Search results for the GEO ID: GSE46873 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1139496 | GPL570 |
|
Raji_NT_rep1
|
Raji_non transduced
|
cell line: Raji
cell type: Raji Burkitt lymphoma B cells
transduced with: none (non-transduced control)
|
Gene expression data from Raji cells non transduced
|
Sample_geo_accession | GSM1139496
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | May 13 2013
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Stable cell lines have been established using lentiviral transduction of Raji cells with either a control (non-targeting) shRNA sequence or CYCLON shRNA constructs under puromycin selection.
| Sample_growth_protocol_ch1 | Raji cells were cultured in RPMI supplemented with 20 % fœtal bovine serum, sodium pyruvate, non esssential amino acids and penicilllin/streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared using the kit GeneChip IVT express according to manufacturer’s instructions.
| Sample_hyb_protocol | cRNA was hybridized to HG U133 Plus 2.0 microarrays according to manufacturer’s instructions.
| Sample_scan_protocol | Fluorescence intensities were quantified using the GCOS 1.2 software
| Sample_data_processing | The data were analyzed with GeneSpring software (v.11.0, Agilent) using default analysis settings and RMA normalization (with no baseline transormation).
| Sample_platform_id | GPL570
| Sample_contact_name | Anouk,,Emadali
| Sample_contact_email | anouk.emadali@ujf-grenoble.fr
| Sample_contact_institute | Institut Albert Bonniot
| Sample_contact_address | BP170
| Sample_contact_city | Grenoble
| Sample_contact_zip/postal_code | 38042
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1139nnn/GSM1139496/suppl/GSM1139496_raji_NT_1_20110310_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE46873
| Sample_data_row_count | 54675
| |
|
GSM1139497 | GPL570 |
|
Raji_NT_rep2
|
Raji_non transduced
|
cell line: Raji
cell type: Raji Burkitt lymphoma B cells
transduced with: none (non-transduced control)
|
Gene expression data from Raji cells non transduced
|
Sample_geo_accession | GSM1139497
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | May 13 2013
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Stable cell lines have been established using lentiviral transduction of Raji cells with either a control (non-targeting) shRNA sequence or CYCLON shRNA constructs under puromycin selection.
| Sample_growth_protocol_ch1 | Raji cells were cultured in RPMI supplemented with 20 % fœtal bovine serum, sodium pyruvate, non esssential amino acids and penicilllin/streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared using the kit GeneChip IVT express according to manufacturer’s instructions.
| Sample_hyb_protocol | cRNA was hybridized to HG U133 Plus 2.0 microarrays according to manufacturer’s instructions.
| Sample_scan_protocol | Fluorescence intensities were quantified using the GCOS 1.2 software
| Sample_data_processing | The data were analyzed with GeneSpring software (v.11.0, Agilent) using default analysis settings and RMA normalization (with no baseline transormation).
| Sample_platform_id | GPL570
| Sample_contact_name | Anouk,,Emadali
| Sample_contact_email | anouk.emadali@ujf-grenoble.fr
| Sample_contact_institute | Institut Albert Bonniot
| Sample_contact_address | BP170
| Sample_contact_city | Grenoble
| Sample_contact_zip/postal_code | 38042
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1139nnn/GSM1139497/suppl/GSM1139497_raji_NT_2_20110310_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE46873
| Sample_data_row_count | 54675
| |
|
GSM1139498 | GPL570 |
|
Raji_NT_rep3
|
Raji_non transduced
|
cell line: Raji
cell type: Raji Burkitt lymphoma B cells
transduced with: none (non-transduced control)
|
Gene expression data from Raji cells non transduced
|
Sample_geo_accession | GSM1139498
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | May 13 2013
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Stable cell lines have been established using lentiviral transduction of Raji cells with either a control (non-targeting) shRNA sequence or CYCLON shRNA constructs under puromycin selection.
| Sample_growth_protocol_ch1 | Raji cells were cultured in RPMI supplemented with 20 % fœtal bovine serum, sodium pyruvate, non esssential amino acids and penicilllin/streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared using the kit GeneChip IVT express according to manufacturer’s instructions.
| Sample_hyb_protocol | cRNA was hybridized to HG U133 Plus 2.0 microarrays according to manufacturer’s instructions.
| Sample_scan_protocol | Fluorescence intensities were quantified using the GCOS 1.2 software
| Sample_data_processing | The data were analyzed with GeneSpring software (v.11.0, Agilent) using default analysis settings and RMA normalization (with no baseline transormation).
| Sample_platform_id | GPL570
| Sample_contact_name | Anouk,,Emadali
| Sample_contact_email | anouk.emadali@ujf-grenoble.fr
| Sample_contact_institute | Institut Albert Bonniot
| Sample_contact_address | BP170
| Sample_contact_city | Grenoble
| Sample_contact_zip/postal_code | 38042
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1139nnn/GSM1139498/suppl/GSM1139498_raji_NT_3_20110310_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE46873
| Sample_data_row_count | 54675
| |
|
GSM1139499 | GPL570 |
|
Raji_NT_rep4
|
Raji_non transduced
|
cell line: Raji
cell type: Raji Burkitt lymphoma B cells
transduced with: none (non-transduced control)
|
Gene expression data from Raji cells non transduced
|
Sample_geo_accession | GSM1139499
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | May 13 2013
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Stable cell lines have been established using lentiviral transduction of Raji cells with either a control (non-targeting) shRNA sequence or CYCLON shRNA constructs under puromycin selection.
| Sample_growth_protocol_ch1 | Raji cells were cultured in RPMI supplemented with 20 % fœtal bovine serum, sodium pyruvate, non esssential amino acids and penicilllin/streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared using the kit GeneChip IVT express according to manufacturer’s instructions.
| Sample_hyb_protocol | cRNA was hybridized to HG U133 Plus 2.0 microarrays according to manufacturer’s instructions.
| Sample_scan_protocol | Fluorescence intensities were quantified using the GCOS 1.2 software
| Sample_data_processing | The data were analyzed with GeneSpring software (v.11.0, Agilent) using default analysis settings and RMA normalization (with no baseline transormation).
| Sample_platform_id | GPL570
| Sample_contact_name | Anouk,,Emadali
| Sample_contact_email | anouk.emadali@ujf-grenoble.fr
| Sample_contact_institute | Institut Albert Bonniot
| Sample_contact_address | BP170
| Sample_contact_city | Grenoble
| Sample_contact_zip/postal_code | 38042
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1139nnn/GSM1139499/suppl/GSM1139499_raji_NT_4_20110310_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE46873
| Sample_data_row_count | 54675
| |
|
GSM1139500 | GPL570 |
|
Raji_shCtrl_rep1
|
Raji_transduced with a non targeting (Ctrl) sh RNA
|
cell line: Raji
cell type: Raji Burkitt lymphoma B cells
transduced with: control (non-targeting) shRNA
|
Gene expression data from Raji cells transduced with a non targeting (Ctrl) sh RNA
|
Sample_geo_accession | GSM1139500
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | May 13 2013
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Stable cell lines have been established using lentiviral transduction of Raji cells with either a control (non-targeting) shRNA sequence or CYCLON shRNA constructs under puromycin selection.
| Sample_growth_protocol_ch1 | Raji cells were cultured in RPMI supplemented with 20 % fœtal bovine serum, sodium pyruvate, non esssential amino acids and penicilllin/streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared using the kit GeneChip IVT express according to manufacturer’s instructions.
| Sample_hyb_protocol | cRNA was hybridized to HG U133 Plus 2.0 microarrays according to manufacturer’s instructions.
| Sample_scan_protocol | Fluorescence intensities were quantified using the GCOS 1.2 software
| Sample_data_processing | The data were analyzed with GeneSpring software (v.11.0, Agilent) using default analysis settings and RMA normalization (with no baseline transormation).
| Sample_platform_id | GPL570
| Sample_contact_name | Anouk,,Emadali
| Sample_contact_email | anouk.emadali@ujf-grenoble.fr
| Sample_contact_institute | Institut Albert Bonniot
| Sample_contact_address | BP170
| Sample_contact_city | Grenoble
| Sample_contact_zip/postal_code | 38042
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1139nnn/GSM1139500/suppl/GSM1139500_raji_shctrl_MOl_3-8_1_20110310_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE46873
| Sample_data_row_count | 54675
| |
|
GSM1139501 | GPL570 |
|
Raji_shCtrl_rep2
|
Raji_transduced with a non targeting (Ctrl) sh RNA
|
cell line: Raji
cell type: Raji Burkitt lymphoma B cells
transduced with: control (non-targeting) shRNA
|
Gene expression data from Raji cells transduced with a non targeting (Ctrl) sh RNA
|
Sample_geo_accession | GSM1139501
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | May 13 2013
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Stable cell lines have been established using lentiviral transduction of Raji cells with either a control (non-targeting) shRNA sequence or CYCLON shRNA constructs under puromycin selection.
| Sample_growth_protocol_ch1 | Raji cells were cultured in RPMI supplemented with 20 % fœtal bovine serum, sodium pyruvate, non esssential amino acids and penicilllin/streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared using the kit GeneChip IVT express according to manufacturer’s instructions.
| Sample_hyb_protocol | cRNA was hybridized to HG U133 Plus 2.0 microarrays according to manufacturer’s instructions.
| Sample_scan_protocol | Fluorescence intensities were quantified using the GCOS 1.2 software
| Sample_data_processing | The data were analyzed with GeneSpring software (v.11.0, Agilent) using default analysis settings and RMA normalization (with no baseline transormation).
| Sample_platform_id | GPL570
| Sample_contact_name | Anouk,,Emadali
| Sample_contact_email | anouk.emadali@ujf-grenoble.fr
| Sample_contact_institute | Institut Albert Bonniot
| Sample_contact_address | BP170
| Sample_contact_city | Grenoble
| Sample_contact_zip/postal_code | 38042
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1139nnn/GSM1139501/suppl/GSM1139501_raji_shctrl_MOl_3-8_2_20110310_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE46873
| Sample_data_row_count | 54675
| |
|
GSM1139502 | GPL570 |
|
Raji_shCtrl_rep3
|
Raji_transduced with a non targeting (Ctrl) sh RNA
|
cell line: Raji
cell type: Raji Burkitt lymphoma B cells
transduced with: control (non-targeting) shRNA
|
Gene expression data from Raji cells transduced with a non targeting (Ctrl) sh RNA
|
Sample_geo_accession | GSM1139502
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | May 13 2013
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Stable cell lines have been established using lentiviral transduction of Raji cells with either a control (non-targeting) shRNA sequence or CYCLON shRNA constructs under puromycin selection.
| Sample_growth_protocol_ch1 | Raji cells were cultured in RPMI supplemented with 20 % fœtal bovine serum, sodium pyruvate, non esssential amino acids and penicilllin/streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared using the kit GeneChip IVT express according to manufacturer’s instructions.
| Sample_hyb_protocol | cRNA was hybridized to HG U133 Plus 2.0 microarrays according to manufacturer’s instructions.
| Sample_scan_protocol | Fluorescence intensities were quantified using the GCOS 1.2 software
| Sample_data_processing | The data were analyzed with GeneSpring software (v.11.0, Agilent) using default analysis settings and RMA normalization (with no baseline transormation).
| Sample_platform_id | GPL570
| Sample_contact_name | Anouk,,Emadali
| Sample_contact_email | anouk.emadali@ujf-grenoble.fr
| Sample_contact_institute | Institut Albert Bonniot
| Sample_contact_address | BP170
| Sample_contact_city | Grenoble
| Sample_contact_zip/postal_code | 38042
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1139nnn/GSM1139502/suppl/GSM1139502_raji_shctrl_MOl_3-8_3_20110310_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE46873
| Sample_data_row_count | 54675
| |
|
GSM1139503 | GPL570 |
|
Raji_shCtrl_rep4
|
Raji_transduced with a non targeting (Ctrl) sh RNA
|
cell line: Raji
cell type: Raji Burkitt lymphoma B cells
transduced with: control (non-targeting) shRNA
|
Gene expression data from Raji cells transduced with a non targeting (Ctrl) sh RNA
|
Sample_geo_accession | GSM1139503
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | May 13 2013
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Stable cell lines have been established using lentiviral transduction of Raji cells with either a control (non-targeting) shRNA sequence or CYCLON shRNA constructs under puromycin selection.
| Sample_growth_protocol_ch1 | Raji cells were cultured in RPMI supplemented with 20 % fœtal bovine serum, sodium pyruvate, non esssential amino acids and penicilllin/streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared using the kit GeneChip IVT express according to manufacturer’s instructions.
| Sample_hyb_protocol | cRNA was hybridized to HG U133 Plus 2.0 microarrays according to manufacturer’s instructions.
| Sample_scan_protocol | Fluorescence intensities were quantified using the GCOS 1.2 software
| Sample_data_processing | The data were analyzed with GeneSpring software (v.11.0, Agilent) using default analysis settings and RMA normalization (with no baseline transormation).
| Sample_platform_id | GPL570
| Sample_contact_name | Anouk,,Emadali
| Sample_contact_email | anouk.emadali@ujf-grenoble.fr
| Sample_contact_institute | Institut Albert Bonniot
| Sample_contact_address | BP170
| Sample_contact_city | Grenoble
| Sample_contact_zip/postal_code | 38042
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1139nnn/GSM1139503/suppl/GSM1139503_raji_shctrl_MOl_3-8_4_20110310_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE46873
| Sample_data_row_count | 54675
| |
|
GSM1139504 | GPL570 |
|
Raji_shCyclon_rep1
|
Raji_transduced with a sh RNA targeting CYCLON
|
cell line: Raji
cell type: Raji Burkitt lymphoma B cells
transduced with: CYCLON shRNA constructs
|
Gene expression data from Raji cells transduced with a sh RNA targeting CYCLON
|
Sample_geo_accession | GSM1139504
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | May 13 2013
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Stable cell lines have been established using lentiviral transduction of Raji cells with either a control (non-targeting) shRNA sequence or CYCLON shRNA constructs under puromycin selection.
| Sample_growth_protocol_ch1 | Raji cells were cultured in RPMI supplemented with 20 % fœtal bovine serum, sodium pyruvate, non esssential amino acids and penicilllin/streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared using the kit GeneChip IVT express according to manufacturer’s instructions.
| Sample_hyb_protocol | cRNA was hybridized to HG U133 Plus 2.0 microarrays according to manufacturer’s instructions.
| Sample_scan_protocol | Fluorescence intensities were quantified using the GCOS 1.2 software
| Sample_data_processing | The data were analyzed with GeneSpring software (v.11.0, Agilent) using default analysis settings and RMA normalization (with no baseline transormation).
| Sample_platform_id | GPL570
| Sample_contact_name | Anouk,,Emadali
| Sample_contact_email | anouk.emadali@ujf-grenoble.fr
| Sample_contact_institute | Institut Albert Bonniot
| Sample_contact_address | BP170
| Sample_contact_city | Grenoble
| Sample_contact_zip/postal_code | 38042
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1139nnn/GSM1139504/suppl/GSM1139504_raji_shcyclon_MOl_3-8_1_20110311_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE46873
| Sample_data_row_count | 54675
| |
|
GSM1139505 | GPL570 |
|
Raji_shCyclon_rep2
|
Raji_transduced with a sh RNA targeting CYCLON
|
cell line: Raji
cell type: Raji Burkitt lymphoma B cells
transduced with: CYCLON shRNA constructs
|
Gene expression data from Raji cells transduced with a sh RNA targeting CYCLON
|
Sample_geo_accession | GSM1139505
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | May 13 2013
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Stable cell lines have been established using lentiviral transduction of Raji cells with either a control (non-targeting) shRNA sequence or CYCLON shRNA constructs under puromycin selection.
| Sample_growth_protocol_ch1 | Raji cells were cultured in RPMI supplemented with 20 % fœtal bovine serum, sodium pyruvate, non esssential amino acids and penicilllin/streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared using the kit GeneChip IVT express according to manufacturer’s instructions.
| Sample_hyb_protocol | cRNA was hybridized to HG U133 Plus 2.0 microarrays according to manufacturer’s instructions.
| Sample_scan_protocol | Fluorescence intensities were quantified using the GCOS 1.2 software
| Sample_data_processing | The data were analyzed with GeneSpring software (v.11.0, Agilent) using default analysis settings and RMA normalization (with no baseline transormation).
| Sample_platform_id | GPL570
| Sample_contact_name | Anouk,,Emadali
| Sample_contact_email | anouk.emadali@ujf-grenoble.fr
| Sample_contact_institute | Institut Albert Bonniot
| Sample_contact_address | BP170
| Sample_contact_city | Grenoble
| Sample_contact_zip/postal_code | 38042
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1139nnn/GSM1139505/suppl/GSM1139505_raji_shcyclon_MOl_3-8_2_20110311_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE46873
| Sample_data_row_count | 54675
| |
|
GSM1139506 | GPL570 |
|
Raji_shCyclon_rep3
|
Raji_transduced with a sh RNA targeting CYCLON
|
cell line: Raji
cell type: Raji Burkitt lymphoma B cells
transduced with: CYCLON shRNA constructs
|
Gene expression data from Raji cells transduced with a sh RNA targeting CYCLON
|
Sample_geo_accession | GSM1139506
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | May 13 2013
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Stable cell lines have been established using lentiviral transduction of Raji cells with either a control (non-targeting) shRNA sequence or CYCLON shRNA constructs under puromycin selection.
| Sample_growth_protocol_ch1 | Raji cells were cultured in RPMI supplemented with 20 % fœtal bovine serum, sodium pyruvate, non esssential amino acids and penicilllin/streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared using the kit GeneChip IVT express according to manufacturer’s instructions.
| Sample_hyb_protocol | cRNA was hybridized to HG U133 Plus 2.0 microarrays according to manufacturer’s instructions.
| Sample_scan_protocol | Fluorescence intensities were quantified using the GCOS 1.2 software
| Sample_data_processing | The data were analyzed with GeneSpring software (v.11.0, Agilent) using default analysis settings and RMA normalization (with no baseline transormation).
| Sample_platform_id | GPL570
| Sample_contact_name | Anouk,,Emadali
| Sample_contact_email | anouk.emadali@ujf-grenoble.fr
| Sample_contact_institute | Institut Albert Bonniot
| Sample_contact_address | BP170
| Sample_contact_city | Grenoble
| Sample_contact_zip/postal_code | 38042
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1139nnn/GSM1139506/suppl/GSM1139506_raji_shcyclon_MOl_3-8_3_20110311_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE46873
| Sample_data_row_count | 54675
| |
|
GSM1139507 | GPL570 |
|
Raji_shCyclon_rep4
|
Raji_transduced with a sh RNA targeting CYCLON
|
cell line: Raji
cell type: Raji Burkitt lymphoma B cells
transduced with: CYCLON shRNA constructs
|
Gene expression data from Raji cells transduced with a sh RNA targeting CYCLON
|
Sample_geo_accession | GSM1139507
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | May 13 2013
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Stable cell lines have been established using lentiviral transduction of Raji cells with either a control (non-targeting) shRNA sequence or CYCLON shRNA constructs under puromycin selection.
| Sample_growth_protocol_ch1 | Raji cells were cultured in RPMI supplemented with 20 % fœtal bovine serum, sodium pyruvate, non esssential amino acids and penicilllin/streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared using the kit GeneChip IVT express according to manufacturer’s instructions.
| Sample_hyb_protocol | cRNA was hybridized to HG U133 Plus 2.0 microarrays according to manufacturer’s instructions.
| Sample_scan_protocol | Fluorescence intensities were quantified using the GCOS 1.2 software
| Sample_data_processing | The data were analyzed with GeneSpring software (v.11.0, Agilent) using default analysis settings and RMA normalization (with no baseline transormation).
| Sample_platform_id | GPL570
| Sample_contact_name | Anouk,,Emadali
| Sample_contact_email | anouk.emadali@ujf-grenoble.fr
| Sample_contact_institute | Institut Albert Bonniot
| Sample_contact_address | BP170
| Sample_contact_city | Grenoble
| Sample_contact_zip/postal_code | 38042
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1139nnn/GSM1139507/suppl/GSM1139507_raji_shcyclon_MOl_3-8_4_20110311_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE46873
| Sample_data_row_count | 54675
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