Sample_geo_accession | GSM1138528
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Sample_status | Public on May 10 2013
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Sample_submission_date | May 09 2013
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Sample_last_update_date | May 10 2013
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Sample_type | RNA
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Sample_channel_count | 1
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Sample_organism_ch1 | Mus musculus
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Sample_taxid_ch1 | 10090
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Sample_treatment_protocol_ch1 | Qiazol extraction of total RNA was performed according to the manufacturer's instructions.
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Sample_growth_protocol_ch1 | Plugged day was counted embryonic day 0.5. In E10.5, embryos were extracted total RNA.
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Sample_molecule_ch1 | total RNA
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Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy mini kit according to the manufacturer's instructions.
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Sample_label_ch1 | Biotin
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Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA .
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Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
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Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip 3000 Scanner.
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Sample_data_processing | The data were checked with Affymetrix GeneChip Command Console Software, analyzed with Expression Console using default analysis settings and global scaling as normalization method.
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Sample_platform_id | GPL1261
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Sample_contact_name | Seiji,,Yamamoto
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Sample_contact_email | seiyama.flyfishing@gmail.com
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Sample_contact_phone | +81-76-434-2281
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Sample_contact_department | Department of Pathology
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Sample_contact_institute | University of Toyama
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Sample_contact_address | 2630 Sugitani
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Sample_contact_city | Toyama
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Sample_contact_state | Toayma
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Sample_contact_zip/postal_code | 930-0194
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Sample_contact_country | Japan
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Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1138nnn/GSM1138528/suppl/GSM1138528_PF090512_01.CEL.gz
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Sample_series_id | GSE46800
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Sample_data_row_count | 45101
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