Search results for the GEO ID: GSE46922  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1141222 | GPL570 |
|
peripheral blood T-cells of with newly diagnosed ITP 6
|
peripheral blood T-cells of with newly diagnosed ITP
|
disease state: newly diagnosed ITP
tissue: peripheral blood
cell type: T-cell
|
gene expression data from T-cell
|
| Sample_geo_accession | GSM1141222
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | May 14 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were snap frozen in -80 C. Heparin anti-coagulated blood was obtained from each study subject. T-cells were isolated, in brief, peripheral blood mononuclear cells (PBMCs) were separated from the blood immediately after collection, by density gradient centrifugation. After removal of CD14+ cells by magnetic microbeads, T-cells were positively selected using CD3+ magnetic microbeads, according to the manufacturer's recommendations (MACS, Miltenyi Biotec, Surrey, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using Chomczynski's method (Anal Biochem 162, 156-9 (1987))
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Twenty ng of purified RNA was reverse transcribed, amplified and labeled using the Ovation amplification system V2 (NuGEN Technologies Inc, San Carlos, CA) according to the manufacturer's instructions. Five μg of the generated cDNA was fragmented and biotinylated using the Encore biotin module (NuGEN).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on Human U133 Plus 2.0 arrays. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | After hybridisation (according to the Minimum Information About a Microarray Experiment guideline), the arrays were scanned using an Affymetrix confocal laser scanner (Affymetrix, GeneArray scanner GCS3000) and visualised using GeneChip Operating Software (GCOS, Affymetrix).
| | Sample_data_processing | The raw expression signals were preprocessed following the method ofMASS5 (Affymetrix).The robust quantile method was applied to get normalized expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Intawat,,Nookaew
| | Sample_contact_email | intawat@chalmers.se
| | Sample_contact_department | Department of Chemical and Biological Engineering
| | Sample_contact_institute | Chalmers University of Technology
| | Sample_contact_address | Kemivägen 10
| | Sample_contact_city | Göteborg
| | Sample_contact_zip/postal_code | SE-412 96
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1141nnn/GSM1141222/suppl/GSM1141222_New6.CEL.gz
| | Sample_series_id | GSE46922
| | Sample_data_row_count | 54675
| | |
|
GSM1141223 | GPL570 |
|
peripheral blood T-cells of with newly diagnosed ITP 7
|
peripheral blood T-cells of with newly diagnosed ITP
|
disease state: newly diagnosed ITP
tissue: peripheral blood
cell type: T-cell
|
gene expression data from T-cell
|
| Sample_geo_accession | GSM1141223
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | May 14 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were snap frozen in -80 C. Heparin anti-coagulated blood was obtained from each study subject. T-cells were isolated, in brief, peripheral blood mononuclear cells (PBMCs) were separated from the blood immediately after collection, by density gradient centrifugation. After removal of CD14+ cells by magnetic microbeads, T-cells were positively selected using CD3+ magnetic microbeads, according to the manufacturer's recommendations (MACS, Miltenyi Biotec, Surrey, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using Chomczynski's method (Anal Biochem 162, 156-9 (1987))
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Twenty ng of purified RNA was reverse transcribed, amplified and labeled using the Ovation amplification system V2 (NuGEN Technologies Inc, San Carlos, CA) according to the manufacturer's instructions. Five μg of the generated cDNA was fragmented and biotinylated using the Encore biotin module (NuGEN).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on Human U133 Plus 2.0 arrays. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | After hybridisation (according to the Minimum Information About a Microarray Experiment guideline), the arrays were scanned using an Affymetrix confocal laser scanner (Affymetrix, GeneArray scanner GCS3000) and visualised using GeneChip Operating Software (GCOS, Affymetrix).
| | Sample_data_processing | The raw expression signals were preprocessed following the method ofMASS5 (Affymetrix).The robust quantile method was applied to get normalized expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Intawat,,Nookaew
| | Sample_contact_email | intawat@chalmers.se
| | Sample_contact_department | Department of Chemical and Biological Engineering
| | Sample_contact_institute | Chalmers University of Technology
| | Sample_contact_address | Kemivägen 10
| | Sample_contact_city | Göteborg
| | Sample_contact_zip/postal_code | SE-412 96
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1141nnn/GSM1141223/suppl/GSM1141223_New7.CEL.gz
| | Sample_series_id | GSE46922
| | Sample_data_row_count | 54675
| | |
|
GSM1141224 | GPL570 |
|
peripheral blood T-cells of with newly diagnosed ITP 4
|
peripheral blood T-cells of with newly diagnosed ITP
|
disease state: newly diagnosed ITP
tissue: peripheral blood
cell type: T-cell
|
gene expression data from T-cell
|
| Sample_geo_accession | GSM1141224
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | May 14 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were snap frozen in -80 C. Heparin anti-coagulated blood was obtained from each study subject. T-cells were isolated, in brief, peripheral blood mononuclear cells (PBMCs) were separated from the blood immediately after collection, by density gradient centrifugation. After removal of CD14+ cells by magnetic microbeads, T-cells were positively selected using CD3+ magnetic microbeads, according to the manufacturer's recommendations (MACS, Miltenyi Biotec, Surrey, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using Chomczynski's method (Anal Biochem 162, 156-9 (1987))
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Twenty ng of purified RNA was reverse transcribed, amplified and labeled using the Ovation amplification system V2 (NuGEN Technologies Inc, San Carlos, CA) according to the manufacturer's instructions. Five μg of the generated cDNA was fragmented and biotinylated using the Encore biotin module (NuGEN).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on Human U133 Plus 2.0 arrays. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | After hybridisation (according to the Minimum Information About a Microarray Experiment guideline), the arrays were scanned using an Affymetrix confocal laser scanner (Affymetrix, GeneArray scanner GCS3000) and visualised using GeneChip Operating Software (GCOS, Affymetrix).
| | Sample_data_processing | The raw expression signals were preprocessed following the method ofMASS5 (Affymetrix).The robust quantile method was applied to get normalized expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Intawat,,Nookaew
| | Sample_contact_email | intawat@chalmers.se
| | Sample_contact_department | Department of Chemical and Biological Engineering
| | Sample_contact_institute | Chalmers University of Technology
| | Sample_contact_address | Kemivägen 10
| | Sample_contact_city | Göteborg
| | Sample_contact_zip/postal_code | SE-412 96
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1141nnn/GSM1141224/suppl/GSM1141224_New4.CEL.gz
| | Sample_series_id | GSE46922
| | Sample_data_row_count | 54675
| | |
|
GSM1141225 | GPL570 |
|
peripheral blood T-cells of with newly diagnosed ITP 2
|
peripheral blood T-cells of with newly diagnosed ITP
|
disease state: newly diagnosed ITP
tissue: peripheral blood
cell type: T-cell
|
gene expression data from T-cell
|
| Sample_geo_accession | GSM1141225
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | May 14 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were snap frozen in -80 C. Heparin anti-coagulated blood was obtained from each study subject. T-cells were isolated, in brief, peripheral blood mononuclear cells (PBMCs) were separated from the blood immediately after collection, by density gradient centrifugation. After removal of CD14+ cells by magnetic microbeads, T-cells were positively selected using CD3+ magnetic microbeads, according to the manufacturer's recommendations (MACS, Miltenyi Biotec, Surrey, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using Chomczynski's method (Anal Biochem 162, 156-9 (1987))
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Twenty ng of purified RNA was reverse transcribed, amplified and labeled using the Ovation amplification system V2 (NuGEN Technologies Inc, San Carlos, CA) according to the manufacturer's instructions. Five μg of the generated cDNA was fragmented and biotinylated using the Encore biotin module (NuGEN).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on Human U133 Plus 2.0 arrays. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | After hybridisation (according to the Minimum Information About a Microarray Experiment guideline), the arrays were scanned using an Affymetrix confocal laser scanner (Affymetrix, GeneArray scanner GCS3000) and visualised using GeneChip Operating Software (GCOS, Affymetrix).
| | Sample_data_processing | The raw expression signals were preprocessed following the method ofMASS5 (Affymetrix).The robust quantile method was applied to get normalized expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Intawat,,Nookaew
| | Sample_contact_email | intawat@chalmers.se
| | Sample_contact_department | Department of Chemical and Biological Engineering
| | Sample_contact_institute | Chalmers University of Technology
| | Sample_contact_address | Kemivägen 10
| | Sample_contact_city | Göteborg
| | Sample_contact_zip/postal_code | SE-412 96
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1141nnn/GSM1141225/suppl/GSM1141225_New2.CEL.gz
| | Sample_series_id | GSE46922
| | Sample_data_row_count | 54675
| | |
|
GSM1141226 | GPL570 |
|
peripheral blood T-cells of with newly diagnosed ITP 3
|
peripheral blood T-cells of with newly diagnosed ITP
|
disease state: newly diagnosed ITP
tissue: peripheral blood
cell type: T-cell
|
gene expression data from T-cell
|
| Sample_geo_accession | GSM1141226
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | May 14 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were snap frozen in -80 C. Heparin anti-coagulated blood was obtained from each study subject. T-cells were isolated, in brief, peripheral blood mononuclear cells (PBMCs) were separated from the blood immediately after collection, by density gradient centrifugation. After removal of CD14+ cells by magnetic microbeads, T-cells were positively selected using CD3+ magnetic microbeads, according to the manufacturer's recommendations (MACS, Miltenyi Biotec, Surrey, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using Chomczynski's method (Anal Biochem 162, 156-9 (1987))
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Twenty ng of purified RNA was reverse transcribed, amplified and labeled using the Ovation amplification system V2 (NuGEN Technologies Inc, San Carlos, CA) according to the manufacturer's instructions. Five μg of the generated cDNA was fragmented and biotinylated using the Encore biotin module (NuGEN).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on Human U133 Plus 2.0 arrays. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | After hybridisation (according to the Minimum Information About a Microarray Experiment guideline), the arrays were scanned using an Affymetrix confocal laser scanner (Affymetrix, GeneArray scanner GCS3000) and visualised using GeneChip Operating Software (GCOS, Affymetrix).
| | Sample_data_processing | The raw expression signals were preprocessed following the method ofMASS5 (Affymetrix).The robust quantile method was applied to get normalized expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Intawat,,Nookaew
| | Sample_contact_email | intawat@chalmers.se
| | Sample_contact_department | Department of Chemical and Biological Engineering
| | Sample_contact_institute | Chalmers University of Technology
| | Sample_contact_address | Kemivägen 10
| | Sample_contact_city | Göteborg
| | Sample_contact_zip/postal_code | SE-412 96
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1141nnn/GSM1141226/suppl/GSM1141226_New3.CEL.gz
| | Sample_series_id | GSE46922
| | Sample_data_row_count | 54675
| | |
|
GSM1141227 | GPL570 |
|
peripheral blood T-cells of with newly diagnosed ITP 5
|
peripheral blood T-cells of with newly diagnosed ITP
|
disease state: newly diagnosed ITP
tissue: peripheral blood
cell type: T-cell
|
gene expression data from T-cell
|
| Sample_geo_accession | GSM1141227
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | May 14 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were snap frozen in -80 C. Heparin anti-coagulated blood was obtained from each study subject. T-cells were isolated, in brief, peripheral blood mononuclear cells (PBMCs) were separated from the blood immediately after collection, by density gradient centrifugation. After removal of CD14+ cells by magnetic microbeads, T-cells were positively selected using CD3+ magnetic microbeads, according to the manufacturer's recommendations (MACS, Miltenyi Biotec, Surrey, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using Chomczynski's method (Anal Biochem 162, 156-9 (1987))
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Twenty ng of purified RNA was reverse transcribed, amplified and labeled using the Ovation amplification system V2 (NuGEN Technologies Inc, San Carlos, CA) according to the manufacturer's instructions. Five μg of the generated cDNA was fragmented and biotinylated using the Encore biotin module (NuGEN).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on Human U133 Plus 2.0 arrays. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | After hybridisation (according to the Minimum Information About a Microarray Experiment guideline), the arrays were scanned using an Affymetrix confocal laser scanner (Affymetrix, GeneArray scanner GCS3000) and visualised using GeneChip Operating Software (GCOS, Affymetrix).
| | Sample_data_processing | The raw expression signals were preprocessed following the method ofMASS5 (Affymetrix).The robust quantile method was applied to get normalized expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Intawat,,Nookaew
| | Sample_contact_email | intawat@chalmers.se
| | Sample_contact_department | Department of Chemical and Biological Engineering
| | Sample_contact_institute | Chalmers University of Technology
| | Sample_contact_address | Kemivägen 10
| | Sample_contact_city | Göteborg
| | Sample_contact_zip/postal_code | SE-412 96
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1141nnn/GSM1141227/suppl/GSM1141227_New5.CEL.gz
| | Sample_series_id | GSE46922
| | Sample_data_row_count | 54675
| | |
|
GSM1141228 | GPL570 |
|
peripheral blood T-cells of with newly diagnosed ITP 1
|
peripheral blood T-cells of with newly diagnosed ITP
|
disease state: newly diagnosed ITP
tissue: peripheral blood
cell type: T-cell
|
gene expression data from T-cell
|
| Sample_geo_accession | GSM1141228
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | May 14 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were snap frozen in -80 C. Heparin anti-coagulated blood was obtained from each study subject. T-cells were isolated, in brief, peripheral blood mononuclear cells (PBMCs) were separated from the blood immediately after collection, by density gradient centrifugation. After removal of CD14+ cells by magnetic microbeads, T-cells were positively selected using CD3+ magnetic microbeads, according to the manufacturer's recommendations (MACS, Miltenyi Biotec, Surrey, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using Chomczynski's method (Anal Biochem 162, 156-9 (1987))
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Twenty ng of purified RNA was reverse transcribed, amplified and labeled using the Ovation amplification system V2 (NuGEN Technologies Inc, San Carlos, CA) according to the manufacturer's instructions. Five μg of the generated cDNA was fragmented and biotinylated using the Encore biotin module (NuGEN).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on Human U133 Plus 2.0 arrays. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | After hybridisation (according to the Minimum Information About a Microarray Experiment guideline), the arrays were scanned using an Affymetrix confocal laser scanner (Affymetrix, GeneArray scanner GCS3000) and visualised using GeneChip Operating Software (GCOS, Affymetrix).
| | Sample_data_processing | The raw expression signals were preprocessed following the method ofMASS5 (Affymetrix).The robust quantile method was applied to get normalized expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Intawat,,Nookaew
| | Sample_contact_email | intawat@chalmers.se
| | Sample_contact_department | Department of Chemical and Biological Engineering
| | Sample_contact_institute | Chalmers University of Technology
| | Sample_contact_address | Kemivägen 10
| | Sample_contact_city | Göteborg
| | Sample_contact_zip/postal_code | SE-412 96
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1141nnn/GSM1141228/suppl/GSM1141228_New1.CEL.gz
| | Sample_series_id | GSE46922
| | Sample_data_row_count | 54675
| | |
|
GSM1141229 | GPL570 |
|
peripheral blood T-cells Chronic ITP 5
|
peripheral blood T-cells Chronic ITP
|
disease state: Chronic ITP
tissue: peripheral blood
cell type: T-cell
|
gene expression data from T-cell
|
| Sample_geo_accession | GSM1141229
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | May 14 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were snap frozen in -80 C. Heparin anti-coagulated blood was obtained from each study subject. T-cells were isolated, in brief, peripheral blood mononuclear cells (PBMCs) were separated from the blood immediately after collection, by density gradient centrifugation. After removal of CD14+ cells by magnetic microbeads, T-cells were positively selected using CD3+ magnetic microbeads, according to the manufacturer's recommendations (MACS, Miltenyi Biotec, Surrey, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using Chomczynski's method (Anal Biochem 162, 156-9 (1987))
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Twenty ng of purified RNA was reverse transcribed, amplified and labeled using the Ovation amplification system V2 (NuGEN Technologies Inc, San Carlos, CA) according to the manufacturer's instructions. Five μg of the generated cDNA was fragmented and biotinylated using the Encore biotin module (NuGEN).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on Human U133 Plus 2.0 arrays. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | After hybridisation (according to the Minimum Information About a Microarray Experiment guideline), the arrays were scanned using an Affymetrix confocal laser scanner (Affymetrix, GeneArray scanner GCS3000) and visualised using GeneChip Operating Software (GCOS, Affymetrix).
| | Sample_data_processing | The raw expression signals were preprocessed following the method ofMASS5 (Affymetrix).The robust quantile method was applied to get normalized expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Intawat,,Nookaew
| | Sample_contact_email | intawat@chalmers.se
| | Sample_contact_department | Department of Chemical and Biological Engineering
| | Sample_contact_institute | Chalmers University of Technology
| | Sample_contact_address | Kemivägen 10
| | Sample_contact_city | Göteborg
| | Sample_contact_zip/postal_code | SE-412 96
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1141nnn/GSM1141229/suppl/GSM1141229_Chronic5.CEL.gz
| | Sample_series_id | GSE46922
| | Sample_data_row_count | 54675
| | |
|
GSM1141230 | GPL570 |
|
peripheral blood T-cells Chronic ITP 1
|
peripheral blood T-cells Chronic ITP
|
disease state: Chronic ITP
tissue: peripheral blood
cell type: T-cell
|
gene expression data from T-cell
|
| Sample_geo_accession | GSM1141230
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | May 14 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were snap frozen in -80 C. Heparin anti-coagulated blood was obtained from each study subject. T-cells were isolated, in brief, peripheral blood mononuclear cells (PBMCs) were separated from the blood immediately after collection, by density gradient centrifugation. After removal of CD14+ cells by magnetic microbeads, T-cells were positively selected using CD3+ magnetic microbeads, according to the manufacturer's recommendations (MACS, Miltenyi Biotec, Surrey, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using Chomczynski's method (Anal Biochem 162, 156-9 (1987))
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Twenty ng of purified RNA was reverse transcribed, amplified and labeled using the Ovation amplification system V2 (NuGEN Technologies Inc, San Carlos, CA) according to the manufacturer's instructions. Five μg of the generated cDNA was fragmented and biotinylated using the Encore biotin module (NuGEN).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on Human U133 Plus 2.0 arrays. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | After hybridisation (according to the Minimum Information About a Microarray Experiment guideline), the arrays were scanned using an Affymetrix confocal laser scanner (Affymetrix, GeneArray scanner GCS3000) and visualised using GeneChip Operating Software (GCOS, Affymetrix).
| | Sample_data_processing | The raw expression signals were preprocessed following the method ofMASS5 (Affymetrix).The robust quantile method was applied to get normalized expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Intawat,,Nookaew
| | Sample_contact_email | intawat@chalmers.se
| | Sample_contact_department | Department of Chemical and Biological Engineering
| | Sample_contact_institute | Chalmers University of Technology
| | Sample_contact_address | Kemivägen 10
| | Sample_contact_city | Göteborg
| | Sample_contact_zip/postal_code | SE-412 96
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1141nnn/GSM1141230/suppl/GSM1141230_Chronic1.CEL.gz
| | Sample_series_id | GSE46922
| | Sample_data_row_count | 54675
| | |
|
GSM1141231 | GPL570 |
|
peripheral blood T-cells Chronic ITP 2
|
peripheral blood T-cells Chronic ITP
|
disease state: Chronic ITP
tissue: peripheral blood
cell type: T-cell
|
gene expression data from T-cell
|
| Sample_geo_accession | GSM1141231
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | May 14 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were snap frozen in -80 C. Heparin anti-coagulated blood was obtained from each study subject. T-cells were isolated, in brief, peripheral blood mononuclear cells (PBMCs) were separated from the blood immediately after collection, by density gradient centrifugation. After removal of CD14+ cells by magnetic microbeads, T-cells were positively selected using CD3+ magnetic microbeads, according to the manufacturer's recommendations (MACS, Miltenyi Biotec, Surrey, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using Chomczynski's method (Anal Biochem 162, 156-9 (1987))
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Twenty ng of purified RNA was reverse transcribed, amplified and labeled using the Ovation amplification system V2 (NuGEN Technologies Inc, San Carlos, CA) according to the manufacturer's instructions. Five μg of the generated cDNA was fragmented and biotinylated using the Encore biotin module (NuGEN).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on Human U133 Plus 2.0 arrays. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | After hybridisation (according to the Minimum Information About a Microarray Experiment guideline), the arrays were scanned using an Affymetrix confocal laser scanner (Affymetrix, GeneArray scanner GCS3000) and visualised using GeneChip Operating Software (GCOS, Affymetrix).
| | Sample_data_processing | The raw expression signals were preprocessed following the method ofMASS5 (Affymetrix).The robust quantile method was applied to get normalized expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Intawat,,Nookaew
| | Sample_contact_email | intawat@chalmers.se
| | Sample_contact_department | Department of Chemical and Biological Engineering
| | Sample_contact_institute | Chalmers University of Technology
| | Sample_contact_address | Kemivägen 10
| | Sample_contact_city | Göteborg
| | Sample_contact_zip/postal_code | SE-412 96
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1141nnn/GSM1141231/suppl/GSM1141231_Chronic2.CEL.gz
| | Sample_series_id | GSE46922
| | Sample_data_row_count | 54675
| | |
|
GSM1141232 | GPL570 |
|
peripheral blood T-cells Chronic ITP 6
|
peripheral blood T-cells Chronic ITP
|
disease state: Chronic ITP
tissue: peripheral blood
cell type: T-cell
|
gene expression data from T-cell
|
| Sample_geo_accession | GSM1141232
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | May 14 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were snap frozen in -80 C. Heparin anti-coagulated blood was obtained from each study subject. T-cells were isolated, in brief, peripheral blood mononuclear cells (PBMCs) were separated from the blood immediately after collection, by density gradient centrifugation. After removal of CD14+ cells by magnetic microbeads, T-cells were positively selected using CD3+ magnetic microbeads, according to the manufacturer's recommendations (MACS, Miltenyi Biotec, Surrey, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using Chomczynski's method (Anal Biochem 162, 156-9 (1987))
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Twenty ng of purified RNA was reverse transcribed, amplified and labeled using the Ovation amplification system V2 (NuGEN Technologies Inc, San Carlos, CA) according to the manufacturer's instructions. Five μg of the generated cDNA was fragmented and biotinylated using the Encore biotin module (NuGEN).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on Human U133 Plus 2.0 arrays. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | After hybridisation (according to the Minimum Information About a Microarray Experiment guideline), the arrays were scanned using an Affymetrix confocal laser scanner (Affymetrix, GeneArray scanner GCS3000) and visualised using GeneChip Operating Software (GCOS, Affymetrix).
| | Sample_data_processing | The raw expression signals were preprocessed following the method ofMASS5 (Affymetrix).The robust quantile method was applied to get normalized expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Intawat,,Nookaew
| | Sample_contact_email | intawat@chalmers.se
| | Sample_contact_department | Department of Chemical and Biological Engineering
| | Sample_contact_institute | Chalmers University of Technology
| | Sample_contact_address | Kemivägen 10
| | Sample_contact_city | Göteborg
| | Sample_contact_zip/postal_code | SE-412 96
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1141nnn/GSM1141232/suppl/GSM1141232_Chronic6.CEL.gz
| | Sample_series_id | GSE46922
| | Sample_data_row_count | 54675
| | |
|
GSM1141233 | GPL570 |
|
peripheral blood T-cells Chronic ITP 4
|
peripheral blood T-cells Chronic ITP
|
disease state: Chronic ITP
tissue: peripheral blood
cell type: T-cell
|
gene expression data from T-cell
|
| Sample_geo_accession | GSM1141233
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | May 14 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were snap frozen in -80 C. Heparin anti-coagulated blood was obtained from each study subject. T-cells were isolated, in brief, peripheral blood mononuclear cells (PBMCs) were separated from the blood immediately after collection, by density gradient centrifugation. After removal of CD14+ cells by magnetic microbeads, T-cells were positively selected using CD3+ magnetic microbeads, according to the manufacturer's recommendations (MACS, Miltenyi Biotec, Surrey, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using Chomczynski's method (Anal Biochem 162, 156-9 (1987))
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Twenty ng of purified RNA was reverse transcribed, amplified and labeled using the Ovation amplification system V2 (NuGEN Technologies Inc, San Carlos, CA) according to the manufacturer's instructions. Five μg of the generated cDNA was fragmented and biotinylated using the Encore biotin module (NuGEN).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on Human U133 Plus 2.0 arrays. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | After hybridisation (according to the Minimum Information About a Microarray Experiment guideline), the arrays were scanned using an Affymetrix confocal laser scanner (Affymetrix, GeneArray scanner GCS3000) and visualised using GeneChip Operating Software (GCOS, Affymetrix).
| | Sample_data_processing | The raw expression signals were preprocessed following the method ofMASS5 (Affymetrix).The robust quantile method was applied to get normalized expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Intawat,,Nookaew
| | Sample_contact_email | intawat@chalmers.se
| | Sample_contact_department | Department of Chemical and Biological Engineering
| | Sample_contact_institute | Chalmers University of Technology
| | Sample_contact_address | Kemivägen 10
| | Sample_contact_city | Göteborg
| | Sample_contact_zip/postal_code | SE-412 96
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1141nnn/GSM1141233/suppl/GSM1141233_Chronic4.CEL.gz
| | Sample_series_id | GSE46922
| | Sample_data_row_count | 54675
| | |
|
GSM1141234 | GPL570 |
|
peripheral blood T-cells Chronic ITP 3
|
peripheral blood T-cells Chronic ITP
|
disease state: Chronic ITP
tissue: peripheral blood
cell type: T-cell
|
gene expression data from T-cell
|
| Sample_geo_accession | GSM1141234
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | May 14 2013
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were snap frozen in -80 C. Heparin anti-coagulated blood was obtained from each study subject. T-cells were isolated, in brief, peripheral blood mononuclear cells (PBMCs) were separated from the blood immediately after collection, by density gradient centrifugation. After removal of CD14+ cells by magnetic microbeads, T-cells were positively selected using CD3+ magnetic microbeads, according to the manufacturer's recommendations (MACS, Miltenyi Biotec, Surrey, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using Chomczynski's method (Anal Biochem 162, 156-9 (1987))
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Twenty ng of purified RNA was reverse transcribed, amplified and labeled using the Ovation amplification system V2 (NuGEN Technologies Inc, San Carlos, CA) according to the manufacturer's instructions. Five μg of the generated cDNA was fragmented and biotinylated using the Encore biotin module (NuGEN).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on Human U133 Plus 2.0 arrays. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | After hybridisation (according to the Minimum Information About a Microarray Experiment guideline), the arrays were scanned using an Affymetrix confocal laser scanner (Affymetrix, GeneArray scanner GCS3000) and visualised using GeneChip Operating Software (GCOS, Affymetrix).
| | Sample_data_processing | The raw expression signals were preprocessed following the method ofMASS5 (Affymetrix).The robust quantile method was applied to get normalized expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Intawat,,Nookaew
| | Sample_contact_email | intawat@chalmers.se
| | Sample_contact_department | Department of Chemical and Biological Engineering
| | Sample_contact_institute | Chalmers University of Technology
| | Sample_contact_address | Kemivägen 10
| | Sample_contact_city | Göteborg
| | Sample_contact_zip/postal_code | SE-412 96
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1141nnn/GSM1141234/suppl/GSM1141234_Chronic3.CEL.gz
| | Sample_series_id | GSE46922
| | Sample_data_row_count | 54675
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
