Search results for the GEO ID: GSE46453  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1130579 | GPL1261 |
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Activated tumor-specific CD4+ T cells, biological rep1
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mouse tumor-specific CD4+ T cells, activated
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tissue: tumor-specific CD4+ T cells
cell type: mouse tumor-specific CD4+ T cells, activated
background strain: Balb/c
age: adult (7-12 weeks)
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Day +8 in vivo activated
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| Sample_geo_accession | GSM1130579
| | Sample_status | Public on May 15 2013
| | Sample_submission_date | Apr 29 2013
| | Sample_last_update_date | May 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | TCR-transgenic mice were challenged s.c. with MOPC315 myeloma cells.
| | Sample_growth_protocol_ch1 | in vivo activated T cells.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G (Affymetrix).
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software 1.3 (Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Alexandre,,Corthay
| | Sample_contact_email | alexandre.corthay@medisin.uio.no
| | Sample_contact_laboratory | Tumor Immunology Research Group
| | Sample_contact_department | Department of Biosciences
| | Sample_contact_institute | University of Oslo
| | Sample_contact_address | Rikshospitalet
| | Sample_contact_city | Oslo
| | Sample_contact_zip/postal_code | P.O. Box 4950 Nydalen, 0424
| | Sample_contact_country | Norway
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1130nnn/GSM1130579/suppl/GSM1130579_Activated_T_cells_sample_1.CEL.gz
| | Sample_series_id | GSE46453
| | Sample_data_row_count | 45101
| | |
|
GSM1130580 | GPL1261 |
|
Activated tumor-specific CD4+ T cells, biological rep2
|
mouse tumor-specific CD4+ T cells, activated
|
tissue: tumor-specific CD4+ T cells
cell type: mouse tumor-specific CD4+ T cells, activated
background strain: Balb/c
age: adult (7-12 weeks)
|
Day +8 in vivo activated
|
| Sample_geo_accession | GSM1130580
| | Sample_status | Public on May 15 2013
| | Sample_submission_date | Apr 29 2013
| | Sample_last_update_date | May 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | TCR-transgenic mice were challenged s.c. with MOPC315 myeloma cells.
| | Sample_growth_protocol_ch1 | in vivo activated T cells.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G (Affymetrix).
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software 1.3 (Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Alexandre,,Corthay
| | Sample_contact_email | alexandre.corthay@medisin.uio.no
| | Sample_contact_laboratory | Tumor Immunology Research Group
| | Sample_contact_department | Department of Biosciences
| | Sample_contact_institute | University of Oslo
| | Sample_contact_address | Rikshospitalet
| | Sample_contact_city | Oslo
| | Sample_contact_zip/postal_code | P.O. Box 4950 Nydalen, 0424
| | Sample_contact_country | Norway
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1130nnn/GSM1130580/suppl/GSM1130580_Activated_T_cells_sample_2.CEL.gz
| | Sample_series_id | GSE46453
| | Sample_data_row_count | 45101
| | |
|
GSM1130581 | GPL1261 |
|
Activated tumor-specific CD4+ T cells, biological rep3
|
mouse tumor-specific CD4+ T cells, activated
|
tissue: tumor-specific CD4+ T cells
cell type: mouse tumor-specific CD4+ T cells, activated
background strain: Balb/c
age: adult (7-12 weeks)
|
Day +8 in vivo activated
|
| Sample_geo_accession | GSM1130581
| | Sample_status | Public on May 15 2013
| | Sample_submission_date | Apr 29 2013
| | Sample_last_update_date | May 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | TCR-transgenic mice were challenged s.c. with MOPC315 myeloma cells.
| | Sample_growth_protocol_ch1 | in vivo activated T cells.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G (Affymetrix).
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software 1.3 (Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Alexandre,,Corthay
| | Sample_contact_email | alexandre.corthay@medisin.uio.no
| | Sample_contact_laboratory | Tumor Immunology Research Group
| | Sample_contact_department | Department of Biosciences
| | Sample_contact_institute | University of Oslo
| | Sample_contact_address | Rikshospitalet
| | Sample_contact_city | Oslo
| | Sample_contact_zip/postal_code | P.O. Box 4950 Nydalen, 0424
| | Sample_contact_country | Norway
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1130nnn/GSM1130581/suppl/GSM1130581_Activated_T_cells_sample_3.CEL.gz
| | Sample_series_id | GSE46453
| | Sample_data_row_count | 45101
| | |
|
GSM1130582 | GPL1261 |
|
Naive tumor-specific CD4+ T cells, biological rep1
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mouse tumor-specific CD4+ T cells, non activated
|
tissue: tumor-specific CD4+ T cells
cell type: mouse tumor-specific CD4+ T cells, non activated
background strain: Balb/c
age: adult (7-12 weeks)
|
Non activated (from naive mice)
|
| Sample_geo_accession | GSM1130582
| | Sample_status | Public on May 15 2013
| | Sample_submission_date | Apr 29 2013
| | Sample_last_update_date | May 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | TCR-transgenic mice were challenged s.c. with MOPC315 myeloma cells.
| | Sample_growth_protocol_ch1 | in vivo activated T cells.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G (Affymetrix).
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software 1.3 (Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Alexandre,,Corthay
| | Sample_contact_email | alexandre.corthay@medisin.uio.no
| | Sample_contact_laboratory | Tumor Immunology Research Group
| | Sample_contact_department | Department of Biosciences
| | Sample_contact_institute | University of Oslo
| | Sample_contact_address | Rikshospitalet
| | Sample_contact_city | Oslo
| | Sample_contact_zip/postal_code | P.O. Box 4950 Nydalen, 0424
| | Sample_contact_country | Norway
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1130nnn/GSM1130582/suppl/GSM1130582_Naive_T_cells_sample_1.CEL.gz
| | Sample_series_id | GSE46453
| | Sample_data_row_count | 45101
| | |
|
GSM1130583 | GPL1261 |
|
Naive tumor-specific CD4+ T cells, biological rep2
|
mouse tumor-specific CD4+ T cells, non activated
|
tissue: tumor-specific CD4+ T cells
cell type: mouse tumor-specific CD4+ T cells, non activated
background strain: Balb/c
age: adult (7-12 weeks)
|
Non activated (from naive mice)
|
| Sample_geo_accession | GSM1130583
| | Sample_status | Public on May 15 2013
| | Sample_submission_date | Apr 29 2013
| | Sample_last_update_date | May 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | TCR-transgenic mice were challenged s.c. with MOPC315 myeloma cells.
| | Sample_growth_protocol_ch1 | in vivo activated T cells.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G (Affymetrix).
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software 1.3 (Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Alexandre,,Corthay
| | Sample_contact_email | alexandre.corthay@medisin.uio.no
| | Sample_contact_laboratory | Tumor Immunology Research Group
| | Sample_contact_department | Department of Biosciences
| | Sample_contact_institute | University of Oslo
| | Sample_contact_address | Rikshospitalet
| | Sample_contact_city | Oslo
| | Sample_contact_zip/postal_code | P.O. Box 4950 Nydalen, 0424
| | Sample_contact_country | Norway
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1130nnn/GSM1130583/suppl/GSM1130583_Naive_T_cells_sample_2.CEL.gz
| | Sample_series_id | GSE46453
| | Sample_data_row_count | 45101
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