEO ID: GSE46390  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1129665 | GPL1261 |
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Cells with no CSF1 treatment
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Cells with no CSF1 treatment
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treatment: control
cell type: Mouse peritoneal macrophages
strain: C57BL6
age: 8 weeks
gender: male
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Gene expression data from macrophages without CSF1 treatment
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| Sample_geo_accession | GSM1129665
| | Sample_status | Public on Apr 30 2013
| | Sample_submission_date | Apr 25 2013
| | Sample_last_update_date | Apr 30 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | At 80% confluence, cells were treated with 100ng/ml CSF1 or without treatment for 12 hours and RNA extracted.
| | Sample_growth_protocol_ch1 | Wild type C57/BL6 mice were treated with 0.2 ml 10 % thioglycolate medium (FTG) / mouse by intra-peritoneal injection. Mice were sacrificed one week after injection followed by intra-peritoneal lavage with 15ml PBS. Macrophages were plated at a density of 1.5×10^6/well in 6-well plates and cultured in α-MEM supplemented with 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy kit (Qiagen, Gaithersburg, MD) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocols for Mouse Genome 430 2.0 Array
| | Sample_hyb_protocol | Standard Affymetrix protocols for Mouse Genome 430 2.0 Array
| | Sample_scan_protocol | Standard Affymetrix protocols for Mouse Genome 430 2.0 Array
| | Sample_data_processing | The data were analyzed with Gene spring 6.2 software using default parameters
| | Sample_platform_id | GPL1261
| | Sample_contact_name | chen,,yao
| | Sample_contact_email | chen.yao@yale.edu
| | Sample_contact_institute | yale
| | Sample_contact_address | 300 cedar street
| | Sample_contact_city | new haven
| | Sample_contact_zip/postal_code | 06520
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1129nnn/GSM1129665/suppl/GSM1129665_GYAO_430-2_WT0H_110205.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1129nnn/GSM1129665/suppl/GSM1129665_GYAO_430-2_WT0H_110205.CHP.gz
| | Sample_series_id | GSE46390
| | Sample_data_row_count | 45101
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GSM1129666 | GPL1261 |
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Cells with 12hrs CSF1 treatment
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Cells with 12hrs CSF1 treatment
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treatment: CSF1
cell type: Mouse peritoneal macrophages
strain: C57BL6
age: 8 weeks
gender: male
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Gene expression data from macrophages with 12hr CSF1 treatment
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| Sample_geo_accession | GSM1129666
| | Sample_status | Public on Apr 30 2013
| | Sample_submission_date | Apr 25 2013
| | Sample_last_update_date | Apr 30 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | At 80% confluence, cells were treated with 100ng/ml CSF1 or without treatment for 12 hours and RNA extracted.
| | Sample_growth_protocol_ch1 | Wild type C57/BL6 mice were treated with 0.2 ml 10 % thioglycolate medium (FTG) / mouse by intra-peritoneal injection. Mice were sacrificed one week after injection followed by intra-peritoneal lavage with 15ml PBS. Macrophages were plated at a density of 1.5×10^6/well in 6-well plates and cultured in α-MEM supplemented with 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy kit (Qiagen, Gaithersburg, MD) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocols for Mouse Genome 430 2.0 Array
| | Sample_hyb_protocol | Standard Affymetrix protocols for Mouse Genome 430 2.0 Array
| | Sample_scan_protocol | Standard Affymetrix protocols for Mouse Genome 430 2.0 Array
| | Sample_data_processing | The data were analyzed with Gene spring 6.2 software using default parameters
| | Sample_platform_id | GPL1261
| | Sample_contact_name | chen,,yao
| | Sample_contact_email | chen.yao@yale.edu
| | Sample_contact_institute | yale
| | Sample_contact_address | 300 cedar street
| | Sample_contact_city | new haven
| | Sample_contact_zip/postal_code | 06520
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1129nnn/GSM1129666/suppl/GSM1129666_GYAO_430-2_WT12H_110205.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1129nnn/GSM1129666/suppl/GSM1129666_GYAO_430-2_WT12H_110205.CHP.gz
| | Sample_series_id | GSE46390
| | Sample_data_row_count | 45101
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