Result

Search results for the GEO ID: GSE46496

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM1131308

GPL1261

Atria, Mutant, biological replicate 1

Mutant right atria

tissue: atria genotype: Myh6-cre; COUP-TFIIflox/flox age: 2 months genetic background: C57BL/6

Sample_geo_accession	GSM1131308
Sample_status	Public on Jun 07 2013
Sample_submission_date	Apr 30 2013
Sample_last_update_date	Jun 07 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction followed by Qiagen RNeasy purification
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Samples were labeled using the standard Affymetrix linear amplification protocol using the 3’ IVT Express Kit. 250 ng of total RNA was reverse transcribed to produce double-stranded cDNA. The cDNA product was used as a template for the vitro transcription reaction, producing biotin-labeled cRNA. The labeled cRNA was tested for integrity on the Agilent Bioanalyzer and quantified using the NanoDrop® ND-1000 spectrophotometer. 15.0 µg of the labeled cRNA was fragmented and re-checked for concentration and size.
Sample_hyb_protocol	Hybridization cocktails containing Affymetrix spike-in controls and 15.0ug of each fragmented, labeled cRNA were loaded onto Affymetrix GeneChip® Mouse 430 2.0 arrays. The arrays were hybridized for 16 hours at 45°C with rotation at 60 rpm in the Affymetrix GeneChip® Hybridization Oven 640. The arrays were washed and stained with a streptavidin, R-phycoerythrin conjugate stain using the Affymetrix GeneChip® Fluidics Station 450 using fluidics protocol FS450_0001. Signal amplification was done using biotinylated antistreptavidin.
Sample_scan_protocol	The stained arrays were scanned on the Affymetrix GeneChip® Scanner 3000.
Sample_data_processing	The data were analyzed with GeneSpring 12.5 GX using default analysis settings with RMA as the summerization algorithm.
Sample_platform_id	GPL1261
Sample_contact_name	San-pin,,Wu
Sample_contact_institute	Baylor College of Medicine
Sample_contact_address	1 Baylor Plaza
Sample_contact_city	Houston
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1131nnn/GSM1131308/suppl/GSM1131308_irs_MT_7361_MA2_28099.CEL.gz
Sample_series_id	GSE46496
Sample_series_id	GSE46498
Sample_data_row_count	45101

GSM1131309

GPL1261

Atria, Mutant, biological replicate 2

Mutant right atria

tissue: atria genotype: Myh6-cre; COUP-TFIIflox/flox age: 2 months genetic background: C57BL/6

Sample_geo_accession	GSM1131309
Sample_status	Public on Jun 07 2013
Sample_submission_date	Apr 30 2013
Sample_last_update_date	Jun 07 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction followed by Qiagen RNeasy purification
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Samples were labeled using the standard Affymetrix linear amplification protocol using the 3’ IVT Express Kit. 250 ng of total RNA was reverse transcribed to produce double-stranded cDNA. The cDNA product was used as a template for the vitro transcription reaction, producing biotin-labeled cRNA. The labeled cRNA was tested for integrity on the Agilent Bioanalyzer and quantified using the NanoDrop® ND-1000 spectrophotometer. 15.0 µg of the labeled cRNA was fragmented and re-checked for concentration and size.
Sample_hyb_protocol	Hybridization cocktails containing Affymetrix spike-in controls and 15.0ug of each fragmented, labeled cRNA were loaded onto Affymetrix GeneChip® Mouse 430 2.0 arrays. The arrays were hybridized for 16 hours at 45°C with rotation at 60 rpm in the Affymetrix GeneChip® Hybridization Oven 640. The arrays were washed and stained with a streptavidin, R-phycoerythrin conjugate stain using the Affymetrix GeneChip® Fluidics Station 450 using fluidics protocol FS450_0001. Signal amplification was done using biotinylated antistreptavidin.
Sample_scan_protocol	The stained arrays were scanned on the Affymetrix GeneChip® Scanner 3000.
Sample_data_processing	The data were analyzed with GeneSpring 12.5 GX using default analysis settings with RMA as the summerization algorithm.
Sample_platform_id	GPL1261
Sample_contact_name	San-pin,,Wu
Sample_contact_institute	Baylor College of Medicine
Sample_contact_address	1 Baylor Plaza
Sample_contact_city	Houston
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1131nnn/GSM1131309/suppl/GSM1131309_irs_MT_7361_MA2_28218.CEL.gz
Sample_series_id	GSE46496
Sample_series_id	GSE46498
Sample_data_row_count	45101

GSM1131310

GPL1261

Atria, Mutant, biological replicate 3

Mutant right atria

tissue: atria genotype: Myh6-cre; COUP-TFIIflox/flox age: 2 months genetic background: C57BL/6

Sample_geo_accession	GSM1131310
Sample_status	Public on Jun 07 2013
Sample_submission_date	Apr 30 2013
Sample_last_update_date	Jun 07 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction followed by Qiagen RNeasy purification
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Samples were labeled using the standard Affymetrix linear amplification protocol using the 3’ IVT Express Kit. 250 ng of total RNA was reverse transcribed to produce double-stranded cDNA. The cDNA product was used as a template for the vitro transcription reaction, producing biotin-labeled cRNA. The labeled cRNA was tested for integrity on the Agilent Bioanalyzer and quantified using the NanoDrop® ND-1000 spectrophotometer. 15.0 µg of the labeled cRNA was fragmented and re-checked for concentration and size.
Sample_hyb_protocol	Hybridization cocktails containing Affymetrix spike-in controls and 15.0ug of each fragmented, labeled cRNA were loaded onto Affymetrix GeneChip® Mouse 430 2.0 arrays. The arrays were hybridized for 16 hours at 45°C with rotation at 60 rpm in the Affymetrix GeneChip® Hybridization Oven 640. The arrays were washed and stained with a streptavidin, R-phycoerythrin conjugate stain using the Affymetrix GeneChip® Fluidics Station 450 using fluidics protocol FS450_0001. Signal amplification was done using biotinylated antistreptavidin.
Sample_scan_protocol	The stained arrays were scanned on the Affymetrix GeneChip® Scanner 3000.
Sample_data_processing	The data were analyzed with GeneSpring 12.5 GX using default analysis settings with RMA as the summerization algorithm.
Sample_platform_id	GPL1261
Sample_contact_name	San-pin,,Wu
Sample_contact_institute	Baylor College of Medicine
Sample_contact_address	1 Baylor Plaza
Sample_contact_city	Houston
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1131nnn/GSM1131310/suppl/GSM1131310_irs_MT_7361_MA2_28219.CEL.gz
Sample_series_id	GSE46496
Sample_series_id	GSE46498
Sample_data_row_count	45101

GSM1131311

GPL1261

Atria, Control, biological replicate 1

Control right atria

tissue: atria genotype: COUP-TFIIflox/flox age: 2 months genetic background: C57BL/6

Sample_geo_accession	GSM1131311
Sample_status	Public on Jun 07 2013
Sample_submission_date	Apr 30 2013
Sample_last_update_date	Jun 07 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction followed by Qiagen RNeasy purification
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Samples were labeled using the standard Affymetrix linear amplification protocol using the 3’ IVT Express Kit. 250 ng of total RNA was reverse transcribed to produce double-stranded cDNA. The cDNA product was used as a template for the vitro transcription reaction, producing biotin-labeled cRNA. The labeled cRNA was tested for integrity on the Agilent Bioanalyzer and quantified using the NanoDrop® ND-1000 spectrophotometer. 15.0 µg of the labeled cRNA was fragmented and re-checked for concentration and size.
Sample_hyb_protocol	Hybridization cocktails containing Affymetrix spike-in controls and 15.0ug of each fragmented, labeled cRNA were loaded onto Affymetrix GeneChip® Mouse 430 2.0 arrays. The arrays were hybridized for 16 hours at 45°C with rotation at 60 rpm in the Affymetrix GeneChip® Hybridization Oven 640. The arrays were washed and stained with a streptavidin, R-phycoerythrin conjugate stain using the Affymetrix GeneChip® Fluidics Station 450 using fluidics protocol FS450_0001. Signal amplification was done using biotinylated antistreptavidin.
Sample_scan_protocol	The stained arrays were scanned on the Affymetrix GeneChip® Scanner 3000.
Sample_data_processing	The data were analyzed with GeneSpring 12.5 GX using default analysis settings with RMA as the summerization algorithm.
Sample_platform_id	GPL1261
Sample_contact_name	San-pin,,Wu
Sample_contact_institute	Baylor College of Medicine
Sample_contact_address	1 Baylor Plaza
Sample_contact_city	Houston
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1131nnn/GSM1131311/suppl/GSM1131311_irs_MT_7361_MA2_28184.CEL.gz
Sample_series_id	GSE46496
Sample_series_id	GSE46498
Sample_data_row_count	45101

GSM1131312

GPL1261

Atria, Control, biological replicate 2

Control right atria

tissue: atria genotype: COUP-TFIIflox/flox age: 2 months genetic background: C57BL/6

Sample_geo_accession	GSM1131312
Sample_status	Public on Jun 07 2013
Sample_submission_date	Apr 30 2013
Sample_last_update_date	Jun 07 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction followed by Qiagen RNeasy purification
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Samples were labeled using the standard Affymetrix linear amplification protocol using the 3’ IVT Express Kit. 250 ng of total RNA was reverse transcribed to produce double-stranded cDNA. The cDNA product was used as a template for the vitro transcription reaction, producing biotin-labeled cRNA. The labeled cRNA was tested for integrity on the Agilent Bioanalyzer and quantified using the NanoDrop® ND-1000 spectrophotometer. 15.0 µg of the labeled cRNA was fragmented and re-checked for concentration and size.
Sample_hyb_protocol	Hybridization cocktails containing Affymetrix spike-in controls and 15.0ug of each fragmented, labeled cRNA were loaded onto Affymetrix GeneChip® Mouse 430 2.0 arrays. The arrays were hybridized for 16 hours at 45°C with rotation at 60 rpm in the Affymetrix GeneChip® Hybridization Oven 640. The arrays were washed and stained with a streptavidin, R-phycoerythrin conjugate stain using the Affymetrix GeneChip® Fluidics Station 450 using fluidics protocol FS450_0001. Signal amplification was done using biotinylated antistreptavidin.
Sample_scan_protocol	The stained arrays were scanned on the Affymetrix GeneChip® Scanner 3000.
Sample_data_processing	The data were analyzed with GeneSpring 12.5 GX using default analysis settings with RMA as the summerization algorithm.
Sample_platform_id	GPL1261
Sample_contact_name	San-pin,,Wu
Sample_contact_institute	Baylor College of Medicine
Sample_contact_address	1 Baylor Plaza
Sample_contact_city	Houston
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1131nnn/GSM1131312/suppl/GSM1131312_irs_MT_7361_MA2_28185.CEL.gz
Sample_series_id	GSE46496
Sample_series_id	GSE46498
Sample_data_row_count	45101

GSM1131313

GPL1261

Atria, Control, biological replicate 3

Control right atria

tissue: atria genotype: COUP-TFIIflox/flox age: 2 months genetic background: C57BL/6

Sample_geo_accession	GSM1131313
Sample_status	Public on Jun 07 2013
Sample_submission_date	Apr 30 2013
Sample_last_update_date	Jun 07 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction followed by Qiagen RNeasy purification
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Samples were labeled using the standard Affymetrix linear amplification protocol using the 3’ IVT Express Kit. 250 ng of total RNA was reverse transcribed to produce double-stranded cDNA. The cDNA product was used as a template for the vitro transcription reaction, producing biotin-labeled cRNA. The labeled cRNA was tested for integrity on the Agilent Bioanalyzer and quantified using the NanoDrop® ND-1000 spectrophotometer. 15.0 µg of the labeled cRNA was fragmented and re-checked for concentration and size.
Sample_hyb_protocol	Hybridization cocktails containing Affymetrix spike-in controls and 15.0ug of each fragmented, labeled cRNA were loaded onto Affymetrix GeneChip® Mouse 430 2.0 arrays. The arrays were hybridized for 16 hours at 45°C with rotation at 60 rpm in the Affymetrix GeneChip® Hybridization Oven 640. The arrays were washed and stained with a streptavidin, R-phycoerythrin conjugate stain using the Affymetrix GeneChip® Fluidics Station 450 using fluidics protocol FS450_0001. Signal amplification was done using biotinylated antistreptavidin.
Sample_scan_protocol	The stained arrays were scanned on the Affymetrix GeneChip® Scanner 3000.
Sample_data_processing	The data were analyzed with GeneSpring 12.5 GX using default analysis settings with RMA as the summerization algorithm.
Sample_platform_id	GPL1261
Sample_contact_name	San-pin,,Wu
Sample_contact_institute	Baylor College of Medicine
Sample_contact_address	1 Baylor Plaza
Sample_contact_city	Houston
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1131nnn/GSM1131313/suppl/GSM1131313_irs_MT_7361_MA2_28186.CEL.gz
Sample_series_id	GSE46496
Sample_series_id	GSE46498
Sample_data_row_count	45101

GSM1131314

GPL1261

Ventricles, Control, biological replicate 1

Control ventricles

tissue: ventricles genotype: COUP-TFIIflox/flox age: 2 months genetic background: C57BL/6

Sample_geo_accession	GSM1131314
Sample_status	Public on Jun 07 2013
Sample_submission_date	Apr 30 2013
Sample_last_update_date	Jun 07 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction followed by Qiagen RNeasy purification
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Samples were labeled using the standard Affymetrix linear amplification protocol using the 3’ IVT Express Kit. 250 ng of total RNA was reverse transcribed to produce double-stranded cDNA. The cDNA product was used as a template for the vitro transcription reaction, producing biotin-labeled cRNA. The labeled cRNA was tested for integrity on the Agilent Bioanalyzer and quantified using the NanoDrop® ND-1000 spectrophotometer. 15.0 µg of the labeled cRNA was fragmented and re-checked for concentration and size.
Sample_hyb_protocol	Hybridization cocktails containing Affymetrix spike-in controls and 15.0ug of each fragmented, labeled cRNA were loaded onto Affymetrix GeneChip® Mouse 430 2.0 arrays. The arrays were hybridized for 16 hours at 45°C with rotation at 60 rpm in the Affymetrix GeneChip® Hybridization Oven 640. The arrays were washed and stained with a streptavidin, R-phycoerythrin conjugate stain using the Affymetrix GeneChip® Fluidics Station 450 using fluidics protocol FS450_0001. Signal amplification was done using biotinylated antistreptavidin.
Sample_scan_protocol	The stained arrays were scanned on the Affymetrix GeneChip® Scanner 3000.
Sample_data_processing	The data were analyzed with GeneSpring 12.5 GX using default analysis settings with RMA as the summerization algorithm.
Sample_platform_id	GPL1261
Sample_contact_name	San-pin,,Wu
Sample_contact_institute	Baylor College of Medicine
Sample_contact_address	1 Baylor Plaza
Sample_contact_city	Houston
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1131nnn/GSM1131314/suppl/GSM1131314_irs_MT_7361_MA2_28102.CEL.gz
Sample_series_id	GSE46496
Sample_series_id	GSE46498
Sample_data_row_count	45101

GSM1131315

GPL1261

Ventricles, Control, biological replicate 2

Control ventricles

tissue: ventricles genotype: COUP-TFIIflox/flox age: 2 months genetic background: C57BL/6

Sample_geo_accession	GSM1131315
Sample_status	Public on Jun 07 2013
Sample_submission_date	Apr 30 2013
Sample_last_update_date	Jun 07 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction followed by Qiagen RNeasy purification
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Samples were labeled using the standard Affymetrix linear amplification protocol using the 3’ IVT Express Kit. 250 ng of total RNA was reverse transcribed to produce double-stranded cDNA. The cDNA product was used as a template for the vitro transcription reaction, producing biotin-labeled cRNA. The labeled cRNA was tested for integrity on the Agilent Bioanalyzer and quantified using the NanoDrop® ND-1000 spectrophotometer. 15.0 µg of the labeled cRNA was fragmented and re-checked for concentration and size.
Sample_hyb_protocol	Hybridization cocktails containing Affymetrix spike-in controls and 15.0ug of each fragmented, labeled cRNA were loaded onto Affymetrix GeneChip® Mouse 430 2.0 arrays. The arrays were hybridized for 16 hours at 45°C with rotation at 60 rpm in the Affymetrix GeneChip® Hybridization Oven 640. The arrays were washed and stained with a streptavidin, R-phycoerythrin conjugate stain using the Affymetrix GeneChip® Fluidics Station 450 using fluidics protocol FS450_0001. Signal amplification was done using biotinylated antistreptavidin.
Sample_scan_protocol	The stained arrays were scanned on the Affymetrix GeneChip® Scanner 3000.
Sample_data_processing	The data were analyzed with GeneSpring 12.5 GX using default analysis settings with RMA as the summerization algorithm.
Sample_platform_id	GPL1261
Sample_contact_name	San-pin,,Wu
Sample_contact_institute	Baylor College of Medicine
Sample_contact_address	1 Baylor Plaza
Sample_contact_city	Houston
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1131nnn/GSM1131315/suppl/GSM1131315_irs_MT_7361_MA2_28103.CEL.gz
Sample_series_id	GSE46496
Sample_series_id	GSE46498
Sample_data_row_count	45101

GSM1131316

GPL1261

Ventricles, Control, biological replicate 3

Control ventricles

tissue: ventricles genotype: COUP-TFIIflox/flox age: 2 months genetic background: C57BL/6

Sample_geo_accession	GSM1131316
Sample_status	Public on Jun 07 2013
Sample_submission_date	Apr 30 2013
Sample_last_update_date	Jun 07 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction followed by Qiagen RNeasy purification
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Samples were labeled using the standard Affymetrix linear amplification protocol using the 3’ IVT Express Kit. 250 ng of total RNA was reverse transcribed to produce double-stranded cDNA. The cDNA product was used as a template for the vitro transcription reaction, producing biotin-labeled cRNA. The labeled cRNA was tested for integrity on the Agilent Bioanalyzer and quantified using the NanoDrop® ND-1000 spectrophotometer. 15.0 µg of the labeled cRNA was fragmented and re-checked for concentration and size.
Sample_hyb_protocol	Hybridization cocktails containing Affymetrix spike-in controls and 15.0ug of each fragmented, labeled cRNA were loaded onto Affymetrix GeneChip® Mouse 430 2.0 arrays. The arrays were hybridized for 16 hours at 45°C with rotation at 60 rpm in the Affymetrix GeneChip® Hybridization Oven 640. The arrays were washed and stained with a streptavidin, R-phycoerythrin conjugate stain using the Affymetrix GeneChip® Fluidics Station 450 using fluidics protocol FS450_0001. Signal amplification was done using biotinylated antistreptavidin.
Sample_scan_protocol	The stained arrays were scanned on the Affymetrix GeneChip® Scanner 3000.
Sample_data_processing	The data were analyzed with GeneSpring 12.5 GX using default analysis settings with RMA as the summerization algorithm.
Sample_platform_id	GPL1261
Sample_contact_name	San-pin,,Wu
Sample_contact_institute	Baylor College of Medicine
Sample_contact_address	1 Baylor Plaza
Sample_contact_city	Houston
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1131nnn/GSM1131316/suppl/GSM1131316_irs_MT_7361_MA2_28104.CEL.gz
Sample_series_id	GSE46496
Sample_series_id	GSE46498
Sample_data_row_count	45101

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