Result

Search results for the GEO ID: GSE46528

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM1131786

GPL570

IKK1

IKK1_1

cell line: Huh7.5.1 treatment: treated with IKKalpha siRNA

Sample_geo_accession	GSM1131786
Sample_status	Public on May 08 2013
Sample_submission_date	Apr 30 2013
Sample_last_update_date	May 08 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were treated with either non-targeting control siRNA or siRNA against IKKalpha at a final concentration of 50 nM. After 72 h, cells were either mock infected or infected with HCV JFH-1 strain with the M.O.I. of 0.5. At 48 h post-infection, cells were harvested, and total cellular RNA was extracted using a QIAGEN RNeasy mini kit. Total RNA was quantified, measured for purity, and then subjected to microarray.
Sample_growth_protocol_ch1	All cell lines were growed with/ with no the HCV infection as in the reference.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	RMA by software Partek v6.5
Sample_platform_id	GPL570
Sample_contact_name	WeiPing,,Chen
Sample_contact_email	weipingChen@niddk.nih.gov
Sample_contact_phone	301-496-0175
Sample_contact_laboratory	Microarray Core Lab
Sample_contact_department	MCL
Sample_contact_institute	NIDDK/NIH
Sample_contact_address	Bldg 8, Room 1A11, NIDDK/NIH
Sample_contact_city	Bethesda
Sample_contact_state	MD
Sample_contact_zip/postal_code	20892
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1131nnn/GSM1131786/suppl/GSM1131786_01-27-10_JLiangIKK1_FQL_HG_U133Plus_2_IKK1.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1131nnn/GSM1131786/suppl/GSM1131786_01-27-10_JLiangIKK1_FQL_HG_U133Plus_2_IKK1.CHP.gz
Sample_series_id	GSE46528
Sample_data_row_count	54675

GSM1131787

GPL570

IKK2

IKK2_2

cell line: Huh7.5.1 treatment: treated with IKKalpha siRNA

Sample_geo_accession	GSM1131787
Sample_status	Public on May 08 2013
Sample_submission_date	Apr 30 2013
Sample_last_update_date	May 08 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were treated with either non-targeting control siRNA or siRNA against IKKalpha at a final concentration of 50 nM. After 72 h, cells were either mock infected or infected with HCV JFH-1 strain with the M.O.I. of 0.5. At 48 h post-infection, cells were harvested, and total cellular RNA was extracted using a QIAGEN RNeasy mini kit. Total RNA was quantified, measured for purity, and then subjected to microarray.
Sample_growth_protocol_ch1	All cell lines were growed with/ with no the HCV infection as in the reference.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	RMA by software Partek v6.5
Sample_platform_id	GPL570
Sample_contact_name	WeiPing,,Chen
Sample_contact_email	weipingChen@niddk.nih.gov
Sample_contact_phone	301-496-0175
Sample_contact_laboratory	Microarray Core Lab
Sample_contact_department	MCL
Sample_contact_institute	NIDDK/NIH
Sample_contact_address	Bldg 8, Room 1A11, NIDDK/NIH
Sample_contact_city	Bethesda
Sample_contact_state	MD
Sample_contact_zip/postal_code	20892
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1131nnn/GSM1131787/suppl/GSM1131787_01-27-10_JLiangIKK2_FQL_HG_U133Plus_2_IKK2.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1131nnn/GSM1131787/suppl/GSM1131787_01-27-10_JLiangIKK2_FQL_HG_U133Plus_2_IKK2.CHP.gz
Sample_series_id	GSE46528
Sample_data_row_count	54675

GSM1131789

GPL570

JIKK2

JIKK2_2

cell line: Huh7.5.1 treatment: treated with IKKalpha siRNA and subsequently infected with HCV

Sample_geo_accession	GSM1131789
Sample_status	Public on May 08 2013
Sample_submission_date	Apr 30 2013
Sample_last_update_date	May 08 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were treated with either non-targeting control siRNA or siRNA against IKKalpha at a final concentration of 50 nM. After 72 h, cells were either mock infected or infected with HCV JFH-1 strain with the M.O.I. of 0.5. At 48 h post-infection, cells were harvested, and total cellular RNA was extracted using a QIAGEN RNeasy mini kit. Total RNA was quantified, measured for purity, and then subjected to microarray.
Sample_growth_protocol_ch1	All cell lines were growed with/ with no the HCV infection as in the reference.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	RMA by software Partek v6.5
Sample_platform_id	GPL570
Sample_contact_name	WeiPing,,Chen
Sample_contact_email	weipingChen@niddk.nih.gov
Sample_contact_phone	301-496-0175
Sample_contact_laboratory	Microarray Core Lab
Sample_contact_department	MCL
Sample_contact_institute	NIDDK/NIH
Sample_contact_address	Bldg 8, Room 1A11, NIDDK/NIH
Sample_contact_city	Bethesda
Sample_contact_state	MD
Sample_contact_zip/postal_code	20892
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1131nnn/GSM1131789/suppl/GSM1131789_01-27-10_JLiangJIKK2_FQL_HG_U133Plus_2_JIKK2.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1131nnn/GSM1131789/suppl/GSM1131789_01-27-10_JLiangJIKK2_FQL_HG_U133Plus_2_JIKK2.CHP.gz
Sample_series_id	GSE46528
Sample_data_row_count	54675

GSM1131790

GPL570

JNT1

JNT1_1

cell line: Huh7.5.1 treatment: treated with non-targeting control siRNA then infected with HCV

Sample_geo_accession	GSM1131790
Sample_status	Public on May 08 2013
Sample_submission_date	Apr 30 2013
Sample_last_update_date	May 08 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were treated with either non-targeting control siRNA or siRNA against IKKalpha at a final concentration of 50 nM. After 72 h, cells were either mock infected or infected with HCV JFH-1 strain with the M.O.I. of 0.5. At 48 h post-infection, cells were harvested, and total cellular RNA was extracted using a QIAGEN RNeasy mini kit. Total RNA was quantified, measured for purity, and then subjected to microarray.
Sample_growth_protocol_ch1	All cell lines were growed with/ with no the HCV infection as in the reference.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	RMA by software Partek v6.5
Sample_platform_id	GPL570
Sample_contact_name	WeiPing,,Chen
Sample_contact_email	weipingChen@niddk.nih.gov
Sample_contact_phone	301-496-0175
Sample_contact_laboratory	Microarray Core Lab
Sample_contact_department	MCL
Sample_contact_institute	NIDDK/NIH
Sample_contact_address	Bldg 8, Room 1A11, NIDDK/NIH
Sample_contact_city	Bethesda
Sample_contact_state	MD
Sample_contact_zip/postal_code	20892
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1131nnn/GSM1131790/suppl/GSM1131790_01-27-10_JLiangJNT1_FQL_HG_U133Plus_2_JNT1.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1131nnn/GSM1131790/suppl/GSM1131790_01-27-10_JLiangJNT1_FQL_HG_U133Plus_2_JNT1.CHP.gz
Sample_series_id	GSE46528
Sample_data_row_count	54675

GSM1131791

GPL570

JNT2

JNT2_2

cell line: Huh7.5.1 treatment: treated with non-targeting control siRNA then infected with HCV

Sample_geo_accession	GSM1131791
Sample_status	Public on May 08 2013
Sample_submission_date	Apr 30 2013
Sample_last_update_date	May 08 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were treated with either non-targeting control siRNA or siRNA against IKKalpha at a final concentration of 50 nM. After 72 h, cells were either mock infected or infected with HCV JFH-1 strain with the M.O.I. of 0.5. At 48 h post-infection, cells were harvested, and total cellular RNA was extracted using a QIAGEN RNeasy mini kit. Total RNA was quantified, measured for purity, and then subjected to microarray.
Sample_growth_protocol_ch1	All cell lines were growed with/ with no the HCV infection as in the reference.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	RMA by software Partek v6.5
Sample_platform_id	GPL570
Sample_contact_name	WeiPing,,Chen
Sample_contact_email	weipingChen@niddk.nih.gov
Sample_contact_phone	301-496-0175
Sample_contact_laboratory	Microarray Core Lab
Sample_contact_department	MCL
Sample_contact_institute	NIDDK/NIH
Sample_contact_address	Bldg 8, Room 1A11, NIDDK/NIH
Sample_contact_city	Bethesda
Sample_contact_state	MD
Sample_contact_zip/postal_code	20892
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1131nnn/GSM1131791/suppl/GSM1131791_01-27-10_JLiangJNT2_FQL_HG_U133Plus_2_JNT2.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1131nnn/GSM1131791/suppl/GSM1131791_01-27-10_JLiangJNT2_FQL_HG_U133Plus_2_JNT2.CHP.gz
Sample_series_id	GSE46528
Sample_data_row_count	54675

GSM1131792

GPL570

NT1

NT1_1

cell line: Huh7.5.1 treatment: treated with non-targeting control siRNA

Sample_geo_accession	GSM1131792
Sample_status	Public on May 08 2013
Sample_submission_date	Apr 30 2013
Sample_last_update_date	May 08 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were treated with either non-targeting control siRNA or siRNA against IKKalpha at a final concentration of 50 nM. After 72 h, cells were either mock infected or infected with HCV JFH-1 strain with the M.O.I. of 0.5. At 48 h post-infection, cells were harvested, and total cellular RNA was extracted using a QIAGEN RNeasy mini kit. Total RNA was quantified, measured for purity, and then subjected to microarray.
Sample_growth_protocol_ch1	All cell lines were growed with/ with no the HCV infection as in the reference.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	RMA by software Partek v6.5
Sample_platform_id	GPL570
Sample_contact_name	WeiPing,,Chen
Sample_contact_email	weipingChen@niddk.nih.gov
Sample_contact_phone	301-496-0175
Sample_contact_laboratory	Microarray Core Lab
Sample_contact_department	MCL
Sample_contact_institute	NIDDK/NIH
Sample_contact_address	Bldg 8, Room 1A11, NIDDK/NIH
Sample_contact_city	Bethesda
Sample_contact_state	MD
Sample_contact_zip/postal_code	20892
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1131nnn/GSM1131792/suppl/GSM1131792_01-27-10_JLiangNT1_FQL_HG_U133Plus_2_NT1.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1131nnn/GSM1131792/suppl/GSM1131792_01-27-10_JLiangNT1_FQL_HG_U133Plus_2_NT1.CHP.gz
Sample_series_id	GSE46528
Sample_data_row_count	54675

GSM1131793

GPL570

NT2

NT2_2

cell line: Huh7.5.1 treatment: treated with non-targeting control siRNA

Sample_geo_accession	GSM1131793
Sample_status	Public on May 08 2013
Sample_submission_date	Apr 30 2013
Sample_last_update_date	May 08 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were treated with either non-targeting control siRNA or siRNA against IKKalpha at a final concentration of 50 nM. After 72 h, cells were either mock infected or infected with HCV JFH-1 strain with the M.O.I. of 0.5. At 48 h post-infection, cells were harvested, and total cellular RNA was extracted using a QIAGEN RNeasy mini kit. Total RNA was quantified, measured for purity, and then subjected to microarray.
Sample_growth_protocol_ch1	All cell lines were growed with/ with no the HCV infection as in the reference.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	RMA by software Partek v6.5
Sample_platform_id	GPL570
Sample_contact_name	WeiPing,,Chen
Sample_contact_email	weipingChen@niddk.nih.gov
Sample_contact_phone	301-496-0175
Sample_contact_laboratory	Microarray Core Lab
Sample_contact_department	MCL
Sample_contact_institute	NIDDK/NIH
Sample_contact_address	Bldg 8, Room 1A11, NIDDK/NIH
Sample_contact_city	Bethesda
Sample_contact_state	MD
Sample_contact_zip/postal_code	20892
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1131nnn/GSM1131793/suppl/GSM1131793_01-27-10_JLiangNT2_FQL_HG_U133Plus_2_NT2.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1131nnn/GSM1131793/suppl/GSM1131793_01-27-10_JLiangNT2_FQL_HG_U133Plus_2_NT2.CHP.gz
Sample_series_id	GSE46528
Sample_data_row_count	54675

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