Search results for the GEO ID: GSE46725 |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1136150 | GPL1261 |
|
E13.5 FL LSK HES1-GFP negative replicate 1
|
Lineage negative, cKit+, Sca1+ foetal liver cells from E13.5 embryo
|
gender: female
age: E13.5
genotype: Hes1 GFP/WT
cell type: Lineage negative, cKit+, Sca1+ foetal liver cells from E13.5 embryo
|
|
Sample_geo_accession | GSM1136150
| Sample_status | Public on Jul 01 2013
| Sample_submission_date | May 08 2013
| Sample_last_update_date | Jul 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | n/a
| Sample_growth_protocol_ch1 | n/a
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNEasy Plus mini Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
| Sample_hyb_protocol | 3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix)
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Genespring GX program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL1261
| Sample_contact_name | Camille,,Lobry
| Sample_contact_email | camille.lobry@nyumc.org
| Sample_contact_laboratory | Aifantis
| Sample_contact_department | Cancer Institute/Pathology
| Sample_contact_institute | NYULMC
| Sample_contact_address | 550 First Avenue
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1136nnn/GSM1136150/suppl/GSM1136150_FL_LSK_HES1-GFPneg_rep1.CEL.gz
| Sample_series_id | GSE46725
| Sample_series_id | GSE46726
| Sample_data_row_count | 45101
| |
|
GSM1136151 | GPL1261 |
|
E13.5 FL LSK HES1-GFP negative replicate 2
|
Lineage negative, cKit+, Sca1+ foetal liver cells from E13.5 embryo
|
gender: female
age: E13.5
genotype: Hes1 GFP/WT
cell type: Lineage negative, cKit+, Sca1+ foetal liver cells from E13.5 embryo
|
|
Sample_geo_accession | GSM1136151
| Sample_status | Public on Jul 01 2013
| Sample_submission_date | May 08 2013
| Sample_last_update_date | Jul 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | n/a
| Sample_growth_protocol_ch1 | n/a
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNEasy Plus mini Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
| Sample_hyb_protocol | 3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix)
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Genespring GX program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL1261
| Sample_contact_name | Camille,,Lobry
| Sample_contact_email | camille.lobry@nyumc.org
| Sample_contact_laboratory | Aifantis
| Sample_contact_department | Cancer Institute/Pathology
| Sample_contact_institute | NYULMC
| Sample_contact_address | 550 First Avenue
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1136nnn/GSM1136151/suppl/GSM1136151_FL_LSK_HES1-GFPneg_rep2.CEL.gz
| Sample_series_id | GSE46725
| Sample_series_id | GSE46726
| Sample_data_row_count | 45101
| |
|
GSM1136152 | GPL1261 |
|
E13.5 FL LSK HES1-GFP negative replicate 3
|
Lineage negative, cKit+, Sca1+ foetal liver cells from E13.5 embryo
|
gender: female
age: E13.5
genotype: Hes1 GFP/WT
cell type: Lineage negative, cKit+, Sca1+ foetal liver cells from E13.5 embryo
|
|
Sample_geo_accession | GSM1136152
| Sample_status | Public on Jul 01 2013
| Sample_submission_date | May 08 2013
| Sample_last_update_date | Jul 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | n/a
| Sample_growth_protocol_ch1 | n/a
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNEasy Plus mini Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
| Sample_hyb_protocol | 3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix)
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Genespring GX program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL1261
| Sample_contact_name | Camille,,Lobry
| Sample_contact_email | camille.lobry@nyumc.org
| Sample_contact_laboratory | Aifantis
| Sample_contact_department | Cancer Institute/Pathology
| Sample_contact_institute | NYULMC
| Sample_contact_address | 550 First Avenue
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1136nnn/GSM1136152/suppl/GSM1136152_FL_LSK_HES1-GFPpos_rep1.CEL.gz
| Sample_series_id | GSE46725
| Sample_series_id | GSE46726
| Sample_data_row_count | 45101
| |
|
GSM1136153 | GPL1261 |
|
E13.5 FL LSK HES1-GFP positive replicate 1
|
Lineage negative, cKit+, Sca1+ foetal liver cells from E13.5 embryo
|
gender: female
age: E13.5
genotype: Hes1 GFP/WT
cell type: Lineage negative, cKit+, Sca1+ foetal liver cells from E13.5 embryo
|
|
Sample_geo_accession | GSM1136153
| Sample_status | Public on Jul 01 2013
| Sample_submission_date | May 08 2013
| Sample_last_update_date | Jul 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | n/a
| Sample_growth_protocol_ch1 | n/a
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNEasy Plus mini Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
| Sample_hyb_protocol | 3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix)
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Genespring GX program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL1261
| Sample_contact_name | Camille,,Lobry
| Sample_contact_email | camille.lobry@nyumc.org
| Sample_contact_laboratory | Aifantis
| Sample_contact_department | Cancer Institute/Pathology
| Sample_contact_institute | NYULMC
| Sample_contact_address | 550 First Avenue
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1136nnn/GSM1136153/suppl/GSM1136153_FL_LSK_HES1-GFPpos_rep2.CEL.gz
| Sample_series_id | GSE46725
| Sample_series_id | GSE46726
| Sample_data_row_count | 45101
| |
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