Search results for the GEO ID: GSE46871  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1139478 | GPL1261 |
|
Hippocampus, Tg2576-APPSwe mice, 18 month-old, with high Abeta plaque load-rep1
|
Hippocampi isolated from 18 month-old Tg2576 APPSwe-transgenic mice with high Abeta plaque load
|
strain: C57BL/6
tissue: Hippocampi
Sex: male
age: 18 months old
genotype/variations: Tg2576 APPSwe-transgenic mice
|
Gene expression data of hippocampi isolated from 18 month-old untreated Tg2576 (APPSwe-transgenic) AD mice with high Abeta plaque load
|
| Sample_geo_accession | GSM1139478
| | Sample_status | Public on May 14 2013
| | Sample_submission_date | May 13 2013
| | Sample_last_update_date | May 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The study was performed with hippocampi isolated from three groups of mice, i.e. (i) untreated 18 month-old Tg2576 (APPSwe-transgenic) AD mice with high Abeta plaque load (APP-18 mo), (ii) 18 month-old Tg2576 (APPSwe-transgenic) AD mice treated for 6 months with the brain-penetrating ACE inhibitor, captopril (20mg/kg/day in drinking water; APP-18mo-ACEI), and (iii) untreated 12 month-old Tg2576 (APPSwe-transgenic) AD mice with low Abeta plaque load (APP-12mo).
| | Sample_growth_protocol_ch1 | The study was performed with 18 month-old Tg2576 (APPSwe-transgenic) AD mice treated for six months without or with the brain-penetrating ACE inhibitor, captopril. Mice had free access to food and water, and were fed a standard rodent chow. The treatment group of Tg2576 mice received the centrally active ACE inhibitor, captopril (20 mg/kg/day supplied in drinking water) for 6 months. The control group of Tg2576 mice received drinking water without captopril. At an age of 18 months, hippocampi were isolated from Tg2576 mice treated without or with captopril for six months. As an additional control group, hippocampi were isolated from 12 month-old untreated Tg2576 mice with low Abeta plaque load, reflecting the time point when treatment with captopril was initiated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total hippocampal RNA was isolated with the RNeasy Mini kit according to the manufacturer`s instructions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev.3).
| | Sample_hyb_protocol | For hybridization, 15 µg of fragmented, biotin-labeled cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 600.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ursula,,Quitterer
| | Sample_contact_email | ursula.quitterer@pharma.ethz.ch
| | Sample_contact_phone | 0041 44 635 6001
| | Sample_contact_fax | 0041 44 635 6881
| | Sample_contact_department | Molecular Pharmacology
| | Sample_contact_institute | Swiss Federal Institute of Technology
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | CH-8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1139nnn/GSM1139478/suppl/GSM1139478_APP-18mo-1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1139nnn/GSM1139478/suppl/GSM1139478_APP-18mo-1.CHP.gz
| | Sample_series_id | GSE46871
| | Sample_data_row_count | 45101
| | |
|
GSM1139479 | GPL1261 |
|
Hippocampus, Tg2576-APPSwe mice, 18 month-old, with high Abeta plaque load-rep2
|
Hippocampi isolated from 18 month-old Tg2576 APPSwe-transgenic mice with high Abeta plaque load
|
strain: C57BL/6
tissue: Hippocampi
Sex: male
age: 18 months old
genotype/variations: Tg2576 APPSwe-transgenic mice
|
Gene expression data of hippocampi isolated from 18 month-old untreated Tg2576 (APPSwe-transgenic) AD mice with high Abeta plaque load
|
| Sample_geo_accession | GSM1139479
| | Sample_status | Public on May 14 2013
| | Sample_submission_date | May 13 2013
| | Sample_last_update_date | May 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The study was performed with hippocampi isolated from three groups of mice, i.e. (i) untreated 18 month-old Tg2576 (APPSwe-transgenic) AD mice with high Abeta plaque load (APP-18 mo), (ii) 18 month-old Tg2576 (APPSwe-transgenic) AD mice treated for 6 months with the brain-penetrating ACE inhibitor, captopril (20mg/kg/day in drinking water; APP-18mo-ACEI), and (iii) untreated 12 month-old Tg2576 (APPSwe-transgenic) AD mice with low Abeta plaque load (APP-12mo).
| | Sample_growth_protocol_ch1 | The study was performed with 18 month-old Tg2576 (APPSwe-transgenic) AD mice treated for six months without or with the brain-penetrating ACE inhibitor, captopril. Mice had free access to food and water, and were fed a standard rodent chow. The treatment group of Tg2576 mice received the centrally active ACE inhibitor, captopril (20 mg/kg/day supplied in drinking water) for 6 months. The control group of Tg2576 mice received drinking water without captopril. At an age of 18 months, hippocampi were isolated from Tg2576 mice treated without or with captopril for six months. As an additional control group, hippocampi were isolated from 12 month-old untreated Tg2576 mice with low Abeta plaque load, reflecting the time point when treatment with captopril was initiated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total hippocampal RNA was isolated with the RNeasy Mini kit according to the manufacturer`s instructions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev.3).
| | Sample_hyb_protocol | For hybridization, 15 µg of fragmented, biotin-labeled cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 600.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ursula,,Quitterer
| | Sample_contact_email | ursula.quitterer@pharma.ethz.ch
| | Sample_contact_phone | 0041 44 635 6001
| | Sample_contact_fax | 0041 44 635 6881
| | Sample_contact_department | Molecular Pharmacology
| | Sample_contact_institute | Swiss Federal Institute of Technology
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | CH-8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1139nnn/GSM1139479/suppl/GSM1139479_APP-18mo-2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1139nnn/GSM1139479/suppl/GSM1139479_APP-18mo-2.CHP.gz
| | Sample_series_id | GSE46871
| | Sample_data_row_count | 45101
| | |
|
GSM1139480 | GPL1261 |
|
Hippocampus, Tg2576-APPSwe mice, 18 month-old, ACE inhibitor-treated-rep1
|
Hippocampi isolated from 18 month-old Tg2576 APPSwe-transgenic mice treated for 6 months with the brain-penetrating ACE inhibitor captopril
|
strain: C57BL/6
tissue: Hippocampi
Sex: male
age: 18 months old
genotype/variations: Tg2576 APPSwe-transgenic mice
|
Gene expression data of hippocampi isolated from 18 month-old Tg2576 (APPSwe-transgenic) AD mice treated for 6 months with the brain-penetrating ACE inhibitor captopril
|
| Sample_geo_accession | GSM1139480
| | Sample_status | Public on May 14 2013
| | Sample_submission_date | May 13 2013
| | Sample_last_update_date | May 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The study was performed with hippocampi isolated from three groups of mice, i.e. (i) untreated 18 month-old Tg2576 (APPSwe-transgenic) AD mice with high Abeta plaque load (APP-18 mo), (ii) 18 month-old Tg2576 (APPSwe-transgenic) AD mice treated for 6 months with the brain-penetrating ACE inhibitor, captopril (20mg/kg/day in drinking water; APP-18mo-ACEI), and (iii) untreated 12 month-old Tg2576 (APPSwe-transgenic) AD mice with low Abeta plaque load (APP-12mo).
| | Sample_growth_protocol_ch1 | The study was performed with 18 month-old Tg2576 (APPSwe-transgenic) AD mice treated for six months without or with the brain-penetrating ACE inhibitor, captopril. Mice had free access to food and water, and were fed a standard rodent chow. The treatment group of Tg2576 mice received the centrally active ACE inhibitor, captopril (20 mg/kg/day supplied in drinking water) for 6 months. The control group of Tg2576 mice received drinking water without captopril. At an age of 18 months, hippocampi were isolated from Tg2576 mice treated without or with captopril for six months. As an additional control group, hippocampi were isolated from 12 month-old untreated Tg2576 mice with low Abeta plaque load, reflecting the time point when treatment with captopril was initiated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total hippocampal RNA was isolated with the RNeasy Mini kit according to the manufacturer`s instructions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev.3).
| | Sample_hyb_protocol | For hybridization, 15 µg of fragmented, biotin-labeled cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 600.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ursula,,Quitterer
| | Sample_contact_email | ursula.quitterer@pharma.ethz.ch
| | Sample_contact_phone | 0041 44 635 6001
| | Sample_contact_fax | 0041 44 635 6881
| | Sample_contact_department | Molecular Pharmacology
| | Sample_contact_institute | Swiss Federal Institute of Technology
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | CH-8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1139nnn/GSM1139480/suppl/GSM1139480_APP-18mo-ACEI-1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1139nnn/GSM1139480/suppl/GSM1139480_APP-18mo-ACEI-1.CHP.gz
| | Sample_series_id | GSE46871
| | Sample_data_row_count | 45101
| | |
|
GSM1139481 | GPL1261 |
|
Hippocampus, Tg2576-APPSwe mice, 18 month-old, ACE inhibitor-treated-rep2
|
Hippocampi isolated from 18 month-old Tg2576 APPSwe-transgenic mice treated for 6 months with the brain-penetrating ACE inhibitor captopril
|
strain: C57BL/6
tissue: Hippocampi
Sex: male
age: 18 months old
genotype/variations: Tg2576 APPSwe-transgenic mice
|
Gene expression data of hippocampi isolated from 18 month-old Tg2576 (APPSwe-transgenic) AD mice treated for 6 months with the brain-penetrating ACE inhibitor captopril
|
| Sample_geo_accession | GSM1139481
| | Sample_status | Public on May 14 2013
| | Sample_submission_date | May 13 2013
| | Sample_last_update_date | May 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The study was performed with hippocampi isolated from three groups of mice, i.e. (i) untreated 18 month-old Tg2576 (APPSwe-transgenic) AD mice with high Abeta plaque load (APP-18 mo), (ii) 18 month-old Tg2576 (APPSwe-transgenic) AD mice treated for 6 months with the brain-penetrating ACE inhibitor, captopril (20mg/kg/day in drinking water; APP-18mo-ACEI), and (iii) untreated 12 month-old Tg2576 (APPSwe-transgenic) AD mice with low Abeta plaque load (APP-12mo).
| | Sample_growth_protocol_ch1 | The study was performed with 18 month-old Tg2576 (APPSwe-transgenic) AD mice treated for six months without or with the brain-penetrating ACE inhibitor, captopril. Mice had free access to food and water, and were fed a standard rodent chow. The treatment group of Tg2576 mice received the centrally active ACE inhibitor, captopril (20 mg/kg/day supplied in drinking water) for 6 months. The control group of Tg2576 mice received drinking water without captopril. At an age of 18 months, hippocampi were isolated from Tg2576 mice treated without or with captopril for six months. As an additional control group, hippocampi were isolated from 12 month-old untreated Tg2576 mice with low Abeta plaque load, reflecting the time point when treatment with captopril was initiated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total hippocampal RNA was isolated with the RNeasy Mini kit according to the manufacturer`s instructions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev.3).
| | Sample_hyb_protocol | For hybridization, 15 µg of fragmented, biotin-labeled cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 600.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ursula,,Quitterer
| | Sample_contact_email | ursula.quitterer@pharma.ethz.ch
| | Sample_contact_phone | 0041 44 635 6001
| | Sample_contact_fax | 0041 44 635 6881
| | Sample_contact_department | Molecular Pharmacology
| | Sample_contact_institute | Swiss Federal Institute of Technology
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | CH-8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1139nnn/GSM1139481/suppl/GSM1139481_APP-18mo-ACEI-2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1139nnn/GSM1139481/suppl/GSM1139481_APP-18mo-ACEI-2.CHP.gz
| | Sample_series_id | GSE46871
| | Sample_data_row_count | 45101
| | |
|
GSM1139482 | GPL1261 |
|
Hippocampus, Tg2576-APPSwe mice, 12 month-old, with low Abeta plaque load-rep1
|
Hippocampi isolated from 12 month-old Tg2576 APPSwe-transgenic mice with low Abeta plaque load
|
strain: C57BL/6
tissue: Hippocampi
Sex: male
age: 12 months old
genotype/variations: Tg2576 APPSwe-transgenic mice
|
Gene expression data of hippocampi isolated from 12 month-old untreated Tg2576 (APPSwe-transgenic) AD mice with low Abeta plaque load
|
| Sample_geo_accession | GSM1139482
| | Sample_status | Public on May 14 2013
| | Sample_submission_date | May 13 2013
| | Sample_last_update_date | May 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The study was performed with hippocampi isolated from three groups of mice, i.e. (i) untreated 18 month-old Tg2576 (APPSwe-transgenic) AD mice with high Abeta plaque load (APP-18 mo), (ii) 18 month-old Tg2576 (APPSwe-transgenic) AD mice treated for 6 months with the brain-penetrating ACE inhibitor, captopril (20mg/kg/day in drinking water; APP-18mo-ACEI), and (iii) untreated 12 month-old Tg2576 (APPSwe-transgenic) AD mice with low Abeta plaque load (APP-12mo).
| | Sample_growth_protocol_ch1 | The study was performed with 18 month-old Tg2576 (APPSwe-transgenic) AD mice treated for six months without or with the brain-penetrating ACE inhibitor, captopril. Mice had free access to food and water, and were fed a standard rodent chow. The treatment group of Tg2576 mice received the centrally active ACE inhibitor, captopril (20 mg/kg/day supplied in drinking water) for 6 months. The control group of Tg2576 mice received drinking water without captopril. At an age of 18 months, hippocampi were isolated from Tg2576 mice treated without or with captopril for six months. As an additional control group, hippocampi were isolated from 12 month-old untreated Tg2576 mice with low Abeta plaque load, reflecting the time point when treatment with captopril was initiated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total hippocampal RNA was isolated with the RNeasy Mini kit according to the manufacturer`s instructions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev.3).
| | Sample_hyb_protocol | For hybridization, 15 µg of fragmented, biotin-labeled cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 600.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ursula,,Quitterer
| | Sample_contact_email | ursula.quitterer@pharma.ethz.ch
| | Sample_contact_phone | 0041 44 635 6001
| | Sample_contact_fax | 0041 44 635 6881
| | Sample_contact_department | Molecular Pharmacology
| | Sample_contact_institute | Swiss Federal Institute of Technology
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | CH-8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1139nnn/GSM1139482/suppl/GSM1139482_APP-12mo-1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1139nnn/GSM1139482/suppl/GSM1139482_APP-12mo-1.CHP.gz
| | Sample_series_id | GSE46871
| | Sample_data_row_count | 45101
| | |
|
GSM1139483 | GPL1261 |
|
Hippocampus, Tg2576-APPSwe mice, 12 month-old, with low Abeta plaque load-rep2
|
Hippocampi isolated from 12 month-old Tg2576 APPSwe-transgenic mice with low Abeta plaque load
|
strain: C57BL/6
tissue: Hippocampi
Sex: male
age: 12 months old
genotype/variations: Tg2576 APPSwe-transgenic mice
|
Gene expression data of hippocampi isolated from 12 month-old untreated Tg2576 (APPSwe-transgenic) AD mice with low Abeta plaque load
|
| Sample_geo_accession | GSM1139483
| | Sample_status | Public on May 14 2013
| | Sample_submission_date | May 13 2013
| | Sample_last_update_date | May 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The study was performed with hippocampi isolated from three groups of mice, i.e. (i) untreated 18 month-old Tg2576 (APPSwe-transgenic) AD mice with high Abeta plaque load (APP-18 mo), (ii) 18 month-old Tg2576 (APPSwe-transgenic) AD mice treated for 6 months with the brain-penetrating ACE inhibitor, captopril (20mg/kg/day in drinking water; APP-18mo-ACEI), and (iii) untreated 12 month-old Tg2576 (APPSwe-transgenic) AD mice with low Abeta plaque load (APP-12mo).
| | Sample_growth_protocol_ch1 | The study was performed with 18 month-old Tg2576 (APPSwe-transgenic) AD mice treated for six months without or with the brain-penetrating ACE inhibitor, captopril. Mice had free access to food and water, and were fed a standard rodent chow. The treatment group of Tg2576 mice received the centrally active ACE inhibitor, captopril (20 mg/kg/day supplied in drinking water) for 6 months. The control group of Tg2576 mice received drinking water without captopril. At an age of 18 months, hippocampi were isolated from Tg2576 mice treated without or with captopril for six months. As an additional control group, hippocampi were isolated from 12 month-old untreated Tg2576 mice with low Abeta plaque load, reflecting the time point when treatment with captopril was initiated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total hippocampal RNA was isolated with the RNeasy Mini kit according to the manufacturer`s instructions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev.3).
| | Sample_hyb_protocol | For hybridization, 15 µg of fragmented, biotin-labeled cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 600.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ursula,,Quitterer
| | Sample_contact_email | ursula.quitterer@pharma.ethz.ch
| | Sample_contact_phone | 0041 44 635 6001
| | Sample_contact_fax | 0041 44 635 6881
| | Sample_contact_department | Molecular Pharmacology
| | Sample_contact_institute | Swiss Federal Institute of Technology
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | CH-8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1139nnn/GSM1139483/suppl/GSM1139483_APP-12mo-2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1139nnn/GSM1139483/suppl/GSM1139483_APP-12mo-2.CHP.gz
| | Sample_series_id | GSE46871
| | Sample_data_row_count | 45101
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