Result

Search results for the GEO ID: GSE46925

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM1141247

GPL570

Control_234

MDA-MB-231_control serum

cell line: MDA-MB-231 cell type: human breast adenocarcinoma cells treated with: serum from sedentary control breast cancer patient

Sample_geo_accession	GSM1141247
Sample_status	Public on Aug 01 2013
Sample_submission_date	May 14 2013
Sample_last_update_date	Aug 01 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Patient serum was then added to the wells at a 10% final concentration and RNA was harvested using Qiagen RNeasy kit after 4 days.
Sample_growth_protocol_ch1	MDA-MB-231 cells were plated at 250,000 cells/well with 10% FBS in 6-well plates and left overnight to adhere. Media was suctioned off and replaced with serum free media. Patient serum was then added to the wells at a 10% final concentration and RNA was harvested using Qiagen RNeasy kit after 4 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA were assessed for quality with Agilent 2100 Bioanalyzer G2939A (Agilent Technologies,Santa Clara, CA)) and Nanodrop 8000 spectrophotometer (Thermo Scientific/Nanodrop, Wilmington, DE).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA, hybridized to Affymetrix Human Genome U133 Plus 2.0 GeneChip® arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450 and scanned with Affymetrix GeneChip® Scanner 7G according to standard Affymetrix GeneChip® Hybridization, Wash, and Stain protocols. (Affymetrix, Santa Clara,CA).
Sample_hyb_protocol	Gene Chip hG-133 plus 2.0 (Affymetrix)
Sample_scan_protocol	Affymetrix GeneChip® Scanner 7G
Sample_data_processing	Raw data was background corrected with RMA, Log2 transformed, quantile normalized and summarized by median polish using the Affy package (v.1.36.1) in Bioconductor(v.2.15.2).
Sample_data_processing	Log2 RMA, quantile normalization, and summarized by median polish using Bioconductor (2.15.2).
Sample_platform_id	GPL570
Sample_contact_name	Jeff,,Jasper
Sample_contact_laboratory	McDonnell
Sample_contact_department	Pharmacology and Cancer Biology
Sample_contact_institute	Duke University
Sample_contact_address	Box 3813
Sample_contact_city	Durham
Sample_contact_state	NC
Sample_contact_zip/postal_code	27710
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1141nnn/GSM1141247/suppl/GSM1141247_3303_12350_40183_234C_HG-U133+Plus+2.CEL.gz
Sample_series_id	GSE46925
Sample_data_row_count	54675

GSM1141248

GPL570

Control_256

MDA-MB-231_control serum

cell line: MDA-MB-231 cell type: human breast adenocarcinoma cells treated with: serum from sedentary control breast cancer patient

Sample_geo_accession	GSM1141248
Sample_status	Public on Aug 01 2013
Sample_submission_date	May 14 2013
Sample_last_update_date	Aug 01 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Patient serum was then added to the wells at a 10% final concentration and RNA was harvested using Qiagen RNeasy kit after 4 days.
Sample_growth_protocol_ch1	MDA-MB-231 cells were plated at 250,000 cells/well with 10% FBS in 6-well plates and left overnight to adhere. Media was suctioned off and replaced with serum free media. Patient serum was then added to the wells at a 10% final concentration and RNA was harvested using Qiagen RNeasy kit after 4 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA were assessed for quality with Agilent 2100 Bioanalyzer G2939A (Agilent Technologies,Santa Clara, CA)) and Nanodrop 8000 spectrophotometer (Thermo Scientific/Nanodrop, Wilmington, DE).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA, hybridized to Affymetrix Human Genome U133 Plus 2.0 GeneChip® arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450 and scanned with Affymetrix GeneChip® Scanner 7G according to standard Affymetrix GeneChip® Hybridization, Wash, and Stain protocols. (Affymetrix, Santa Clara,CA).
Sample_hyb_protocol	Gene Chip hG-133 plus 2.0 (Affymetrix)
Sample_scan_protocol	Affymetrix GeneChip® Scanner 7G
Sample_data_processing	Raw data was background corrected with RMA, Log2 transformed, quantile normalized and summarized by median polish using the Affy package (v.1.36.1) in Bioconductor(v.2.15.2).
Sample_data_processing	Log2 RMA, quantile normalization, and summarized by median polish using Bioconductor (2.15.2).
Sample_platform_id	GPL570
Sample_contact_name	Jeff,,Jasper
Sample_contact_laboratory	McDonnell
Sample_contact_department	Pharmacology and Cancer Biology
Sample_contact_institute	Duke University
Sample_contact_address	Box 3813
Sample_contact_city	Durham
Sample_contact_state	NC
Sample_contact_zip/postal_code	27710
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1141nnn/GSM1141248/suppl/GSM1141248_3303_12350_40182_256C_HG-U133+Plus+2.CEL.gz
Sample_series_id	GSE46925
Sample_data_row_count	54675

GSM1141249

GPL570

Control_275

MDA-MB-231_control serum

cell line: MDA-MB-231 cell type: human breast adenocarcinoma cells treated with: serum from sedentary control breast cancer patient

Sample_geo_accession	GSM1141249
Sample_status	Public on Aug 01 2013
Sample_submission_date	May 14 2013
Sample_last_update_date	Aug 01 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Patient serum was then added to the wells at a 10% final concentration and RNA was harvested using Qiagen RNeasy kit after 4 days.
Sample_growth_protocol_ch1	MDA-MB-231 cells were plated at 250,000 cells/well with 10% FBS in 6-well plates and left overnight to adhere. Media was suctioned off and replaced with serum free media. Patient serum was then added to the wells at a 10% final concentration and RNA was harvested using Qiagen RNeasy kit after 4 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA were assessed for quality with Agilent 2100 Bioanalyzer G2939A (Agilent Technologies,Santa Clara, CA)) and Nanodrop 8000 spectrophotometer (Thermo Scientific/Nanodrop, Wilmington, DE).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA, hybridized to Affymetrix Human Genome U133 Plus 2.0 GeneChip® arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450 and scanned with Affymetrix GeneChip® Scanner 7G according to standard Affymetrix GeneChip® Hybridization, Wash, and Stain protocols. (Affymetrix, Santa Clara,CA).
Sample_hyb_protocol	Gene Chip hG-133 plus 2.0 (Affymetrix)
Sample_scan_protocol	Affymetrix GeneChip® Scanner 7G
Sample_data_processing	Raw data was background corrected with RMA, Log2 transformed, quantile normalized and summarized by median polish using the Affy package (v.1.36.1) in Bioconductor(v.2.15.2).
Sample_data_processing	Log2 RMA, quantile normalization, and summarized by median polish using Bioconductor (2.15.2).
Sample_platform_id	GPL570
Sample_contact_name	Jeff,,Jasper
Sample_contact_laboratory	McDonnell
Sample_contact_department	Pharmacology and Cancer Biology
Sample_contact_institute	Duke University
Sample_contact_address	Box 3813
Sample_contact_city	Durham
Sample_contact_state	NC
Sample_contact_zip/postal_code	27710
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1141nnn/GSM1141249/suppl/GSM1141249_3303_12350_40184_275C_HG-U133+Plus+2.CEL.gz
Sample_series_id	GSE46925
Sample_data_row_count	54675

GSM1141250

GPL570

Exercise_245

MDA-MB-231_exercise serum

cell line: MDA-MB-231 cell type: human breast adenocarcinoma cells treated with: serum from breast cancer patients exposed to aerobic training

Sample_geo_accession	GSM1141250
Sample_status	Public on Aug 01 2013
Sample_submission_date	May 14 2013
Sample_last_update_date	Aug 01 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Patient serum was then added to the wells at a 10% final concentration and RNA was harvested using Qiagen RNeasy kit after 4 days.
Sample_growth_protocol_ch1	MDA-MB-231 cells were plated at 250,000 cells/well with 10% FBS in 6-well plates and left overnight to adhere. Media was suctioned off and replaced with serum free media. Patient serum was then added to the wells at a 10% final concentration and RNA was harvested using Qiagen RNeasy kit after 4 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA were assessed for quality with Agilent 2100 Bioanalyzer G2939A (Agilent Technologies,Santa Clara, CA)) and Nanodrop 8000 spectrophotometer (Thermo Scientific/Nanodrop, Wilmington, DE).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA, hybridized to Affymetrix Human Genome U133 Plus 2.0 GeneChip® arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450 and scanned with Affymetrix GeneChip® Scanner 7G according to standard Affymetrix GeneChip® Hybridization, Wash, and Stain protocols. (Affymetrix, Santa Clara,CA).
Sample_hyb_protocol	Gene Chip hG-133 plus 2.0 (Affymetrix)
Sample_scan_protocol	Affymetrix GeneChip® Scanner 7G
Sample_data_processing	Raw data was background corrected with RMA, Log2 transformed, quantile normalized and summarized by median polish using the Affy package (v.1.36.1) in Bioconductor(v.2.15.2).
Sample_data_processing	Log2 RMA, quantile normalization, and summarized by median polish using Bioconductor (2.15.2).
Sample_platform_id	GPL570
Sample_contact_name	Jeff,,Jasper
Sample_contact_laboratory	McDonnell
Sample_contact_department	Pharmacology and Cancer Biology
Sample_contact_institute	Duke University
Sample_contact_address	Box 3813
Sample_contact_city	Durham
Sample_contact_state	NC
Sample_contact_zip/postal_code	27710
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1141nnn/GSM1141250/suppl/GSM1141250_3303_12350_40179_245E_HG-U133+Plus+2.CEL.gz
Sample_series_id	GSE46925
Sample_data_row_count	54675

GSM1141251

GPL570

Exercise_258

MDA-MB-231_exercise serum

cell line: MDA-MB-231 cell type: human breast adenocarcinoma cells treated with: serum from breast cancer patients exposed to aerobic training

Sample_geo_accession	GSM1141251
Sample_status	Public on Aug 01 2013
Sample_submission_date	May 14 2013
Sample_last_update_date	Aug 01 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Patient serum was then added to the wells at a 10% final concentration and RNA was harvested using Qiagen RNeasy kit after 4 days.
Sample_growth_protocol_ch1	MDA-MB-231 cells were plated at 250,000 cells/well with 10% FBS in 6-well plates and left overnight to adhere. Media was suctioned off and replaced with serum free media. Patient serum was then added to the wells at a 10% final concentration and RNA was harvested using Qiagen RNeasy kit after 4 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA were assessed for quality with Agilent 2100 Bioanalyzer G2939A (Agilent Technologies,Santa Clara, CA)) and Nanodrop 8000 spectrophotometer (Thermo Scientific/Nanodrop, Wilmington, DE).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA, hybridized to Affymetrix Human Genome U133 Plus 2.0 GeneChip® arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450 and scanned with Affymetrix GeneChip® Scanner 7G according to standard Affymetrix GeneChip® Hybridization, Wash, and Stain protocols. (Affymetrix, Santa Clara,CA).
Sample_hyb_protocol	Gene Chip hG-133 plus 2.0 (Affymetrix)
Sample_scan_protocol	Affymetrix GeneChip® Scanner 7G
Sample_data_processing	Raw data was background corrected with RMA, Log2 transformed, quantile normalized and summarized by median polish using the Affy package (v.1.36.1) in Bioconductor(v.2.15.2).
Sample_data_processing	Log2 RMA, quantile normalization, and summarized by median polish using Bioconductor (2.15.2).
Sample_platform_id	GPL570
Sample_contact_name	Jeff,,Jasper
Sample_contact_laboratory	McDonnell
Sample_contact_department	Pharmacology and Cancer Biology
Sample_contact_institute	Duke University
Sample_contact_address	Box 3813
Sample_contact_city	Durham
Sample_contact_state	NC
Sample_contact_zip/postal_code	27710
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1141nnn/GSM1141251/suppl/GSM1141251_3303_12350_40178_258E_HG-U133+Plus+2.CEL.gz
Sample_series_id	GSE46925
Sample_data_row_count	54675

GSM1141252

GPL570

Exercise_298

MDA-MB-231_exercise serum

cell line: MDA-MB-231 cell type: human breast adenocarcinoma cells treated with: serum from breast cancer patients exposed to aerobic training

Sample_geo_accession	GSM1141252
Sample_status	Public on Aug 01 2013
Sample_submission_date	May 14 2013
Sample_last_update_date	Aug 01 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Patient serum was then added to the wells at a 10% final concentration and RNA was harvested using Qiagen RNeasy kit after 4 days.
Sample_growth_protocol_ch1	MDA-MB-231 cells were plated at 250,000 cells/well with 10% FBS in 6-well plates and left overnight to adhere. Media was suctioned off and replaced with serum free media. Patient serum was then added to the wells at a 10% final concentration and RNA was harvested using Qiagen RNeasy kit after 4 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA were assessed for quality with Agilent 2100 Bioanalyzer G2939A (Agilent Technologies,Santa Clara, CA)) and Nanodrop 8000 spectrophotometer (Thermo Scientific/Nanodrop, Wilmington, DE).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA, hybridized to Affymetrix Human Genome U133 Plus 2.0 GeneChip® arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450 and scanned with Affymetrix GeneChip® Scanner 7G according to standard Affymetrix GeneChip® Hybridization, Wash, and Stain protocols. (Affymetrix, Santa Clara,CA).
Sample_hyb_protocol	Gene Chip hG-133 plus 2.0 (Affymetrix)
Sample_scan_protocol	Affymetrix GeneChip® Scanner 7G
Sample_data_processing	Raw data was background corrected with RMA, Log2 transformed, quantile normalized and summarized by median polish using the Affy package (v.1.36.1) in Bioconductor(v.2.15.2).
Sample_data_processing	Log2 RMA, quantile normalization, and summarized by median polish using Bioconductor (2.15.2).
Sample_platform_id	GPL570
Sample_contact_name	Jeff,,Jasper
Sample_contact_laboratory	McDonnell
Sample_contact_department	Pharmacology and Cancer Biology
Sample_contact_institute	Duke University
Sample_contact_address	Box 3813
Sample_contact_city	Durham
Sample_contact_state	NC
Sample_contact_zip/postal_code	27710
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1141nnn/GSM1141252/suppl/GSM1141252_3303_12350_40177_298E_HG-U133+Plus+2.CEL.gz
Sample_series_id	GSE46925
Sample_data_row_count	54675

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